Recent advances and perspectives on the development of circular RNA cancer vaccines

npj Vaccines, Journal Year: 2025, Volume and Issue: 10(1)

Published: March 1, 2025

Engineered circular RNAs (circRNAs) are emerging as promising platforms for RNA-based vaccines in cancer treatment. We summarize the recent advances of design, synthesis, and delivery circRNA-based vaccines, highlight applications challenges circRNA therapy. Further enhancements required areas such antigen selection, targeted delivery, multidimensional crosstalks, clinical trial assessments to advance efficacy safety cancer.

Language: Английский

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Therapeutic Use of G4-Ligands in Cancer: State-of-the-Art and Future Perspectives

Pharmaceuticals, Journal Year: 2024, Volume and Issue: 17(6), P. 771 - 771

Published: June 13, 2024

G-quadruplexes (G4s) are guanine-rich non-canonical secondary structures of nucleic acids that were identified in vitro almost half a century ago. Starting from the early 1980s, these also observed eukaryotic cells, first at telomeric level and later regulatory regions cancer-related genes, RNAs within specific cell compartments such as lysosomes, mitochondria, ribosomes. Because involvement large number biological processes pathogenesis several diseases, including cancer, interest G4 targeting has exponentially increased last few years, great novel ligands have been developed. Notably, represent family heterogeneous molecules can exert their functions by recognizing, binding, stabilizing multiple ways. Regarding anti-cancer activity, efficacy was originally attributed to capability inhibit activity telomerase, an enzyme elongates telomeres promotes endless replication cancer cells. Thereafter, mechanisms through which antitumoral activities defined, induction DNA damage, control gene expression, regulation metabolic pathways, among others. Here, we provided perspective on structure function with particular emphasis potential role agents. In particular, critically examined problems associated clinical translation molecules, trying highlight main aspects should be taken into account during phases drug design development. Indeed, taking advantage successes failures, more recent technological progresses field, it would possible hypothesize development future valid option for those cancers still missing effective therapies.

Language: Английский

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Could Drug Delivery Systems Be Enhancers for Therapy against KRAS-driven Cancers?

Reviews and Advances in Chemistry, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 8, 2025

Language: Английский

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Capturing the Dynamic Integrity of Carbocyanine Fluorophores-based Lipid Nanoparticles by the FRET technique

Journal of Materials Chemistry B, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 1, 2025

Nanoparticles capable of dynamically reporting their structural integrity in real-time are a powerful tool to guide the design drug delivery technologies.

Language: Английский

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DELIVER: the core principles for the clinic translation of nanomedicines

Acta Pharmaceutica Sinica B, Journal Year: 2025, Volume and Issue: 15(2), P. 1196 - 1198

Published: Jan. 24, 2025

Language: Английский

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Current Status and Future Prospects of Lyotropic Liquid Crystals as a Nanocarrier Delivery System for the Treatment of Cancer

AAPS PharmSciTech, Journal Year: 2025, Volume and Issue: 26(2)

Published: Feb. 7, 2025

Language: Английский

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Nanotherapy of Glioblastoma—Where Hope Grows

International Journal of Molecular Sciences, Journal Year: 2025, Volume and Issue: 26(5), P. 1814 - 1814

Published: Feb. 20, 2025

Localization in the central nervous system, diffuse growth, presence of stem cells, and numerous resistance mechanisms, all make glioblastoma (GBM) an incurable tumor. The standard treatment GBM consisting surgery; radio- chemotherapy with temozolomide provides insufficient therapeutic benefit needs to be updated effective modern solutions. One most promising intensively explored approaches against is use nanotherapy. first, so far only, nanoparticle-based therapy approved for NanoThermTM. It based on iron oxide nanoparticles thermal ablation tumor a magnetic field. Numerous other types nanotherapies are being evaluated, including polymer lipid-based nanoformulations, nanodiscs, dendrimers, metallic, silica, or bioderived nanoparticles, among others. advantages these nanoscale drug carriers include improved penetration across blood-brain barrier, targeted delivery, biocompatibility, lower systemic toxicity, while major problems their implementation involve scaling up production high costs. Nevertheless, taking impressive benefits into consideration, it seems obvious that combined effort scientific world will need taken tackle challenges implement novel therapies clinics, giving hope battle can finally won.

Language: Английский

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A carrier-free long-acting ropivacaine formulation using methylprednisolone sodium succinate as a dual-functional adjuvant

Theranostics, Journal Year: 2025, Volume and Issue: 15(9), P. 3862 - 3876

Published: March 3, 2025

Rationale: Slow-releasing formulation of local anesthetics (LAs) has been a promising strategy for pursuing long-acting opioid-free analgesia. Although many formulations based on carrier materials have successfully developed, challenges still remain in addressing not only the biocompatibility and biodegradability issues materials, but also potential inflammation caused by LAs. Methods: In this study, we developed slow-releasing ropivacaine methylprednisolone sodium succinate (MP), small-molecule drug used clinic. Firstly, studied self-assembling behavior MP its interaction with hydrochloride (RH). Then how could manipulate crystallization RH release profile obtained crystals be controlled. Lastly, investigated analgesic effect safety different animal models. Meanwhile monitored anti-inflammatory cell levels. Results: self-assemble into nanoparticles, which adsorb induce formation homogeneous microcrystals. Higher ratio system led to smaller microcrystals moderate rate, generated much longer reliable models considerable safety. On other hand, showed substantial effect, was helpful further relieve pain alleviate toxicity. Conclusion: Using as dual-functional adjuvant, LA prepared, providing facile solution long-term management

Language: Английский

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Direct structural investigation of pH responsiveness in mRNA lipid nanoparticles: Refining paradigms

Journal of Controlled Release, Journal Year: 2025, Volume and Issue: 384, P. 113848 - 113848

Published: May 20, 2025

Language: Английский

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Development of an Electrochemical Biosensor to Detect miRNA Encapsulated in Lipid Nanoparticles

Analytical Chemistry, Journal Year: 2025, Volume and Issue: unknown

Published: May 24, 2025

Lipid nanoparticles (LNPs) represent a versatile delivery platform proposed for wide range of therapies based on nucleic acids, including microRNA (miRNAs). The ability LNPs to encapsulate and protect RNA from degradation, as well their promote cellular uptake, has led clinical use with the approval RNA-based medicinal products, i.e., COVID vaccines. In this context, growing number LNP formulations improved transfection biocompatibility are under development, requiring rapid, sensitive, robust quality control tests, e.g., quantification encapsulated RNA. Nowadays, classical analytical approaches such fluorescence, ultraviolet-visible (UV-vis) spectrophotometry, chromatography mainly used drug. However, user-friendly cost-effective encapsulation efficacy within represents an important research focus, it would allow monitoring amount RNA, thus providing immediate control. work, we present adaptation electrochemical strip quantify miRNA, miR-218, whose antitumor effect been widely reported in literature LNPs. We provide rapid sensitive method assess concentrations miRNA actually encapsulated, obtaining satisfactory agreement compared traditional fluorimetric approach. Specifically, is commercial gold-screen-printed electrode modified DNA probe designed be fully complementary target miRNA-218. system was successfully combined 3D-printed chamber that allowed multiple electrodes simultaneously Triton X-100 surfactant disrupt release miRNA-218 achieving detection limit low 1 nM.

Language: Английский

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