Bacterial extracellular vesicles-based therapeutic strategies for bone and soft tissue tumors therapy

Theranostics, Journal Year: 2022, Volume and Issue: 12(15), P. 6576 - 6594

Published: Jan. 1, 2022

Bone and soft tissue tumors are complex mesenchymal neoplasms that seriously endanger human health. Over the past decade, relationship between microorganisms health diseases is getting more attention. The extracellular vesicles derived from bacteria have been shown to regulate bacterial-host cell communication by transferring their contents, including nucleic acids, proteins, metabolites, lipopolysaccharides, peptidoglycans. Bacteria (BEVs) promising lipid-bilayer nanocarriers for treatment of many due low toxicity, drug loading capacity, ease modification industrialization. Specially, BEVs-based cancer therapy has attracted much attention because ability effectively stimulate immune responses. In this review, we provide an overview biogenesis, composition, isolation, classification, internalization BEVs. We then comprehensively summarize sources BEVs in BEVs-related strategies. further highlight great potential bone tumors. Finally, conclude major advantages challenges therapy. believe comprehensive understanding field will generate innovative solutions achieve clinical applications.

Language: Английский

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Engineered bacterial extracellular vesicles for osteoporosis therapy

Chemical Engineering Journal, Journal Year: 2022, Volume and Issue: 450, P. 138309 - 138309

Published: July 30, 2022

Osteoporosis (OP) is a systemic bone disease characterized by reduction of mass and destruction structure, which can easily lead to fragility fractures. Over the past decade, an expanding body study suggests that gut microbiota (GM) plays important role in homeostasis pathogenesis OP. The communication mechanism between GM OP has received increasing attention. Bacterial extracellular vesicles (BEVs) are irreproducible phospholipid bilayer nanocarriers with sizes ranging from 20 400 nm, mediate delivering various bioactive molecules. BEVs have emerged as promising platform for treatment due their nanosized structures, low toxicity, drug loading capacity, good biocompatibility, ease modification industrialization. Here, we summarize overview BEVs, including biogenies, classes, compositions, internalization, isolation BEVs. We then focus on applications natural Subsequently, present two major engineering strategies, parental strain after isolation, modifying treatment. Finally, provide outlook challenges future prospects hope comprehensive understanding engineered will new solutions

Language: Английский

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Biomimetic cell-derived nanocarriers in cancer research

Journal of Nanobiotechnology, Journal Year: 2022, Volume and Issue: 20(1)

Published: Dec. 22, 2022

Abstract Nanoparticles have now long demonstrated capabilities that make them attractive to use in biology and medicine. Some of them, such as lipid nanoparticles (SARS-CoV-2 vaccines) or metallic (contrast agents) are already approved for their the clinic. However, considering constantly growing body different formulations huge research around nanomaterials number candidates reaching clinical trials being commercialized is minimal. The reasons behind related “synthetic” “foreign” character surface. Typically, aiming develop a function deliver cargo locally, fail by showing strong off-target accumulation generation adverse responses, which connected recognition immune phagocytes primarily. Therefore, rendering negligible numbers developing intended function. While wide range coatings has been applied avoid certain interactions with surrounding milieu, issues remained. Taking advantage natural cell membranes, an approach resembles transfer, cell-derived surfaces risen alternative artificial encapsulation methods. Biomimetic technologies based on isolated components provide autologous properties nanoparticle encapsulated, thus, improving therapeutic behavior. main goal replicate (bio)-physical functionalities source tissue, not only providing stealthy core but also taking homotypic properties, could prove relevant targeted strategies. Such biomimetic potential overcome approaches specific features identities synthetically. In this review, we insight into challenges nano-biointerfaces drug delivery; applications materials derived from types, focusing unique strengths fabrication novel nanotherapeutics cancer therapy. Graphical

Language: Английский

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Targeting Pyroptosis through Lipopolysaccharide-Triggered Noncanonical Pathway for Safe and Efficient Cancer Immunotherapy

Nano Letters, Journal Year: 2023, Volume and Issue: 23(18), P. 8725 - 8733

Published: Sept. 11, 2023

Inducing pyroptosis in cancer cells holds great potential immunotherapy. Lipopolysaccharide (LPS)-sensing noncanonical pathways are an important mechanism of to eliminate damaged cells, which has not yet been explored for Here, we utilize bacterial outer membrane vesicles (OMVs) as a natural LPS carrier trigger pathway To address the concern systemic toxicity, molecule engineered OMVs were designed by equipping DNA aptamers on (Apt-OMVs). In addition improving capacity target tumors, Apt-OMVs also took advantage spherical nucleic acid structure shield against nonspecific immune recognition and evade immunogenicity. The selective enhanced tumor immunogenicity, only promoting infiltration effector T but reducing amount immunosuppressive regulatory remarkably suppressed growth. This work reports first inducer pathway, offering inspiration safe efficient pyroptosis-mediated

Language: Английский

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Cloaking Mesoporous Polydopamine with Bacterial Membrane Vesicles to Amplify Local and Systemic Antitumor Immunity

