Age-related alveolar bone maladaptation in adult orthodontics: finding new ways out

International Journal of Oral Science, Journal Year: 2024, Volume and Issue: 16(1)

Published: Aug. 1, 2024

Abstract Compared with teenage patients, adult patients generally show a slower rate of tooth movement and more pronounced alveolar bone loss during orthodontic treatment, indicating the maladaptation homeostasis under force. However, this phenomenon is not well-elucidated to date, leading increased treatment difficulties unsatisfactory outcomes in orthodontics. Aiming provide comprehensive knowledge further inspire insightful understanding towards issue, review summarizes current evidence underlying mechanisms. The age-related abatements mechanosensing mechanotransduction cells periodontal tissue may contribute retarded unbalanced metabolism, thus hindering reconstruction treatment. To end, surgery, physical chemical cues are being developed reactivate or rejuvenate aging periodontium restore dynamic equilibrium orthodontic-mediated metabolism. We anticipate that will present general overview role plays metabolism shed new light on prospective ways out impasse.

Language: Английский

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Activated neutrophil-derived exosomes contribute to blood–brain barrier damage and hemorrhagic transformation after cerebral ischemia/reperfusion

Brain Research, Journal Year: 2023, Volume and Issue: 1810, P. 148374 - 148374

Published: April 26, 2023

Language: Английский

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Targeting Non-Coding RNA for CNS Injuries: Regulation of Blood-Brain Barrier Functions

Neurochemical Research, Journal Year: 2023, Volume and Issue: 48(7), P. 1997 - 2016

Published: Feb. 14, 2023

Language: Английский

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Advances in the application of extracellular vesicles derived from three-dimensional culture of stem cells

Journal of Nanobiotechnology, Journal Year: 2024, Volume and Issue: 22(1)

Published: May 1, 2024

Abstract Stem cells (SCs) have been used therapeutically for decades, yet their applications are limited by factors such as the risk of immune rejection and potential tumorigenicity. Extracellular vesicles (EVs), a key paracrine component stem cell potency, overcome drawbacks cell-free therapeutic agent play an important role in treating various diseases. However, EVs derived from two-dimensional (2D) planar culture SCs low yield face challenges large-scale production, which hinders clinical translation EVs. Three-dimensional (3D) culture, given its ability to more realistically simulate vivo environment, can not only expand large quantities, but also improve activity EVs, changing content improving effects. In this review, we briefly describe advantages EV-related applications, provide overview 3D finally focus on specific future perspectives different SCs. Graphical

Language: Английский

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Mesenchymal stem cell‐derived extracellular vesicles: Recent therapeutics and targeted drug delivery advances

Journal of Extracellular Biology, Journal Year: 2024, Volume and Issue: 3(5)

Published: May 1, 2024

Abstract The targeted drug delivery field is rapidly advancing, focusing on developing biocompatible nanoparticles that meet rigorous criteria of non‐toxicity, biocompatibility, and efficient release encapsulated molecules. Conventional synthetic (SNPs) face complications such as elevated immune responses, complex synthesis methods, toxicity, which restrict their utility in therapeutics delivery. Extracellular vesicles (EVs) have emerged promising substitutes for SNPs, leveraging ability to cross biological barriers, reduced natural origin. Notably, mesenchymal stem cell‐derived EVs (MSC‐EVs) garnered much curiosity due potential Studies suggest MSC‐EVs, the central paracrine contributors MSCs, replicate therapeutic effects MSCs. This review explores characteristics emphasizing various diseases, including CRISPR/Cas9 gene editing. It also delves into obstacles challenges MSC‐EVs clinical applications provides insights strategies overcome limitations biodistribution target

Language: Английский

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Mesenchymal Stromal Cells Act as Biopatches for Blood–Retinal Barrier Preservation to Enhance Functional Recovery after Retinal Ischemia/Reperfusion

Molecular Therapy — Nucleic Acids, Journal Year: 2025, Volume and Issue: 36(1), P. 102445 - 102445

Published: Jan. 2, 2025

Retinal ischemia/reperfusion (I/R) is one of the most common pathologies many vision-threatening diseases and caused by blood-retinal barrier (BRB) breakdown resulting inflammatory infiltration. Targeting BRB promising for retinal I/R treatment. Mesenchymal stromal cells (MSCs) are emerging as novel therapeutic strategies. Although intravitreal injection targets retina, restricted number injected still requires precise biodistribution MSCs near injury site. Here, we found that led to breakdown, which induced protein cell leakage from circulation. death diminished visual function were subsequently detected. Moreover, expression chemokine CCL5 increased after I/R, colocalized with BRB. We then overexpressed CCR5 in human pluripotent stem cell-derived (iMSCs). In vivo, intravitreal-injected iMSCCCR5 preferentially migrated directly integrated into BRB, preferably restored integrity eventually promoted recovery I/R. summary, our work suggested iMSCs act biopatches preservation iMSC-based therapy a approach related

