Microfluidics and Nanofluidics, Journal Year: 2023, Volume and Issue: 27(2)
Published: Jan. 10, 2023
Language: Английский
Advanced Materials, Journal Year: 2022, Volume and Issue: 34(13)
Published: Jan. 22, 2022
Lung-on-a-chip models hold great promise for disease modeling and drug screening. Herein, inspired by the iridescence phenomenon of soap bubbles, a novel biomimetic 3D microphysiological lung-on-a-chip system with breathing visualization is presented. The system, an array pulmonary alveoli at physiological scale, constructed coated structural color materials. Cyclic deformation induced regular airflow, resembling expansion contraction during rhythmic breathing. As accompanied corresponding synchronous shifts in color, offers self-reporting cell mechanics enables real-time monitoring cultivation process. Using this dynamic relationships between atlas symptoms, showing essential role mechanical stretching phenotypes idiopathic fibrosis, are investigated. These features make human lung ideal biological study, monitoring, discovery.
Language: Английский
Citations
88
ACS Biomaterials Science & Engineering, Journal Year: 2023, Volume and Issue: 9(5), P. 2806 - 2815
Published: April 20, 2023
There is an urgent need for physiologically relevant and customizable biochip models of human lung tissue to provide a niche disease modeling drug efficacy. Although various lung-on-a-chips have been developed, the conventional fabrication method has limited in reconstituting very thin multilayered architecture spatial arrangements multiple cell types microfluidic device. To overcome these limitations, we developed alveolar lung-on-a-chip model, effectively integrated with inkjet-printed, micron-thick, three-layered tissue. After bioprinting tissues inside four culture inserts layer-by-layer, are implanted into that supplies flow medium. This modular implantation procedure enables formation facilitate 3D-structured inkjet-bioprinted under perfusion at air–liquid interface. The bioprinted cultured on chip maintained their structure three layers tens micrometers achieved tight junction epithelial layer, critical properties barrier. upregulation genes involved essential functions alveoli was also confirmed our model. Our insert-mountable organ-on-a-chip versatile platform can be applied organ by implanting replacing inserts. It amenable mass production development customized through convergence technology.
Language: Английский
Citations
34
Advanced Healthcare Materials, Journal Year: 2023, Volume and Issue: 13(21)
Published: July 22, 2023
Organoids and cells in organ-on-chip platforms replicate higher-level anatomical, physiological, or pathological states of tissues organs. These technologies are widely regarded by academia, the pharmacological industry regulators as key biomedical developments. To map advances this emerging field, a literature analysis 16,000 article metadata based on quality-controlled text-mining algorithm is performed. The covers titles, keywords, abstracts categorized academic publications preprint databases published after 2010. identifies tracks 149 107 organs organ substructures modeled organoids organ-on-chip, respectively, stem cell sources, well 130 diseases, 16 groups organisms other than human mouse which organoid/organ-on-chip technology applied. illustrates changing diversity focus research captures its geographical distribution. downloadable dataset provided robust framework for researchers to interrogate with their own questions.
Language: Английский
Citations
29
Frontiers in Pharmacology, Journal Year: 2023, Volume and Issue: 14
Published: March 6, 2023
Prolonged exposure to environmental respirable toxicants can lead the development and worsening of severe respiratory diseases such as asthma, chronic obstructive pulmonary disease (COPD) fibrosis. The limited number FDA-approved inhaled drugs for these serious lung conditions has led a shift from in vivo towards use alternative vitro human-relevant models better predict toxicity particles preclinical research. While there are several inhalation upper airways, fragile dynamic nature alveolar microenvironment reproducible distal lung. Here, we present mechanistic approach using new generation systems, Cloud α AX12. This novel tool consists cloud-based chamber (VITROCELL) that integrates breathing AX Lung-on-chip system (AlveoliX). ultrathin porous membrane AX12 plate was used create complex multicellular model enables key physiological culture conditions: air-liquid interface (ALI) three-dimensional cyclic stretch (CS). Human-relevant cellular were established a) alveolar-capillary primary cell-derived immortalized epithelial cells ( iAECs), macrophages (THP-1) endothelial (HLMVEC) cells, b) upper-airways Calu3 cells. Primary human hAEpCs) validate results obtained cell lines. To mimic relevant aerosol exposures with AX12, three different using: titanium dioxide (TiO2) zinc oxide nanoparticles polyhexamethylene guanidine toxic chemical c) an anti-inflammatory corticosteroid, fluticasone propionate (FL). Our suggest important synergistic effect on air-blood barrier sensitivity, cytotoxicity inflammation, when combined. best our knowledge, this is first time been described lung-on-chip technology. thus represents state-of-the-art pre-clinical study risks, drug safety efficacy.
