Medicinal plant-derived mtDNA via nanovesicles induces the cGAS-STING pathway to remold tumor-associated macrophages for tumor regression

Journal of Nanobiotechnology, Journal Year: 2023, Volume and Issue: 21(1)

Published: March 6, 2023

Plant-derived nanovesicles (PDNVs) have been proposed as a major mechanism for the inter-kingdom interaction and communication, but effector components enclosed in vesicles mechanisms involved are largely unknown. The plant Artemisia annua is known an anti-malaria agent that also exhibits wide range of biological activities including immunoregulatory anti-tumor properties with to be further addressed. Here, we isolated purified exosome-like particles from A. annua, which were characterized by nano-scaled membrane-bound shape hence termed artemisia-derived (ADNVs). Remarkably, demonstrated inhibit tumor growth boost immunity mouse model lung cancer, primarily through remolding microenvironment reprogramming tumor-associated macrophages (TAMs). We identified plant-derived mitochondrial DNA (mtDNA), upon internalized into TAMs via vesicles, molecule induce cGAS-STING pathway driving shift pro-tumor phenotype. Furthermore, our data showed administration ADNVs greatly improved efficacy PD-L1 inhibitor, prototypic immune checkpoint tumor-bearing mice. Together, present study, first time, knowledge, unravels wherein medical mtDNA, nanovesicles, induces immunostimulatory signaling mammalian cells resetting promoting eradication.

Language: Английский

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A comprehensive review on recent advances in exosome isolation and characterization: Toward clinical applications

Translational Oncology, Journal Year: 2024, Volume and Issue: 50, P. 102121 - 102121

Published: Sept. 14, 2024

Language: Английский

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Plant-derived extracellular vesicles (PDEVs) in nanomedicine for human disease and therapeutic modalities

Journal of Nanobiotechnology, Journal Year: 2023, Volume and Issue: 21(1)

Published: March 29, 2023

Abstract Background The past few years have witnessed a significant increase in research related to plant-derived extracellular vesicles (PDEVs) biological and medical applications. Using biochemical technologies, multiple independent groups demonstrated the important roles of PDEVs as potential mediators involved cell-cell communication exchange bio-information between species. Recently, several contents been well identified PDEVs, including nucleic acids, proteins, lipids, other active substances. These cargoes carried by could be transferred into recipient cells remarkably influence their behaviors associated with human diseases, such cancers inflammatory diseases. Main body abstract This review summarizes latest updates regarding focuses on its role nanomedicine applications, drug delivery strategies develop diagnostic therapeutic agents for clinical management especially like cancers. Conclusion Considering unique advantages, high stability, intrinsic bioactivity easy absorption, further elaboration molecular mechanisms factors driving function will provide new horizons treatment disease.

Language: Английский

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Plant-Derived Exosome-like Nanoparticles for Biomedical Applications and Regenerative Therapy

Biomedicines, Journal Year: 2023, Volume and Issue: 11(4), P. 1053 - 1053

Published: March 29, 2023

Plant-derived exosome-like nanoparticles (PDENs) comprise various bioactive biomolecules. As an alternative cell-free therapeutic approach, they have the potential to deliver nano-bioactive compounds human body, and thus lead anti-inflammatory, antioxidant, anti-tumor benefits. Moreover, it is known that Indonesia one of herbal centers world, with abundance unexplored sources PDENs. This encouraged further research in biomedical science develop natural richness plants as a source for welfare. study aims verify PDENs purposes, especially regenerative therapy applications, by collecting analyzing data from latest relevant developments.

Language: Английский

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Potential of Plant Exosome Vesicles from Grapefruit (Citrus × paradisi) and Tomato (Solanum lycopersicum) Juices as Functional Ingredients and Targeted Drug Delivery Vehicles

Antioxidants, Journal Year: 2023, Volume and Issue: 12(4), P. 943 - 943

Published: April 17, 2023

Plant-derived extracellular vesicles (PEVs) have gained attention as promising bioactive nutraceutical molecules; their presence in common fruit juices has increased significance because human interaction is inevitable. The goal of this study was to evaluate the potential PEVs derived from grapefruit and tomato functional ingredients, antioxidant compounds, delivery vehicles. were isolated using differential ultracentrifugation found be similar size morphology mammalian exosomes. yield exosome-like (GEVs) higher than that (TEVs), despite latter having larger vesicle sizes. Furthermore, activity GEVs TEVs low comparison juice sources, indicating a limited contribution juice. showed efficiency being loaded with heat shock protein 70 (HSP70) TEVs, well TEV PEV-free HSP70 delivering glioma cells. Overall, our results revealed present ingredients they exert deliver molecules Although activity, role oxidative response cells should further addressed.

