ACS Applied Materials & Interfaces,
Journal Year:
2024,
Volume and Issue:
16(29), P. 37418 - 37434
Published: July 9, 2024
The
re-epithelialization
process
gets
severely
dysregulated
in
chronic
nonhealing
diabetic
foot
ulcers/wounds.
Keratinocyte
growth
factor
(KGF
or
FGF-7)
is
the
major
modulator
of
process,
which
regulates
physiological
phenotypes
cutaneous
keratinocytes.
existing
therapeutic
strategies
administration
have
several
limitations.
To
overcome
these,
we
designed
a
KGF-mimetic
peptide
(KGFp,
13mer)
based
on
receptor
interaction
sites
murine
KGF.
KGFp
enhanced
migration
and
transdifferentiation
mouse
bone
marrow-derived
MSCs
toward
keratinocyte-like
cells
(KLCs).
A
significant
increase
expression
skin-specific
markers
Bnc1
(28.5-fold),
Ck5
(14.6-fold),
Ck14
(26.1-fold),
Ck10
(187.7-fold),
epithelial
EpCam
(23.3-fold)
Cdh1
(64.2-fold)
was
associated
with
activation
ERK1/2
STAT3
molecular
signaling
KLCs.
Further,
to
enhance
stability
wound
microenvironment,
it
conjugated
biocompatible
3D
porous
polymer
scaffolds
without
compromising
its
active
binding
followed
by
chemical
characterization
using
Fourier
transform
infrared
spectroscopy,
field-emission
scanning
electron
microscopy,
dynamic
mechanical
analysis,
thermogravimetry.
In
vitro
evaluation
KGFp-conjugated
revealed
potential
for
into
Transplantation
allogeneic
MSCGFP
type
2
wounds
(db/db
transgenic,
50-52
weeks
old
male
mice)
significantly
re-epithelialization-mediated
closure
rate
(79.3%)
as
compared
control
groups
(Untransplanted
-22.4%,
MSCGFP-3D
scaffold
-38.5%).
Thus,
drive
fate
keratinocytes
that
may
serve
stem
cell
delivery
platform
technology
tissue
engineering
transplantation.
ACS Applied Bio Materials,
Journal Year:
2024,
Volume and Issue:
7(8), P. 5082 - 5106
Published: July 15, 2024
In
view
of
their
exceptional
approach,
excellent
inherent
biocompatibility
and
biodegradability
properties,
interaction
with
the
local
extracellular
matrix,
protein-based
polymers
have
received
attention
in
bone
tissue
engineering,
which
is
a
multidisciplinary
field
that
repairs
regenerates
fractured
bones.
Bone
multihierarchical
complex
structure,
it
performs
several
essential
biofunctions,
including
maintaining
mineral
balance
structural
support
protecting
soft
organs.
Protein-based
gained
interest
developing
ideal
scaffolds
as
emerging
biomaterials
for
healing
regeneration,
challenging
to
design
substitutes
perfect
biomaterials.
Several
polymers,
collagen,
keratin,
gelatin,
serum
albumin,
etc.,
are
potential
materials
due
cytocompatibility,
controlled
biodegradability,
high
biofunctionalization,
tunable
mechanical
characteristics.
While
numerous
studies
indicated
encouraging
possibilities
proteins
BTE,
there
still
major
challenges
concerning
stability
physiological
conditions,
continuous
release
growth
factors
bioactive
molecules.
Robust
derived
from
can
be
used
replace
broken
or
diseased
biocompatible
substitute;
proteins,
being
biopolymers,
provide
engineering.
Herein,
recent
developments
protein
cutting-edge
engineering
addressed
this
review
within
3–5
years,
focus
on
significant
future
perspectives.
The
first
section
discusses
fundamentals
anatomy
scaffolds,
second
describes
fabrication
techniques
scaffolds.
third
highlights
importance
applications
BTE.
Hence,
development
state-of-the-art
has
been
discussed,
highlighting
Polymers,
Journal Year:
2024,
Volume and Issue:
16(4), P. 531 - 531
Published: Feb. 16, 2024
The
study
explores
the
in
vitro
biocompatibility
and
osteoconductivity
of
poly(methyl
methacrylate)/nano-hydroxyapatite
(PMMA/nHA)
composite
nanofibrous
scaffolds
for
bone
tissue
engineering
(BTE).
Electrospun
scaffolds,
exhibiting
both
low
high
fiber
orientation,
were
investigated.
inclusion
hydroxyapatite
nanoparticles
enhances
while
maintaining
ease
fabrication
through
electrospinning.
SEM
analysis
confirms
high-quality
morphology
with
successful
incorporation
nHA
evidenced
by
SEM-EDS
FTIR
methods.
DSC
indicates
that
addition
increases
PMMA
glass
transition
temperature
(Tg)
reduces
stress
relaxation
during
Furthermore,
higher
orientation
affects
Tg
differently.
Biological
studies
demonstrate
material’s
non-toxicity,
excellent
osteoblast
viability,
attachment,
spreading,
proliferation.
