Estimation of median LC50 and toxicity of environmentally relevant concentrations of thiram in Labeo rohita

npj Clean Water, Journal Year: 2025, Volume and Issue: 8(1)

Published: Feb. 8, 2025

Thiram is a widely used fungicide in agricultural practices as spraying on crops and grains storage but its potential adverse effects non-target aquatic organisms have raised serious concerns. In this study, firstly LC50 value of thiram has been determined 0.744 mg/L for the first time Labeo rohita then, fish were exposed to different sub-lethal concentrations (40 μg/L, 80 μg/L 120 μg/L) evaluate tissue growth, oxidative stress, anti-oxidant enzymes histo-pathological parameters at days-20, 40 60 experiment. There observed significant (p < 0.05) decrease body weight while increase relative absolute weights kidneys, heart brain was found. Hematological analysis showed leukocytes neutrophils lymphocytes monocytes, RBCs hemoglobin concentration significantly decreased. Serum biochemical revealed urea hepatic total proteins, albumin creatinine higher doses (80 thiram. Oxidative stress parameters; ROS TBARS increased antioxidant (CAT, SOD, POD, GSH) treated groups compared control. Moreover, comet assay DNA damage isolated cells that further confirmed by alterations these tissues. The findings demonstrate severely pollute marine eco-system quite hazardous species causing severe health even thus, render apparently clean water be unfit animal human consumption.

Language: Английский

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Acute health symptoms related to perception and practice of pesticides use among farmers from all regions of Thailand

Frontiers in Public Health, Journal Year: 2024, Volume and Issue: 11

Published: Jan. 8, 2024

Introduction Occupational exposure to pesticides may cause acute health effects for farmers and agricultural workers. Therefore, this study aims investigate the prevalence of poisoning symptoms related pesticide among from all regions Thailand, as well factors linked neurological neuromuscular systems, respiratory system, eye skin disorders. Methods A cross sectional was conducted in 4,035 who lived four Thailand. The samples were chosen using stratified random sampling, with 746 Central region, 2,065 North-East, 586 North, 638 South. Results results found that highest association systems (75%), followed by system (60.4%), eyes (41.2%), (14.8%). most prevalent muscle pain (49%) symptoms, burning nose (37.6%) itchy (26.3%) rashes (14.4%) symptoms. remarkable findings types use, task on farm, sprayers, perception are crucial affecting Discussion also beneficial Thai government other relevant organizations launching measures, campaigns, or interventions lower modifiable risk factors, resulting reducing risks associated exposure.

Language: Английский

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Acrylamide exposure induces growth retardation, neurotoxicity, stress, and immune/antioxidant disruption in Nile tilapia (Oreochromis niloticus): The alleviative effects of Chlorella vulgaris diets

Fish & Shellfish Immunology, Journal Year: 2024, Volume and Issue: 146, P. 109411 - 109411

Published: Jan. 30, 2024

Language: Английский

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Lead toxicity in African catfish: Promising role of magnetite nanogel against etho-neurological alterations, antioxidant suppression, gene toxicity, and histopathological/ immunohistochemical disruptions

Aquaculture, Journal Year: 2024, Volume and Issue: 594, P. 741411 - 741411

Published: July 30, 2024

Language: Английский

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Hexaflumuron insecticide exposure induces behavior alterations, hemato-biochemical disorders, antioxidant-immune dysfunction, and histopathological alterations in Nile tilapia (Oreochromis niloticus)

Veterinary Research Communications, Journal Year: 2024, Volume and Issue: 48(5), P. 3105 - 3120

Published: July 31, 2024

Language: Английский

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Immunosuppression, growth retardation, and transcriptomic profile alterations induced by chronic copper toxicity in Clarias gariepinus: The ameliorative effect of zinc oxide nanoparticles

Aquaculture Reports, Journal Year: 2024, Volume and Issue: 35, P. 102029 - 102029

Published: March 13, 2024

This study was designated to look into the toxicological effects of long-term exposure African catfish (Clarias gariepinus) a heavy metal (copper) on growth, hematology, immune-antioxidant functions and their regulating genes, resistance Aeromonas sobria. Furthermore, efficiency dietary zinc oxide nanoparticles (ZNPs) ameliorate copper toxicity assessed. Fish (n=300: average body weight: 90.56±0.20 g) were equally allocated six groups in five replicates (10 fish/replicate; 50 fish/ group) for 60 days at water temperature 25 ± 1.2 ◦C. Three diets formulated with varying ZNPs levels: control (0 mg kg−1), ZNPs20 (20 ZNPs30 (30 actual concentrations 45, 65, 75 kg−1, respectively. The experimental named, control, ZNPs20, which fed diets, respectively without exposure. Additionally, CuCL2, CuCL2+ZNPs20, CuCL2+ZNPs30 received same feeding regime as groups, exposed chloride, CuCL2 L−1). At end trial (60 days), all challenged 0.2 mL A. sobria (1×108 CFU mL−1) mortalities recorded daily additional 7 days. results displayed that resulted lower growth metrics survivability. final weight, weight gain, feed intake, survivability reduced group by 39.29%, 23.79%, 63.37%, 20%, increasing conversion ratio 52.13% compared group. In addition, hematological (red blood cells (58.40%), hemoglobin (48.11%), packed cell volume (40.59%), white (46.85%), total proteins (43.12%), albumin (41.98%), globulin (44.23%)), immune (lysozymes (59.74%), complement 3 (41.89%), nitric (57.14%), phagocytic activity (38.75%)), antioxidant parameters (glutathione peroxidase (82.95%), catalase (74.08%), superoxide dismutase (48.59%)), well higher oxidative (malondialdehyde) (200.30%) indicator observed A down-regulation peroxidase-1) (lysozyme-G, lysozyme-C, transforming factor-beta, myeloperoxidase) genes up-regulation inflammatory cytokines (interleukin-1ß, interleukin-8, tumor necrosis factor-alpha, nuclear factor kappa β, Toll-like receptor 5) noticed during challenge (25%) Noticeably, modulated survival, antioxidant/immune associated copper-exposed fish. increased fish challenge. especially 30 kg−1 level proposed promising additive mitigating stress consequences catfish.

