Application of an in vitro neuroinflammation model to evaluate the efficacy of magnesium-lithium alloys DOI Creative Commons
Krathika Bhat, Heike Helmholz, Regine Willumeit–Römer

et al.

Frontiers in Cellular Neuroscience, Journal Year: 2024, Volume and Issue: 18

Published: Oct. 29, 2024

Mg-Li alloys can be promising candidates as bioresorbable Li-releasing implants for bipolar disorder and other neurodegenerative disorders. In order to compare the therapeutic efficacy of conventional Li salts delivered through alloy extracts, we tested an in vitro model based on neuroinflammation hypothesis mood disorders (peripheral inflammation inducing neuroinflammation) wherein, a coculture microglia astrocytes was treated with conditioned medium from pro-inflammatory macrophages. Two alloys, Mg-1.6Li Mg-9.5Li, were form material extracts well-known outcomes treatment such GSK3β phosphorylation (indirect flow cytometry) influence inflammation-related gene expression (qPCR) compared against salts. This is first study demonstrating that increase glial cells presence excess Mg. Furthermore, more effective than downregulating IL6 upregulating neurotrophin GDNF . Mg had no antagonistic effects toward Li-driven downregulation astrogliosis markers. Overall, results provide evidence support further studies employing neurological applications.

Language: Английский

Effect of heat treatment on microstructure evolution, strengthening-toughening and corrosion behavior of high-strength LA103Z Mg-Li alloy fabricated by wire-arc additive manufacturing DOI
Dengke Liu, Xuewen Zong,

Pengsheng Xue

et al.

Journal of Material Science and Technology, Journal Year: 2025, Volume and Issue: unknown

Published: May 1, 2025

Language: Английский

Citations

0
Insight into the self-healing performance of dicalcium phosphate dihydrate coating on magnesium wires: its damage and anti-corrosive behavior under deformation DOI
Yue Zhang,

Tingjie Nie,

Dongfang Chen

et al.

Corrosion Science, Journal Year: 2025, Volume and Issue: unknown, P. 113051 - 113051

Published: May 1, 2025

Language: Английский

Citations

0
Multifunctional hydrogel dressing promotes wound healing by reprogramming the infection-related wound microenvironment DOI

Yaqi Xi,

Shuo Zhang, Meng-Ru Wu

et al.

Journal of Materials Science, Journal Year: 2024, Volume and Issue: 59(35), P. 16660 - 16677

Published: Sept. 1, 2024

Language: Английский

Citations

2
Influence of Magnesium Degradation on Schwannoma Cell Responses to Nerve Injury Using an In Vitro Injury Model DOI Creative Commons
Krathika Bhat, Lisa Hanke, Heike Helmholz

et al.

Journal of Functional Biomaterials, Journal Year: 2024, Volume and Issue: 15(4), P. 88 - 88

Published: March 31, 2024

Nerve guidance conduits for peripheral nerve injuries can be improved using bioactive materials such as magnesium (Mg) and its alloys, which could provide both structural trophic support. Therefore, we investigated whether exposure to Mg Mg-1.6wt%Li thin films (Mg/Mg-1.6Li) would alter acute Schwann cell responses injury. Using the RT4-D6P2T Schwannoma line (SCs), tested extracts from freeze-killed cells (FKC) nerves (FKN) in vitro injury stimulants. Both FKC FKN induced SC release of macrophage chemoattractant protein 1 (MCP-1), a marker repair phenotype after Next, FKC-stimulated exposed Mg/Mg-1.6Li reduced MCP-1 by 30%, suggesting that these have anti-inflammatory effects. Exposing FKC-treated gene expression growth factor (NGF), glial line-derived neurotrophic (GDNF), myelin zero (MPZ), but not p75 neurotrophin receptor. In absence FKC, treatment increased NGF, p75, MPZ, beneficial regeneration. Thus, presence differentially SCs, depending on microenvironment. These results demonstrate applicability this model, has wide-ranging effects phenotype.

Language: Английский

Citations

1
Investigation of in-situ ion release and surface film formation of hcp Mg-Li thin films DOI Creative Commons
Lisa Hanke,

Lukas Kalchgruber,

Ulrike Westernströer

et al.

Corrosion Science, Journal Year: 2024, Volume and Issue: 238, P. 112361 - 112361

Published: Aug. 13, 2024

Language: Английский

Citations

1
Application of an in vitro neuroinflammation model to evaluate the efficacy of magnesium-lithium alloys DOI Creative Commons
Krathika Bhat, Heike Helmholz, Regine Willumeit–Römer

et al.

Frontiers in Cellular Neuroscience, Journal Year: 2024, Volume and Issue: 18

Published: Oct. 29, 2024

Mg-Li alloys can be promising candidates as bioresorbable Li-releasing implants for bipolar disorder and other neurodegenerative disorders. In order to compare the therapeutic efficacy of conventional Li salts delivered through alloy extracts, we tested an in vitro model based on neuroinflammation hypothesis mood disorders (peripheral inflammation inducing neuroinflammation) wherein, a coculture microglia astrocytes was treated with conditioned medium from pro-inflammatory macrophages. Two alloys, Mg-1.6Li Mg-9.5Li, were form material extracts well-known outcomes treatment such GSK3β phosphorylation (indirect flow cytometry) influence inflammation-related gene expression (qPCR) compared against salts. This is first study demonstrating that increase glial cells presence excess Mg. Furthermore, more effective than downregulating IL6 upregulating neurotrophin GDNF . Mg had no antagonistic effects toward Li-driven downregulation astrogliosis markers. Overall, results provide evidence support further studies employing neurological applications.

Language: Английский

Citations

0