Chemical Reviews,
Journal Year:
2024,
Volume and Issue:
124(20), P. 11242 - 11347
Published: Oct. 9, 2024
Biopsy,
including
tissue
and
liquid
biopsy,
offers
comprehensive
real-time
physiological
pathological
information
for
disease
detection,
diagnosis,
monitoring.
Fluorescent
probes
are
frequently
selected
to
obtain
adequate
on
processes
in
a
rapid
minimally
invasive
manner
based
their
advantages
biopsy.
However,
conventional
fluorescent
have
been
found
show
aggregation-caused
quenching
(ACQ)
properties,
impeding
greater
progresses
this
area.
Since
the
discovery
of
aggregation-induced
emission
luminogen
(AIEgen)
promoted
advancements
molecular
bionanomaterials
owing
unique
high
quantum
yield
(QY)
signal-to-noise
ratio
(SNR),
ACS Nano,
Journal Year:
2024,
Volume and Issue:
18(19), P. 12168 - 12186
Published: April 30, 2024
Assessment
of
hypoxia,
nutrients,
metabolite
gradients,
and
other
hallmarks
the
tumor
microenvironment
within
3D
multicellular
spheroid
organoid
models
represents
a
challenging
analytical
task.
Here,
we
report
red/near-infrared
(NIR)
emitting
cell
staining
with
O2-sensitive
nanoparticles,
which
enable
measurements
oxygenation
on
conventional
fluorescence
microscope.
Nanosensor
probes,
termed
"MMIR"
(multimodal
infrared),
incorporate
an
NIR
metalloporphyrin
(PtTPTBPF)
deep
red
aza-BODIPY
reference
dyes
biocompatible
polymer
shell,
allowing
for
oxygen
gradient
quantification
via
ratio
phosphorescence
lifetime
readouts.
We
optimized
techniques
evaluated
nanosensor
probe
characteristics
cytotoxicity.
Subsequently,
applied
nanosensors
to
live
based
HCT116,
DPSCs,
SKOV3
cells,
at
rest,
treated
drugs
affecting
respiration.
found
that
growth
medium
viscosity,
size,
formation
method
influenced
oxygenation.
Some
spheroids
produced
from
HCT116
dental
pulp
stem
cells
exhibited
"inverted"
higher
core
levels
than
periphery.
This
contrasted
frequently
encountered
"normal"
hypoxia
toward
caused
by
diffusion.
Further
microscopy
analysis
demonstrated
metabolic
stratification
spheroids:
thus,
autofluorescence
FLIM
NAD(P)H
indicated
glycolytic
localization
OxPhos-active
Collectively,
demonstrate
strong
potential
NIR-emitting
ratiometric
advanced
studies
targeting
quantitative
real-time
monitoring
metabolism
in
complex
tissue
models.
ACS Sensors,
Journal Year:
2024,
Volume and Issue:
9(7), P. 3466 - 3488
Published: July 11, 2024
Organ-on-a-Chip
(OOC)
technology,
which
emulates
the
physiological
environment
and
functionality
of
human
organs
on
a
microfluidic
chip,
is
undergoing
significant
technological
advancements.
Despite
its
rapid
evolution,
this
technology
also
facing
notable
challenges,
such
as
lack
vascularization,
development
multiorgan-on-a-chip
systems,
replication
body
single
chip.
The
progress
has
played
crucial
role
in
steering
OOC
toward
mimicking
microenvironment,
including
microenvironment
replication,
multiorgan
microphysiological
systems.
Additionally,
advancements
detection,
analysis,
organoid
imaging
technologies
have
enhanced
efficiency
Organs-on-Chips
(OOCs).
In
particular,
integration
artificial
intelligence
revolutionized
imaging,
significantly
enhancing
high-throughput
drug
screening.
Consequently,
review
covers
research
Human-on-a-chip,
sensors
OOCs,
latest
applications
biomedical
field.
