Irradiation-induced hair graying in mice: an experimental model to evaluate the effectiveness of interventions targeting oxidative stress, DNA damage prevention, and cellular senescence

GeroScience, Journal Year: 2024, Volume and Issue: 46(3), P. 3105 - 3122

Published: Jan. 6, 2024

Abstract Hair graying, also known as canities or achromotrichia, is a natural phenomenon associated with aging and influenced by external factors such stress, environmental toxicants, radiation exposure. Understanding the mechanisms underlying hair graying an ideal approach for developing interventions to prevent reverse age-related changes in regenerative tissues. induced ionizing (γ-rays X-rays) has emerged valuable experimental model investigate molecular pathways involved this process. In review, we examine existing evidence on radiation-induced particular focus potential role of cellular senescence. We explore current understanding aging, delve into mechanisms, highlight unique advantages using ionizing-irradiation–induced research model. By elucidating involved, aim deepen our potentially identify novel therapeutic targets address phenotypic change.

Language: Английский

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Mushroom Tyrosinase: Six Isoenzymes Catalyzing Distinct Reactions

ChemBioChem, Journal Year: 2024, Volume and Issue: 25(14)

Published: Feb. 22, 2024

"Mushroom tyrosinase" from the common button mushroom is most frequently used source of tyrosinase activity, both for basic and applied research. Here, complete family Agaricus bisporus var. (abPPO1-6) was cloned mRNA expressed heterologously using a single protocol. All six isoenzymes accept wide range phenolic catecholic substrates, but display pronounced differences in their specificity enzymatic reaction rate. AbPPO3 ignores γ-l-glutaminyl-4-hydroxybenzene (GHB), natural phenol present mM concentrations A. bisporus, while AbPPO4 processes 100 μM GHB at 4-times rate catechol l-DOPA. AbPPOs are biochemically distinct enzymes fit different roles fungal life cycle, which challenges traditional concept as catalyzing same physiological varying only secondary properties. Transferring this approach to other organisms will greatly stimulate study vivo function(s) application these highly efficient catalysts.

Language: Английский

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Comparative Analysis of Olive-Derived Phenolic Compounds’ Pro-Melanogenesis Effects on B16F10 Cells and Epidermal Human Melanocytes

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(8), P. 4479 - 4479

Published: April 19, 2024

Olive leaf contains plenty of phenolic compounds, among which oleuropein (OP) is the main component and belongs to group secoiridoids. Additionally, compounds such as oleocanthal (OL) oleacein (OC), share a structural similarity with OP two aldehyde groups, are also present in olive leaves. These have been studied for several health benefits, anti-cancer antioxidant effects. However, their impact on skin remains unknown. Therefore, this study aims compare effects these three melanogenesis using B16F10 cells human epidermal cells. Thousands gene expressions were measured by global expression profiling We found that glutaraldehyde derived from leaves potential effect activation pathway inducing differentiation Accordingly, pro-melanogenesis was investigated means melanin quantification, mRNA, protein melanocytes (HEM). This suggests secoiridoid its derivates an protection promoting production both mouse cell lines.

Language: Английский

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Unveiling the Potential of Ultrasonic-Assisted Ethanol Extract from Sargassum horneri in Inhibiting Tyrosinase Activity and Melanin Production in B16F10 Murine Melanocytes

Frontiers in Bioscience-Landmark, Journal Year: 2024, Volume and Issue: 29(5)

Published: May 20, 2024

Backgrounds: Melanogenesis, regulated by genetic, hormonal, and environmental factors, occurs in melanocytes the basal layer of epidermis. Dysregulation this process can lead to various skin disorders, such as hyperpigmentation hypopigmentation. Therefore, present study investigated effect ultrasonic-assisted ethanol extract (SHUE) from Sargassum horneri (S. horneri), brown seaweed against melanogenesis α-melanocyte-stimulating hormone (MSH)-stimulated B16F10 murine melanocytes. Methods: Firstly, yield proximate compositional analysis samples were conducted. The SHUE on cell viability has been evaluated using 3-(4,5-Dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide (MTT) assay. After that, melanin content cellular tyrosinase activity α-MSH-stimulated examined. Western blot was carried out investigate protein expression levels microphthalmia-associated transcription factor (MITF), tyrosinase, tyrosinase-related protein-1 (TRP1), protein-2 (TRP2). In addition, extracellular signal-regulated kinase (ERK) assessed via blotting. Results: As per analysis, contained highest average a dry basis at 28.70 ± 3.21%. findings showed that reduced Additionally, MITF, TRP1, TRP2 significantly downregulated treatment Moreover, upregulated phosphorylation ERK AKT experiments conducted inhibitor (PD98059) revealed depends signaling cascade. Conclusion: These results suggest an anti-melanogenic be used material formulation cosmetics related whitening lightening.

Language: Английский

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Epigenetic modifications in spinal ligament aging

Ageing Research Reviews, Journal Year: 2022, Volume and Issue: 77, P. 101598 - 101598

Published: Feb. 24, 2022

Spinal stenosis is a common degenerative spine disorder in the aged population and spinal ligament aging main contributor to this chronic disease. However, underlying mechanisms of remain unclear. Epigenetics study heritable reversible changes function gene or genome that occur without any alteration primary DNA sequence. Epigenetic alterations have been demonstrated play crucial roles age-related diseases conditions, they are recently studied as biomarkers therapeutic targets field cancer research. The epigenetic modifications, including methylation alteration, histone modifications well dysregulated noncoding RNA modulation, all implicated diseases. modulates expression critical genes WNT5A, GDNF, ACSM5, miR-497 miR-195 during degeneration. Histone widely affect obvious modification abnormalities found aging. MicroRNAs (miRNAs), long RNAs (lncRNAs) circular (circRNAs) exert regulating effects on conditions via targeting various osteogenic fibrogenic differentiation related genes. To our knowledge, there no systematic review yet summarize involvement In study, we systematically discussed different their potential functions process.