Overall,
PMMA/nHA
show
promise
BTE
applications.
Scientific Reports,
Journal Year:
2024,
Volume and Issue:
14(1)
Published: Nov. 2, 2024
Abstract
Severe
cutaneous
injuries
may
not
heal
spontaneously
and
necessitate
the
use
of
supplementary
therapeutic
methods.
Electrospun
nanofibers
possess
high
porosity
specific
surface
area,
which
provide
necessary
microenvironment
for
wound
healing.
Here
in,
Soluplus-soy
protein
isolate
(Sol-SPI)
containing
mupirocin
(Mp)
were
fabricated
via
electrospinning
treatment.
The
exhibited
water
absorption
capacities
about
300.83
±
29.72%
vapor
permeability
values
821.8
49.12
g/m
2
day.
Sol/SPI/Mp
showed
an
in
vitro
degradability
33.73
3.55%
after
5
days.
ultimate
tensile
strength,
elastic
modulus,
elongation
measured
as
3.61
0.29
MPa,
39.15
5.08
59.11
1.94%,
respectively.
Additionally,
85.90
6.02%
Mp
loaded
was
released
days
vitro,
by
applying
Mp-loaded
nanofibers,
93.06
5.40%
90.40
5.66%
S.
aureus
E.
coli
bacteria
killed,
Human
keratinocyte
cells
(HaCat)
demonstrated
notable
biocompatibility
with
prepared
nanofibers.
Furthermore,
compare
to
other
groups,
Sol-SPI-Mp
caused
fastest
re-epithelialization
healing
a
rat
model.
findings
this
study
present
novel
nanofiber-based
dressing
that
accelerates
severe
skin
wounds
risk
infection.
Journal of Nanobiotechnology,
Journal Year:
2024,
Volume and Issue:
22(1)
Published: Sept. 2, 2024
Improper
management
of
diabetic
wound
effusion
and
disruption
the
endogenous
electric
field
can
lead
to
passive
healing
damaged
tissue,
affecting
process
tissue
cascade
repair.
This
study
developed
an
extracellular
matrix
sponge
scaffold
(K1P6@Mxene)
by
incorporating
Mxene
into
acellular
dermal
stroma-hydroxypropyl
chitosan
interpenetrating
network
structure.
is
designed
couple
with
promote
precise
remodelling
in
wounds.
The
fibrous
structure
closely
resembles
that
a
natural
matrix,
providing
conducive
microenvironment
for
cells
adhere
grow,
exchange
oxygen.
Additionally,
inclusion
enhances
antibacterial
activity(98.89%)
electrical
conductivity
within
scaffold.
Simultaneously,
K1P6@Mxene
exhibits
excellent
water
absorption
(39
times)
porosity
(91%).
It
actively
interacts
guide
cell
migration
growth
on
surface
upon
absorbing
exudate.
In
vivo
experiments,
reduced
inflammatory
response
wounds,
increased
collagen
deposition
arrangement,
promoted
microvascular
regeneration,
Facilitate
expedited
re-epithelialization
minimize
scar
formation,
accelerate
wounds
7
days.
Therefore,
this
scaffold,
combined
field,
presents
appealing
approach
comprehensive
repair
Journal of Biomedical Materials Research Part A,
Journal Year:
2024,
Volume and Issue:
112(10), P. 1675 - 1687
Published: April 10, 2024
The
physiological
mechanism
of
bone
tissue
regeneration
is
intricately
organized
and
involves
several
cell
types,
intracellular,
extracellular
molecular
signaling
networks.
To
overcome
the
drawbacks
autografts
allografts,
a
number
synthetically
produced
scaffolds
have
been
manufactured
by
integrating
ceramics,
polymers,
their
hybrid-composites.
Considering
fact
that
natural
composed
primarily
collagen
hydroxyapatite,
ceramic-polymer
composite
materials
seem
to
be
most
viable
alternative
implants.
Here,
in
this
experimental
study,
copolymer
PVDF-TrFE
has
amalgamated
with
HA
ceramics
produce
as
In
order
fabricate
PVDF-TrFE-HA
(polyvinylidene
fluoride-trifluoroethylene-hydroxyapatite)
scaffolds,
solvent
casting-particulate
leaching
technique
was
devised.
Two
scaffold
specimens
were
produced,
different
molar
ratios
(70:30
50:50),
then
electrically
polarized
observe
subsequent
polarization
impact
on
growth
suppression
bacterial
proliferation.
Both
underwent
characterization
analyze
biocompatibility
bactericidal
activities.
culture
Pseudomonas
aeruginosa
(P.
aeruginosa)
Staphylococcus
aureus
(S.
aureus)
bacteria
composites
studied
understand
antibacterial
characteristics.
Moreover,
MG63
cells
cultured
these
as-formed
provided
information
about
osteogenesis.
Improved
osteogenesis
efficacy
observed
both
composites.
However,
70
wt%
30
showed
higher
effect
well
It
found
PVDF-TrFE-HA-based
biomaterials
potential
for
engineering
applications.
