Mitochondrial GPX4 acetylation is involved in cadmium-induced renal cell ferroptosis

Redox Biology, Journal Year: 2024, Volume and Issue: 73, P. 103179 - 103179

Published: May 8, 2024

Increasing evidences demonstrate that environmental stressors are important inducers of acute kidney injury (AKI). This study aimed to investigate the impact exposure Cd, an stressor, on renal cell ferroptosis. Transcriptomics analyses showed arachidonic acid (ARA) metabolic pathway was disrupted in Cd-exposed mouse kidneys. Targeted metabolomics oxidized ARA metabolites were increased mice. Renal 4-HNE, MDA, and ACSL4, upregulated Consistent with animal experiments, vitro experiments mitochondrial lipids elevated HK-2 cells. Ultrastructure membrane rupture Mitochondrial cristae accordingly reduced SIRT3, NAD

Language: Английский

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Absolute quantification of nicotinamide mononucleotide in biological samples by double isotope-mediated liquid chromatography-tandem mass spectrometry (dimeLC-MS/MS)

npj Aging, Journal Year: 2024, Volume and Issue: 10(1)

Published: Jan. 2, 2024

Abstract Nicotinamide adenine dinucleotide (NAD + ) is an essential metabolite for fundamental biological phenomena, including aging. mononucleotide (NMN) a key NAD intermediate that has been extensively tested as effective -boosting compound in mice and humans. However, the accurate measurement of NMN samples long challenge field. Here, we have established accurate, quantitative methodology measuring by using liquid chromatography-triple quadrupole mass spectrometry (LC-MS/MS) with double isotopic standards. In this new methodology, matrix effects were properly adjusted, fate could be traced during sample processing. We demonstrated can accurately quantitate levels mouse plasma confirmed quick, direct uptake into blood circulation cells. This d ouble i sotope- me diated LC-MS/MS (dimeLC-MS/MS) easily expanded to other -related metabolites reliable standard biology.

Language: Английский

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Supplementing Boar Diet with Nicotinamide Mononucleotide Improves Sperm Quality Probably through the Activation of the SIRT3 Signaling Pathway

Antioxidants, Journal Year: 2024, Volume and Issue: 13(5), P. 507 - 507

Published: April 24, 2024

Sperm quality is an important indicator to evaluate the reproduction ability of animals. Nicotinamide mononucleotide (NMN) participates in cell energy metabolism and reduces oxidative stress. However, effect regulatory mechanism NMN on porcine sperm are still unknown. Here, 32 Landrace boars were randomly assigned four groups (n = 8) fed with different levels (0, 8, 16 or mg/kg/d) for 9 weeks, then serum semen samples collected investigate function molecular quality. The results showed that dietary supplementation significantly increased volume, density motility (p < 0.05). Interestingly, apparently improved antioxidative indexes testosterone 0.05) serum. Furthermore, upregulated protein sirtuin 3 (SIRT3), antioxidation phosphorylation (OXPHOS), but downregulated apoptosis semen. Mechanically, protected from H2O2-induced stress through SIRT3 deacetylation. Importantly, SIRT3-specific inhibitor 3-TYP attenuated antiapoptosis sperm. Therefore, exerts improve boar via signaling pathway. Our findings suggest a novel potential feed additive produce high-quality

Language: Английский

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Nicotinamide Mononucleotide and Nicotinamide Riboside Reverse Ovarian Aging in Rats Via Rebalancing Mitochondrial Fission and Fusion Mechanisms

Pharmaceutical Research, Journal Year: 2024, Volume and Issue: 41(5), P. 921 - 935

Published: April 29, 2024

Abstract Purpose This study examined the effects of nicotinamide mononucleotide (NMN) and riboside (NR) on folliculogenesis mitochondrial dynamics (fission fusion mechanisms) in ovaries middle-aged female rats. Methods Experimental groups were young, (control), + NMN NR. was administered at a concentration 500 mg/kg intraperitoneally but NR 200 by gavage. Follicle stimulating hormone (FSH) luteinizing (LH) levels analyzed ELISA. Hematoxylin-eosin staining sections used for histopathological examination follicles-counting. Expression fission (Drp1, Mff Fis1) (Mfn1, Mfn2, Opa1, Fam73a Fam73b) genes as well Sirt1 gene RT-PCR. fission-related proteins (DRP1, MFF, FIS1 SIRT1) Western Blot. Results Higher ovarian index, more corpus luteum antral follicles detected compared to control. or could rebalance LH/FSH ratio. The control group determined possess higher expression lower when young group. In comparison with group, both found exhibit less fussion. protein measured Conclusion reveals that alone can decreasing excessive rat ovaries, thus alleviating stress correcting aging-induced abnormalities.