Language: Английский

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Zebrafish in dermatology: a comprehensive review of their role in investigating abnormal skin pigmentation mechanisms

Frontiers in Physiology, Journal Year: 2023, Volume and Issue: 14

Published: Nov. 23, 2023

Skin pigmentation abnormalities, ranging from aesthetic concerns to severe hyperpigmentation disease, have profound implications for individuals’ psychological and economic wellbeing. The intricate etiology of our evolving comprehension its underlying mechanisms underscore the need robust animal models. Zebrafish, renowned their transparent embryos genetic parallels humans, been spotlighted as a pivotal model skin studies. This review offers concise overview zebrafish attributes, highlighting shared melanin production pathways with humans. We systematically dissect diverse strategies craft models abnormal pigmentation, spanning physical, chemical, interventions, while critically appraising merits constraints each approach. Additionally, we elucidate metrics employed gauge efficacy these Concluding, cast visionary gaze on prospective breakthroughs in domain, aiming steer forthcoming efforts refined research.

Language: Английский

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Interruption of p38^MAPK-MSK1-CREB-MITF-M pathway to prevent hyperpigmentation in the skin

International Journal of Biological Sciences, Journal Year: 2024, Volume and Issue: 20(5), P. 1688 - 1704

Published: Jan. 1, 2024

Background: Melanocortin 1 receptor (MC1R), a of α-melanocyte-stimulating hormone (α-MSH), is exclusively present in melanocytes where α-MSH/MC1R stimulate melanin pigmentation through microphthalmia-associated transcription factor M (MITF-M).Toll-like 4 (TLR4), endotoxin lipopolysaccharide (LPS), distributed immune and other cell types including LPS/TLR4 activate transcriptional activity nuclear (NF)-κB to express cytokines innate immunity.LPS/TLR4 also up-regulate MITF-M-target melanogenic genes melanocytes.Here, we propose molecular target antimelanogenic elucidating inhibitory mechanism on α-MSH-induced programs by benzimidazole-2-butanol (BI2B), an inhibitor LPS/TLR4-activated NF-κB.Methods: Ultraviolet B (UV-B)-irradiated skins HRM-2 hairless mice α-MSH-activated melanocyte cultures were employed examine programs.Results: Topical treatment with BI2B ameliorated UV-B-irradiated skin hyperpigmentation mice.BI2B suppressed the protein or mRNA levels markers, such as tyrosinase (TYR), MITF-M proopiomelanocortin (POMC), UV-B-exposed pigmented tissues.Moreover, inhibited co-cultures keratinocyte cells that cultures.Mechanistically, activation cAMP response element-binding (CREB) expression at promoter level.As target, primarily mitogen-activated kinase (MAPK) 3 (MKK3)-catalyzed p38 MAPK .Subsequently, interrupted downstream pathway -mitogen stress-activated kinase-1 (MSK1)-CREB-MITF-M, genes, encoding enzymes TYR, TYR-related protein-1 (TRP-1) dopachrome tautomerase (DCT) biosynthesis, proteins PMEL17 Rab27A transfer melanosomes overlaying keratinocytes skin.Conclusion: Targeting MKK3-p38 -MSK1-CREB-MITF-M was suggested rationale inhibit UV-B-or facultative melanogenesis strategy prevent acquired pigmentary disorders skin.

Language: Английский

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Investigating the impact of pigmentation variation of breast muscle on growth traits, melanin deposition, and gene expression in Xuefeng black-bone chickens

Poultry Science, Journal Year: 2024, Volume and Issue: 103(6), P. 103691 - 103691

Published: March 24, 2024

The blackness traits, considered an important economic factor in the black-bone chicken industry, still exhibits a common phenomenon of significant difference breast muscle. To improve this phenomenon, study compared growth and transcriptome muscles between High Blackness Group (H group) Low (L Xuefeng chickens. results are as follows: 1) There was no traits H group L (P > 0.05). 2) skin/breast muscle values were significantly lower than those group, while melanin content exhibited opposite trend < 3) A negative correlation observed value 0.05), skin exhibiting positive with 4) comparison revealed 831 405 DEGs female male chickens, respectively. This included 37 shared enriched melanosome, pigment granule, melanogenesis pathway. Seven candidate genes (DCT, PMEL, MLANA, TYRP1, OCA2, EDNRB2, CALML4) may play crucial role production chicken. findings could accelerate breeding process for achieving desired levels contribute to exploration mechanisms underlying

Language: Английский

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Immune pathogenic response landscape of acute posterior multifocal placoid pigment epitheliopathy revealed by scRNA sequencing

Genes and Immunity, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 7, 2025

Language: Английский

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Acetylation-enhanced Sp1 transcriptional activity suppresses Mlph expression

Scientific Reports, Journal Year: 2025, Volume and Issue: 15(1)

Published: Jan. 17, 2025

Melanosome transport is regulated by major proteins, including Rab27a, Melanophilin (Mlph), and Myosin Va (Myo-Va), that form a tripartite complex. Mutation of these proteins causes melanosome aggregation around the nucleus. Among Mlph linker between Rab27a Myo-Va. There are some studies about regulation transcriptional expression. However, its post-translational modifications remains unclear. In this study, inhibition HDACs SAHA TSA disrupted transport, causing aggregation. Specifically, we identified novel mechanism in which HDAC5 regulates expression via Sp1. Knockdown increased acetylation Sp1 binding to promoter, thereby modulating This study highlights crucial role through interaction with These findings suggest HDAC5-mediated deacetylation pivotal modification providing insights into molecular mechanisms underlying process.

Language: Английский

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