Journal of Bioscience and Bioengineering, Journal Year: 2023, Volume and Issue: 136(3), P. 173 - 181
Published: July 22, 2023
Language: Английский
Nature Reviews Clinical Oncology, Journal Year: 2023, Volume and Issue: 20(11), P. 739 - 754
Published: Aug. 16, 2023
Language: Английский
Citations
134
Advanced Drug Delivery Reviews, Journal Year: 2024, Volume and Issue: 211, P. 115359 - 115359
Published: June 12, 2024
Language: Английский
Citations
16
Journal of Hazardous Materials, Journal Year: 2025, Volume and Issue: 487, P. 137194 - 137194
Published: Jan. 11, 2025
Language: Английский
Citations
2
European Journal of Nuclear Medicine and Molecular Imaging, Journal Year: 2025, Volume and Issue: unknown
Published: Jan. 13, 2025
Language: Английский
Citations
2
ACS Applied Materials & Interfaces, Journal Year: 2024, Volume and Issue: 16(43), P. 58405 - 58416
Published: Oct. 16, 2024
Apolipoprotein A1 (Apo-A1) is a well-recognized biomarker in tissues, closely associated with cardiovascular diseases such as atherosclerosis, coronary artery disease, and heart failure. However, existing methods for Apo-A1 determination are limited by costly equipment intricate operational procedures. Given the distinct advantages of electrochemical immunosensors, including affordability high sensitivity, along unique attributes nanobodies (Nbs), enhanced specificity better tissue permeability, we developed an immunosensor detection utilizing Nb technology. In our study, Ce-MOF@AuNPs nanocomposites were synthesized using ultrasonic applied to modify glassy carbon electrode. The Nb6, screened from immunized phage library, was immobilized onto nanocomposite material, establishing robust binding interaction Apo-A1. recorded peak current values demonstrated logarithmic increase corresponding concentrations ranging 1 100,000 pg/mL, limit 36 fg/mL. Additionally, immunosensors selectivity, good stability, reproducibility. Our methodology also effectively utilized serum sample analysis, showing performance clinical assessments. This represents promising tool detection, significant potential advancing disease diagnostics.
Language: Английский
Citations
10
Talanta, Journal Year: 2024, Volume and Issue: 274, P. 126021 - 126021
Published: April 1, 2024
Language: Английский
Citations
9
FEBS Open Bio, Journal Year: 2024, Volume and Issue: 15(2), P. 236 - 253
Published: June 19, 2024
Nanobodies, the smallest functional antibody fragment derived from camelid heavy-chain-only antibodies, have emerged as powerful tools for diverse biomedical applications. In this comprehensive review, we discuss structural characteristics, properties, and computational approaches driving design optimisation of synthetic nanobodies. We explore their unique antigen-binding domains, highlighting critical role complementarity-determining regions in target recognition specificity. This review further underscores advantages nanobodies over conventional antibodies a biosynthesis perspective, including small size, stability, solubility, which make them ideal candidates economical antigen capture diagnostics, therapeutics, biosensing. recent advancements methods nanobody modelling, epitope prediction, affinity maturation, shedding light on intricate mechanisms conformational dynamics. Finally, examine direct example how strategies were implemented improving nanobody-based immunosensor, known Quenchbody. Through combining experimental findings insights, elucidates transformative impact biotechnology research, offering roadmap future applications healthcare diagnostics.
Language: Английский
Citations
6
Exploration, Journal Year: 2023, Volume and Issue: 4(3)
Published: Dec. 15, 2023
The ongoing mutations of the SARS-CoV-2 pose serious challenges to efficacy available antiviral drugs, and new drugs with fantastic are always deserved investigation. Here, a nanobody called IBT-CoV144 is reported, which exhibits broad neutralizing activity against by inducing conformation spike trimer dimers. was isolated from an immunized alpaca using RBD wild-type SARS-CoV-2, it showed strong cross-reactive binding potency diverse variants, including Omicron subvariants. Moreover, prophylactically therapeutically intranasal administration confers protective challenge BA.1 variant in BALB/c mice model. structure analysis complex between (S) protein, conducted Cryo-EM, revealed special known as This formed two trimers, six RBDs "up" state bound VHHs. binds lateral region on S facilitating aggregation proteins. results steric hindrance, disrupts recognition virus ACE2 host cells. discovery will provide valuable insights for development advanced therapeutics design next-generation vaccines.
Language: Английский
Citations
15
Biomedicine & Pharmacotherapy, Journal Year: 2024, Volume and Issue: 176, P. 116900 - 116900
Published: June 10, 2024
Language: Английский
Citations
4
Briefings in Bioinformatics, Journal Year: 2024, Volume and Issue: 25(4)
Published: May 23, 2024
Abstract Deep learning has achieved impressive results in various fields such as computer vision and natural language processing, making it a powerful tool biology. Its applications now encompass cellular image classification, genomic studies drug discovery. While development traditionally focused deep on small molecules, recent innovations have incorporated the discovery of biological particularly antibodies. Researchers devised novel techniques to streamline antibody development, combining vitro silico methods. In particular, computational power expedites lead candidate generation, scaling potential against complex antigens. This survey highlights significant advancements protein design optimization, specifically focusing includes aspects design, folding, antibody–antigen interactions docking affinity maturation.
Language: Английский
Citations
4