An Immunomodulatory Hydrogel Featuring Antibacterial and Reactive Oxygen Species Scavenging Properties for Treating Periodontitis in Diabetes

Advanced Materials, Journal Year: 2024, Volume and Issue: unknown

Published: Nov. 28, 2024

Periodontal disease is a multifactorial, bacterially induced inflammatory disorder characterized by progressive destruction of periodontal tissues. Additionally, diabetes mellitus exacerbates periodontitis, resulting in expedited resorption bone. However, methods such as mechanical debridement, anti-inflammatory medications, and surgical approaches often fail to eradicate local infections inflammation, complicating the reconstruction tissue structures. Consequently, there an urgent need devise novel strategy for managing diabetic conditions. Here, multifunctional controlled-release drug delivery system (GOE1) developed encapsulating self-assembled nanoparticles (consisting chlorhexidine acetate epigallocatechin-3-gallate) into hydrogel matrix composed gelatin methacryloyl oxidized hyaluronic acid. In vitro experiments demonstrate that GOE1 possesses good antimicrobial, antioxidant properties, transgenic sequence genomics further illustrates IL-17-producing RAW 264.7 macrophages are critical mediating M1/M2 macrophage transition provide favorable immune microenvironment. addition, vivo reveal significantly ameliorates inflammation reduces loss alveolar bone reducing infiltration collagen destruction. Overall, offers promising therapeutic option periodontitis.

Language: Английский

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In situ forming gels as subcutaneous delivery systems of curcumin and piperine

Scientific Reports, Journal Year: 2025, Volume and Issue: 15(1)

Published: Jan. 24, 2025

In this study an in situ forming gel for curcumin and piperine delivery is investigated as a long-lasting strategy the local treatment of inflammatory degenerative joint disease, such osteoarthritis rheumatoid arthritis. Particularly glyceryl monooleate, association with phosphatidylcholine ethanol, were employed. Different ratios between excipients tested, aim to obtain liquid form suitable subcutaneous injection, gaining semisolid consistency contact biological fluids. A formulative was conducted assess composition impact on structural properties formulations, particularly focusing injectability phase transition. Curcumin loaded, singularly or jointly, selected gels. Structural characterization, performed by X-ray scattering, revealed disordered reverse micellar phases, undergoing transition hexagonal cubic Pn3m upon hydration. vitro dialysis release demonstrated sustained both drugs over 96 h, faster case jointly loaded drugs. Mechanistic analysis water uptake studies indicated drug governed diffusion swelling/erosion lipid supramolecular structure. Furthermore, ex vivo using human skin explants suggested formulation suitability indicating that presence formed allowed double respect simple gel.

Language: Английский

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Advanced Platelet-Rich Fibrin (A-PRF) as Antibiotics Delivery System: In-Vitro Proof-of-Concept Study

Materials, Journal Year: 2025, Volume and Issue: 18(3), P. 570 - 570

Published: Jan. 27, 2025

Autologous blood centrifugation produces various forms of platelet concentrates widely used in tissue regenerative therapies due to their high concentrations growth factors and abundance autologous cells. Advanced Platelet-Rich Fibrin (A-PRF), introduced as a low-speed product, contains an even higher concentration factors, greater number cells, looser fibrin clot structure compared previous Leukocyte (L-PRF). This study aims assess the potential A-PRF local delivery system for antibiotics. Different (0.5 mg/mL, 0.25 0.125 mg/mL) injectable amoxicillin (AMX) metronidazole (MTZ) were preliminarily tested impact on formation, with 0.5 mg/mL selected subsequent experiments. Blood samples from healthy volunteers supplemented antibiotics centrifuged form clots. Antibiotic-enriched clots immersed phosphate-buffered saline (1x PBS) analyzed at 24 h, 72 7 days, 14 days. AMX showed consistent release (mean: 19.9 ± 4.8 ng/mL h) over while MTZ demonstrated variability 12.8 4.5 h). remained constant 14-day period, no significant variations among patients. In contrast, displayed progressively lower time. Microbiological analysis revealed bacterial inhibition zones Fusobacterium nucleatum (AMX: 23 mm, MTZ: 28 mm) Prevotella intermedia 34 30 h. These findings suggest that can act effective antibiotic system, maintaining sustained antimicrobial activity potentially reducing need systemic

Language: Английский

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New Dawn in the Treatment of Rheumatoid Arthritis: Advanced Insight into Polymer Hydrogel Research

Gels, Journal Year: 2025, Volume and Issue: 11(2), P. 136 - 136

Published: Feb. 15, 2025

As a chronic systemic autoimmune disease, rheumatoid arthritis (RA) not only damages joints and other organs or systems throughout the body but also torments patients' physical mental health for long time, seriously affecting their quality of life. According to incomplete statistics at present, global prevalence RA is approximately 0.5-1%, number patients increasing year by year. Currently, drug therapies are usually adopted treatment RA, such as non-steroidal anti-inflammatory drugs (NSAIDs), disease-modifying antirheumatic (DMARDs), glucocorticoids/steroids, so on. However, traditional therapy has problems half-lives, cycles requiring frequent administration, lack specificity, possible adverse reactions (such gastrointestinal side effects, skin stratum corneum barrier damage, toxicity), which greatly restrict RA. In order improve limitations drug, physical, surgical treatments large related studies on have been carried out. Among them, hydrogels widely used in research due excellent biocompatibility, mechanical properties, general adaptability. For example, can be injected into synovial cavity fluid reduce wear between joints, lubricate avoid surface degradation. This article reviews applications under different functions situation carriers through delivery routes confirms outstanding potential great significance.

