The Interplay between Finasteride-Induced Androgen Imbalance, Endoplasmic Reticulum Stress, Oxidative Stress, and Liver Disorders in Paternal and Filial Generation DOI Creative Commons
Sylwia Rzeszotek, Agnieszka Kolasa, Anna Pilutin

et al.

Biomedicines, Journal Year: 2022, Volume and Issue: 10(11), P. 2725 - 2725

Published: Oct. 27, 2022

Finasteride (Fin) causes androgen imbalance by inhibiting the conversion of testosterone (T) to its more active metabolite, dihydrotestosterone (DHT). Androgen receptors (AR) are present (e.g., in hepatocytes), which have well-developed endoplasmic reticulum (ERet). Cellular protein quality control is carried out ERet two paths: (i) unfolded response (UPR) and/or (ii) associated degradation (ERAD). under continuous stress can generate changes UPR and direct cell on pathway life or death. It has been demonstrated that genes involved among controlled androgens some tissues. Oxidative also one factors affecting functions regulators antioxidant enzyme activity. In this paper, we discuss/analyze a possible relationship between paternal generation with liver disorders both filial generation. our rat model, hyperglycemia subsequent higher accumulation hepatic glycogen were observed all obtained from females fertilized Fin-treated males (F1:Fin). Importantly, encoding enzymes glucose metabolism previously recognized targets.

Language: Английский

An in vitro testicular organoid model for the study of testis morphogenesis, somatic cell maturation, endocrine function, and toxicological assessment of endocrine disruptors DOI Creative Commons
Tat-Chuan Cham, Fahar Ibtisham, Ahmad Al‐Dissi

et al.

Reproductive Toxicology, Journal Year: 2024, Volume and Issue: 128, P. 108645 - 108645

Published: June 17, 2024

Male reproductive capacity has fallen considerably in recent decades; addition, the incidence of testicular cancer increased many developed countries. The cause this phenomenon is unknown, but environmental toxicants are considered a major contributing factor. To study potential toxicants, robust vitro testis models needed. We have recently established porcine organoid system with high resemblance to architectures innate tissue. Here, we further investigated morphogenesis, cell maturation, and endocrine function organoids. also challenged abiraterone, steroidogenic inhibitor, validate its suitability as an platform for toxicology tests. Our results showed that cells organoids reorganize into cordal structures, relative areas increase over time culture. Moreover, diameters numbers per cross-section structures time. Interestingly, Sertoli gradually underwent maturational changes by showing expression androgen receptors, decreased anti-müllerian hormone, formation blood-testis barrier. Next, confirmed respond hormonal stimulation release multiple sex hormones, including testosterone, estradiol, progesterone. Finally, production testosterone estradiol can be inhibited response inhibitor. Taken together, our provides promising male studies on somatic production.

Language: Английский

Citations

5
BaP/BPDE suppresses human trophoblast cell migration/invasion and induces unexplained miscarriage by up-regulating a novel lnc-HZ11 in extracellular vesicles: An intercellular study DOI Creative Commons

Chenyang Mi,

Weina Chen, Ying Zhang

et al.

Environment International, Journal Year: 2024, Volume and Issue: 188, P. 108750 - 108750

Published: May 21, 2024

Extracellular vesicles (EVs) mediate the intercellular crosstalk by transferring functional cargoes. Recently, we have discovered that BaP/BPDE exposure suppresses trophoblast cell migration/invasion and induces miscarriage, which are also regulate lncRNAs at intracelluar levels. However, EVs-mediated regulatory mechanisms completely unexplored. Specifically, whether EVs might transfer BPDE-induced toxic lncRNA to fresh recipient cells suppress their further induce miscarriage is unknown. In this study, find BPDE up-regulates a novel lnc-HZ11, EGR1/NF-κB/CXCL12 pathway of cells. Intercellular studies show EV-HZ11 (lnc-HZ11 in EVs), highly expressed BPDE-exposed donor cells, lnc-HZ11 through EVs. Analysis villous tissues collected from UM (unexplained miscarriage) patients HC (healthy control) group shows levels BPDE-DNA adducts, or EV-lnc-HZ11, all associated with miscarriage. Mouse assays BaP lnc-Hz11 EV-Hz11, Egr1/Nf-κb/Cxcl12 pathway, eventually Knockdown injecting EV-AS-Hz11 could effectively alleviate BaP-exposed mice. Furthermore, serum samples well predict risk Collectively, study not only discovers EVs-HZ11-mediated but provides new approach for treatment against unexplained EV-HZ11.