Language: Английский
Citations
24
Advanced Materials, Journal Year: 2023, Volume and Issue: 35(13)
Published: Feb. 7, 2023
As post-COVID complications, chronic respiratory diseases are one of the foremost causes mortality. The quest for a cure this recent global challenge underlines that lack predictive in vitro lung models is main bottlenecks pulmonary preclinical drug development. Despite rigorous efforts to develop biomimetic models, current cutting-edge represent compromise numerous technological and biological aspects. Most advanced still "proof-of-concept" phase with low clinical translation findings. On other hand, advances cellular molecular studies mainly based on relatively simple unrealistic models. Herein, challenges potential strategies toward not only bioinspired but truly discussed.
Language: Английский
Citations
19
Journal of Tissue Engineering, Journal Year: 2024, Volume and Issue: 15
Published: Jan. 1, 2024
Amidst the recent coronavirus disease 2019 (COVID-19) pandemic, respiratory system research has made remarkable progress, particularly focusing on infectious diseases. Lung organoid, a miniaturized structure recapitulating lung tissue, gained global attention because of its advantages over other conventional models such as two-dimensional (2D) cell and animal models. Nevertheless, organoids still face limitations concerning heterogeneity, complexity, maturity compared to native tissue. To address these limitations, researchers have employed co-culture methods with various types including endothelial cells, mesenchymal immune incorporated bioengineering platforms air-liquid interfaces, microfluidic chips, functional hydrogels. These advancements facilitated applications studies pulmonary diseases, providing insights into mechanisms potential treatments. This review introduces progress in production organoids, strategies for improving maturity, functionality, complexity their application modeling, infection fibrosis.
Language: Английский
Citations
7
Frontiers in Bioengineering and Biotechnology, Journal Year: 2022, Volume and Issue: 10
Published: Feb. 18, 2022
The gas exchange units of the lung, alveoli, are mechanically active and undergo cyclic deformation during breathing. epithelial cells that line alveoli contribute to lung function by reducing surface tension via surfactant secretion, which is highly influenced breathing-associated mechanical cues. These spatially heterogeneous cues have been linked several physiological pathophysiological states. Here, we describe development a microfluidically assisted cell culture model incorporates stretching mimic alveolar respiratory motions. Employing this device, examined effects biomechanics (associated with breathing-like movements) strain heterogeneity on functions. Furthermore, assessed potential application platform altered matrix compliance associated pathogenesis ventilator-induced injury. Lung microphysiological platforms incorporating human dynamic could serve as an important tool delineate role micromechanics in pathological outcomes lung.
Language: Английский
Citations
24
European Journal of Pharmaceutical Sciences, Journal Year: 2022, Volume and Issue: 180, P. 106337 - 106337
Published: Nov. 21, 2022
Human lung tissue models range from simple monolayer cultures to more advanced three-dimensional co-cultures. Each model system can address the interactions of different types aerosols and choice mode aerosol exposure depends on relevant scenario, such as adverse outcomes endpoints interest. This review focuses functional, well structural, aspects upper airway distal alveolar compartments this information is for design a how properties determine interfacial with respiratory wall. The most important are summarized focus (i) appropriate scaffold, (ii) selection cell healthy diseased models, (iii) use culture condition assembly, (iv) methods, (v) verification process. Finally, remaining challenges future directions in field discussed.
Language: Английский
Citations
24
European Journal of Pharmacology, Journal Year: 2022, Volume and Issue: 919, P. 174821 - 174821
Published: Feb. 11, 2022
Language: Английский
Citations
23
Frontiers in Immunology, Journal Year: 2023, Volume and Issue: 14
Published: Feb. 23, 2023
Asthma is a chronic lung disease involving airway inflammation and fibrosis. Fibroblasts are the main effector cells important for tissue production which becomes abnormal in asthmatics one of contributors to Although fibroblasts were traditionally viewed solely as structural cells, they have been discovered be highly active, involved inflammatory fibrotic processes asthma. In line with this, using 2D 3D vitro co-culture models, complex interaction between various immune pathogenesis asthma recently uncovered. Hence, this review, we provide first-ever summary studies that used models assess nature aberrant cell-fibroblast interactions their contributions mechanisms pathogenesis.
Language: Английский
Citations
15