Current hydrogel advances in physicochemical and biological response-driven biomedical application diversity

Signal Transduction and Targeted Therapy, Journal Year: 2021, Volume and Issue: 6(1)

Published: Dec. 16, 2021

Hydrogel is a type of versatile platform with various biomedical applications after rational structure and functional design that leverages on material engineering to modulate its physicochemical properties (e.g., stiffness, pore size, viscoelasticity, microarchitecture, degradability, ligand presentation, stimulus-responsive properties, etc.) influence cell signaling cascades fate. In the past few decades, plethora pioneering studies have been implemented explore cell-hydrogel matrix interactions figure out underlying mechanisms, paving way lab-to-clinic translation hydrogel-based therapies. this review, we first introduced hydrogels their fabrication approaches concisely. Subsequently, comprehensive description deep discussion were elucidated, wherein influences different behaviors cellular events highlighted. These or included integrin clustering, focal adhesion (FA) complex accumulation activation, cytoskeleton rearrangement, protein cyto-nuclei shuttling activation Yes-associated (YAP), catenin, etc.), compartment reorganization, gene expression, further biology modulation spreading, migration, proliferation, lineage commitment, etc.). Based them, current in vitro vivo hydrogel mainly covered diseases models, delivery protocols for tissue regeneration disease therapy, smart drug carrier, bioimaging, biosensor, conductive wearable/implantable biodevices, etc. summarized discussed. More significantly, clinical potential trials presented, accompanied which remaining challenges future perspectives field emphasized. Collectively, insights review will shed light principles new understand processes, are available providing significant indications serving broad range applications.

Language: Английский

---

Hydrogels: Properties and Applications in Biomedicine

Molecules, Journal Year: 2022, Volume and Issue: 27(9), P. 2902 - 2902

Published: May 2, 2022

Hydrogels are crosslinked polymer chains with three-dimensional (3D) network structures, which can absorb relatively large amounts of fluid. Because the high water content, soft structure, and porosity hydrogels, they closely resemble living tissues. Research in recent years shows that hydrogels have been applied various fields, such as agriculture, biomaterials, food industry, drug delivery, tissue engineering, regenerative medicine. Along underlying technology improvements hydrogel development, be expected to more fields. Although not all good biodegradability biocompatibility, synthetic (polyvinyl alcohol, polyacrylamide, polyethylene glycol etc.), their biocompatibility adjusted by modification functional group or incorporation natural polymers. Hence, scientists still interested biomedical applications due creative adjustability for different uses. In this review, we first introduce basic information classification, synthesis. Then, further describe 3D cell cultures, wound dressing, engineering.

Language: Английский

---

Hydrogel scaffolds for tissue engineering: the importance of polymer choice

Polymer Chemistry, Journal Year: 2019, Volume and Issue: 11(2), P. 184 - 219

Published: Sept. 25, 2019

We explore the design and synthesis of hydrogel scaffolds for tissue engineering from perspective underlying polymer chemistry. The key polymers, properties architectures used, their effect on growth are discussed.

Language: Английский

---

Advances and challenges in stem cell culture

Colloids and Surfaces B Biointerfaces, Journal Year: 2017, Volume and Issue: 159, P. 62 - 77

Published: July 27, 2017

Stem cells (SCs) hold great promise for cell therapy, tissue engineering, and regenerative medicine as well pharmaceutical biotechnological applications. They have the capacity to self-renew ability differentiate into specialized types depending upon their source of isolation. However, use SCs clinical applications requires a high quality quantity cells. This necessitates large-scale expansion followed by efficient homogeneous differentiation functional derivatives. Traditional methods maintenance rely on two-dimensional (2-D) culturing techniques using plastic culture plates xenogenic media. These provide limited tend lose clonal long-term passaging. Recently, new approaches emphasized three-dimensional (3-D) growth mimic in vivo environment. review provides comprehensive compendium recent advancements 2-D 3-D involving spheroids, biomaterials, bioreactors. In addition, potential challenges achieve billion-fold are discussed.

Language: Английский

---

Functional Hydrogels With Tunable Structures and Properties for Tissue Engineering Applications

Frontiers in Chemistry, Journal Year: 2018, Volume and Issue: 6

Published: Oct. 22, 2018

Tissue engineering (TE) has been used as an attractive and efficient process to restore the original tissue structures functions through combination of biodegradable scaffolds, seeded cells, biological factors. As a unique type hydrogels have frequently for TE because their similar 3D native extracellular matrix (ECM), well tunable biochemical biophysical properties control cell such migration, adhesion, proliferation, differentiation. Various types prepared from naturally derived biomaterials, synthetic polymers, or combination, showing promise in TE. This review summarizes very recent progress applications. The strategies tuning properties, are first introduced. Their influences on promotive effects regeneration then highlighted.

