iScience,
Journal Year:
2025,
Volume and Issue:
28(2), P. 111763 - 111763
Published: Jan. 7, 2025
Recent
years
have
witnessed
tremendous
advances
in
wearable
sensors,
which
play
an
essential
role
personalized
healthcare
for
their
ability
real-time
sensing
and
detection
of
human
health
information.
Nanozymes,
capable
mimicking
the
functions
natural
enzymes
addressing
limitations,
possess
unique
advantages
such
as
structural
stability,
low
cost,
ease
mass
production,
making
them
particularly
beneficial
constructing
recognition
units
biosensors.
In
this
review,
we
aim
to
delineate
latest
advancements
nanozymes
development
biosensors,
focusing
on
key
developments
nanozyme
immobilization
strategies,
technologies,
biomedical
applications.
The
review
also
highlights
current
challenges
future
perspectives.
Ultimately,
it
aims
provide
insights
research
endeavors
rapidly
evolving
area.
Diagnosis
of
oxidative
stress
is
essential
to
avoiding
serious
life-threatening
situations.
Hydrogen
peroxide
(H2O2)
a
potential
biomarker
stress.
Herein,
we
introduce
reagent-free
nanoscale
approach
for
the
colorimetric
detection
urinary
H2O2
utilizing
dextran-gold
nanoparticles
(dAuNPs).
The
plasmonic
properties
these
are
central
their
function,
leveraging
high
surface
area
and
tunable
optical
characteristics
sensitive
detection.
We
transformed
colloidal
dAuNPs
solution
into
two
formats:
as
tablet
(dAuNPs-Tablet)
or
impregnated
on
cotton
swab
(dAuNPs-Swab).
assay
generates
hydroxyl
radical
(•OH)
from
via
Fenton
reaction,
followed
by
nanoscale-driven
using
sensors.
In
presence
in
sample,
red
color
turns
blue
due
salt-induced
aggregation.
transition
observed
•OH-assisted
degradation
dextran
layer
around
dAuNPs,
leading
loss
stability
subsequent
aggregation
dAuNPs.
Sodium
chloride
acts
aggregating
agent,
enhancing
interactions.
limit
artificial
urine
found
be
50
μM
sensor
100
sensor.
more
stable
compared
which
gradually
loses
stability,
after
one
month,
with
approximately
40%
within
three
months.
Interference
studies
demonstrate
selectivity
both
platforms
Notably,
investigated
levels
human
samples
healthy
volunteers
(both
female
male)
before
green
tea
consumption.
decrease
level
postgreen
consumption
suggests
role
antioxidants
mitigating
utilization
nanoprobes
our
research
not
only
enhances
understanding
dynamics
but
also
drives
advancements
point-of-care
platforms,
offering
enhanced
portability
ease
use
nanoprobes.
These
open
exciting
avenues
healthcare
diagnosis.
Electroanalysis,
Journal Year:
2025,
Volume and Issue:
37(3)
Published: March 1, 2025
The
abnormal
expression
of
hydrogen
peroxide
(H
2
O
)
in
living
cells
is
closely
related
to
the
occurrence
and
development
tumor
diseases.
It
a
kind
potential
marker
for
diagnosis
treatment
Therefore,
it
very
meaningful
develop
high
selective
sensitive
method
real‐time
detecting
H
released
from
cancer
cells.
Herein,
an
AgCl
cube/porous
carbon
nanotube
composite
nanomaterials
was
successfully
fabricated
employed
construct
non‐enzymatic
electrochemical
sensor.
Test
results
showed
that
proposed
sensor
displayed
sensitivity
with
detection
limit
5.3
×
10
−9
mol/L
(S/N
=
3).
Importantly,
can
accurately
analyze
milk
samples
achieve
determination
secreted
In
addition,
established
exhibited
good
stability
anti‐interference
ability.
This
strategy
offers
way
diagnose
