Theriogenology, Journal Year: 2021, Volume and Issue: 178, P. 8 - 17
Published: Oct. 25, 2021
Language: Английский
International Journal of Molecular Sciences, Journal Year: 2020, Volume and Issue: 21(20), P. 7568 - 7568
Published: Oct. 14, 2020
The participation of extracellular vesicles in many cellular processes, including reproduction, is unquestionable. Although currently, the tetraspanin proteins found are mostly applied as markers, increasing evidence points to their role vesicle biogenesis, cargo selection, cell targeting, and uptake under both physiological pathological conditions. In this review, we bring other insight into involvement physiology mammalian reproduction. We provide knowledge regarding tetraspanins these processes somatic cells. Furthermore, discuss future direction towards an understanding functions tissues fluids reproductive system gamete maturation, fertilization, embryo development; mutual contact communication complexity.
Language: Английский
Citations
165
Journal of Assisted Reproduction and Genetics, Journal Year: 2018, Volume and Issue: 36(2), P. 189 - 198
Published: Oct. 25, 2018
Language: Английский
Citations
127
Journal of Controlled Release, Journal Year: 2019, Volume and Issue: 318, P. 25 - 37
Published: Dec. 9, 2019
Language: Английский
Citations
126
American Journal of Reproductive Immunology, Journal Year: 2020, Volume and Issue: 85(2)
Published: Oct. 29, 2020
Abstract Extracellular vesicles (EVs) are heterogeneous membranous secreted by every cell type and offer significant potential in therapy diagnostics. Differential ultracentrifugation is the gold standard for EV isolation, although other techniques including, polyethylene glycol (PEG) precipitation, immunoprecipitation, size exclusion chromatography, immuno‐isolation approaches common. Purified EVs can be characterized based on their physical characteristics, biochemical composition, or of origin. For concentration measurement, nanoparticle tracking analysis (NTA), dynamic light scattering (DLS), electron microscopy commonly employed methods. Biochemical analyses typically performed using flow cytometry, immunoblotting, proteomic investigation. Based tissue origin, have specific markers that used to isolate purify cell‐associated an affinity selection approach. Despite existence several methods isolation characterization, major limitations associated with each method hinder progress field. Evolving concepts biology possess great promise better characterization leading a insight biological function immense clinical implications. In this review, we discuss recent advancements approaches.
Language: Английский
Citations
117
Scientific Reports, Journal Year: 2019, Volume and Issue: 9(1)
Published: Jan. 24, 2019
Abstract Endocrine factors and signals of fetal organ maturation are reported determinants birth timing. To test the hypothesis that paracrine signaling by exosomes key regulators parturition, maternal plasma from CD-1 mice were isolated characterized throughout gestation biological pathways associated with differentially-expressed cargo proteins determined. Results indicate shape size remained constant gestation; however, a progressive increase in quantity carrying inflammatory mediators was observed day (E)5 to E19. In addition, effects late-gestation (E18) derived feto-maternal uterine tissues on parturition Intraperitoneal injection E18 into E15 localized reproductive tract intrauterine compartments. Compared controls delivered at term, preterm occurred exosome-treated preceded increased E17 cervix, uterus, membranes but not placenta. This effect injected early-gestation (E9) exosomes. study provides evidence function as labor delivery.
Language: Английский
Citations
109
Biomedicine & Pharmacotherapy, Journal Year: 2022, Volume and Issue: 148, P. 112752 - 112752
Published: Feb. 24, 2022
Exosomes are nano-sized structures that found in semen, epididymal –fluid, endometrium, as well follicular fluid. They responsible for transporting bioactive cargo- proteins, lipids, and nucleic acids. have been proven to influence processes both female male reproductive systems, including gametogenesis, acrosomal reaction, sperm capacitation, embryo implantation the endometrium. made of same particles cells they come from secreted by normal pathological cells. Therefore, exosomes can reflect physiological state Moreover, due transportation biomolecules, participate intercellular communication be used biomarkers many diseases, ovarian, endometrial prostate cancer. Identification could contribute a better understanding genital dysfunction fertility disorders.