Language: Английский

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Synthesis and bioactivity evaluation of glycosylated resveratrol derivatives as antioxidative neuroprotection agents against cerebral Ischemia-Reperfusion injury

Bioorganic Chemistry, Journal Year: 2024, Volume and Issue: 153, P. 107791 - 107791

Published: Sept. 3, 2024

Language: Английский

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MiR-125b-5p ameliorates ox-LDL-induced vascular endothelial cell dysfunction by negatively regulating TNFSF4/TLR4/NF-κB signaling

BMC Biotechnology, Journal Year: 2025, Volume and Issue: 25(1)

Published: Jan. 24, 2025

Oxidized low-density lipoprotein (ox-LDL)-induced endothelial cell dysfunction plays a crucial role in the progression of atherosclerosis (AS). Although miR-125b-5p is known to be involved cardiovascular and cerebrovascular disorders, its function ox-LDL-induced injury still not well understood. An vitro AS model was established by exposing human umbilical vein cells (HUVECs) 100 µg/mL ox-LDL for 24 h. A series functional assays, including CCK-8 assay, flow cytometry, MDA SOD kits, capillary-like network formation assay ELISA were performed vitro. TNFSF4/TLR4/NF-κB pathway-related protein expressions measured Western blot. Molecular mechanisms elucidated through quantitative real-time PCR, western blot analysis, luciferase reporter assays. Our investigation revealed that exposure led downregulation miR-125b-5p, while upregulating expression tumor necrosis factor (ligand) superfamily, member 4 (TNFSF4), TLR4, p-p65 p-IkBa HUVECs dose-dependent manner. We confirmed TNFSF4 as direct target miR-125b-5p. Ox-LDL decreased viability angiogenic capacity, along with increased apoptosis, inflammation, oxidative stress HUVECs. These effects reversed overexpressing or knocking down TNFSF4. Overexpression significantly brought about exposed ox-LDL. Moreover, inactivated TLR4/NF-κB signaling pathway negatively regulating In summary, our findings demonstrate possessed an anti-inflammatory anti-apoptosis against HUVEC signaling, indicating may have important therapeutic AS.

Language: Английский

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miRNA in blood-brain barrier repair: role of extracellular vesicles in stroke recovery

Frontiers in Cellular Neuroscience, Journal Year: 2025, Volume and Issue: 19

Published: Feb. 7, 2025

Ischemic stroke is a leading cause of mortality and long-term disability globally. One its aspects the breakdown blood-brain barrier (BBB). The disruption BBB's integrity during exacerbates neurological damage hampers therapeutic intervention. Recent advances in regenerative medicine suggest that mesenchymal stem cells (MSCs) derived extracellular vesicles (EVs) show promise for restoring BBB integrity. This review explores potential MSC-derived EVs mediating neuroprotective reparative effects on after ischemic stroke. We highlight molecular cargo EVs, including miRNAs, their role enhancing angiogenesis, promoting neural repair, mitigating apoptosis. Furthermore, we discuss challenges associated with clinical translation EV therapies possibilities further EVs' innate protective qualities. Our findings underscore need research to optimize establish efficacy safety settings.

Language: Английский

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MicroRNAs Associated with Parenchymal Hematoma After Endovascular Mechanical Reperfusion for Acute Ischemic Stroke in Rats

Biomedicines, Journal Year: 2025, Volume and Issue: 13(2), P. 449 - 449

Published: Feb. 12, 2025

Background/Objectives: Hemorrhagic transformation after endovascular thrombectomy predicts poor outcomes in acute ischemic stroke with large-vessel occlusion. The roles of microRNAs (miRNAs) the pathogenesis parenchymal hematoma (PH) still remain unclear. This study aimed to investigate miRNA and mRNA regulatory network associated PH mechanical reperfusion an animal model oxygen-glucose deprivation/reoxygenation (OGD/R) model. Methods: Twenty-five miRNAs were assessed a reperfusion-induced hemorrhage rats under hyperglycemic conditions receiving 5 h middle cerebral artery differentially expressed neuron, astrocyte, microglia, brain microvascular endothelial cell (BMEC), pericyte OGD/R. predicted target genes further miRNA-mRNA was established. Results: Thirteen down-regulated (miRNA-29a-5p, miRNA-29c-3p, miRNA-126a-5p, miRNA-132-3p, miRNA-136-3p, miRNA-142-3p, miRNA-153-5p, miRNA-218a-5p, miRNA-219a-2-3p, miRNA-369-5p, miRNA-376a-5p, miRNA-376b-5p, miRNA-383-5p) one up-regulated (miRNA-195-3p) found rat peri-infarct reperfusion. Of these 14 PH-related miRNAs, 10 significantly at least two five BMEC, models OGD/R, consistent results. Thirty-one hub Forty-nine axes revealed, they related mechanisms inflammation, immunity, oxidative stress, apoptosis. Conclusions: Fourteen OGD/R models. Simultaneously several cells neurovascular unit may serve as valuable targets for stroke.

Language: Английский

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