Integrate Analysis of Eyestalk Proteome and Metabolome in Precocious and Formal Juvenile Female Eriocheir sinensis DOI Creative Commons
Tingshuang Pan, Min Yang, Tong Li

et al.

Fishes, Journal Year: 2024, Volume and Issue: 9(11), P. 468 - 468

Published: Nov. 18, 2024

The Chinese mitten crab (Eriocheir sinensis) is an economically important crustacean. With the development of E. sisnensis industry, precocity has become a significant challenge in juvenile culturing. In this study, eyestalks female sinensis from precocious (PE) and normal (NE) groups were used for proteome metabolome analyses. total, 731 up-regulated 657 down-regulated differentially expressed proteins (DEPs) identified PE NE groups. addition, 110 metabolites (DMs) 256 group. An integrated analysis showed 5667 correlations between 109 common pathways metabolome. mostly associated with mechanistic target rapamycin (mTOR) pathway, longevity regulation, autophagy, pyrimidine purine metabolism pathways. primarily enriched amino acid lipid metabolisms. These results demonstrated differences at two omics levels will be useful industry.

Language: Английский

Sperm acrosomal released proteome reveals MDH and VDAC3 from mitochondria are involved in acrosome formation during spermatogenesis in Eriocheir sinensis DOI
Chao Li, Ruifang Yu, Huan Liu

et al.

Gene, Journal Year: 2023, Volume and Issue: 887, P. 147784 - 147784

Published: Sept. 7, 2023

Language: Английский

Citations

5
Comparative Proteomics Elucidates the Potential Mechanism of Sperm Capacitation of Chinese Mitten Crabs (Eriocheir sinensis) DOI
Xiaodong Cui,

Xueqian Cai,

Fenghao Zhang

et al.

Journal of Proteome Research, Journal Year: 2024, Volume and Issue: 23(5), P. 1603 - 1614

Published: April 1, 2024

Sperm capacitation is broadly defined as a suite of biochemical and biophysical changes resulting from the acquisition fertilization ability. To gain insights into regulation mechanism crustacean sperm capacitation, 4D label-free quantitative proteomics was first applied to analyze in Eriocheir sinensis under three sequential physiological conditions: seminal vesicles (X2), hatched with receptacle content (X3), incubated egg water (X5). In total, 1536 proteins were identified, among which 880 quantified, 82 224 significantly altered after incubation contents water. Most differentially expressed attributed biological processes by Gene Ontology annotation analysis. As fundamental bioenergetic metabolism sperm, oxidative phosphorylation, glycolysis, pentose phosphate pathway presented significant treatment contents, indicating intensive for receptacle. Additionally, also increased oxidation level whereas enhancement abundance superoxide dismutase, peroxiredoxin 1, glutathione S-transferase improved resistance against oxidation. These results provided new perspective reproduction studies crustaceans.

Language: Английский

Citations

1
One-Year-Old Precocious Chinese Mitten Crab Identification Algorithm Based on Task Alignment DOI Creative Commons
Hao Gu,

Dongmei Gan,

Ming Chen

et al.

Animals, Journal Year: 2024, Volume and Issue: 14(14), P. 2128 - 2128

Published: July 21, 2024

The cultivation of the Chinese mitten crab (

Language: Английский

Citations

1
The Dmrt1 gene: Identification and functional analysis in testicular development and spermatogenesis in the freshwater prawn, Macrobrachium nipponense DOI

Haifu Wan,

Lei Yu, Xiaoyu Zhang

et al.

Aquaculture, Journal Year: 2024, Volume and Issue: 596, P. 741856 - 741856

Published: Nov. 8, 2024

Language: Английский

Citations

0
Integrate Analysis of Eyestalk Proteome and Metabolome in Precocious and Formal Juvenile Female Eriocheir sinensis DOI Creative Commons
Tingshuang Pan, Min Yang, Tong Li

et al.

Fishes, Journal Year: 2024, Volume and Issue: 9(11), P. 468 - 468

Published: Nov. 18, 2024

The Chinese mitten crab (Eriocheir sinensis) is an economically important crustacean. With the development of E. sisnensis industry, precocity has become a significant challenge in juvenile culturing. In this study, eyestalks female sinensis from precocious (PE) and normal (NE) groups were used for proteome metabolome analyses. total, 731 up-regulated 657 down-regulated differentially expressed proteins (DEPs) identified PE NE groups. addition, 110 metabolites (DMs) 256 group. An integrated analysis showed 5667 correlations between 109 common pathways metabolome. mostly associated with mechanistic target rapamycin (mTOR) pathway, longevity regulation, autophagy, pyrimidine purine metabolism pathways. primarily enriched amino acid lipid metabolisms. These results demonstrated differences at two omics levels will be useful industry.

Language: Английский

Citations

0