Language: Английский
Citations
52
Frontiers in Endocrinology, Journal Year: 2022, Volume and Issue: 13
Published: Jan. 28, 2022
Extracellular vesicles (EVs) are membrane-coating nanoparticles derived from cells. The effect of cell-to-cell communication mediated by EVs has been investigated in different fields physio-logical as well pathological process recent years. Reproduction, regarded a definitive characteristic organisms, focus both animal and medical sciences. It is agreed that implantation critical event during early pregnancy viviparous animals, proper essential for the establishment maintenance normal pregnancy. However, successful requires synchronized development uterus embryo, therefore, which opportune regulation necessary. This review focuses on progression studies reveal role pregnancy, especially implantation. Based current evidence, produced exist environment proved origins such endometrium have positive influences embryo With their cargos proteins nucleic acids (especially microRNAs), exert effects including information transportation, immune stimulation gene expression.
Language: Английский
Citations
47
Journal of Animal Science and Biotechnology/Journal of animal science and biotechnology, Journal Year: 2022, Volume and Issue: 13(1)
Published: June 10, 2022
Extracellular vesicles (EVs) are nanosized membrane-enclosed compartments that serve as messengers in cell-to-cell communication, both normal physiology and pathological conditions. EVs can transfer functional proteins genetic information to alter the phenotype function of recipient cells, which undergo different changes positively affect their structural integrity. Biological fluids enriched with several subpopulations EVs, including exosomes, microvesicles (MVs), apoptotic bodies carrying cargoes, such lipids, proteins, nucleic acids. associated reproductive system actively involved regulation physiological events, gamete maturation, fertilization, embryo fetal development. influence follicle development, oocyte production, endometrial-conceptus communication. loaded cargoes used diagnose various diseases, pregnancy disorders; however, these dependent on type cell origin characteristics. EV-derived microRNAs (miRNAs) placenta regulate inflammatory responses trophoblast invasion through intercellular delivery placental microenvironment. This review presents evidence regarding types extracellular vesicles, general aspects isolation, purification, characterization particularly from embryos. Further, we discuss mediators biology, effects placentation disorders, role animal reproduction, male system, mother cross-communication. In addition, emphasize implantation therapeutic medicine. Finally, future perspectives biology.
Language: Английский
Citations
41
Materials Today Bio, Journal Year: 2023, Volume and Issue: 19, P. 100608 - 100608
Published: March 11, 2023
Exosomes are a subtype of extracellular vesicles (EVs) with size range between 30 and 150 nm, which can be released by the majority cell types circulate in body fluid. They function as long-distance cell-to-cell communication mechanism that modulates gene expression profile fate target cells. Increasing evidence has indicated exosomes' central role regulating various complex reproductive processes. However, to our knowledge, review focally vividly describes exosomes development is still lacking. This highlights knowledge about contribution early mammalian reproduction, such gametogenesis, fertilization, embryonic development, implantation, placentation pregnancy. The discussion primarily drawn from literature pertaining lineage emphasis on roles human reproduction laboratory livestock models.
Language: Английский
Citations
34
Journal of Animal Science and Biotechnology/Journal of animal science and biotechnology, Journal Year: 2024, Volume and Issue: 15(1)
Published: April 4, 2024
Abstract Background Extracellular vesicles (EVs) present in oviductal (OF) and uterine fluid (UF) have been shown to enhance bovine embryo quality during vitro culture by reducing lipid contents modulating metabolism-related genes (LMGs), while also influencing cell proliferation, suggesting their involvement on the regulation of different biological pathways. The signaling pathways related differentiation, metabolism is crucial for early development can determine success or failure pregnancy. Bioactive molecules within EVs maternal reproductive fluids, such as microRNAs (miRNAs), may contribute this regulatory process they modulate gene expression through post-transcriptional mechanisms. Results This study evaluated miRNA cargo OF-EVs from luteal phase UF-EVs mid-luteal phase, coinciding with transit oviduct uterus vivo, its possible influence LMGs development. A total 333 miRNAs were detected, 11 exclusive OF, 59 UF, 263 common between both groups. From 20 differentially expressed miRNAs, 19 up-regulated (bta-miR-134, bta-miR-151-3p, bta-miR-155, bta-miR-188, bta-miR-181b, bta-miR-181d, bta-miR-224, bta-miR-23b-3p, bta-miR-24-3p, bta-miR-27a-3p, bta-miR-29a, bta-miR-324, bta-miR-326, bta-miR-345-3p, bta-miR-410, bta-miR-652, bta-miR-677, bta-miR-873 bta-miR-708) one (bta-miR-148b) OF-EVs. These predicted several Wnt, Hippo, MAPK, degradation. Differences found reflect environments meet changing needs embryo. Additionally, be involved, particularly uterus, metabolism, immune system, implantation. Conclusions Our suggests that OF- could quality, providing insights into intricate maternal-embryonic communication might involved response, implantation
Language: Английский
Citations
12