Language: Английский

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Plant exosomes fused with engineered mesenchymal stem cell‐derived nanovesicles for synergistic therapy of autoimmune skin disorders

Journal of Extracellular Vesicles, Journal Year: 2023, Volume and Issue: 12(10)

Published: Oct. 1, 2023

Existing therapeutics for autoimmune diseases remain problematic due to low efficacy, severe side effects, and difficulties reach target tissues. Herein, we design multifunctional fusion nanovesicles that can lesions the treatment of skin diseases. The grapefruit-derived exosome-like (GEVs) with anti-inflammatory antioxidant effects are first encapsulated CX5461, an immunosuppressant anti-proliferative properties form GEV@CX5461. In order enhance therapeutic efficiency safety, GEV@CX5461 then fused CCR6+ derived from membranes engineered gingiva-derived mesenchymal stem cells (GMSCs). resulting FV@CX5461 not only maintain bioactivity GEVs, GMSC but also home inflamed tissues rich in chemokine CCL20 through chemotaxis function CCR6 on FVs. Moreover, reduce secretion inflammatory factors, calm down Th17 cell activation, induce Treg infiltration. Finally, impressive both psoriasis atopic dermatitis disease models is demonstrated using reshape unbalanced immune microenvironment. A nanotherapeutic drug delivery strategy developed plant animal high clinical potential.

Language: Английский

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Plant-derived exosomes: a green approach for cancer drug delivery

Journal of Materials Chemistry B, Journal Year: 2024, Volume and Issue: 12(9), P. 2236 - 2252

Published: Jan. 1, 2024

Plant-derived exosomes (PDEs) show promising potential to be developed as a therapeutic agent against cancer, owing their multiple advantages such low toxicity, biocompatibility, availability, affordability, etc.

Language: Английский

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Research status and challenges of plant-derived exosome-like nanoparticles

Biomedicine & Pharmacotherapy, Journal Year: 2024, Volume and Issue: 174, P. 116543 - 116543

Published: April 11, 2024

In recent years, there has been an increasing number of related studies on exosomes. Most have focused exosomes derived from mammals, confirming the important role that play in cell communication. Plants, as a natural ingredient, plant-derived confirmed to similar structures and functions mammalian-derived Plant-derived exosome-like nanoparticles (PELNs) are lipid bilayer membrane nanovesicles containing bioactive constituents such miRNA, mRNA, protein, lipids obtained plant cells, can participate intercellular communication mediate transboundary communication, high bioavailability low immunogenicity, relatively safe, shown maintaining homeostasis preventing, treating variety diseases. this review, we describe biogenesis, isolation purification methods, structural composition, stability, safety, function PELNs challenges. The anti-inflammatory, antioxidant, antitumor drug delivery mainly described, status research exosome Chinese herbal medicines is outlined. Overall, summarized importance latest results field provided theoretical basis for future clinical application PELNs.

Language: Английский

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Isolation of plant-derived exosome-like nanoparticles (PDENs) from Solanum nigrum L. berries and Their Effect on interleukin-6 expression as a potential anti-inflammatory agent

PLoS ONE, Journal Year: 2024, Volume and Issue: 19(1), P. e0296259 - e0296259

Published: Jan. 4, 2024

Inflammation is a temporary response of the immune system that can be treated using common anti-inflammatory drugs. However, prolonged use these drugs increases risk adverse side effects. Accordingly, there an increasing need for alternative treatments inflammation with fewer Exosomes are extracellular vesicles secreted by most eukaryotic cells and have been studied as candidate cell-free therapy inflammatory diseases due to their immunomodulatory properties. In recent years, focus exosome research has shifted from animal cell-derived exosomes plant-derived exosome-like nanoparticles (PDENs). Plant-derived (PDENs) easier obtain, minimal safety concerns, produced in higher quantities lower cost than derived cells. this study, isolation analysis potential PDENs black nightshade berries ( Solanum nigrum L.) were carried out. The results characterization showed had spherical morphology, measuring around 107 nm zeta -0.6 mV, protein concentration 275.38 μg/mL. also shown internalized RAW264.7 macrophage cell line after 2 hours incubation no cytotoxicity effect up 2.5 Furthermore, exposure several doses LPS-stimulated significantly decreased expression pro-inflammatory cytokine gene IL-6, well IL-6 97,28%. GC-MS presence neral, monoterpene compound known properties, which may contribute activity isolated L. berries. Taken together, present study was first isolate characterize demonstrated PDEN suppressing production

Language: Английский

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Magnetic natural lipid nanoparticles for oral treatment of colorectal cancer through potentiated antitumor immunity and microbiota metabolite regulation

Biomaterials, Journal Year: 2024, Volume and Issue: 307, P. 122530 - 122530

Published: March 11, 2024

Language: Английский

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