Self‐Mineralizing Dnazyme Hydrogel as a Multifaceted Bone Microenvironment Amendment for Promoting Osteogenesis in Osteoporosis

Advanced Functional Materials, Journal Year: 2023, Volume and Issue: 33(19)

Published: Feb. 26, 2023

Abstract The accumulation of reactive oxygen species (ROS) and minimal osteogenic raw material in the osteoporotic bone microenvironment greatly inhibits activity osteoblasts. Herein, it is originally proposed to construct a biomatrix multifaceted amendment ‐Mineralized zippered G4‐Hemin DNAzyme hydrogel (MDH)‐to improve capacity promote high‐quality defect repair. programmed design rolling circle amplified DNA synthesis system allows introduction massive amounts MDH. highly mimics tight catalytic configuration horseradish peroxidase exerts excellent enzyme‐like with considerable ROS molecule scavenging ability. In addition, amplification by‐product pyrophosphate ingeniously employed as sufficient phosphorus source, thus constituting an autonomous mineralization for waste reuse through hydrolase calcium ions, which deposits MDH addresses challenge bio‐application stability. remarkable vitro vivo outcomes demonstrate that can effectively oxidative stress status osteoblasts, restore balance mitochondrial membrane potential, reduce apoptosis, ultimately demonstrating superior capacity.

Language: Английский

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Ubiquitin‐specific peptidases: Players in bone metabolism

Cell Proliferation, Journal Year: 2023, Volume and Issue: 56(8)

Published: March 8, 2023

Abstract Osteoporosis is an ageing‐related disease, that has become a major public health problem and its pathogenesis not yet been fully elucidated. Substantial evidence suggests strong link between overall age‐related disease progression epigenetic modifications throughout the life cycle. As important modification, ubiquitination extensively involved in various physiological processes, role bone metabolism attracted increasing attention. Ubiquitination can be reversed by deubiquitinases, which counteract protein degradation. largest most structurally diverse cysteinase family of deubiquitinating enzymes, ubiquitin‐specific proteases (USPs), comprising cysteine kinase have found to players maintaining balance formation resorption. The aim this review explore recent findings highlighting regulatory functions USPs provide insight into molecular mechanisms governing their actions during loss. An in‐deep understanding USPs‐mediated regulation resorption will scientific rationale for discovery development novel USP‐targeted therapeutic strategies osteoporosis.

Language: Английский

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Age-related decline in melatonin contributes to enhanced osteoclastogenesis via disruption of redox homeostasis

Molecular Medicine, Journal Year: 2024, Volume and Issue: 30(1)

Published: Jan. 12, 2024

Abstract Background Increased oxidative stress contributes to enhanced osteoclastogenesis and age-related bone loss. Melatonin (MT) is an endogenous antioxidant declines with aging. However, it was unclear whether the decline of MT involved in during aging process. Methods The plasma level MT, status, mass, number marrow-derived monocytes (BMMs) its were analyzed young (3-month old) old (18-month mice (n = 6 per group). In vitro, BMMs isolated from aged treated or without followed by detecting change intracellular reactive oxygen species (ROS) level. Furthermore, for 2 months investigate therapeutic effect. Results markedly lower compared mice. Age-related accompanied stress, osteoclastogenic potential intervention significantly suppressed receptor activator nuclear factor-κB ligand (RANKL)-induced osteoclastogenesis, decreased ROS capacity supplementation attenuated loss deterioration microstructure Conclusions These results suggest that via disruption redox homeostasis. may serve as a key regulator homeostasis, thereby highlighting preventive agent

Language: Английский

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Latest Progress of LIPUS in Fracture Healing

Journal of Ultrasound in Medicine, Journal Year: 2024, Volume and Issue: 43(4), P. 643 - 655

Published: Jan. 15, 2024

The use of low‐intensity pulsed ultrasound (LIPUS) for promoting fracture healing has been Food and Drug Administration (FDA)‐approved since 1994 due to largely its non‐thermal effects sound flow radiation force so on. Numerous clinical animal studies have shown that LIPUS can accelerate the fresh fractures, nonunions, delayed unions in pulse mode regardless devices or circumstantial factors. Rare show limitations treating fractures with intramedullary nail fixation low patient compliance. biological effect is achieved by regulating various cellular behaviors involving mesenchymal stem/stromal cells (MSCs), osteoblasts, chondrocytes, osteoclasts dose dependency on intensity time. Specifically, promotes osteogenic differentiation MSCs through ROCK‐Cot/Tpl2‐MEK–ERK signaling. Osteoblasts, turn, respond mechanical signal integrin, angiotensin type 1 (AT1), PIEZO1 mechano‐receptors, leading production inflammatory factors such as COX‐2, MCP‐1, MIP‐1β repair. also induces CCN2 expression chondrocytes thereby coordinating bone regeneration. Finally, suppresses osteoclast gene interfering ERK/c‐Fos/NFATc1 cascade. This mini‐review revisits known mechanisms strengthens need further investigation into underlying mechanisms.

Language: Английский

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Exogenous iron caused osteocyte apoptosis, increased RANKL production, and stimulated bone resorption through oxidative stress in a murine model

Chemico-Biological Interactions, Journal Year: 2024, Volume and Issue: 399, P. 111135 - 111135

Published: July 4, 2024

Language: Английский

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