Language: Английский

---

Photoreceptor Outer Segment-like Structures in Long-Term 3D Retinas from Human Pluripotent Stem Cells

Scientific Reports, Journal Year: 2017, Volume and Issue: 7(1)

Published: April 4, 2017

The retinal degenerative diseases, which together constitute a leading cause of hereditary blindness worldwide, are largely untreatable. Development reliable methods to culture complex tissues from human pluripotent stem cells (hPSCs) could offer means study development, provide platform investigate the mechanisms degeneration and screen for neuroprotective compounds, basis cell-based therapeutic strategies. In this study, we describe an in vitro method by hPSCs can be differentiated into 3D retinas with at least some important features reminiscent mature retina, including exuberant outgrowth outer segment-like structures synaptic ribbons, photoreceptor neurotransmitter expression, membrane conductances vesicle release properties consistent possible function. advanced reported here support notion that retina cups serve as model studying development allow more robust modeling disease vitro.

Language: Английский

---

Accelerated and Improved Differentiation of Retinal Organoids from Pluripotent Stem Cells in Rotating-Wall Vessel Bioreactors

Stem Cell Reports, Journal Year: 2017, Volume and Issue: 10(1), P. 300 - 313

Published: Dec. 7, 2017

Pluripotent stem cells can be differentiated into 3D retinal organoids, with major cell types self-patterning a polarized, laminated architecture. In static cultures, organoid development may hindered by limitations in diffusion of oxygen and nutrients. Herein, we report bioprocess using rotating-wall vessel (RWV) bioreactors to culture organoids derived from mouse pluripotent cells. Organoids RWV demonstrate enhanced proliferation, well-defined morphology improved differentiation neurons including ganglion S-cone photoreceptors. Furthermore, at day 25 (D25) reveal similar maturation transcriptome profile as those D32 culture, closely recapitulating spatiotemporal postnatal 6 retina vivo. Interestingly, however, do not differentiate further under any vitro condition tested here, suggesting additional requirements for functional maturation. Our studies that accelerate improve growth modeling disease evaluation therapies.

Language: Английский

---

Looking into the Future: Toward Advanced 3D Biomaterials for Stem‐Cell‐Based Regenerative Medicine

Advanced Materials, Journal Year: 2018, Volume and Issue: 30(17)

Published: Feb. 16, 2018

Abstract Stem‐cell‐based therapies have the potential to provide novel solutions for treatment of a variety diseases, but main obstacles such lie in uncontrolled differentiation and functional engraftment implanted tissues. The physicochemical microenvironment controls self‐renewal stem cells, key step mimicking cell is construct more physiologically relevant 3D culture system. Material‐based assemblies cells facilitate cellular interactions that promote morphogenesis tissue organization similar manner which occurs during embryogenesis. Both natural artificial materials can be used create scaffolds, synthetic organic inorganic porous are two kinds materials. Nanotechnology provides new opportunities design advanced with special properties transplantation. Herein, advances advantages scaffold materials, especially respect stem‐cell‐based therapies, first outlined. Second, biology reviewed. Third, progress basic principles developing clinical applications engineering regenerative medicine

Language: Английский

---

Alginate/laponite hydrogel microspheres co-encapsulating dental pulp stem cells and VEGF for endodontic regeneration

Acta Biomaterialia, Journal Year: 2020, Volume and Issue: 113, P. 305 - 316

Published: July 12, 2020

Language: Английский

---

Recent Advances on Cell Culture Platforms for In Vitro Drug Screening and Cell Therapies: From Conventional to Microfluidic Strategies

Advanced Healthcare Materials, Journal Year: 2023, Volume and Issue: 12(18)

Published: March 11, 2023

Abstract The clinical translations of drugs and nanomedicines depend on coherent pharmaceutical research based biologically accurate screening approaches. Since establishing the 2D in vitro cell culture method, scientific community has improved cell‐based drug assays models. Those advances result more informative biochemical development 3D multicellular models to describe biological complexity better enhance simulation vivo microenvironment. Despite overall dominance conventional macroscopic methods, they present physicochemical operational challenges that impair scale‐up by not allowing a high parallelization, multidrug combination, high‐throughput screening. Their combination complementarity with microfluidic platforms enable microfluidics‐based unequivocal advantages therapies. Thus, this review presents an updated consolidated view miniaturization's physical, chemical, considerations scenario. It clarifies field using gradient‐based microfluidics, droplet‐based printed‐based digital‐based SlipChip, paper‐based microfluidics. Finally, it comparative analysis performance methods life achieve increased precision process.

Language: Английский

---