Rapid Detection and Counting of Bacteria Using Impact Electrochemical Impedance Spectroscopy DOI
Ashish Kumar Shukla, Jayanta Sarmah Boruah, Seungkyung Park

et al.

The Journal of Physical Chemistry C, Journal Year: 2024, Volume and Issue: 128(32), P. 13458 - 13463

Published: Aug. 7, 2024

Detection and quantification of pathogenic bacteria remain critical amidst the recent focus on viruses as bacterial contamination continues to contribute significantly hospitalizations fatalities globally. With rising antibiotic resistance exacerbating challenge, simple cost-effective approaches are needed for prompt identification bacteria. Conventional methods, while effective, often entail time-consuming processes or require sophisticated equipment, limiting their utility in resource-limited settings. In this study, we employed impact electrochemical impedance spectroscopy (IEIS) rapid detection counting at exceptionally low concentrations. The technique measures changes spectra caused by electrode surfaces, providing a label-free method. We demonstrated two types (Escherichia coli Bacillus subtilis) using gold ultramicroelectrodes (Au-UME). observed significant shifts corresponding concentrations 100 CFU/mL E. 1000 B. subtilis. Our results show that charge-transfer (Rct) increases with concentration, attributed blockage redox probe oxidation Au-UME surface. This change allows quantitative analysis presence. sensitivity versatility our method enhance detection, offering advantage over traditional techniques. study validates IEIS robust tool detecting bacteria, addressing infection control challenges. Further research area promises advance label-free, sensitive, real-time technique.

Language: Английский

Rapid Detection and Counting of Bacteria Using Impact Electrochemical Impedance Spectroscopy DOI
Ashish Kumar Shukla, Jayanta Sarmah Boruah, Seungkyung Park

et al.

The Journal of Physical Chemistry C, Journal Year: 2024, Volume and Issue: 128(32), P. 13458 - 13463

Published: Aug. 7, 2024

Detection and quantification of pathogenic bacteria remain critical amidst the recent focus on viruses as bacterial contamination continues to contribute significantly hospitalizations fatalities globally. With rising antibiotic resistance exacerbating challenge, simple cost-effective approaches are needed for prompt identification bacteria. Conventional methods, while effective, often entail time-consuming processes or require sophisticated equipment, limiting their utility in resource-limited settings. In this study, we employed impact electrochemical impedance spectroscopy (IEIS) rapid detection counting at exceptionally low concentrations. The technique measures changes spectra caused by electrode surfaces, providing a label-free method. We demonstrated two types (Escherichia coli Bacillus subtilis) using gold ultramicroelectrodes (Au-UME). observed significant shifts corresponding concentrations 100 CFU/mL E. 1000 B. subtilis. Our results show that charge-transfer (Rct) increases with concentration, attributed blockage redox probe oxidation Au-UME surface. This change allows quantitative analysis presence. sensitivity versatility our method enhance detection, offering advantage over traditional techniques. study validates IEIS robust tool detecting bacteria, addressing infection control challenges. Further research area promises advance label-free, sensitive, real-time technique.

Language: Английский

Citations

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