Both the scion and rootstock of grafted grapevines influence the rhizosphere and root endophyte microbiomes, but rootstocks have a greater impact

Environmental Microbiome, Journal Year: 2024, Volume and Issue: 19(1)

Published: April 23, 2024

Abstract Background Soil microorganisms play an extensive role in the biogeochemical cycles providing nutrients necessary for plant growth. Root-associated bacteria and fungi, originated from soil, are also known to influence host health. In response environmental stresses, roots exude specific molecules influencing composition functioning of rhizospheric root microbiomes. This is genotype-dependent affected by soil microbiological chemical properties. It essential unravel grapevine rootstock scion genotypes on this microbiome, investigate relationship with growth adaptation its environment. Here, predicted functions microbiome system were studied using metabarcoding ten scion-rootstock combinations, addition nutrition measurements. Results The genotype significantly influenced diversity structure bacterial fungal as well rhizosphere compartments when grafted same cultivar. Based β-diversity analyses, 1103P showed distinct communities compared five others (RGM, SO4, 41B, 3309 C Nemadex). was more variable depending community investigated compartment. Its contribution primarily observed measured fungi both compartments, arbuscular mycorrhizal (AMF) rhizosphere. Significant correlations established between microbial variables phenotype, mineral status petioles roots. Conclusion These results shed light capacity recruit different functional microorganisms, which affect Selecting rootstocks capable associating positive symbiotic tool that can facilitate move towards sustainable viticulture help cope constraints.

Language: Английский

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New insights into the structure, microbial diversity and ecology of yellow biofilms in a Paleolithic rock art cave (Pindal Cave, Asturias, Spain)

The Science of The Total Environment, Journal Year: 2023, Volume and Issue: 897, P. 165218 - 165218

Published: July 6, 2023

In the absence of sunlight, caves harbor a great diversity microbial colonies to extensive biofilms with different sizes and colors visible naked eye. One most widespread types biofilm are those yellow hues that can constitute serious problem for conservation cultural heritage in many caves, such as Pindal Cave (Asturias, Spain). This cave, declared World Heritage Site by UNESCO its Paleolithic parietal art, shows high degree development represents real threat painted engraved figures. study aims to: 1) identify structures characteristic taxa composing biofilms, 2) seek linked microbiome reservoir primarily contributing their growth; 3) seed light on driving vectors contribute formation determine subsequent proliferation spatial distribution. To achieve this goal, we used amplicon-based massive sequencing, combination other techniques microscopy, situ hybridization environmental monitoring, compare communities drip waters, cave sediments exterior soil. The results revealed related phylum Actinomycetota bacteria represented genera wb1-P19, Crossiella, Nitrospira, Arenimonas. Our findings suggest serve potential reservoirs colonization sites these develop into under favorable substrate conditions, particular affinity speleothems rugged-surfaced rocks found condensation-prone areas. presents an exhaustive which could be procedure identification similar design effective strategies valuable heritage.

Language: Английский

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Microbiota Ecosystem Services in Vineyards and Wine: A Review

Agronomy, Journal Year: 2024, Volume and Issue: 14(1), P. 131 - 131

Published: Jan. 4, 2024

The domestication of vines started in Asia 11,000 years ago, although it was not until the 19th century that oenology established as a scientific discipline thanks to research Louis Pasteur on role microorganisms wine fermentation. At present time, progression next-generation sequencing (NGS) technologies is helping facilitate identification microbial dynamics during winemaking. These advancements have aided winemakers gaining more comprehensive understanding microbiota fermentation process, which, turn, ultimately responsible for delivery provisioning (wine features and its production), regulating (such carbon storage by vineyards, regulation soil quality, biocontrol pests diseases) or cultural aesthetic values vineyard landscapes, scholarly enjoyment wine, sense belonging wine-growing regions) ecosystem services. To our knowledge, this first review state knowledge services sector, well possibility valuing them monetary terms operating logic chains, such those suggested SEEA-EA framework. This paper concludes with management practices may enhance value smart farming task.

