3D Porous Polymer Scaffold-Conjugated KGF-Mimetic Peptide Promotes Functional Skin Regeneration in Chronic Diabetic Wounds DOI
Subholakshmi Choudhury,

Mangali Madhu Krishna,

Debanjan Sen

et al.

ACS Applied Materials & Interfaces, Journal Year: 2024, Volume and Issue: 16(29), P. 37418 - 37434

Published: July 9, 2024

The re-epithelialization process gets severely dysregulated in chronic nonhealing diabetic foot ulcers/wounds. Keratinocyte growth factor (KGF or FGF-7) is the major modulator of process, which regulates physiological phenotypes cutaneous keratinocytes. existing therapeutic strategies administration have several limitations. To overcome these, we designed a KGF-mimetic peptide (KGFp, 13mer) based on receptor interaction sites murine KGF. KGFp enhanced migration and transdifferentiation mouse bone marrow-derived MSCs toward keratinocyte-like cells (KLCs). A significant increase expression skin-specific markers Bnc1 (28.5-fold), Ck5 (14.6-fold), Ck14 (26.1-fold), Ck10 (187.7-fold), epithelial EpCam (23.3-fold) Cdh1 (64.2-fold) was associated with activation ERK1/2 STAT3 molecular signaling KLCs. Further, to enhance stability wound microenvironment, it conjugated biocompatible 3D porous polymer scaffolds without compromising its active binding followed by chemical characterization using Fourier transform infrared spectroscopy, field-emission scanning electron microscopy, dynamic mechanical analysis, thermogravimetry. In vitro evaluation KGFp-conjugated revealed potential for into Transplantation allogeneic MSCGFP type 2 wounds (db/db transgenic, 50-52 weeks old male mice) significantly re-epithelialization-mediated closure rate (79.3%) as compared control groups (Untransplanted -22.4%, MSCGFP-3D scaffold -38.5%). Thus, drive fate keratinocytes that may serve stem cell delivery platform technology tissue engineering transplantation.

Language: Английский

Incorporation of forsterite nanoparticles in a 3D printed polylactic acid/polyvinylpyrrolidone scaffold for bone tissue regeneration applications DOI

Nafise Kazemi,

S.A. Hassanzadeh-Tabrizi, Narjes Koupaei

et al.

International Journal of Biological Macromolecules, Journal Year: 2025, Volume and Issue: unknown, P. 141046 - 141046

Published: Feb. 1, 2025

Language: Английский

Citations

0
Extracellular vesicle-mediated gene therapy targets BRAFV600E-mutant colorectal cancer by inhibiting the MEK1/2-ERK1/2 pathway DOI Creative Commons
Di Wang, Liwei Wang, Wei Zhan

et al.

Journal of Nanobiotechnology, Journal Year: 2025, Volume and Issue: 23(1)

Published: Feb. 20, 2025

Patients with colorectal cancer (CRC) harboring BRAF mutation have a poor prognosis. The median survival time for patients advanced BRAFV600E-mutant CRC is only approximately one year. Owing to the insensitivity standard chemotherapy, there are still no effective and highly specific treatment strategies available in clinical practice mutation. Therefore, targeting BRAFV600E site, researching exploring novel targeted therapies essential improve rate of this subtype. This study aims develop precise therapeutic system CRC, based on carrier properties extracellular vesicles (EVs) gene therapy BRAFV600E. We first obtained engineered cells capable stably producing EVs loaded nucleic acid drugs (siBRAFV600E). Next, wild-type cell lines, as well corresponding subcutaneous metastasis models, were used evaluate efficacy EVs-siBRAFV600E explored mechanism. Notably, patient-derived xenograft (PDX) which share same molecular characteristics, pathological features, heterogeneity do, utilized further explore mechanisms. specifically inhibited but didn't affect vitro vivo. exerts its effect by regulating MEK1/2-ERK1/2 pathway, it has demonstrated excellent PDX models. we constructed CRC. provides strategy

Language: Английский

Citations

0
Advances in bioink-based 3D printed scaffolds: optimizing biocompatibility and mechanical properties for bone regeneration DOI
Pawan Kumar, Jitender Sharma, Ravinder Kumar

et al.