ACS Nano, Journal Year: 2023, Volume and Issue: 17(8), P. 7733 - 7749

Published: April 10, 2023

As adjuvants or antigens, bacterial membranes have been widely used in recent antibacterial and antitumor research, but they are often injected multiple times to achieve therapeutic outcomes, with limitations biosafety clinical application. Herein, we leverage the biocompatibility immune activation capacity of Salmonella strain VNP20009 produce double-layered membrane vesicles (DMVs) for enhanced systemic safety immunity. Considering photothermal effect polydopamine upon irradiation, VNP20009-derived DMVs prepared coat surface mesoporous (MPD) nanoparticles, leading potential synergies between therapy mediated by MPD immunotherapy magnified DMVs. The single dose MPD@DMV can passively target tumors activate system upregulated T cell infiltration secretion levels pro-inflammatory factors as well related cytokines. All these promoted responses result malignant melanoma tumor regression extended survival time on local distant tumor-bearing mouse models. Importantly, further explore advantages intravenous injection agent compared its intratumoral injection, former demonstrates better long-term effects animal bodies. Overall, this formulation design brings broader prospects autologous vaccine adjuvant cancer therapy.

Language: Английский

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“Bioinspired” Membrane-Coated Nanosystems in Cancer Theranostics: A Comprehensive Review

Pharmaceutics, Journal Year: 2023, Volume and Issue: 15(6), P. 1677 - 1677

Published: June 8, 2023

Achieving precise cancer theranostics necessitates the rational design of smart nanosystems that ensure high biological safety and minimize non-specific interactions with normal tissues. In this regard, “bioinspired” membrane-coated have emerged as a promising approach, providing versatile platform for development next-generation nanosystems. This review article presents an in-depth investigation into potential these targeted theranostics, encompassing key aspects such cell membrane sources, isolation techniques, nanoparticle core selection, approaches coating cores membrane, characterization methods. Moreover, underscores strategies employed to enhance multi-functionality nanosystems, including lipid insertion, hybridization, metabolic engineering, genetic modification. Additionally, applications bioinspired in diagnosis therapeutics are discussed, along recent advances field. Through comprehensive exploration provides valuable insights their theranostics.

Language: Английский

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Genetically engineered cellular nanoparticles for biomedical applications

Biomaterials, Journal Year: 2023, Volume and Issue: 296, P. 122065 - 122065

Published: Feb. 20, 2023

Language: Английский

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Bacterial membrane vesicles in the pathogenesis and treatment of inflammatory bowel disease

Gut Microbes, Journal Year: 2024, Volume and Issue: 16(1)

Published: April 26, 2024

Inflammatory bowel disease (IBD) is a chronic and debilitating condition of relapsing remitting inflammation in the gastrointestinal tract. Conventional therapeutic approaches for IBD have shown limited efficacy detrimental side effects, leading to quest novel effective treatment options disease. Bacterial membrane vesicles (MVs) are nanosized lipid particles secreted by lysis or blebbing processes from both Gram-negative Gram-positive bacteria. These vesicles, known carry bioactive components, facsimiles parent bacterium been implicated onset progression, as well amelioration IBD. This review discusses overview MVs their impact pathogenesis, diagnosis, We further discuss technical challenges facing this research area possible questions addressing these challenges. summarize recent advances diverse relationship between MVs, application knowledge viable potent strategy

Language: Английский

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Cell Membrane‐Coated Nanoparticles: A New Frontier in Immunomodulation

Advanced NanoBiomed Research, Journal Year: 2024, Volume and Issue: 4(8)

Published: May 31, 2024

Immune dysregulation is a pivotal factor in the onset and progression of various diseases. In cancer, immune system's inability to discern eliminate abnormal cells leads uncontrolled tumor growth. When faced with resilient pathogens or harmful toxins, system encounters challenges clearance neutralization. Achieving delicate balance pro‐inflammatory anti‐inflammatory signals essential managing range disorders Like other biomedical research domains, nanotechnology has provided innovative approaches for rebalancing host immunity. Among plethora nanotechnology‐based interventions, concept cell membrane‐coated nanoparticles holds significant potential immunomodulatory applications owing their biomimetic properties that allow precise interaction compromised system. This review thoroughly examines novel nanosystems modulation. The exploration covers crucial elements, including origins characteristics membranes, methods employed procurement coating, physicochemical/biological characterization techniques, enhancement therapeutic efficacy via functionalization. Subsequently, case studies‐based analysis utilizing these bioinspired tackling different conditions caused by disturbance been comprehensively discussed.

Language: Английский

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Consistency in bacterial extracellular vesicle production: key to their application in human health

Extracellular Vesicles and Circulating Nucleic Acids, Journal Year: 2025, Volume and Issue: 6(1), P. 1 - 20

Published: Jan. 17, 2025

Bacterial extracellular vesicles (BEVs) are naturally occurring functional structures that play critical roles in bacterial life processes. These vesicles, commonly known as outer membrane (OMVs), were first found to be released by Gram-negative bacteria; however, it has since been confirmed Gram-positive bacteria also secrete BEVs. As research advances, BEVs increasingly utilized diverse applications, including vaccine development and drug delivery. Nevertheless, the effective employment of these contexts requires acquisition with consistent properties functions through appropriate culture, isolation, purification methods. This review examines advantages disadvantages various techniques alongside heterogeneity they may introduce. We utilize a framework critically analyze barriers their application factors influencing characteristics. Additionally, we constructively propose solutions enhance consistency BEVs, thereby facilitating further application.

Language: Английский

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