Language: Английский

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Design, fabrication and biocompatibility assessment of a carbon cloth-integrated MoS2-CuS-GO thermoresponsive hydrogel microsystem for photothermal-triggered benvitimod delivery

Nano Materials Science, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 1, 2025

Language: Английский

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Pure and Doped Brushite Cements Loaded with Piroxicam for Prolonged and Constant Drug Release

Materials, Journal Year: 2025, Volume and Issue: 18(5), P. 1065 - 1065

Published: Feb. 27, 2025

The increase in life expectancy has led to a rise of musculoskeletal disorders. Calcium phosphate cements (CPCs), thanks some amazing features such as the ability harden vivo, bioactivity, and resorbability, are promising candidates treat these diseases, notwithstanding their poor mechanical properties. We aimed synthesise pure barium- or silicon-doped brushite-based CPCs loaded with piroxicam study effects substitution on physical-chemical pharmaceutical properties before after cement immersion buffer for different time periods. Our results demonstrated that became amorphous hardened cements. dopants did not change brushite structure its lamellar morphology, while both Ba Si additions improved initial Young's modulus compared cement, opposite trend was observed compressive strength. Both strength elastic decreased samples immersed solution non-immersed samples, stabilisation number days increased. After 7 days, whole drug amount released, slower constant kinetic Ba-doped Si-doped ones.

Language: Английский

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Tumor microenvironment-activated and near-infrared light-driven free radicals amplifier for tetra-modal cancer imaging and synergistic treatment

Journal of Colloid and Interface Science, Journal Year: 2025, Volume and Issue: 689, P. 137208 - 137208

Published: March 1, 2025

Language: Английский

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Dual-responsive synthetic gene circuit for dynamic biologic drug delivery via inflammatory and circadian signaling pathways

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2025, Volume and Issue: unknown

Published: March 20, 2025

Engineered cells provide versatile tools for precise, tunable drug delivery, especially when synthetic stimulus-responsive gene circuits are incorporated. In many complex disease conditions, endogenous pathologic signals such as inflammation can vary dynamically over different time scales. For example, in autoimmune conditions rheumatoid arthritis or juvenile idiopathic arthritis, local (joint) and systemic inflammatory fluctuate daily, peaking the early morning, but also persist long periods of time, triggering flare-ups that last weeks to months. However, treatment with disease-modifying anti-rheumatic drugs is typically provided at continuous high doses, regardless activity without consideration levels signals. previous studies, we have developed cell-based delivery systems automatically address scales flares using either chronogenetic (i.e., clock gene-responsive elements) be tuned optimal dampen circadian variations inflammation-responsive NF-κB-sensitive respond sustained on demand proportional synthesis drug. The goal this study was develop a novel dual-responsive circuit responds both inputs OR-gate logic daily timed therapeutic output enhanced during chronic conditions. We driven by tandem NF-κB E'-box response elements. When engineered into induced pluripotent stem were chondrogenically differentiated, demonstrated basal-level an challenge shown bioluminescence monitoring. Similarly, system exhibited biologic interleukin-1 receptor antagonist (IL-1Ra) differentiated cartilage pellets. This mitigated measured reporter tissue-level degradation mimicking arthritic flare. herein input cues from two key homeostatic transcriptional networks, enabling dynamic output. proof-of-concept approach has potential match outcomes addresses environment arthritis.

Language: Английский

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Advancing Eco-friendly Biomedicine: Sustainable Nanocomposites as Platforms for Genetic Vaccines

Published: Jan. 1, 2025

Language: Английский

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Nanotechnology-Driven Drug Delivery in Rheumatoid Arthritis: Current Trends, Key Challenges, and Future Perspectives

Biosciences Biotechnology Research Asia, Journal Year: 2025, Volume and Issue: 22(1), P. 1 - 21

Published: March 25, 2025

ABSTRACT: Rheumatoid arthritis is a chronic autoimmune disorder that causes progressive joint damage and disability, significantly impacting patients' quality of life. In this Traditional treatment, including disease-modifying ant rheumatic drugs biologics often fall short in targeting the disease with precision, leading to side effects limited efficacy. We selected Nanotechnology which offers promising solution for improving drug delivery enhancing therapeutic outcomes RA management. This review explores current trends future prospects nanotechnology-based systems treatment. discuss various types nanocarriers, such as nanoparticles, liposomes, micelles, their ability enhance targeted biologic agents small molecules. Additionally, we examine potential "smart" nanomaterials respond disease-specific stimuli controlled release, thereby reducing systemic toxicity. The integration nanotechnology biologics, gene therapies, combination treatments highlighted strategy efficacy minimizing adverse effects. Finally, addresses ongoing challenges directions, need more extensive clinical trials ensure safety effectiveness these innovative therapies patients.

Language: Английский

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