Stored and Frozen Semen in Swine

Published: Jan. 1, 2025

Fertility after photodynamic inactivation of bacteria in extended boar semen

Frontiers in Microbiology, Journal Year: 2024, Volume and Issue: 15

Published: Aug. 7, 2024

Antimicrobial resistance is an increasing challenge in semen preservation of breeding animals, especially the porcine species. Bacteria are a natural component semen, and their growth should be inhibited to protect sperm fertilizing capacity female’s health. In pig breeding, where routinely stored at 17°C liquid state, alternatives conventional antibiotics urgently needed. Photodynamic inactivation (PDI) bacteria well-established tool medicine food industry but this technology has not been widely adopted preservation. The specific setting selectively inactivate while maintaining integrity functionality. aim study was test principle PDI boar using photosensitizer 5,10,15,20-tetrakis(N-methyl-4-pyridyl)-21H,23H-porphine (TMPyP) white light LED-setup. first step, photophysical experiments comprising singlet oxygen phosphorescence kinetics TMPyP determination triplet time revealed sufficiently high production reactive Androstar Premium extender, whereas seminal plasma acted as strong quencher. vitro with extended showed that established protocol preserves motility, membrane integrity, DNA mitochondrial activity efficiently reducing below detection limit. A proof-of-concept insemination confirmed vivo fertility after photodynamic treatment. conclusion, approach, innovative controls maintains fertility. This could promising contribution One Health concept potential reduce antimicrobial animal husbandry.

Language: Английский

Boar semen microbiome: insights and potential implications

Animal Reproduction Science, Journal Year: 2024, Volume and Issue: 272, P. 107647 - 107647

Published: Nov. 19, 2024

Language: Английский