Scientific Reports,
Journal Year:
2025,
Volume and Issue:
15(1)
Published: Jan. 15, 2025
Abstract
Soil-transmitted
helminths
(STH)
are
widespread,
with
Ascaris
lumbricoides
infecting
millions
globally.
Malaria
and
STH
co-infections
common
in
co-endemic
regions.
Artemisinin
derivatives
(ARTs)—artesunate,
artemether,
dihydroartemisinin—are
standard
malaria
treatments
also
known
to
influence
the
energy
metabolism
of
parasites,
tumors,
immune
cells.
Herein,
we
explore
potential
ARTs
ascariasis
either
by
directly
targeting
larvae
or
indirectly
modifying
macrophage
responses.
suum
third-stage
porcine
IL-4
polarized
(M2-like)
macrophages
were
exposed
vitro,
their
was
evaluated
using
two-photon
NAD(P)H-FLIM.
Both
M2-like
exhibited
a
steady-state
bioenergetic
profile
high
oxidative
phosphorylation
low
anaerobic
glycolysis.
In
A.
larvae,
two
metabolically
distinct
regions
identified,
particularly
DUOX
activity
pharynx
compared
midgut;
however,
did
not
alter
these
profiles.
contrast,
exposure
induced
metabolic
shift
towards
glycolysis
reduced
activity,
suggesting
possible
indirect
effect
on
helminth
infection.
Overall,
NAD(P)H-FLIM
proved
be
powerful
tool
for
studying
specific
pathways
host
macrophages,
offering
valuable
insights
into
mechanisms
drug
action
both
parasite
host.
Methods in microscopy,
Journal Year:
2025,
Volume and Issue:
unknown
Published: March 19, 2025
Abstract
We
present
a
protocol
for
micro-endoscopic
fluorescence
lifetime
imaging
in
the
femoral
marrow
of
mice
allowing
analysis
NAD(P)H-dependent
metabolism
at
single
cell
level,
vivo
.
Therefore,
we
employed
gradient
refractive
index
(GRIN)
lens
system
fixed
to
mouse
femur
by
specialized
implant.
provide
step-by-step
instructions
practical
usage
microendoscopic
implant
and
discuss
experimental
parameters
required
reliable
NAD(P)H
FLIM
analysis,
particularly
referring
photon
statistics
signal-to-noise
ratio.
Representative
results
indicate
metabolic
heterogeneity
both
tissue
environment
among
LysM
+
myeloid
cells
expect
here
presented
approach
enable
cellular
functions
dysfunctions,
beyond
metabolism,
providing
better
understanding
bone
biology,
health
disease.
Journal of Nanobiotechnology,
Journal Year:
2025,
Volume and Issue:
23(1)
Published: Jan. 16, 2025
Gynecologic
cancers
(GCs),
including
cervical
cancer
(CC),
ovarian
(OC),
endometrial
(EC),
as
well
vulvar
and
vaginal
cancers,
represent
major
health
threats
to
women,
with
increasing
incidence
rates
observed
globally.
Conventional
treatments,
such
surgery,
radiation
therapy,
chemotherapy,
are
often
hindered
by
challenges
drug
resistance
recurrence,
contributing
high
mortality
rates.
Organoid
technology
has
emerged
a
transformative
tool
in
research,
offering
vitro
models
that
closely
replicate
the
tumor
cell
architecture
heterogeneity
of
primary
cancers.
Tumor-derived
organoids
preserve
histological
molecular
characteristics
original
tumors,
making
them
invaluable
for
studying
biology,
pathways,
immune
microenvironment.
Furthermore,
play
crucial
role
biomarker
discovery,
screening,
development
personalized
therapeutic
strategies.
In
contrast
traditional
lines
patient-derived
xenograft
(PDX)
models,
gynecologic
accurately
mirror
genetic
mutations
specific
gene
expression
profiles
tumors.
This
review
provides
an
overview
recent
advancements
organoid
highlighting
their
contributions
understanding
disease
mechanisms,
facilitating
advancing
precision
medicine.
It
also
addresses
potential
technology,
focus
on
its
treatment
approaches
GCs.
