A multi-strain human skin microbiome model provides a testbed for disease modeling DOI Creative Commons
Alan Maloney,

Timothy B. Crawford,

J. Benjamin Hurlbut

et al.

Frontiers in Microbiomes, Journal Year: 2025, Volume and Issue: 4

Published: Feb. 4, 2025

The skin microbiome plays a critical role at the interface between human epidermis and environment, providing colonization resistance against pathogenic strains, training host immunity, supporting epithelial turnover. Inversely, dysbiotic states are associated with disease, particularly inflammatory conditions such as atopic dermatitis psoriasis. Current evaluation of interactions relies on post hoc studies after disease onset. This limits ability to evaluate causal roles microbe during progression. One approach characterizing microbial biology in controlled reproducible context is derive vitro models sufficient complexity stability support perturbation response. tools for studying these processes focused testing antagonistic or synergistic relations two more strains short (hours days) culture durations, thereby precluding relevant chronic states. Here, we present an model comprising six strain consortium colonizing primary keratinocyte-derived tissue Air-Liquid Interface up 7 days. We evaluated readouts health including histology, gene expression, transepithelial electrical (TEER), well relative abundance characterize over time. Skin cells formed complex structure weeks maintained stable increasing TEER days co-culture consortium. Up five were viable surface day validated by custom qPCR assays, demonstrating robust testbed studies. A remarkable feature this persistence Cutibacterium acnes aerobic since C. growth typically demonstrated under anaerobic conditions, suggesting that conducive natural native strains. addition cytokines representative elicited marked decrease barrier compared healthy controls, irrespective presence. Furthermore, alteration was observed diseased tissues, capability study impact vice versa. envision system test bed influence commensals biology, external environment stability, diseases impacted dysbiosis.

Language: Английский

Gut microbiota in various childhood disorders: Implication and indications DOI Creative Commons
Nermin Kamal Saeed, Mohammed Al‐Beltagi, Adel Salah Bediwy

et al.

World Journal of Gastroenterology, Journal Year: 2022, Volume and Issue: 28(18), P. 1875 - 1901

Published: May 6, 2022

Gut microbiota has a significant role in gut development, maturation, and immune system differentiation. It exerts considerable effects on the child's physical mental development. The composition structure depend many host microbial factors. factors include age, genetic pool, general health, dietary factors, medication use, intestine's pH, peristalsis, transit time, mucus secretions, mucous immunoglobulin, tissue oxidation-reduction potentials. nutrient availability, bacterial cooperation or antagonism, adhesion. Each part of its due to specific characteristics. interacts with different body parts, affecting pathogenesis local systemic diseases. Dysbiosis is common finding childhood disorders such as autism, failure thrive, nutritional disorders, coeliac disease, Necrotizing Enterocolitis, helicobacter pylori infection, functional gastrointestinal childhood, inflammatory bowel diseases, other disorders. also observed allergic conditions like atopic dermatitis, rhinitis, asthma. can impact development progression cardiac including heart failure. Probiotic supplements could provide some help managing these However, we are still need more studies. In this narrative review, will shed light management

Language: Английский

Citations

77
Skin as the target for allergy prevention and treatment DOI Creative Commons
Andreina Marques‐Mejias, Irene Bartha, Christina E. Ciaccio

et al.

Annals of Allergy Asthma & Immunology, Journal Year: 2024, Volume and Issue: 133(2), P. 133 - 143

Published: Jan. 21, 2024

Language: Английский

Citations

19
Photo-responsive polydopamine nanoenzyme microneedles with oxidative stress regulation ability for atopic dermatitis treatment DOI
Yuanyuan Zhang, Xiao‐Xuan Zhang,

Xiangyi Wu

et al.

Nano Today, Journal Year: 2024, Volume and Issue: 56, P. 102241 - 102241

Published: March 28, 2024

Language: Английский

Citations

19
Scratching promotes allergic inflammation and host defense via neurogenic mast cell activation DOI
Andrew Liu,

Y. Zhang,

Chien-Sin Chen

et al.

Science, Journal Year: 2025, Volume and Issue: 387(6733)

Published: Jan. 30, 2025

Itch is a dominant symptom in dermatitis, and scratching promotes cutaneous inflammation, thereby worsening disease. However, the mechanisms through which exacerbates inflammation whether provides benefit to host are largely unknown. We found that was required for skin mouse models dependent on FcεRI-mediated mast cell activation. Scratching-induced pain-sensing nociceptors, neuropeptide substance P, receptor MrgprB2. Scratching also increased augmented defense superficial Staphylococcus aureus infection. Thus, activation of nociceptor-driven neuroinflammation, both exacerbated allergic disease provided protection from S. aureus, reconciling seemingly paradoxical role as pathological process evolutionary adaptation.

Language: Английский

Citations

4
Synthetically programmed antioxidant delivery by a domesticated skin commensal DOI Creative Commons
Guillermo Nevot, Javier Santos‐Moreno, Nil Campamà Sanz

et al.

Cell Systems, Journal Year: 2025, Volume and Issue: 16(2), P. 101169 - 101169

Published: Feb. 1, 2025

Bacteria represent a promising dynamic delivery system for the treatment of disease. In skin, relevant location Cutibacterium acnes within hair follicle makes this bacterium an attractive chassis dermal biotechnological applications. Here, we provide genetic toolbox engineering traditionally intractable bacterium, including basic gene expression tools, biocontainment strategies, markerless engineering, and transcriptional regulation. As proof concept, develop antioxidant-secreting strain capable reducing oxidative stress in UV model.

