A bionic controllable strain membrane for cell stretching at air–liquid interface inspired by papercutting

International Journal of Extreme Manufacturing, Journal Year: 2023, Volume and Issue: 5(4), P. 045502 - 045502

Published: Aug. 11, 2023

Abstract Lung diseases associated with alveoli, such as acute respiratory distress syndrome, have posed a long-term threat to human health. However, an in vitro model capable of simulating different deformations the alveoli and suitable material for mimicking basement membrane are currently lacking. Here, we present innovative biomimetic controllable strain (BCSM) at air–liquid interface (ALI) reconstruct alveolar respiration. The BCSM consists high-precision three-dimensional printing melt-electrowritten polycaprolactone (PCL) mesh, coated hydrogel substrate—to simulate important functions (such stiffness, porosity, wettability, ALI) microenvironments, seeded pulmonary epithelial cells vascular endothelial on either side, respectively. Inspired by papercutting, was fabricated plane while it operated three dimensions. A series topological structure designed control various local-area strain, varied deformation. Lopinavir/ritonavir could reduce Lamin expression under over-stretch condition, which might be effective preventing ventilator-induced lung injury. lung-unit has broader application prospects alveoli-related research future, drug toxicology metabolism.

Language: Английский

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Investigation of the role of the autophagic protein LC3B in the regulation of human airway epithelium cell differentiation in COPD using a biomimetic model

Materials Today Bio, Journal Year: 2021, Volume and Issue: 13, P. 100182 - 100182

Published: Dec. 8, 2021

Chronic obstructive pulmonary disease (COPD) is one of the most lethal chronic worldwide; however, establishment reliable in vitro models for exploring biological mechanisms COPD remains challenging. Here, we determined differences expression and characteristics autophagic protein LC3B normal human small airway epithelial cells found that nucleus obviously accumulated LC3B. We next established 3D tissues with distinct by regulating microenvironment, extracellular matrix, air-liquid interface culture methods. Using this biomimetic model, affects differentiation into basal, secretory, mucous, ciliated cells. Moreover, although chloroquine ivermectin effectively inhibited nucleus, specifically maintained performance cytoplasm, thereby contributing to subsequent improvement beating functions cilia, whereas only facilitated goblet demonstrated mechanism factor ciliary function Our innovative model can be used further analyze physiological environment.

Language: Английский

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Transwell‐Based Microfluidic Platform for High‐Resolution Imaging of Airway Tissues

Advanced Materials Technologies, Journal Year: 2024, Volume and Issue: 9(20)

Published: July 10, 2024

Abstract Transwell‐based airway models have become increasingly important in studying the effects of respiratory diseases and drug treatment at air–liquid interface lung epithelial barrier. However, underlying mechanisms tissue cell level often remain unclear, as transwell inserts feature limited live‐cell imaging compatibility. Here, a novel microfluidic platform is reported for cultivation transwell‐based tissues providing possibility to alternate between liquid–liquid interfaces. While recapitulates physiological conditions model, enables live high spatiotemporal resolution. The plastics‐based insertion recuperation inserts, which allows analysis under standardized well plate conditions. device used monitor infections Pseudomonas aeruginosa human stem‐cell‐derived bronchial tissue. progression P. infection real‐time resolution continuously imaged, provides insights into bacterial spreading invasion on apical surface, breaching destruction over time. culture system powerful tool visualize elucidate key processes developing facilitate testing development.

Language: Английский

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Ventilation‐induced epithelial injury drives biological onset of lung trauma in vitro and is mitigated with prophylactic anti‐inflammatory therapeutics

Bioengineering & Translational Medicine, Journal Year: 2021, Volume and Issue: 7(2)

Published: Nov. 17, 2021

Mortality rates among patients suffering from acute respiratory failure remain perplexingly high despite the maintenance of blood oxygen homeostasis during ventilatory support. The biotrauma hypothesis advocates that mechanical forces invasive ventilation trigger immunological mediators spread systemically. Yet, how these elicit an immune response remains unclear. Here, a biomimetic in vitro three-dimensional (3D) upper airways model allows to recapitulate lung injury and responses induced neonates. Under such support, flow-induced stresses injure bronchial epithelium intubated directly modulate epithelial cell inflammatory cytokine secretion associated with pulmonary injury. Fluorescence microscopy biochemical analyses reveal site-specific susceptibility erosion jet-flow impaction are linked increases apoptosis modulated secretions cytokines IL-6, -8, -10. In effort mitigate onset biotrauma, prophylactic pharmacological treatment Montelukast, leukotriene receptor antagonist, reduces pro-inflammatory signaling model. This 3D airway platform points previously overlooked origin showcases translational opportunities preclinical research toward protective therapies improved protocols for patient care.

Language: Английский

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Development of microfluidic platform that enables ‘on-chip’ imaging of cells exposed to shear stress and cyclic stretch

Microfluidics and Nanofluidics, Journal Year: 2023, Volume and Issue: 27(2)

Published: Jan. 10, 2023

Language: Английский

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