Language: Английский

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Ameliorative role of camel protein hydrolysates diet against alkaline stress in Oreochrmis niloticus: Hematology, immune responses and their regulating genes expression, and histopathological assays

Veterinary Research Communications, Journal Year: 2025, Volume and Issue: 49(2)

Published: Jan. 16, 2025

Language: Английский

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Interactive effects of carbendazim and cold shock on haematology and behaviour of juvenile Labeo rohita

Discover Environment, Journal Year: 2025, Volume and Issue: 3(1)

Published: April 19, 2025

Language: Английский

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Therapeutic effects of white poplar ( Populus alba ) leave extract on hepatorenal, stress, and antioxidant-immune parameters of Oreochromis niloticus challenged with Aeromonas veronii

Italian Journal of Animal Science, Journal Year: 2024, Volume and Issue: 23(1), P. 721 - 733

Published: May 14, 2024

Herbal plants have gained enormous interest due to their immune, antioxidant, and antibacterial activities. The present study proposed that white poplar (Populus alba) leave extract (WPE) might provide a promising alternative traditional antibiotics treat Aeromonas veronii infection in Nile tilapia (Oreochromis niloticus). WPE showed an vitro activity [22 ± 0.45 mm inhibition zone, with minimum inhibitory concentration (MIC) bactericidal (MBC) of 60 µg/mL] against A. veronii. For the in-vivo study, factorial (2 × 2) design was used investigate therapeutic effects on antioxidant/immune status blood biochemical parameters challenged One-hundred sixty fish (33 1.5 g) were assigned into four experimental groups, each replicates (4 glass tanks/group, 40 fish/group, 10 fish/tank) for days. In first (1st) 2nd 0 mg/L applied tank water, respectively, without being challenged. 3rd 4th intraperitoneal inoculated 0.2 mL (0.5 107 CFU). significantly diminished survivability, hepatic catalase, lysozyme activity, nitric oxide, immunoglobulin M, phagocytic %, total protein, albumin, globulin. Moreover, significant rise malondialdehyde, serum hepatorenal function indicators, cortisol, glucose levels consequences challenge. Interestingly, interaction between bacterial challenge application increased antioxidant immune responses reduced ALT treated during creatinine, decreased but still higher than non-infected either or not WPE. conclusion, (1.5 mg/L) can be as substance farming alleviate detrimental impacts by enhancing infected fish. These outcomes could help reduce antibiotic usage farms, which is conducive sustainable development aquaculture.

Language: Английский

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Pesticide contamination and associated health risks in fish from Libga and Builpela reservoirs, Northern Ghana

Deleted Journal, Journal Year: 2025, Volume and Issue: 2(1)

Published: March 5, 2025

Abstract Background Pesticide contamination in aquatic ecosystems due to intensive agriculture poses significant health risks communities reliant on fish as a dietary staple. In northern Ghana, where drives high pesticide use, residues from application persist water bodies. Libga and Builpela reservoirs, important for local livelihoods sources are exposed agricultural effluents. Despite their importance, limited data exist these nor have the associated surrounding been quantified. Persistent pesticides of particular concern. This study assessed human Clarias (C.) anguillaris (African catfish) Oreochromis (O.) niloticus (Nile tilapia) reservoirs which receive substantial runoff. Methods Fish samples both were analysed 42 using Liquid Chromatography-Tandem Mass Spectrometry (LC–MS/MS). Lifetime cancer risk, hazard quotient, ratio computed evaluate linked consumption. Two species fish, anguilaris , tested contamination. Results Four detected: pirimiphos-methyl, chlorpyriphos, p,p'-DDE, p,p'-DDD. exhibited higher organophosphate levels with pirimiphos-methyl: showing highest concentration at 0.05 ± 0.02 mg/kg 0.04 0.00 reservoir. showed elevated organochlorines (p,p'-DDD DDD: 0.03 0.01 mg/kg). Organochlorines dominated load (55–60%), p,p'-DDE contributing 27.9% total. The quotient indicated little adverse impacts. However, cumulative LCR values p,p'-DDD potential lifetime chronic exposure. Conclusions our study, though short-term consumption minimal non-carcinogenic risks, consuming C. O. long-term intake may elevate persistent organochlorines. Regular monitoring, public advisories, stricter regulations necessary mitigate protect vulnerable Ghana.

Language: Английский

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