Language: Английский

Citations

4
Effect of in vitro exposure of first-line antiretrovirals on healthy human spermatozoa on kinematics and motility DOI Creative Commons

S. Pinto,

Natalie Aneck-Hahn

International Urology and Nephrology, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 3, 2025

Abstract Purpose Contemporary antiretroviral (ARV) medications are used by millions of men for HIV treatment worldwide. Limited data exist on their direct effect sperm motility. This pilot study hypothesizes that in vitro exposure to ARVs will reduce kinematic and motility parameter values. Methods laboratory-based experimental analyzed kinematics after the Dolutegravir, Tenofovir, Emtricitabine, individually combination. Each participant (n = 23) served as control. The Microptic SCA® Computer Assisted Sperm Analysis (CASA) system, Barcelona, Spain was generate quantitative Straight-line velocity (VSL), Straightness index (STR), Linearity Index (LIN), Beat cross frequency (BCF), oscillation (WOB). Results VSL, STR, LIN, WOB non-progressive (grade c) spermatozoa were significantly decreased ARV treatment. BCF medium progressive population b) increased 90 min Tenofovir arm, a significant decrease proportion grade b recorded at all arms when compared control arm. No impaired observed within first 30 exposure. Conclusion Pharmacovigilance is healthcare emergency fast-changing world newer drugs leaves clinicians vulnerable. They must prescribe whose long-term somatic germline adverse effects not fully understood. Guidelines changing faster than we can monitor side effects. Despite Dolutegravir being only mainstream integrase inhibitor first-line South Africa five years, its replacement, Cabotegravir, already launched. More research this field required, especially commonly prescribed drugs. preliminary concludes current patients HIV-negative pre-exposure prophylaxis (PrEP) alter kinematics. Further with larger sample size warranted quantify impact human fertility, addressing limitations study, before comprehensive conclusion male fertility be drawn. Of particular importance would reactive oxygen species levels semen DNA fragmentation.

Language: Английский

Citations

0
Bisphenol F induces spermatogenic cell ferroptosis via FTO-mediated m6A regulation of FTH1 DOI

Shimeng Zhou,

Yu Shi,

Jiang-ying Li

et al.

Free Radical Biology and Medicine, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 1, 2025

Language: Английский

Citations

0
Performance of ultrasound in detecting fetal hypospadias during pregnancy: a pooled analysis DOI Creative Commons
Qiang Zhang, Hongsong Chen, Chong Wang

et al.

EClinicalMedicine, Journal Year: 2025, Volume and Issue: 81, P. 103091 - 103091

Published: Feb. 1, 2025

Language: Английский

Citations

0
Current perspectives in hypospadias research: A scoping review of articles published in 2021 (Review) DOI Open Access

Horea Gozar,

Zsolt Bara,

Emilia Dicu

et al.

Experimental and Therapeutic Medicine, Journal Year: 2023, Volume and Issue: 25(5)

Published: March 23, 2023

Hundreds of papers are written about hypospadias every year referring to all aspects the pathology, being one most common congenital malformations. The present study conducted a scoping review articles published in 2021 main issues and summarize current perspectives achievements field. It searched for keyword 'hypospadias' three popular databases (PubMed, Scopus Web Science). After analysis publications, they were categorized into different domains. was performed respecting Preferred Reporting Items Systematic reviews Meta‑Analyses extension Scoping Reviews (PRISMA ScR) guidelines. A total 284 included. These 142 journals. accessed Journal Paediatric Urology with 54 articles. identified domains related surgical techniques, postoperative care, complications, anesthesia, anatomical factors, genetics, environmental endocrinology, associated malformations, questionnaires recommendations, management, biological materials, animal models, retrospective studies centers, social media, bibliometrics, small gestational age, neoplasm, or fertility. Promising modifications existing techniques presented improved outcomes both proximal distal types hypospadias. Relevant etiological, also genetic factors clarified. Aspects peri‑ management antibiotherapy, analgesia, dressing future use novel bioengineering agents prevent, reduce treat occurring complications discussed.