Language: Английский

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Microbial dysbiosis in roots and rhizosphere of grapevines experiencing decline is associated with active metabolic functions

Frontiers in Plant Science, Journal Year: 2024, Volume and Issue: 15

Published: April 2, 2024

When grapevine decline, characterized by a premature decrease in vigor and yield sometimes plant death, cannot be explained pathological or physiological diseases, one may inquire whether the microbiological status of soil is responsible. Previous studies have shown that composition structure bacterial fungal microbial communities inter-row are affected areas displaying vine compared to with non-declining vines within same plot. A more comprehensive analysis was conducted such Although chemical parameters could not directly explain these differences, declining presented lower vigor, yield, berry quality, petiole mineral content than those vines. The microbiome root endosphere, rhizosphere, different horizons bulk were explored through enzymatic, metabolic diversity, metabarcoding both areas. Despite diversity richness symptomatic roots soil, higher activity enrichment potentially beneficial bacteria pathogenic fungi found area. Path modeling linked suggesting determinant role content. Furthermore, certain taxa correlated predicted pathways processes assessed Eco-Plates. These results unexpectedly revealed active profiles belowground compartments associated stressed vines, highlighting interest exploring functional microbiota plants, specifically under conditions.

Language: Английский

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Soil Microbial Communities and Wine Terroir: Research Gaps and Data Needs

Published: June 26, 2024

Microbes found in soil can have a significant impact on the taste and quality of wine, also referred to as wine terroir. To date, terroir has been thought be associated with physical chemical characteristics soil. However, there is fragmented understanding contribution vineyard microbes Additionally, vineyards play an important role carbon sequestration, since promotion healthy microbial communities directly greenhouse gas emissions atmosphere. We review 24 studies that explore their influence grapevine health, grape composition, quality. Studies spanning 2015 2018 laid foundation by exploring biogeography vineyards, management effects, reservoir function for grape-associated microbiota. On other hand, 2019 2023 appear more specific targeted approach, delving into relationships between metabolites, distribution at different depths, influences flavor composition. Next, we identify research gaps make recommendations future work. Specifically, most utilize sequencing (16S, 26S, ITS) which only reveals community Utilizing high-throughput omics approaches such shotgun (to infer function) transcriptomics (for actual vital determine mechanisms chemistry. Going forward, long-term effects practices climate change microbiology, trunk diseases, bacteriophages making would fruitful investigation. Overall, presented shed light importance microbiomes interactions grapevines shaping production. are still many aspects this complex ecosystem require further exploration support sustainable viticulture enhance

Language: Английский

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Grape Berry Morphology in Semi-Arid Climate of Tekirdağ: Evaluating the Effects of Environmental Factors and Stress Applications

Black Sea Journal of Agriculture, Journal Year: 2024, Volume and Issue: 7(2), P. 144 - 156

Published: Jan. 24, 2024

The growth and development of grapes are influenced by various biotic abiotic stresses. presence Vitis vinifera L. on Earth is threatened the increase in stresses due to global warming. On other hand, grape quality and, consequently, berry characteristics can also be negatively affected these stress factors. hypothesis this experiment determine effects applied five days before harvest berries live grapevines under field conditions. For purpose, for two years (2016 2017), Cabernet-Sauvignon Merlot varieties grafted onto SO4 rootstock at Te-Ha Corp. vineyard were used. In late pre-harvest period (five harvest), seven applications, including control, implemented. application methods included impact (1 minute with a plastic hammer 08:00 19:00), leaf removal (removing all leaves), injury (injuring leaves hitting stick), UV-C vibration Botrytis cinerea Pers ex. Fr (once). measurements features performed as follows, order: width-length (mm), bery volume (cm3), skin area (cm2/grain), area/berry flesh ratio (cm2/cm3), fresh-dry weight (g), 100 fresh density (g/cm3), % dry weight. As result, it was observed that treatments did not affect years, especially second year. Therefore, Shock action, UV-C, Vibration, Leaf injury, removal, improving found objectionable.

Language: Английский

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Rhizobacteria from vineyard and commercial mycorrhizal fungi induce synergistic microbiome shifts within grapevine root systems

Research Square (Research Square), Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 28, 2025

Background The addition of bacteria and arbuscular mycorrhizal fungi (AMF) is a strategy used to protect plants against disease improve their growth yield, known as biocontrol biostimulation, respectively. In viticulture, the plant promotion (PGP) potential endemic vineyard soil has been underexplored. Furthermore, most research about microbial biostimulants focuses on effect plant, but little how application modify root ecosystem, which may have an impact resistance. objectives this work were 1) identify present in soils with functional PGP traits, 2) test activity young grapevines, combination AMF, 3) assess communities inferred functions rhizosphere roots. Results Two hundred isolated from vineyards characterized for biochemical activities. efficient tested in vitro, both singly combination, Lepidium sativum grapevine plantlets. Pseudomonas species particularly increased vitro selected further testing, without two Glomus species, grapevines planted experiencing dysbiosis greenhouse setting. After five months growth, co-application rhizobacteria AMF significantly enhanced biomass abundance potentially beneficial bacterial genera roots, compared untreated conditions single inoculum treatments. Additionally, prevalence Botrytis cinerea, associated diseases, decreased endosphere. combined inoculation resulted more complex network higher metabolic functionality than Conclusions To our knowledge, first study examine apply strains derived same plot commercialized fungi. results show remodeling terms presence pathogens. observed synergistic indicates that it important consider effects individuals synthetic applied field.

