ML365 ameliorates postoperative cognitive impairment in aged mice by inhibiting NLRP3 inflammasome activation in the hippocampus DOI Creative Commons

Zhu Wang,

Bowen Li,

Jingrui Yang

et al.

Brain Research, Journal Year: 2024, Volume and Issue: 1837, P. 148957 - 148957

Published: April 24, 2024

The aim of this study was to examine the effects ML365, a two-pore potassium channel (K2P) inhibitor, on postoperative cognitive impairment (POCD). A mouse model POCD constructed by subjecting aged C57BL/6 mice exploratory laparotomy. Changes in function were assessed using Morris water maze test. Western blotting and qPCR used detect hippocampal NLRP3, Caspase-1 IL-1β expression levels days 3 7 post-surgery. Apoptosis-associated speck-like protein containing caspase recruitment domain (ASC) level also western blotting. Pathological changes nerve damage CA1 CA3 regions detected H&E staining, while concentration malondialdehyde (MDA) plasma measured. We found that pretreatment with ML365 (administered intraperitoneally at dose 10 mg/kg) 30 min prior laparotomy effectively ameliorated mice. reduced Caspase-1, ASC hippocampus, improved POCD-induced pathological areas mice, decreased MDA oxidative stress. Together, our findings indicate can alleviate inhibiting NLRP3 inflammasome activation hippocampus.

Language: Английский

Oligomer sensitive in-situ detection and characterization of gold colloid aggregate formations observed within the hippocampus of the Alzheimer’s disease rat DOI
Kazushige Yokoyama,

Joel Mukkatt,

Nicole Mathewson

et al.

Neuroscience Letters, Journal Year: 2025, Volume and Issue: unknown, P. 138218 - 138218

Published: March 1, 2025

Language: Английский

Citations

0
Repeated sevoflurane exposure causes hypomyelination in the prefrontal cortex of adult male mice DOI Creative Commons

Linyong Zhang,

Z Ke,

Ning Zhang

et al.

Scientific Reports, Journal Year: 2025, Volume and Issue: 15(1)

Published: Jan. 9, 2025

As one of the most commonly used general anesthetics (GAs) in surgery, numerous studies have demonstrated detrimental effects sevoflurane exposure on myelination developing and elderly brain. However, impact intact myelin structure adult brain is barely discovered. Here, we show that repeated exposure, but not single causes hypomyelination abnormal ultrastructure sheath prefrontal cortex (PFC) male mice, which considered as a critical region for anesthesia mediated consciousness change. Furthermore, disrupted proliferation oligodendrocyte precursor cells (OPCs) contributes to exposure-induced defect. This may be owing an accumulated tuberous sclerosis complex 1 (TSC1) expression inhibition mammalian target rapamycin (mTOR) signaling, leading unbalance TSC1-mTORC1 activity after proper central nervous system (CNS). Moreover, aggregates defect cuprizone-induced demyelination model. Together, our present work establishes role integrity PFC mice provides new insight elucidate mechanism GAs-induced dysfunctions.

Language: Английский

Citations

0
Histone Deacetylation in Alzheimer’s Diseases (AD); Hope or Hype DOI

Nabaa Hisham Ateya,

Sarah F. Al-Taie,

Saade Abdalkareem Jasim

et al.

Cell Biochemistry and Biophysics, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 18, 2025

Language: Английский

Citations

0
Global research frontiers and thematic trends in opioid-free anesthesia over the past 20 years: a bibliometric analysis DOI Creative Commons
He Jin, Rong Huang,

Y. Liu

et al.

Frontiers in Pharmacology, Journal Year: 2025, Volume and Issue: 16

Published: April 2, 2025

Opioids have constituted an essential element of general anesthesia for a considerable length time. However, with the increase in opioid misuse and associated postoperative adverse effects, studies related to opioid-free (OFA) emerged, which pose challenge identifying key research directions. Accordingly, objective this study was provide review relevant literature field OFA over past 2 decades, goal prevailing trends Frontiers. A systematic publications on conducted using Web Science Core Collection database, between years 2005 2024. The bibliometric analysis CiteSpace (version 6.1. R6), VOSviewer 1.6.19), R (version4.4.2). In conclusion, 477 were included study. number annual has exhibited steady decades. United States its institutions found be most central. Forget, Patrice, BELOEIL H identified as prolific highly cited authors, respectively. journal highest BMC Anesthesiology. frequently Anesthesia Analgesia, followed by addition, keyword burst, keywords co-occurrence, references indicate that recent focused on: consumption, pain, nausea vomiting (PONV). Meanwhile, clusters timeline view showed main frontiers are sevoflurane anesthesia, plane block, multimodal opioid-sparing anesthesia. Our results show current directions focus PONV. Frontiers future expected include areas

Language: Английский

Citations

0
Updated insights into the NLRP3 inflammasome in postoperative cognitive dysfunction: emerging mechanisms and treatments DOI Creative Commons
Tian Wang,

Guangwei Sun,

Bingdong Tao

et al.