Language: Английский

Citations

2
Cutaneous Components Leading to Pruritus, Pain, and Neurosensitivity in Atopic Dermatitis: A Narrative Review DOI Creative Commons
Sonja Ständer, Thomas A. Luger, Brian Kim

et al.

Dermatology and Therapy, Journal Year: 2024, Volume and Issue: 14(1), P. 45 - 57

Published: Jan. 1, 2024

Atopic dermatitis (AD) is a chronic, relapsing immunoinflammatory skin condition characterized by sensations such as pruritis, pain, and neuronal hypersensitivity. The mechanisms underlying these are multifactorial involve complex crosstalk among several cutaneous components. This review explores the role components play in pathophysiology of atopic dermatitis. In intercellular spaces, sensory nerves interact with keratinocytes immune cells via myriad mediators receptors. These interactions generate action potentials that transmit pruritis pain signals from peripheral nervous system to brain. Keratinocytes, most abundant cell type epidermis, key effector cells, triggering neurons elicit inflammation. Filaggrin expression reduced dermatitis, causing weakened barrier elevated pH. Fibroblasts main dermis and, appear reduce keratinocyte differentiation, further weakening barrier. mast promote inflammation while dermal dendritic attenuate Inflammatory cytokines chemokines major AD pathogenesis. Type 2 responses typically 1 3 pain. increased pH contribute dysfunction dysbiosis microbiome, proliferation Staphyloccocus aureus. conclusion, understanding dynamic between could drive development therapies improve quality life for patients AD.

Language: Английский

Citations

12
An OX-Tra’Ordinary Tale: The Role of OX40 and OX40L in Atopic Dermatitis DOI Creative Commons
Kaviyon Sadrolashrafi, Lily Guo,

Robin Kikuchi

et al.

Cells, Journal Year: 2024, Volume and Issue: 13(7), P. 587 - 587

Published: March 28, 2024

The transmembrane glycoprotein OX40 receptor (OX40) and its ligand, OX40L, are instrumental modulators of the adaptive immune response in humans. functions as a costimulatory molecule that promotes T cell activation, differentiation, survival through ligation with OX40L. cells play an integral role pathogenesis several inflammatory skin conditions, including atopic dermatitis (AD). In particular, helper 2 (TH2) strongly contribute to AD via production cytokines associated type inflammation (e.g., IL-4, IL-5, IL-13, IL-31) lead barrier dysfunction pruritus. OX40-OX40L interaction also activation proliferation other populations TH1, TH22, TH17), patients have demonstrated higher levels expression on peripheral blood mononuclear than healthy controls. As such, pathway is potential target for treatment. Novel therapies targeting currently development, which promising safety efficacy results moderate-to-severe AD. Herein, we review function signaling pathway, their pathogenesis, emerging may offer insights into future management.

Language: Английский

Citations

9
Exploring the skin microbiome in atopic dermatitis pathogenesis and disease modification DOI
Claudia Hülpüsch, Robin Rohayem, Matthias Reiger

et al.

Journal of Allergy and Clinical Immunology, Journal Year: 2024, Volume and Issue: 154(1), P. 31 - 41

Published: May 16, 2024

Language: Английский

Citations

9
Controlling skin microbiome as a new bacteriotherapy for inflammatory skin diseases DOI Creative Commons
Yoshihiro Ito, Masayuki Amagai

Inflammation and Regeneration, Journal Year: 2022, Volume and Issue: 42(1)

Published: Sept. 1, 2022

Abstract The skin serves as the interface between human body and environment interacts with microbial community. microbiota consists of microorganisms, such bacteria, fungi, mites, viruses, they fluctuate depending on microenvironment defined by anatomical location physiological function. balance interactions host plays a pivotal role in orchestration homeostasis; however, disturbance due to an alteration communities, namely, dysbiosis, leads various disorders. Recent developments sequencing technology have provided new insights into structure function communities. Based high-throughput analysis, growing evidence indicates that treatment using live termed bacteriotherapy, is feasible therapeutic option for cutaneous diseases caused dysbiosis. In particular, administration specific bacterial strains has been investigated exclusionary strategy against pathogens associated chronic disorders, whereas safety, efficacy, sustainability this approach isolated bacteria need be further explored. review, we summarize our current understanding microbiota, well strategies characterized inflammatory diseases. ecosystem formed consortium still largely unexplored; advances at strain level will lead development methods.

Language: Английский

Citations

37
Microbiome and Allergy: New Insights and Perspectives DOI Open Access
Elisa Zubeldia‐Varela, Tomás Clive Barker‐Tejeda,

D Obeso

et al.

Journal of Investigational Allergology and Clinical Immunology, Journal Year: 2022, Volume and Issue: 32(5), P. 327 - 344

Published: Oct. 10, 2022

The role of the microbiome in molecular mechanisms underlying allergy has become highly relevant recent years. Studies are increasingly suggesting that altered composition microbiota, or dysbiosis, may result local and systemic alteration immune response to specific allergens. In this regard, a link been established between lung microbiota respiratory allergy, skin atopic dermatitis, gut food allergy. human is dynamic depends on host-associated factors such as diet, diseases, lifestyle. Omics techniques choice for analysis understanding microbiota. Microbiota have advanced considerably decades, need multiple approaches explore comprehend multifactorial including increased. Thus, more studies proposing intervention review, we present latest advances with respect literature, focusing intestinal, cutaneous, We discuss relationship system, emphasis allergic diseases. Finally, main technologies study interventions targeting prevention

Language: Английский

Citations

35