Biomaterials Science, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 1, 2025

The development of bioink-based 3D-printed scaffolds has revolutionized bone tissue engineering (BTE) by enabling patient-specific and biomimetic constructs for regeneration.

Language: Английский

Citations

0
Innovative Approaches in Bone Tissue Engineering: Strategies for Cancer Treatment and Recovery DOI Open Access

Samiksha Khobragade,

Manish Deshmukh,

Ujwal Vyas

et al.

International Journal of Molecular Sciences, Journal Year: 2025, Volume and Issue: 26(9), P. 3937 - 3937

Published: April 22, 2025

Cancer has rapidly emerged as a leading global cause of premature mortality, with significant economic implications projected to reach USD 25.2 trillion from 2020 2050. Among the various types cancer, primary bone cancers, though uncommon, are see nearly 4000 new cases diagnosed in United States 2024. The complexity treating cancer arises its rarity, diversity, and challenges associated surgical interventions, metastatic spread, post-operative complications. Advancements tissue engineering (BTE) have introduced innovative therapeutic approaches promote regeneration address tumor recurrence. This interdisciplinary field integrates biomaterials, scaffolds, gene therapy, utilizing technologies such 3D bioprinting create custom scaffolds that facilitate cellular activities essential for regeneration. Recent developments biodegradable, bioactive materials aim enhance biocompatibility effectiveness while nanotechnology presents promising avenues targeted drug delivery improved outcomes. review outlines current landscape BTE, highlighting scaffold fabrication techniques, advantages incorporating stem cell therapies, future directions, including integration artificial intelligence design personalized medicine orthopedic oncology. work underscores necessity ongoing research innovation, aiming improve strategies specifically designed unique posed by sarcomas cancers.

Language: Английский

Citations

0
Programmed wound healing in aged skin may be enhanced by mesenchymal cell loaded gene-activated scaffolds DOI Creative Commons
Priya Das, Martin Maresch,

Nigamananda Dey

et al.

APL Bioengineering, Journal Year: 2025, Volume and Issue: 9(2)

Published: April 25, 2025

Aging can prolong the wound healing and is associated with decline in stem cells, delays cellular migration, lower vascularization. Tissue engineering has largely evolved to incorporate advanced three-dimensional dressings, scaffolds, hydrogels that may be seeded mesenchymal stromal cells (MSCs) foster an environment conducive regeneration enhance process. The effectiveness of cell-seeded scaffolds improved by incorporating activating agents such as nucleic acids resulting gene-activated (GAS), thereby facilitating targeted aged patients. In this study, we assess vivo potential a promising MSC collagen scaffold, containing anti-fibrotic agent β-klotho pro-angiogenic derived factor (SDF-1α) male Sprague Dawley rats (20–24 month old). A cell loaded split skin model compared only clinical standard dressing +Jelonet, MSCs +gene-free +SDF-1α/β-klotho dual scaffold up 21 days. Our results showed all groups except +Jelonet which scab formation exudate. group healed primarily via fibrotic contraction. contrast, host tissue integration redistribution extracellular matrix proteins, less contraction, complete re-epithelized wounds at day 21. GAS displayed programmed greatest neo-vascularization CD31 expression. conclusion, effectively modulated when are on biocompatible particularly these factors. This approach enhances blood vessel while reducing fibrosis, suggesting for strategies chronic wounds.

Language: Английский

Citations

0
Additively manufactured functional polymers and nanocomposites for health care and biomedical applications DOI
Santosh Kumar,

Rakesh Kumar

Elsevier eBooks, Journal Year: 2025, Volume and Issue: unknown, P. 587 - 611

Published: Jan. 1, 2025

Language: Английский

Citations

0
Accelerated in-vivo infected dermal wound healing with antimicrobial Bio-nanocomposite hydrogel DOI Creative Commons
Preety Sharma, Saurav Sharma,

Maryam Zohour Soleimani

et al.