Language: Английский

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Mitochondrial dysfunction-associated alveolar epithelial senescence is involved in CdCl2-induced COPD-like lung injury

Journal of Hazardous Materials, Journal Year: 2024, Volume and Issue: 476, P. 135103 - 135103

Published: July 4, 2024

Language: Английский

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Mitochondrial Dysfunction‐Evoked DHODH Acetylation is Involved in Renal Cell Ferroptosis during Cisplatin‐Induced Acute Kidney Injury

Advanced Science, Journal Year: 2024, Volume and Issue: 11(43)

Published: Sept. 20, 2024

Several studies have observed renal cell ferroptosis during cisplatin-induced acute kidney injury (AKI). However, the mechanism is not completely clear. In this study, oxidized arachidonic acid (AA) metabolites are increased in cisplatin-treated HK-2 cells. Targeted metabolomics showed that end product of pyrimidine biosynthesis decreased and initiating substrate mouse kidneys. Mitochondrial DHODH, a key enzyme for synthesis, its downstream CoQH2, downregulated. DHODH overexpression attenuated but silence exacerbated CoQH2 depletion lipid peroxidation. Mechanistically, acetylation elevated cisplatin-exposed mice. SIRT3 reduced kidneys Both vitro vivo NMN supplementation mitochondrial ferroptosis. By contrast, Sirt3 knockout aggravated ferroptosis, which can be by NMN. Additional experiments cisplatin caused dysfunction SUMOylation. Pretreatment with mitochondria-target antioxidant MitoQ alleviated cisplatin-caused dysfunction, SUMOylation, acetylation. pretreatment protected against AKI Taken together, these results suggest dysfunction-evoked partially contributes to AKI.

Language: Английский

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Use of Nicotinamide Mononucleotide as Non-Natural Cofactor

Catalysts, Journal Year: 2025, Volume and Issue: 15(1), P. 37 - 37

Published: Jan. 3, 2025

Nicotinamide mononucleotide (NMN) has emerged as a promising non-natural cofactor with significant potential to transform biocatalysis, synthetic biology, and therapeutic applications. By modulating NAD⁺ metabolism, NMN offers unique advantages in enzymatic reactions, metabolic engineering, regenerative medicine. This review provides comprehensive analysis of NMN’s biochemical properties, mechanisms action, diverse Emphasis is placed on its role addressing challenges multi-enzyme cascades, biofuel production, the synthesis high-value chemicals. The paper also highlights critical research gaps, including need for scalable methods, improved integration into systems, toxicity studies use. Emerging technologies such AI-driven enzyme design CRISPR-based genome engineering are discussed transformative tools optimizing NMN-dependent pathways. Furthermore, synergistic biology innovations, cell-free systems dynamic regulatory networks, explored, paving way precise modular biotechnological solutions. Looking forward, versatility positions it pivotal tool advancing sustainable bioprocessing precision Addressing current limitations through interdisciplinary approaches will enable redefine boundaries innovation. serves roadmap leveraging across scientific industrial domains.

Language: Английский

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Nicotinamide: A Multifaceted Molecule in Skin Health and Beyond

Medicina, Journal Year: 2025, Volume and Issue: 61(2), P. 254 - 254

Published: Feb. 1, 2025

Nicotinamide (NAM), the amide form of vitamin B3, is a precursor to essential cofactors nicotinamide adenine dinucleotide (NAD⁺) and NADPH. NAD⁺ integral numerous cellular processes, including metabolism regulation, ATP production, mitochondrial respiration, reactive oxygen species (ROS) management, DNA repair, senescence, aging. NAM supplementation has demonstrated efficacy in restoring energy, repairing damage, inhibiting inflammation by suppressing pro-inflammatory cytokines release. Due its natural presence variety foods excellent safety profile—even at high doses up 3 g/day—NAM extensively used chemoprevention non-melanoma skin cancers treatment dermatological conditions such as blistering diseases, atopic dermatitis, rosacea, acne vulgaris. Recently, anti-aging properties have elevated NAM’s prominence skincare formulations. Beyond repair energy replenishment, significantly impacts oxidative stress reduction, cell cycle apoptosis modulation. Despite these multifaceted benefits, comprehensive molecular mechanisms underlying actions remain not fully elucidated. This review consolidates recent research shed light on mechanisms, emphasizing critical role health therapeutic potential. By enhancing our understanding, this work underscores importance continued exploration into applications, aiming inform future clinical practices innovations.

Language: Английский

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Fucoidan as an Encapsulant material: Applications and advantages in active agents and probiotic encapsulations

Food Chemistry, Journal Year: 2025, Volume and Issue: 477, P. 143570 - 143570

Published: Feb. 23, 2025

Language: Английский

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β-Nicotinamide mononucleotide blocks UVB-induced collagen reduction via regulation of ROS/MAPK/AP-1 and stimulation of mitochondrial proline biosynthesis

Photochemical & Photobiological Sciences, Journal Year: 2025, Volume and Issue: unknown

Published: March 1, 2025

Language: Английский

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