Advances in sperm cryopreservation in farm animals: Cattle, horse, pig and sheep

Animal Reproduction Science, Journal Year: 2021, Volume and Issue: 246, P. 106904 - 106904

Published: Dec. 3, 2021

Sperm cryopreservation is one of the most important procedures in development biotechnologies for assisted reproduction. In some farm animals, use cryopreserved sperm has so many benefits which relevance become more evident recent decades. Values post-thaw quality, however, are variable among species and within individuals same species. There no standardized methodology each stages procedure (andrological examination, semen collection, dilution, centrifugation, resuspension pellet with freezing medium, packaging, evaluation), also contributes to differences studies. Cryotolerance markers seminal plasma (SP) have been evaluated prediction ejaculate freezability. addition, previous research, there a focus on supplementing media different substances, such as enzymatic non-enzymatic antioxidants. studies, inclusion these substances led improved quality fertilizing capacity result minimizing adverse effects structure function. Another approach cryoprotectants. The aim this review article provide an update animals. main detrimental described, including negative repercussion reproductive performance. Furthermore, potential molecular biomarkers predict cryotolerance discussed, well addition that can mitigate harmful impact thawing sperm.

Language: Английский

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Boar Sperm Motility Assessment Using Computer-Assisted Sperm Analysis: Current Practices, Limitations, and Methodological Challenges

Animals, Journal Year: 2025, Volume and Issue: 15(3), P. 305 - 305

Published: Jan. 22, 2025

Spermatozoa are highly specialized male cells that characterized by a unique ability to move, which is critical factor for successful fertilization. The relative simplicity of motility assessment, especially in livestock, has made it widely used parameter evaluating ejaculate quality or cryopreserved semen the clinical field, and an advanced tool reproductive physiology toxicology research. Technological advances image analysis computational methods have substantially increased its accuracy through use computer-assisted sperm (CASA) minimize subjective bias assessments. Nevertheless, this more objective method still presents some significant challenges, including variability sample preparation, imaging conditions, analytical parameters. These issues contribute inconsistency impair reproducibility comparability data between laboratories. implementation standardized protocols, combined with comprehensive training rigorous evaluation, can serve mitigate emerging inconsistencies. In addition, vitro conditions under CASA analyses performed often differ significantly from natural environment female tract vivo. This review discusses methodologies, issues, limitations using CASA, particular focus on boar as important agricultural biomedical model species system used.

Language: Английский

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Sperm Motility Evaluation in Stallion Fresh, Cooled and Frozen Semen Using a Portable Computer‐Assisted Sperm Analysis System

Reproduction in Domestic Animals, Journal Year: 2025, Volume and Issue: 60(3)

Published: March 1, 2025

ABSTRACT Semen analysis is an important laboratory diagnostic test for stallions. Evaluation of sperm motility integral to basic semen and results are breeding management clinical practice. Computer‐assisted (CASA) allows objective evaluation increases analytical precision. The the present study was validate a portable CASA system (AndroScope). Fresh/cooled samples (33 ejaculates, 18 stallions) frozen (40 ejaculates one epididymal flush, 27 were evaluated using AndroScope IVOS II (‘gold standard’). Positive associations (R 2 = 0.31 0.83, p < 0.0001) between systems observed all estimates. percentage motile lower ( with than (mean difference: −4.2%), but progressively normal did not differ two systems. Systemic differences kinetic Sperm velocities, ALH, BCF AndroScope, observations likely associated slower video acquisition frame rate used by this when compared (45 Hz vs. 60 Hz, respectively). In conclusion, produced precise parameters. ability identify track sperm, analyse single kinematics seemed similar that ‘gold standard’ validation. must be taken into consideration evaluating kinetics setting thresholds classification different subpopulations.

Language: Английский

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A New Approach of Sperm Motility Subpopulation Structure in Donkey and Horse

Frontiers in Veterinary Science, Journal Year: 2021, Volume and Issue: 8

Published: May 25, 2021

This study aimed to characterize the sperm kinematic values with high frames per second, define subpopulation structure of a horse and donkey compare them. A total 57 fresh semen ejaculates (26 Spanish 16 Arabian breeds 10 donkeys) were collected subsequently analyzed for parameters using Computer-aided motility analysis ISAS®v1.2 system Spermtrack® 10-μm depth counting chamber. Sequences recorded at 250 eight automatically evaluated. All showed significant differences between breeds. evaluated excellent characteristics, significantly higher all donkeys compared horses except beat-cross frequency. Donkey was faster linear than horse. Regarding differences, had average path velocity, curvilinear frequency rapid, but Arab more linear. The principal component three subpopulations in ejaculate different characteristic dominant both straight, beat (38.2 41.7%, respectively), whereas lowest slowest non-linear sperms. This, plus slight distribution these horses, found. In conclusion, permitted have new interpretation motile species breed differences. More so, future works on spermatozoa should take into account that may interfere alter real performed.

