Genome-wide characterization of the AlkB homolog (ALKBH) gene family in Litopenaeus vannamei identifies LvALKBH1 and LvALKBH8 as promising crustacean m6A demethylases involved in molting regulation and ammonia stress response

International Journal of Biological Macromolecules, Journal Year: 2025, Volume and Issue: unknown, P. 140425 - 140425

Published: Jan. 1, 2025

Language: Английский

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Epigenetic regulation of physiological resilience to ammonia nitrogen stress in the Pacific whiteleg shrimp Penaeus vannamei: Evidence from genome-wide DNA methylation dynamics

Comparative Biochemistry and Physiology Part D Genomics and Proteomics, Journal Year: 2025, Volume and Issue: unknown, P. 101510 - 101510

Published: April 1, 2025

Language: Английский

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Integrated m6A RNA methylation and transcriptomic analysis of Apostichopus japonicus under combined high-temperature and hypoxia stress

BMC Genomics, Journal Year: 2025, Volume and Issue: 26(1)

Published: April 10, 2025

Global climate change has significantly increased environmental stress in marine ecosystems, with rising sea surface temperatures and declining dissolved oxygen (DO) levels. These stressors pose critical challenges to aquaculture, particularly for Apostichopus japonicus, an economically significant species China. A. japonicus is highly sensitive combined high-temperature hypoxia stress, which disrupts physiological processes, suppresses immune responses, increases mortality. While epigenetic mechanisms such as N6-methyladenosine (m6A) RNA modifications are known regulate adaptation, their role under dual remains poorly understood. This study integrates m6A methylation sequencing (MeRIP-seq) transcriptomic analysis (RNA-seq) investigate molecular responses (32 °C) (DO = 2 mg/L). Results show that approximately 90% of genes had 1-3 peaks, single peaks being the most frequent (∼ 60%). Genes exhibited varying expression levels, some showing higher expression, suggesting a complex relationship between stress-responsive gene expression. GO KEGG enrichment analyses revealed m6A-modified pathways associated oxidative protein homeostasis, energy metabolism, PI3K-Akt MAPK signaling pathways. Key genes, including HSP70, NOX5, SLC7A11, dynamic changes, highlighting roles redox homeostasis cellular resilience. Comparative across experimental groups distinct hypoxia, combination, inducing more pronounced changes In this study, we explored central regulatory response hypoxia. The findings modification regulates key allowing effectively adapt harsh conditions. not only provides important new perspective on recovery mechanism invertebrates face but it also theoretical support aquaculture practice, assisting development stress-resistant systems deal severe posed by global change.

Language: Английский

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Epigenetic Modulations for Prevention of Infectious Diseases in Shrimp Aquaculture

Genes, Journal Year: 2023, Volume and Issue: 14(9), P. 1682 - 1682

Published: Aug. 25, 2023

Aquaculture assumes a pivotal role in meeting the escalating global food demand, and shrimp farming, particular, holds significant economy security, providing rich source of nutrients for human consumption. Nonetheless, industry faces formidable challenges, primarily attributed to disease outbreaks diminishing efficacy conventional management approaches, such as antibiotic usage. Consequently, there is an urgent imperative explore alternative strategies ensure sustainability industry. In this context, field epigenetics emerges promising avenue combating infectious diseases aquaculture. Epigenetic modulations entail chemical alterations DNA proteins, orchestrating gene expression patterns without modifying underlying sequence through methylation, histone modifications, non-coding RNA molecules. Utilizing epigenetic mechanisms presents opportunity enhance immune bolster resistance shrimp, thereby contributing optimizing health productivity. Additionally, concept inheritability marine animals immense potential future farming To end, comprehensive review thoroughly explores dynamics aquaculture, with particular emphasis on its management. It conveys significance harnessing advantageous changes long-term viability while deliberating consequences these interventions. Overall, appraisal highlights trajectory applications, propelling toward strengthening

Language: Английский

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Dynamic N6-methyladenosine RNA methylation landscapes reveal epi-transcriptomic modulation induced by ammonia nitrogen exposure in the Pacific whiteleg shrimp Litopenaeus vannamei

Journal of Hazardous Materials, Journal Year: 2023, Volume and Issue: 458, P. 131996 - 131996

Published: July 5, 2023

Language: Английский

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Nanopore direct RNA sequencing deciphering the m6A epitranscriptome and its role in the innate immune response of the mussel Mytilus coruscus:a prospective study of RNA modification in marine molluscs

Aquaculture, Journal Year: 2024, Volume and Issue: unknown, P. 742040 - 742040

Published: Dec. 1, 2024

Language: Английский

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The role of methyltransferase-like 3 (METTL3) in immune response modulation in bivalve (Mytilus coruscus) during bacterial infection.

Fish & Shellfish Immunology, Journal Year: 2024, Volume and Issue: unknown, P. 110094 - 110094

Published: Dec. 1, 2024

Language: Английский

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