A novel SARS-CoV-2-derived infectious vector system DOI Creative Commons

Ghada Elfayres,

Yong Xiao,

Qinghua Pan

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: Dec. 16, 2024

Abstract Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is the causative agent of COVID-19. The development antiviral drugs for COVID-19 has been hampered by requirement a biosafety level 3 (BSL3) laboratory experiments related to SARS-CoV-2, and lack easy precise methods quantification infection. Here, we developed SARS-CoV-2 viral vector composed all four structural proteins constitutively expressed in lentivirally transduced cells, combined with an RNA replicon deleted protein genes S, M E, expressing luciferase-GFP fusion protein. We show that, after concentrating stocks ultracentrifugation, able infect two human cell lines receptors ACE2 TMPRSS2. Both luciferase activity GFP fluorescence were detected, transduction was remdesivir-sensitive. also that this inhibited three type I interferons (IFN-I) subtypes. Although improvements are needed increase infectious titers, system may prove useful drug screening SARS-CoV-2-related investigations.

Language: Английский

Anti-SARS-CoV-2 and anticancer properties of triptolide and its derived carbonized nanomaterials DOI Creative Commons

Rubina Kousar,

Tahira Akhtar,

Chin‐Jung Lin

et al.

Cancer Letters, Journal Year: 2025, Volume and Issue: unknown, P. 217677 - 217677

Published: March 1, 2025

Language: Английский

Citations

0
Developing Zika virus-transduced hACE2 expression models for severe acute respiratory syndrome coronavirus 2 infection in vitro and in vivo DOI

Joh-Sin Wu,

Ju-Ying Kan,

Young-Sheng Chang

et al.

Journal of Virological Methods, Journal Year: 2025, Volume and Issue: 336, P. 115166 - 115166

Published: April 14, 2025

Language: Английский

Citations

0
Design and Application of Biosafe Coronavirus Engineering Systems without Virulence DOI Creative Commons
Guoqiang Wu, Qiaoyu Li, Junbiao Dai

et al.

Viruses, Journal Year: 2024, Volume and Issue: 16(5), P. 659 - 659

Published: April 24, 2024

In the last twenty years, three deadly zoonotic coronaviruses (CoVs)—namely, severe acute respiratory syndrome coronavirus (SARS-CoV), Middle East (MERS-CoV), and SARS-CoV-2—have emerged. They are considered highly pathogenic for humans, particularly SARS-CoV-2, which caused 2019 CoV disease pandemic (COVID-19), endangering lives health of people globally causing unpredictable economic losses. Experiments on wild-type viruses require biosafety level 3 or 4 laboratories (BSL-3 BSL-4), significantly hinders basic virological research. Therefore, development various biosafe systems without virulence is urgently needed to meet requirements different research fields, such as antiviral vaccine evaluation. This review aimed comprehensively summarize engineering systems. These combine foundations with synthetic genomics techniques, enabling efficient tools attenuated non-virulent vaccines, screening drugs, investigation mechanisms novel microorganisms.

Language: Английский

Citations

1
Glycyrrhizic acid conjugates with amino acid methyl esters target the main protease, exhibiting antiviral activity against wild-type and nirmatrelvir-resistant SARS-CoV-2 variants DOI

Uyen Nguyen Phuong Le,

Yu-Jen Chang, Chih‐Hao Lu

et al.

Antiviral Research, Journal Year: 2024, Volume and Issue: 227, P. 105920 - 105920

Published: May 29, 2024

Language: Английский

Citations

1
RNA replication-independent, DNA linearization-dependent expression of reporter genes from a SARS-CoV-2 replicon-encoding DNA in human cells DOI Creative Commons

Ronja Friedhoff,

Ghada Elfayres,

Natacha Mérindol

et al.

PLoS ONE, Journal Year: 2024, Volume and Issue: 19(8), P. e0300491 - e0300491

Published: Aug. 16, 2024

Replicons, derived from RNA viruses, are genetic constructs retaining essential viral enzyme genes while lacking key structural protein genes. Upon introduction into cells, the carried by replicon expressed, and self-replicates, yet particle production does not take place. Typically, replicons transcribed in vitro then electroporated cells. However, it would be advantageous for to generated cells following DNA transfection instead of RNA. In this study, a bacterial artificial chromosome (BAC) encoding SARS-CoV-2 under control T7 promoter was transfected HEK293T engineered functionally express polymerase (T7 RNAP). BAC DNA, we observed low, but reproducible expression reporter proteins GFP luciferase replicon. Expression required linearization prior transfection. Moreover, occurred independently RNAP. Gene also insensitive remdesivir treatment, suggesting that did involve self-replication Similar results were obtained highly infection-permissive Calu-3 Strikingly, N boosted DNA. conclusion, large coronaviral led gene through an unidentified mechanism. These findings highlight novel pathway toward cDNA, offering valuable insights development methods DNA-based applications.

Language: Английский

Citations

1
Assessing the inhibitory effects of some secondary amines, thioureas and 1,3-dimethyluracil conjugates of (-)-cytisine and thermopsine on the RNA-dependent RNA polymerase of SARS-CoV-1 and SARS-CoV-2 DOI
Yeh Chen, Mann‐Jen Hour, Chen‐Sheng Lin

et al.

Bioorganic & Medicinal Chemistry Letters, Journal Year: 2024, Volume and Issue: 113, P. 129950 - 129950

Published: Sept. 7, 2024

Language: Английский

Citations

1
A novel SARS-CoV-2-derived infectious vector system DOI Creative Commons

Ghada Elfayres,

Yong Xiao,

Qinghua Pan

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: Dec. 16, 2024

Abstract Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is the causative agent of COVID-19. The development antiviral drugs for COVID-19 has been hampered by requirement a biosafety level 3 (BSL3) laboratory experiments related to SARS-CoV-2, and lack easy precise methods quantification infection. Here, we developed SARS-CoV-2 viral vector composed all four structural proteins constitutively expressed in lentivirally transduced cells, combined with an RNA replicon deleted protein genes S, M E, expressing luciferase-GFP fusion protein. We show that, after concentrating stocks ultracentrifugation, able infect two human cell lines receptors ACE2 TMPRSS2. Both luciferase activity GFP fluorescence were detected, transduction was remdesivir-sensitive. also that this inhibited three type I interferons (IFN-I) subtypes. Although improvements are needed increase infectious titers, system may prove useful drug screening SARS-CoV-2-related investigations.

Language: Английский

Citations

0