Language: Английский

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The future of equine semen analysis

Reproduction Fertility and Development, Journal Year: 2024, Volume and Issue: 36(6)

Published: March 11, 2024

We are currently experiencing a period of rapid advancement in various areas science and technology. The integration high throughput 'omics' techniques with advanced biostatistics, the help artificial intelligence, is significantly impacting our understanding sperm biology. These advances will have an appreciable impact on practice reproductive medicine horses. This article provides brief overview recent field spermatology how they changing assessment quality. written from authors' perspective, using stallion as model. aim to portray changes occurring motility kinematics, flow cytometry, implementation technologies, use intelligence/self-learning data analysis. also briefly discuss some can be readily available practitioner, through 'on-farm' devices telemedicine.

Language: Английский

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El análisis seminal en la agricultura de precisión en el siglo XXI

Agronomía Mesoamericana, Journal Year: 2023, Volume and Issue: unknown, P. 51957 - 51957

Published: Jan. 30, 2023

Introducción. La reproducción asistida de animales tiene su origen en la domesticación las especies ganaderas útiles al ser humano. Los consiguientes procesos selección artificial permitieron desarrollar técnicas biotecnológicas que contribuyeron a mejora capacidades producción animal. Objetivo. Contextualizar el análisis seminal agricultura precisión siglo XXI. Desarrollo. visualización espermatozoides microscopio puede considerarse como primer paso advenimiento biotecnología y base para desarrollo asistida. Con perfeccionamiento los métodos microscopía, se logró caracterizar gametos masculinos, lo significó un avance significativo tecnología inseminación artificial. El punto inflexión marcado por conservación espermática, implicó cambio sustantivo estas tecnologías ganaderas, ya sea con semen crio preservado o refrigerado. Estos son alto valor caso amenazadas, dado pueden crear bancos germoplasma propósitos genética rescate riesgo extinción. ha desarrollado forma paralela asistida, hoy considera una técnica relevante animal, perfeccionado mediante ciencia tecnología, física óptica computación. Conclusión. tenido paradigma desechar obsoletas evaluación subjetiva calidad adoptar objetivos del semen, grandes volúmenes datos variables movilidad, cinéticas, morfométricas, morfológicas fragmentación ADN, permiten mejor eyaculados reproductores centros

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Optimization of human semen analysis using CASA-Mot technology

Systems Biology in Reproductive Medicine, Journal Year: 2023, Volume and Issue: 69(2), P. 166 - 174

Published: Feb. 20, 2023

The purpose of this study is to investigate the optimal framerate (FR) and use different counting chambers for improving CASA-Mot technology in Andrology. Images were captured at 500 fps, then segmented analyzed several ranges FRs (from 25 250) define asymptotic point that as an FR. This work was replicated using based capillarity (disposable) or drop displacement (reusable) their effects on motility results kinematic values samples under experimental conditions. α value (asymptote corresponding FRo) exponential curve 150.23 a VCL 130.58 mm/s, far from 98.89 mm/s 50 fps (the highest FR used by most current systems). Our have shown that, when reusable chambers, type depth influence. In addition, obtained depending area image inside chamber types. To reliable human sperm studies, almost 150 should be capturing analyzing differences between considered sampling areas, obtain representative whole sample.

Language: Английский

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Relationship between frame rates and subpopulation structure of bovine sperm divided by their motility analyzed by a computer‐assisted sperm analysis system

Animal Science Journal, Journal Year: 2022, Volume and Issue: 93(1)

Published: Jan. 1, 2022

The authors investigated the relationship between frame rates and subpopulation structure of bovine sperm divided by their motility analyzed a computer-assisted analysis (CASA). Kinematic parameters incubated in Brackett & Oliphant medium with without calcium ionophore for 0, 1, 5, 10, 15, 20, 25, 30 min were evaluated CASA at 150 frames per second (fps) cluster analysis. Then, we converted data fps to 75, 50, structures different each discriminant As results, was affected rate. Sperm into six clusters 150, 50 fps; on other hand, there five fps. Straight-line velocity similar all rates. However, as rate became higher, curvilinear beat cross frequency head increased significantly, whereas lateral displacement decreased significantly. In conclusion, higher is recommended capture trajectory accurately present study.

Language: Английский

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Effects of Media and Frame Rates on Hyperactivated Bovine Sperm Motility and an Analysis of Sperm Motility Subpopulation Structures in Sex-Sorted and Non-Sorted Semen

Published: April 5, 2021

We attempted to establish an objective method accurately evaluate the motility of bull sperm and examined effects media for suspensions frame rates on data computer-assisted analysis (CASA). Sperm incubated in Brackett Oliphant medium (BO) more clearly showed hyperactivation-like than those synthetic oviductal fluid. images captured at 150 frames per second (fps) a trajectory that was closer real pathway other (30, 50, 75 fps). then characteristics sex-sorted non-sorted semen using cluster followed by discriminant BO fps. The results indicated contained with as main subpopulation immediately after thawing this decreased 2-h incubation. had progressive maintained during In conclusion, usage capturing fps CASA were appropriate evaluating bovine motility. A from motile has ability describe differences structures subpopulations.

Language: Английский

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