New Insights into the Development of Donepezil-Based Hybrid and Natural Molecules as Multi-Target Drug Agents for Alzheimer’s Disease Treatment DOI Creative Commons
Violina T. Angelova, Boris Petrov Stoyanov, Rumyana Simeonova

et al.

Molecules, Journal Year: 2024, Volume and Issue: 29(22), P. 5314 - 5314

Published: Nov. 11, 2024

Alzheimer's disease (AD) involves a complex pathophysiology with multiple interconnected subpathologies, including protein aggregation, impaired neurotransmission, oxidative stress, and microglia-mediated neuroinflammation. Current treatments, which generally target single subpathology, have failed to modify the disease's progression, providing only temporary symptom relief. Multi-target drugs (MTDs) address several aggregation of pathological proteins. In this review, we cover hybrid molecules published between 2014 2024. We offer an overview strategies employed in drug design approaches that led notable improvements reduced hepatotoxicity. Our aim is insights into potential development new drugs. This highlights multi-target featuring heterocycles

Language: Английский

Cannabidiol (CBD) Acts as an Antioxidant on Gardnerella vaginalis, Resulting in Reduced Metabolic Activity, Loss of Survivability, and Elimination of Biofilms DOI Creative Commons
Ronit Vogt Sionov, Maya Korem, Itzhack Polacheck

et al.

Antibiotics, Journal Year: 2025, Volume and Issue: 14(2), P. 136 - 136

Published: Feb. 1, 2025

Background: Gardnerella vaginalis is a natural inhabitant of the vagina, but when an imbalance occurs in vaginal microbiota, this bacterium can cause vaginosis, condition that must be treated symptomatic and prior to gynecological intervention. Cannabidiol (CBD) anti-inflammatory compound also has antibacterial activities against several Gram-positive certain Gram-negative bacteria. Objectives: Since G. opportunistic pathogenic Gram-variable bacterium, we investigated its response CBD. Methods: The activity CBD was studied by broth dilution assay, changes intracellular ATP levels, ability bacteria recover on chocolate agar plates. antibiofilm MTT metabolic crystal violet staining, HR-SEM. Flow cytometric analyses were performed measure membrane potential, perforation, activity. Reactive oxygen species (ROS) production analyzed using nitro blue tetrazolium (NBT) reagent. Gene expression determined semi-quantitative real-time PCR, while protein composition LC-MS/MS analysis. Results: We observed clinical isolates exhibited high susceptibility with minimum inhibitory concentration (MIC) 2.5 µg/mL induced rapid hyperpolarization caused cytoplasmic leakage without increasing propidium iodide uptake. This accompanied reduced loss survivability. Proteomic analysis revealed decreased some ribosomal-associated proteins. antioxidant reducing ROS levels dose-dependent manner. effect neutralized free radical scavenger α-tocopherol, suggesting involvement radicals executing effect. Importantly, not only prevented biofilm formation biomass preformed, mature biofilms. Real-time PCR for 6 h showed increase biofilm-associated genes, mainly due did alter adhere HeLa cervical carcinoma cells CBD-treated still phagocytosed RAW264.7 macrophages. Conclusions: Our study shows exhibits thus potential drug treatment vaginosis bacterium.

Language: Английский

Citations

1
Surface-enhanced Raman scattering for the detection of biomarkers of neurodegenerative diseases: A review DOI
Chentao Li,

Yinglin Wang,

Yafang Wu

et al.

TrAC Trends in Analytical Chemistry, Journal Year: 2025, Volume and Issue: 185, P. 118173 - 118173

Published: Feb. 3, 2025

Language: Английский

Citations

0
A predictive machine learning model for cannabinoid effect based on image detection of reactive oxygen species in microglia DOI Creative Commons
Patricia Sinclair,

William Jeffries,

Nadege Lebert

et al.

PLoS ONE, Journal Year: 2025, Volume and Issue: 20(3), P. e0320219 - e0320219

Published: March 25, 2025

Neuroinflammation is a key feature of human neurodisease including neuropathy and neurodegenerative disease driven by the activation microglia, immune cells nervous system. During microglia release pro-inflammatory cytokines as well reactive oxygen species (ROS) that can drive local neuronal glial damage. Phytocannabinoids are an important class naturally occurring compounds found in cannabis plant ( Cannabis sativa ) interact with body’s endocannabinoid receptor Cannabidiol (CBD) prototype phytocannabinoid anti-inflammatory properties observed animal models. We measured ROS (HMC3) using CellROX, fluorescent dynamic indicator. tested effect CBD on level presence three known activators: lipopolysaccharide (LPS), amyloid beta (A β 42 ), immunodeficiency virus (HIV) glycoprotein (GP120). Confocal microscopy images within were coupled to deep learning model convolutional neural network (CNN) predict responses. Our study demonstrates platform be used assessment image measure.

Language: Английский

Citations

0
Cannabis for medicine and food: A benefit vs risk critical appraisal DOI Creative Commons
Murendeni Nethengwe,

Y Maphosa,

BO Ahiante

et al.

South African Journal of Botany, Journal Year: 2024, Volume and Issue: 175, P. 15 - 31

Published: Oct. 11, 2024

Language: Английский

Citations

1
Bioactive Three-Dimensional Chitosan-Based Scaffolds Modified with Poly(dopamine)/CBD@Pt/Au/PVP Nanoparticles as Potential NGCs Applicable in Nervous Tissue Regeneration—Preparation and Characterization DOI Creative Commons
Aleksandra Sierakowska-Byczek, Adam Gałuszka,

Łukasz Janus

et al.