Frontiers in Aging Neuroscience, Journal Year: 2024, Volume and Issue: 16

Published: Sept. 30, 2024

Postoperative cognitive dysfunction (POCD) poses a significant threat to patients undergoing anesthesia and surgery, particularly elderly patients. It is characterized by diminished functions post such as impaired memory decreased concentration. The potential risk factors for POCD include age, surgical trauma, anesthetic type, overall health condition; however, the precise mechanisms underlying remain elusive. Recent studies suggest that neuroinflammation might be primary pathogenic factor. NOD-, LRR- pyrin domain-containing protein 3 (NLRP3) inflammasomes are implicated in exacerbating promoting release of inflammatory proteins initiate pyroptosis, further influencing disease process. regulation NLRP3 inflammasome activity, including its activation degradation, tightly controlled through multiple pathways mechanisms. In addition, autophagy, protective mechanism, regulates control progression POCD. This review reviews recent findings on role pathogenesis discusses therapeutic strategies aimed at reducing sources, inhibiting cellular enhancing autophagy.

Language: Английский

Citations

2
Chaperones vs. oxidative stress in the pathobiology of ischemic stroke DOI Creative Commons
Vladislav O. Soldatov, А. А. Венедиктов, А. Е. Белых

et al.

Frontiers in Molecular Neuroscience, Journal Year: 2024, Volume and Issue: 17

Published: Dec. 11, 2024

As many proteins prioritize functionality over constancy of structure, a proteome is the shortest stave in Liebig's barrel cell sustainability. In this regard, both prokaryotes and eukaryotes possess abundant machinery supporting quality healthy stressful conditions. This machinery, namely chaperones, assists folding, refolding, utilization client proteins. The functions chaperones are especially important for brain cells, which highly sophisticated terms structural functional organization. Molecular known to exert beneficial effects diseases including one most threatening widespread pathologies, ischemic stroke. However, whether how they antioxidant defense stroke remains unclear. Herein, we discuss shown fight oxidative stress mechanisms their action. stroke, during intense production free radicals, molecular preserve by interacting with oxidized proteins, regulating imbalanced mitochondrial function, directly fighting stress. For instance, cells recruit Hsp60 Hsp70 provide proper folding newly synthesized proteins-these factors required early response refold damaged polypeptides. Additionally, upregulates some dedicated pathways such as FOXO3 signaling. Small HSPs decrease via attenuation function through involvement regulation Nrf- (Hsp22), Akt Hippo (Hsp27) signaling well mitophagy (Hsp27, Hsp22). A similar has also been proposed Sigma-1 receptor, contributing function. Some can prevent excessive formation reactive oxygen species whereas Hsp90 suggested be responsible pro-oxidant Finally, heat-resistant obscure (Hero) able shield thus preventing possible oxidation.

Language: Английский

Citations

2
Dexmedetomidine-mediated improvement of perioperative neurocognitive disorders by miR-184-3p-mediated NLRP3 DOI
Fumou Deng, Bin Zhou, Shenglan Zhang

et al.

Brain Research, Journal Year: 2024, Volume and Issue: 1842, P. 149051 - 149051

Published: June 1, 2024

Language: Английский

Citations

1
tsRNA-5006c regulates hippocampal neurons ferroptosis to ameliorate perioperative neurocognitive disorders in aged male mice DOI
Chengji J. Zhou, Fang Lian,

Hejian Li

et al.

3 Biotech, Journal Year: 2024, Volume and Issue: 15(1)

Published: Dec. 18, 2024

Language: Английский

Citations

1
The Probe Trials in the Water Maze Test Exceeding Two Times May Lead to Extinction DOI

Xiaodong Han,

Xiaobin Liu,

Haiyan Shi

et al.

Published: Jan. 1, 2024

Language: Английский

Citations

0
ML365 ameliorates postoperative cognitive impairment in aged mice by inhibiting NLRP3 inflammasome activation in the hippocampus DOI Creative Commons

Zhu Wang,

Bowen Li,

Jingrui Yang

et al.

Brain Research, Journal Year: 2024, Volume and Issue: 1837, P. 148957 - 148957

Published: April 24, 2024

The aim of this study was to examine the effects ML365, a two-pore potassium channel (K2P) inhibitor, on postoperative cognitive impairment (POCD). A mouse model POCD constructed by subjecting aged C57BL/6 mice exploratory laparotomy. Changes in function were assessed using Morris water maze test. Western blotting and qPCR used detect hippocampal NLRP3, Caspase-1 IL-1β expression levels days 3 7 post-surgery. Apoptosis-associated speck-like protein containing caspase recruitment domain (ASC) level also western blotting. Pathological changes nerve damage CA1 CA3 regions detected H&E staining, while concentration malondialdehyde (MDA) plasma measured. We found that pretreatment with ML365 (administered intraperitoneally at dose 10 mg/kg) 30 min prior laparotomy effectively ameliorated mice. reduced Caspase-1, ASC hippocampus, improved POCD-induced pathological areas mice, decreased MDA oxidative stress. Together, our findings indicate can alleviate inhibiting NLRP3 inflammasome activation hippocampus.

Language: Английский

Citations

0