Alexandria Engineering Journal, Journal Year: 2024, Volume and Issue: 88, P. 230 - 244

Published: Jan. 18, 2024

Infected wounds pose significant challenges in clinical settings due to the risk of complications and delayed healing wounds. The most common wound dressings are cotton balls gauze, yet these traditional have inherent shortcomings including low antibacterial properties, less oxygen permeation, mechanical moisture content essential for infected Herein, we fabricate polyvinyl alcohol/chitosan/pectin (PVA/CH/PEC) hydrogel loaded with green-synthesized nanometals, synthetic antibiotics treatment enhanced synergistic higher moisture, permeation content. silver nanoparticles (AgNPs), synthesized via sea buckthorn leaf extract, ciprofloxacin were incorporated bio-nanocomposite hydrogels. particle size AgNPs was found be range 15 42 nm. hydrogels confirm its biocompatible nature because reduction percentage hemolysis from 1.7% 1.4% cell viability exceeding 87% PVA/CH/PEC poly vinyl alcohol/chitosan/pectin–Silver/ (PVA/CH/PEC-Ag/cipro) membranes, respectively. Microscopic observation PVA/CH/PEC/Ag-Ciprofloxacin demonstrated last stages regeneration skin reformation towards normal as compared scaffold without ciprofloxacin. promoted advanced reformation, signifying potential a promising dressing material applications.

Language: Английский

Citations

3
Mimic miRNA and Anti-miRNA Activated Scaffolds as a Therapeutic Strategy to Promote Bone, Cartilage, and Skin Regeneration DOI Creative Commons
Gabriella Guelfi, Camilla Capaccia, Polina Anipchenko

et al.

Macromol—A Journal of Macromolecular Research, Journal Year: 2024, Volume and Issue: 4(2), P. 165 - 189

Published: April 1, 2024

MiRNA-based therapies represent an innovative and promising strategy applicable to various medical fields, such as tissue regeneration the treatment of numerous diseases, including cancer, cardiovascular problems, viral infections. MiRNAs, a group small non-coding RNAs, play critical role in regulating gene expression at post-transcriptional level modulate several signaling pathways that maintain cellular homeostasis. The clinical trials discussed review herald new therapeutic era for miRNAs, particularly engineering, using synthetic exogenous mimic miRNAs antisense (anti-miRNAs) restore health. This provides overview miRNAs’ biogenesis, mechanism action, regulation, potential applications, followed by examination challenges associated with transport delivery miRNAs. possibility non-viral vectors protect against degradation ensure effective miRNA is highlighted, focusing on advantages emerging use 3D biomaterial scaffolds anti-miRNAs facilitate repair regeneration. Finally, assesses current landscape miRNA-activated scaffold preclinical studies bone, cartilage, skin tissues, emphasizing their emergence frontier personalized medicine.

Language: Английский

Citations

3
Finite element analysis and in vitro tests on endurance life and durability of composite bone substitutes DOI Creative Commons

Amir Abbas Seraji,

Reza Nahavandi,

Amir Kia

et al.

Frontiers in Bioengineering and Biotechnology, Journal Year: 2024, Volume and Issue: 12

Published: Sept. 5, 2024

Bone structures facilitate the regeneration and repair of bone tissue in regions where it has been damaged or destroyed, either temporarily permanently. Therefore, bone's fatigue strength durability are crucial to its efficacy longevity. Several variables, such as construct's material qualities, design, production procedure, loading unloading cycles, physiological conditions influence endurance life constructs. Metals, ceramics, polymers all routinely utilized create substitutes, each these materials unique features that might affect final product. The mechanical performance capacity promote may be affected by scaffold's porosity, pore size. Researchers employ testing under cyclic circumstances one example an experimental approach used assess construction endurance. These analyses can give us important information about stress-strain behavior, resistance multiple new structure. Predicting developed construct also possible with use simulations numerical analyses. Hence, order reliable efficient constructs for engineering, is understand their durability. purpose this study analyze effective parameters gather models evaluations assessments.

Language: Английский

Citations

3
AI-Based solutions for current challenges in regenerative medicine DOI

Pedram Asadi Sarabi,

Mahshid Shabanpouremam,

Amir Reza Eghtedari

et al.

European Journal of Pharmacology, Journal Year: 2024, Volume and Issue: unknown, P. 177067 - 177067

Published: Oct. 1, 2024

Language: Английский

Citations

3