Molecules, Journal Year: 2024, Volume and Issue: 29(22), P. 5376 - 5376

Published: Nov. 14, 2024

Tissue engineering of nervous tissue is a promising direction in the treatment neurological diseases such as spinal cord injuries or neuropathies. Thanks to technological progress and scientific achievements; use cells; artificial scaffolds; growth factors are becoming increasingly common. Despite challenges complex structure this tissue, regenerative medicine appears future approach improve quality life patients with injuries. Until now; most functional biomaterials used for purpose were based on decellularized extra cellular matrix (ECM) nanofibrous materials, whereas current clinically verified ones cases do not exhibit bioactivity possibility external stimulation. The aim research was develop new type bioactive, chitosan-based 3D materials applicable nerve guide conduits (NGCs) modified poly(dopamine), Au/Pt coated PVP nanoparticles, cannabidiol. NGCs prepared under microwave-assisted conditions their chemical studied using FT-IR method. Next, study will discuss novel morphology swelling abilities well susceptibility biodegradation presence collagenase lysozyme. Finally, potential field has been via cytotoxicity 1321N1 human astrocytoma cell line, which confirmed biocompatibility direct contact studies.

Language: Английский

Citations

1
Advanced electrochemical detection methodologies for assessing neuroactive substance variability induced by environmental pollutants exposure DOI Creative Commons

Yuanyuan Cao,

Chengkai Yang, Chengshuai Liu

et al.

Environmental Technology & Innovation, Journal Year: 2024, Volume and Issue: unknown, P. 103965 - 103965

Published: Dec. 1, 2024

Language: Английский

Citations

1
O uso terapêutico da Cannabis e seus derivados no tratamento de doenças neurodegenerativas DOI Open Access

Lucas Soares Bezerra de Almeida,

Carolina Parentoni de Oliveira Brêttas,

Thaís Nacur Pimenta

et al.

Brazilian Journal of Health Review, Journal Year: 2024, Volume and Issue: 7(4), P. e71708 - e71708

Published: Aug. 6, 2024

Introdução: Dentre as principais DNs pode-se citar a Doença de Alzheimer, Parkinson, Coreia Huntington, Esclerose Múltipla e Lateral Amiotrófica, todas elas progressivas relacionadas à idade fatores risco como nutrição, infecção, stress oxidativo associadas perda e/ou disfunção neurônios em diferentes regiões do encéfalo. Assim, com milhões pessoas sendo afetadas declínio cognitivo, motora outros sinais sintomas neurológicos, o manejo das doenças esboça um impacto direto no bem-estar dos pacientes seus familiares, constando grande fardo social, salutar econômico, uma vez que exige ampla necessidade cuidados paliativos. À vista disso, diversos estudos foram conduzidos nas últimas décadas acerca uso canabinóides sua relação fitoterapia. Objetivo: Este estudo tem objetivo principal realizar análise atuais sobre Cannabis derivados na terapêutica amplo leque neurodegenerativas, fim verificar real eficácia nível segurança. Métodos: Foi realizada busca bases dados MEDLINE, LILACS SciELO. Na fase identificação, utilizando-se os descritores, encontrados 76 artigos, não duplicados. Sendo assim, tendo base critérios inclusão, filtrados artigos publicados entre Janeiro 2014 Julho 2024, totalizando 66 artigos. Ainda elegibilidade, verificando tema focal da pesquisa, foi excluído 1 artigo estava incompleto. Ao final, selecionados 65 científicos revisões literatura relatos pesquisa. Discussão:Apesar promessas terapêuticas derivados, pesquisa aplicação neurodegenerativas ainda enfrenta várias limitações precisam ser abordadas para consolidar seu clínico. Uma preocupações é variabilidade dosagem formulação produtos utilizados nos estudos, dificulta comparação direta resultados. Além legislação medicinal varia significativamente países, impactando disponibilidade aceitação desses tratamentos. Ademais, maioria ensaios clínicos realizados até agora apresenta tamanhos amostra pequenos períodos acompanhamento relativamente curtos, limita capacidade generalizar achados avaliar efeitos longo prazo. A heterogeneidade desenhos populações também contribui inconsistência É essencial tratamentos sejam acompanhados perto por profissionais saúde garantir benefícios superem riscos, crucial continuar investigação entender completamente mecanismos ação, riscos associados, garantindo baseados evidências robustas seguras. Conclusão: Nota-se mais tema, realização bem projetados grandes amostras prazo, validar preliminares fornecer segurança neurodegenerativas.

Citations

0
New Insights into the Development of Donepezil-Based Hybrid and Natural Molecules as Multi-Target Drug Agents for Alzheimer’s Disease Treatment DOI Creative Commons
Violina T. Angelova, Boris Petrov Stoyanov, Rumyana Simeonova

et al.

Molecules, Journal Year: 2024, Volume and Issue: 29(22), P. 5314 - 5314

Published: Nov. 11, 2024

Alzheimer's disease (AD) involves a complex pathophysiology with multiple interconnected subpathologies, including protein aggregation, impaired neurotransmission, oxidative stress, and microglia-mediated neuroinflammation. Current treatments, which generally target single subpathology, have failed to modify the disease's progression, providing only temporary symptom relief. Multi-target drugs (MTDs) address several aggregation of pathological proteins. In this review, we cover hybrid molecules published between 2014 2024. We offer an overview strategies employed in drug design approaches that led notable improvements reduced hepatotoxicity. Our aim is insights into potential development new drugs. This highlights multi-target featuring heterocycles

Language: Английский

Citations

0