Exosomes repairment for sciatic nerve injury: a cell-free therapy DOI Creative Commons

Guangda Xin,

Xueyan Liu, Xiaodi Fan

et al.

Stem Cell Research & Therapy, Journal Year: 2024, Volume and Issue: 15(1)

Published: July 18, 2024

Abstract Sciatic nerve injury (SNI) is a common type of peripheral typically resulting from trauma, such as contusion, sharp force injuries, drug injections, pelvic fractures, or hip dislocations. It leads to both sensory and motor dysfunctions, characterized by pain, numbness, loss sensation, muscle atrophy, reduced tone, limb paralysis. These symptoms can significantly diminish patient’s quality life. Following SNI, Wallerian degeneration occurs, which activates various signaling pathways, inflammatory factors, epigenetic regulators. Despite the availability several surgical nonsurgical treatments, their effectiveness remains suboptimal. Exosomes are extracellular vesicles with diameters ranging 30 150 nm, originating endoplasmic reticulum. They play crucial role in facilitating intercellular communication have emerged highly promising vehicles for delivery. Increasing evidence supports significant potential exosomes repairing SNI. This review delves into pathological progression techniques generating exosomes, molecular mechanisms behind SNI recovery combining other approaches repair, changes future outlook utilizing recovery.

Language: Английский

Advances in the application of platelet-rich plasma in peripheral nerve injuries DOI Creative Commons

Xiao-Yu Dou,

Min An

Anesthesiology and Perioperative Science, Journal Year: 2025, Volume and Issue: 3(2)

Published: April 18, 2025

Abstract Peripheral nerve injury (PNI) can occur after various types of trauma; these are common clinical injuries. These injuries often result in chronic conditions such as neuropathic pain and permanent disabilities, severely affecting quality life work. Achieving optimal repair injured peripheral nerves remains a focal point research. In recent years, platelet-rich plasma (PRP) has been increasingly applied settings due to associated high concentration growth factors that promote tissue repair. Here we review advances PNI the prospect using autologous PRP treatment paradigm.

Language: Английский

Citations

0
PBMC-mediated modulation of macrophage polarization in RAW264.7 cells through STAT1/STAT6 signaling cascades DOI Creative Commons
Wenbo Zhang,

Zuxiang Chen,

Zhen Liu

et al.

International Immunopharmacology, Journal Year: 2024, Volume and Issue: 138, P. 112651 - 112651

Published: July 9, 2024

Peripheral blood mononuclear cells (PBMC), sourced autologously, offer numerous advantages when procured: easier acquisition process, no in vitro amplification needed, decreased intervention and overall increased acceptability make PBMC an attractive candidate for cell therapy treatment. However, the exact mechanism by which treat diseases remains poorly understood. Immune imbalance is pathological basis of many diseases, with macrophages playing a crucial role this process. research on mechanisms regulating scarce. This study employed co-culture model RAW264.7 to explore macrophages. The results showed that co-culturing led expression inflammatory cytokines anti-inflammatory or culture supernatant. Additionally, pro-inflammatory, tissue matrix-degrading M1 decreased, while anti-inflammatory, matrix-synthesizing, regenerative M2 both monocytes within PBMC. Moreover, co-cultured exhibited significantly p-STAT1/STAT1 ratio, p-STAT6/STAT6 ratio increased. suggests may inhibit macrophage polarization blocking STAT1 signaling cascades promote through activation STAT6 cascades. Overall, sheds light it was found differentiated into presence finding provides experimental evidence use treating especially macrophage-depleting such as osteoarthritis.

Language: Английский

Citations

3
Basic Science and Principles of Stem Cells, Platelet‐Rich Plasma, and Exosomes DOI

Elliott Lyles,

Carol Stegura,

Luke Broughton

et al.

Dermatological Reviews, Journal Year: 2024, Volume and Issue: 5(4)

Published: Aug. 1, 2024

ABSTRACT Background Regenerative medicine seeks to harness the natural healing abilities of body and has scope for use in treatment dermatologic conditions. Stem cell therapy, platelet‐rich plasma (PRP), exosomes are emerging as key components regenerative medicine, particularly skin rejuvenation repair. Objectives The goal this article is review basic science behind function stem cells, PRP, medicine. Methods A literature was conducted using PubMed Google Scholar, focusing on regarding structure, origin, mechanisms action, isolation exosomes. Results evaluation found that cells facilitate regeneration by modulating inflammatory milieu. PRP shown have positive effects repairing elasticity improving scars through release growth factors. Exosomes were enhance fibroblast proliferation collagen synthesis. Conclusions each show render them useful Future research needed further elucidate their standardize procedures, optimize potential clinical application.

Language: Английский

Citations

3
Umbilical cord mesenchymal stem cells combined with autologous platelet-rich plasma for lower extremity venous ulcers: A case report and literature review DOI Creative Commons

Linlin Jiao,

Jing Nie,

Lian Duan

et al.

Medicine, Journal Year: 2024, Volume and Issue: 103(45), P. e40433 - e40433

Published: Nov. 8, 2024

Rationale: Nonhealing ulcers are difficult to manage because they deviate from the normal wound healing process. Conventional therapy cannot achieve satisfactory therapeutic effects. To verify effectiveness of combined treatment with human umbilical cord mesenchymal stem cells (hUMSCs) and platelet-rich plasma (PRP) for nonhealing ulcers, we studied a patient left lower limb venous ulcer (LEVU) treated injection therapy. Patient concerns: We present case LEVU who has not healed long period time (up 1 year). Diagnoses: was diagnosed clinical symptoms. Interventions: The hUMSCs plus PRP were injected into edge base (1 µL cells/cm 2 surface), 0.5 mL at each point, distance approximately 3 cm between points. point determined according extent involvement. Outcomes: Seven days after hUMSC + application, area decreased by nearly 50%. had almost completely day 62, no serious treatment-related toxic side effects observed. Lessons: can improve through re-epithelialization, increased angiogenesis, granulation tissue formation. also been suggested promote secretion various nutritional factors. combination mutually reinforcing effect, which may > effect. Therefore, be safe effective option LEVU.

Language: Английский

Citations

1
Exosomes repairment for sciatic nerve injury: a cell-free therapy DOI Creative Commons

Guangda Xin,

Xueyan Liu, Xiaodi Fan

et al.

Stem Cell Research & Therapy, Journal Year: 2024, Volume and Issue: 15(1)

Published: July 18, 2024

Abstract Sciatic nerve injury (SNI) is a common type of peripheral typically resulting from trauma, such as contusion, sharp force injuries, drug injections, pelvic fractures, or hip dislocations. It leads to both sensory and motor dysfunctions, characterized by pain, numbness, loss sensation, muscle atrophy, reduced tone, limb paralysis. These symptoms can significantly diminish patient’s quality life. Following SNI, Wallerian degeneration occurs, which activates various signaling pathways, inflammatory factors, epigenetic regulators. Despite the availability several surgical nonsurgical treatments, their effectiveness remains suboptimal. Exosomes are extracellular vesicles with diameters ranging 30 150 nm, originating endoplasmic reticulum. They play crucial role in facilitating intercellular communication have emerged highly promising vehicles for delivery. Increasing evidence supports significant potential exosomes repairing SNI. This review delves into pathological progression techniques generating exosomes, molecular mechanisms behind SNI recovery combining other approaches repair, changes future outlook utilizing recovery.

Language: Английский

Citations

0