Language: Английский

Citations

8
Human development and reproduction in space—a European perspective DOI Creative Commons
Varsha Jain, Susana M. Chuva de Sousa Lopes,

Mohammed Abderrafi Benotmane

et al.

npj Microgravity, Journal Year: 2023, Volume and Issue: 9(1)

Published: March 27, 2023

Abstract This review summarises key aspects of the first reproductive and developmental systems Science Community White Paper, supported by European Space Agency (ESA). Current knowledge regarding human development reproduction in space is mapped to roadmap. It acknowledges that sex gender have implications on all physiological systems, however, identity falls outside scope document included white paper collection ESA. The ESA SciSpacE papers functions aim reflect travel male female including hypothalamic-pituitary-gonadal (HPG) hormone axis, considerations for conception, gestation birth. Finally, parallels are drawn as how this may impact society a whole Earth.

Language: Английский

Citations

8
Disruptores endocrinos y enfermedades endocrinológicas en niños y adolescentes DOI Creative Commons
L. Rodríguez, Camila Céspedes, Catalina Forero

et al.

Revista Colombiana de Endocrinología Diabetes & Metabolismo, Journal Year: 2024, Volume and Issue: 11(2)

Published: June 17, 2024

Contexto: la influencia del contacto con disruptores endocrinos, tanto en el periodo prenatal como posnatal, ha generado preocupación, debido a su posible relación desarrollo de enfermedades endocrinológicas población pediátrica. Objetivo: recopilar evidencia científica respecto presentación niños y adolescentes consecuencia exposición endocrinos. Metodología: esta revisión narrativa se examinó literatura publicada sobre este tema mediante utilización bases datos electrónicas búsquedas manuales revistas especializadas. En total seleccionaron 33 artículos. Resultados: encontró entre estas sustancias no transmisibles, tales obesidad, diabetes, síndrome metabólico, trastornos crecimiento desarrollo, alteraciones función tiroidea. Asimismo, comprobado que dichos compuestos puede generar repercusiones largo plazo endocrino reproductivo. Conclusión: hay realizar investigaciones adicionales para abordar las brechas conocimiento actual establecer una base robusta respalde toma decisiones salud pública.

Citations

2
Decline of Sperm Quality over the Last Two Decades in the South of Europe: A Retrospective Study in Infertile Patients DOI Creative Commons
E Grau,

Judith Lleberia,

Laura Costa

et al.

Biology, Journal Year: 2022, Volume and Issue: 12(1), P. 70 - 70

Published: Dec. 30, 2022

Semen quality has a direct relation to male fertility. Whether sperm variables in humans have decreased over the last years is still uncertain, with some studies showing decline and others reporting no changes. In this regard, previous research suggested that lifestyle environmental conditions may contribute variability, calling for regional studies. The present work retrospective, unicentric study includes semen samples analyzed between 1997 2017 at Parc Taulí Hospital (Barcelona metropolitan area). First, multivariate analysis including age as confounding factor showed statistically significant decrease volume, pH, progressive motility, morphology total motile time. Contrarily, variation count or concentration was observed. Mean reductions per year were −0.02 mL −0.57% progressively −0.72% normal morphology. Interestingly, average annual temperature registered by Spanish Meteorology Agency negatively correlated (Rs = −0.642; p 0.002 Rs −0.435; 0.049, respectively). conclusion, based on infertile patients from Barcelona area found motility morphology, without effects count. Changes appeared be associated decline, but further are needed address mechanisms linked observed variations.

Language: Английский

Citations

8
Prenatal DEHP exposure induces lifelong testicular toxicity by continuously interfering with steroidogenic gene expression DOI Open Access

Qigen Xie,

Haiming Cao, Hanchao Liu

et al.

Translational Andrology and Urology, Journal Year: 2024, Volume and Issue: 13(3), P. 369 - 382

Published: March 1, 2024

Epidemiologic studies suggested the association between prenatal di-(2-ethylhexyl) phthalate (DEHP) exposure and disorders of sex development (DSD), adult male disorders, reproductive aging. Inhibiting testosterone synthesis by interfering with steroidogenic gene expression induces testicular toxicity, however, whether DEHP toxicity through this mechanism remains uncertain.

Language: Английский

Citations

1