Language: Английский

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The genotypes of the two components of grafted grapevines influence the rhizosphere and root endophyte microbiomes, with rootstocks having a greater impact

Research Square (Research Square), Journal Year: 2024, Volume and Issue: unknown

Published: Jan. 23, 2024

Abstract Background Soil microorganisms play an extensive role in the biogeochemical cycles providing nutrients necessary for plant growth. Root-associated bacteria and fungi, originated from soil, are also known to influence host health. In response environmental stresses, roots exude specific molecules influencing composition functioning of rhizospheric root microbiomes. This is genotype-dependent affected by soil microbiological chemical properties. It essential unravel grapevine rootstock scion genotypes on this microbiome, investigate relationship with growth adaptation its environment. Here, predicted functions microbiome system were studied using metabarcoding ten scion-rootstock combinations, addition nutrition measurements. Results The genotype significantly influenced diversity structure bacterial fungal as well rhizosphere compartments when grafted same cultivar. Based β-diversity analyses, 1103P showed distinct communities compared five others (RGM, SO4, 41B, 3309C Nemadex). was more variable depending community investigated compartment. Its contribution primarily observed measured fungi both compartments, arbuscular mycorrhizal (AMF) rhizosphere. Significant correlations established between microbial variables phenotype, mineral status petioles roots. Conclusion These results shed light capacity recruit different functional microorganisms, which affect Selecting rootstocks capable associating positive symbiotic tool that can facilitate move towards sustainable viticulture help cope constraints.

Language: Английский

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Grapevine Rootstock and Scion Genotypes’ Symbiosis with Soil Microbiome: A Machine Learning Revelation for Climate-Resilient Viticulture

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: Feb. 27, 2024

Abstract Given the impact of climate change on agriculture, development resilient crop cultivars is imperative. A healthy plant microbiota key to productivity, influencing nutrient absorption, disease resistance, and overall vigor. The genetic factors controlling assembly microbial communities are still unknown. Here we examine if Machine Learning can predict grapevine rootstock scion genotypes based soil microbiota, despite environmental variability. study utilized bacteriome datasets from 281 vineyards across 13 countries five continents, featuring 34 different Vitis vinifera grafted onto, often ambiguous, rootstocks. Random Forests, Adaptive Boost, Gradient Support Vector Machines, Gaussian Bernoulli Naïve Bayes, k-Nearest Neighbor, Neural Networks algorithms were employed continent, country, scion, cultivar, under two filtering criteria: retaining sparse classes, ensuring class diversity, excluding classes assessing model robustness against overfitting. Both criteria showed remarkable F1-weighted scores (>0.8) for all most algorithms. Moreover, successful genotype prediction microbiomes confirms that both parts shape microbiome. These insights pave way identifying genes use with breeding programs enhance productivity sustainability by improving plant-microbiota relationship.

Language: Английский

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Soil Microbial Communities and Wine Terroir: Research Gaps and Data Needs

Foods, Journal Year: 2024, Volume and Issue: 13(16), P. 2475 - 2475

Published: Aug. 6, 2024

Microbes found in soil can have a significant impact on the taste and quality of wine, also referred to as wine terroir. To date, terroir has been thought be associated with physical chemical characteristics soil. However, there is fragmented understanding contribution vineyard microbes Additionally, vineyards play an important role carbon sequestration since promotion healthy microbial communities directly impacts greenhouse gas emissions atmosphere. We review 24 studies that explore their influence grapevine health, grape composition, quality. Studies spanning 2015 2018 laid foundation by exploring biogeography vineyards, management effects, reservoir function for grape-associated microbiota. On other hand, 2019 2023 appear more specific targeted approach, delving into relationships between metabolites, distribution at different depths, influences flavor composition. Next, we identify research gaps make recommendations future work. Specifically, most utilize sequencing (16S, 26S, ITS), which only reveals community Utilizing high-throughput omics approaches such shotgun (to infer function) transcriptomics (for actual vital determining mechanisms chemistry. Going forward, long-term effects practices climate change microbiology, trunk diseases, bacteriophages wine-making would fruitful investigation. Overall, presented shed light importance microbiomes interactions grapevines shaping production. are still many aspects this complex ecosystem require further exploration support sustainable viticulture enhance

Language: Английский

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