Decapod iridescent virus 1 replication dynamics in Macrobrachium rosenbergii at different water temperatures and the host immune response

Aquaculture, Journal Year: 2025, Volume and Issue: unknown, P. 742113 - 742113

Published: Jan. 1, 2025

Language: Английский

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The effects of short neuropeptide F on ovarian maturation and spawning in female giant freshwater prawn, Macrobrachium rosenbergii, and associated regulatory mechanisms

Aquaculture, Journal Year: 2023, Volume and Issue: 569, P. 739361 - 739361

Published: Feb. 15, 2023

Language: Английский

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Optimal Reference Genes for Gene Expression Analysis of Overmating Stress-Induced Aging and Natural Aging in Male Macrobrachium rosenbergii

International Journal of Molecular Sciences, Journal Year: 2025, Volume and Issue: 26(8), P. 3465 - 3465

Published: April 8, 2025

Functional gene expression is closely linked to an organism's physiology and can be quantified using Real-Time Quantitative Reverse Transcription PCR (RT-qPCR). However, the stability of reference not absolute, which may impact accuracy RT-qPCR results. In this study, we evaluated suitability nine genes including receptor for activated protein kinase c1 (rack1), ribosomal L6 (rpl6), L9 (rpl9), S2 (rps2), S18 (rps18), lateral stalk subunit P0 (rplp0), eukaryotic translation elongation factor 1β (eef1b), initiation 4a (eif4a), 5a (eif5a) analyzed from RNA sequencing (RNA-Seq) data in addition three 1α (eef1a), β-actin (actb), glyceraldehyde 3-phosphate dehydrogenase (gapdh) selected literature obtain best internal controls analysis M. rosenbergii under overmating stress natural aging. RefFinder was used comprehensively evaluate candidate genes. The initial results showed that (eif5a, rps18, rplp0) RNA-Seq had relatively stable levels, were more than those commonly Eif5a rps18 combination Further indicated eif5a might study rosenbergii.

Language: Английский

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Cryopreservation of spermatogonia of the giant freshwater prawn, Macrobrachium rosenbergii, using slow and ultra-rapid freezing

Cryobiology, Journal Year: 2025, Volume and Issue: 119, P. 105242 - 105242

Published: April 15, 2025

The giant freshwater prawn, Macrobrachium rosenbergii, is an important species that widely raised in tropical and subtropical regions. To develop techniques for preserving valuable traits this species, we have devised protocols cryopreserving spermatogonia-progenitor cells give rise to spermatozoa through spermatogenesis-using slow freezing ultra-rapid techniques. Optimization of cryoprotectants their concentrations revealed 10 % dimethyl sulfoxide (ME2SO) effective as a cryoprotectant both methods. optimal time equilibration the before was determined be 15 min. Thawing temperature had significant effect on cell recovery viability, being markedly higher when were thawed at °C than 27 °C. After long-term storage liquid nitrogen, preserved by exhibited viability rates those using method, with up 86.4 ± 5.9 6 months. Therefore, ME2SO method recommended protocol cryopreservation M. rosenbergii spermatogonia.

Language: Английский

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Change in energy-consuming strategy, nucleolar metabolism and physical defense in Macrobrachium rosenbergii after acute and chronic polystyrene nanoparticles exposure

Aquatic Toxicology, Journal Year: 2023, Volume and Issue: 263, P. 106711 - 106711

Published: Sept. 29, 2023

Language: Английский

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Dietary protein regulates ovarian development through TOR pathway mediated protein metabolism in female Litopenaeus vannamei

Aquaculture Reports, Journal Year: 2023, Volume and Issue: 33, P. 101781 - 101781

Published: Oct. 16, 2023

The accumulation of nutrients in the gonads is closely related to reproductive capacity and quality larvae subsequent autotrophic stage Pacific white shrimp, Litopenaeus vannamei. Nutrition one important factors affecting gonad development aquaculture animals, dietary protein utilized as major component vitellogenin through vitellogenesis, which acts an indispensable nutrient ovary development. Therefore, six different diets were formulate contain 39.81%, 44.34%, 48.20%, 52.81%, 56.12% 59.45%, respectively. A total 540 shrimp (initial weight, 9.3 g) distributed randomly into eighteen 250-L cylindrical fiber-glass tanks, with each diet assigned 3 replicate tanks. results showed Arg, His, Leu, Lys, Glu, Gly, Pro, essential amino acids (ΣEAA) non-essential (ΣNEAA) significantly influenced by levels, shrimps fed 39.81% exhibited lowest composition among all treatments. percentage ovarian stages III-V was higher ( ≥ 48.20%) than those lower protein. Shrimp 52.81% had serum concentrations (VTG), meanwhile, expression levels 1 (vg1), vtg receptor (vgr) ovaries other diets. However, highest VTG hepatopancreas observed at target rapamycin (torc1), ras homologue enriched brain (rheb), kinase B (akt), 4E-binding (4ebp) phosphatidylinositol 3-kinase (pi3k) up-regulated In conclusion, promoted synthesis exogenous its transport serum, also motivated production endogenous vitellinogen ovary, involved regulation TOR mediated metabolism L.vannamei .

Language: Английский

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Quantitative analysis of infectious precocity virus load and stunted growth syndrome via an immunohistochemical assay and reverse transcriptase quantitative real-time PCR in Macrobrachium rosenbergii

Aquaculture, Journal Year: 2024, Volume and Issue: 583, P. 740577 - 740577

Published: Jan. 11, 2024

Language: Английский

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Efficacy of Giant River Prawn Macrobrachium rosenbergii in Controlling the Invasive Snail Pomacea canaliculata: Implications for Ecological Farming

Diversity, Journal Year: 2023, Volume and Issue: 15(9), P. 1001 - 1001

Published: Sept. 7, 2023

The invasive golden apple snail Pomacea canaliculata has a strong reproductive capacity and rapidly spread in Asian countries. Current control methods include physical, chemical, biological approaches, but there been limited research on the of P. its different life stages. This study assessed effectiveness using giant river prawns Macrobrachium rosenbergii controlling juveniles through controlled indoor experiment. density, size, dispersal range recently hatched juvenile snails were significantly lower among those kept with than without prawns, indicating effect M. at least juveniles. Furthermore, speculates potential application context rice–prawn symbiotic system ecological farming to canaliculata. In terms safety, might lead win-win situation for both rice-farm profits benefits species control.

Language: Английский

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Isolation and characterization of a lectin-like chitinase from the hepatopancreas of freshwater prawn, Macrobrachium rosenbergii

Biochimie, Journal Year: 2023, Volume and Issue: 221, P. 125 - 136

Published: Sept. 27, 2023

Language: Английский

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Transcriptome analysis provides insights into the high ability to synthesize fatty acids in “yellow oil” mud crab (Scylla paramamosain)

Food Research International, Journal Year: 2023, Volume and Issue: 172, P. 113213 - 113213

Published: June 30, 2023

Language: Английский

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