Effect of titanium dioxide nanoparticle (TiO2‐NP) exposure in a novel Amur sturgeon Acipenser schrenckii hepatocyte cell line DOI
Zhou Zhou, Ting Liu,

Tianxun Luo

et al.

Journal of Fish Biology, Journal Year: 2024, Volume and Issue: 105(3), P. 894 - 906

Published: June 22, 2024

Abstract In vitro cell culture is crucial for predicting the toxicity of titanium dioxide nanoparticle (TiO 2 ‐NP). However, assessing TiO ‐NPs in sturgeon remains difficult given lack sufficient lines. We established and characterized first hepatocyte line from Acipenser schrenckii liver tissue (ASL). This ASL proliferated well Dulbecco's modified Eagle's medium at 25°C 10% fetal bovine serum. cells with a chromosome number 244 were successfully transfected pEGFP‐N3 plasmid. The line's origin was verified as A. through mitochondrial cytochrome C oxidase I 16S ribosomal RNA (rRNA) sequencing. Using an model, we found that ‐NP exposure decreased viability promoted damage (96‐h LC 50 = 331.8 μg mL −1 ). Increased reactive oxygen species malondialdehyde levels suggested oxidative stress under exposure. also observed dysregulation aspartate aminotransferase alanine levels. By detecting calcium ions membrane potential indicators, apoptotic pathway induced by endoplasmic reticulum played major role low concentrations ‐NP‐induced stress. Both mitochondria‐mediated apoptosis increasing concentrations. conclusion, this study useful model toxicological studies fish.

Language: Английский

The effect of low concentrations of selected metal ions (Al, Ag, Cu, Mn) on the properties of ZnO nanoparticles prepared by the co-precipitation technique DOI
Rabie Amari, Smail Terchi,

Djamel Allali

et al.

Materials Letters, Journal Year: 2025, Volume and Issue: unknown, P. 138677 - 138677

Published: May 1, 2025

Language: Английский

Citations

0
Transcriptomic analyses of Pinctada fucata martensii responses under stress of titanium dioxide nanoparticles DOI Creative Commons
Fengfeng Li,

Jiaen Liu,

Zixin Gao

et al.

Frontiers in Marine Science, Journal Year: 2024, Volume and Issue: 11

Published: Aug. 23, 2024

Titanium dioxide nanoparticles (TiO 2 -NPs) released into the environment is becoming more prevalent due to their increased usage, marine TiO -NPs contamination escalating concerns in coastal areas. To understand potential impact of on transcript changes pearl oyster ( Pinctada fucata martensii ), transcriptome analysis gill tissues oysters was conducted after 14-day exposure and 7-day brief recovery. A total 911 differentially expressed genes (DEGs) were identified between control group (TC) experimental (TE) exposed -NPs. Gene ontology (GO) analyses DEGs demonstrated substantial enrichments functions related “hydrolase activity”, “oxidoreductase “DNA integration”. The Kyoto Encyclopedia Genes Genomes (KEGG) pathways indicated enrichment several pathways, including “ubiquitin-mediated protein hydrolysis”, “ECM-receptor interactions”, “NOD-like receptor signaling pathway”, “Toll-like receptor”, “FOXO pathway”. This suggests that intensifies oxidative stress apoptosis pearls oysters, leading negative effects such as disrupted homeostasis, decreased biomineralization activity, reduced neuronal excitability, weakened immune response, cellular metabolism. Transcriptome 844 TE recovery (TR), which underwent a period. GO integration”, “obsolete oxidation-reduction process”, “proteolysis”. KEGG “lysine degradation”, “glycine, serine, threonine metabolism”, findings although showed only slight relief 7 days recovery, they continued experience from -NP exposure. Our shed light complex responses offer valuable theoretical insights toxicological oysters.

Language: Английский

Citations

3
Effect of titanium dioxide nanoparticle (TiO2‐NP) exposure in a novel Amur sturgeon Acipenser schrenckii hepatocyte cell line DOI
Zhou Zhou, Ting Liu,

Tianxun Luo

et al.

Journal of Fish Biology, Journal Year: 2024, Volume and Issue: 105(3), P. 894 - 906

Published: June 22, 2024

Abstract In vitro cell culture is crucial for predicting the toxicity of titanium dioxide nanoparticle (TiO 2 ‐NP). However, assessing TiO ‐NPs in sturgeon remains difficult given lack sufficient lines. We established and characterized first hepatocyte line from Acipenser schrenckii liver tissue (ASL). This ASL proliferated well Dulbecco's modified Eagle's medium at 25°C 10% fetal bovine serum. cells with a chromosome number 244 were successfully transfected pEGFP‐N3 plasmid. The line's origin was verified as A. through mitochondrial cytochrome C oxidase I 16S ribosomal RNA (rRNA) sequencing. Using an model, we found that ‐NP exposure decreased viability promoted damage (96‐h LC 50 = 331.8 μg mL −1 ). Increased reactive oxygen species malondialdehyde levels suggested oxidative stress under exposure. also observed dysregulation aspartate aminotransferase alanine levels. By detecting calcium ions membrane potential indicators, apoptotic pathway induced by endoplasmic reticulum played major role low concentrations ‐NP‐induced stress. Both mitochondria‐mediated apoptosis increasing concentrations. conclusion, this study useful model toxicological studies fish.

Language: Английский

Citations

1