Extracellular vesicle-derived silk fibroin nanoparticles loaded with MFGE8 accelerate skin ulcer healing by targeting the vascular endothelial cells DOI Creative Commons
Liwen Luo, Hong Yu Zhang, Shiyu Zhang

et al.

Journal of Nanobiotechnology, Journal Year: 2023, Volume and Issue: 21(1)

Published: Nov. 29, 2023

Reduced supplies of oxygen and nutrients caused by vascular injury lead to difficult-to-heal pressure ulcers (PU) in clinical practice. Rapid repair the skin wound is key resolution this challenge, but measures are still limited. We described beneficial effects extracellular vesicle-derived silk fibroin nanoparticles (NPs) loaded with milk fat globule EGF factor 8 (MFGE8) on accelerating blood vessel PU healing targeting CD13 endothelial cells (VECs).CD13, specific protein NGR, MFGE8, an inhibitor ferroptosis, were detected VECs tissues. Then, NPs synthesized via fibroin, MFGE8-coated (NPs@MFGE8) assembled loading purified MFGE8 produced Chinese hamster ovary cells. Lentivirus was used over-express obtained MFGE8-engineered vesicles (EVs-MFGE8) secreted these VECs. The inhibitory effect EVs-MFGE8 or NPs@MFGE8 ferroptosis vitro. NGR peptide cross-linked into NGR-NPs@MFGE8. Collagen synthesize fibroin/collagen hydrogel. After being NGR-NPs@MFGE8, hydrogel sustained-release carrier investigate vivo.MFGE8 decreased, increased Similar inhibiting could inhibit mitochondrial autophagy-induced Compared hydrogels NPs@MFGE8, NGR-NPs@MFGE8 consistently released VECs, thereby autophagy hypoxia effectively rats.The great significance use as a dressing tissues, preventing formation promoting healing.

Language: Английский

Designing hydrogel for application in spinal surgery DOI Creative Commons

Rongpeng Dong,

Shuang Zheng, Xueliang Cheng

et al.

Materials Today Bio, Journal Year: 2025, Volume and Issue: 31, P. 101536 - 101536

Published: Feb. 3, 2025

Language: Английский

Citations

1
A robust visualized sericin hydrogel dressing with excellent antioxidative and antimicrobial activities facilitates diabetic wound healing DOI
Xingyue Wang, Susu Wang,

Akoumay Tehoungue

et al.

Journal of the mechanical behavior of biomedical materials/Journal of mechanical behavior of biomedical materials, Journal Year: 2025, Volume and Issue: 165, P. 106927 - 106927

Published: Feb. 7, 2025

Language: Английский

Citations

1
Exosome-loaded methacrylated silk fibroin hydrogel delays intervertebral disc degeneration by DKK2-mediated mitochondrial unfolded protein response DOI
Xiao Lu, Zhidi Lin,

Liujingzhou Li

et al.

Chemical Engineering Journal, Journal Year: 2025, Volume and Issue: unknown, P. 162191 - 162191

Published: March 1, 2025

Language: Английский

Citations

1
Recent Advances in Managing Spinal Intervertebral Discs Degeneration DOI Open Access
Bogdan Costăchescu, Adelina-Gabriela Niculescu, Raluca Ioana Teleanu

et al.

International Journal of Molecular Sciences, Journal Year: 2022, Volume and Issue: 23(12), P. 6460 - 6460

Published: June 9, 2022

Low back pain (LBP) represents a frequent and debilitating condition affecting large part of the global population posing worldwide health economic burden. The major cause LBP is intervertebral disc degeneration (IDD), complex disease that can further aggravate give rise to severe spine problems. As most current treatments for IDD either only alleviate associated symptoms or expose patients risk intraoperative postoperative complications, there pressing need develop better therapeutic strategies. In this respect, present paper first describes pathogenesis etiology set framework what has be combated restore normal state discs (IVDs), then elaborates on recent advances in managing IDD. Specifically, are reviewed bioactive compounds growth factors have shown promising potential against underlying IDD, cell-based therapies IVD regeneration, biomimetic artificial IVDs, several other emerging options (e.g., exosomes, RNA approaches, intelligence).

Language: Английский

Citations

34
Injectable thermo-sensitive hydrogel containing ADSC-derived exosomes for the treatment of cavernous nerve injury DOI
Shuai Liu, Ruiyu Li,

Keke Dou

et al.

Carbohydrate Polymers, Journal Year: 2022, Volume and Issue: 300, P. 120226 - 120226

Published: Oct. 14, 2022

Language: Английский

Citations

31
Degenerated nucleus pulposus cells derived exosome carrying miR-27a-3p aggravates intervertebral disc degeneration by inducing M1 polarization of macrophages DOI Creative Commons
Xin Zhao, Zhen Sun,

Benchi Xu

et al.

Journal of Nanobiotechnology, Journal Year: 2023, Volume and Issue: 21(1)

Published: Sept. 4, 2023

Intervertebral disc degeneration (IVDD) is a major contributor to spinal disorders. Previous studies have indicated that the infiltration of immunocytes, specifically macrophages, plays crucial role in advancement IVDD. Exosomes (exo) are believed play significant intercellular communication. This study aims investigate exosomes derived from degenerated nucleus pulposus (dNPc) process macrophages M1 polarization.Nucleus (NP) tissue and cells (NPc) were collected patients with intervertebral idiopathic scoliosis. Immunohistochemistry analysis was performed determine number NP tissue. Subsequently, (dNPc-exo) non-degenerated (nNPc-exo) co-cultured M0 which induced THP-1 cells. The phenotype assessed using western blot, flow cytometry, immunofluorescence staining, qRT-PCR. RNA-sequencing conducted examine expression levels microRNAs dNPc-exo nNPc-exo groups, qRT-PCR effect pf different microRNA induce macrophage polarization. Furthermore, blot employed demonstrate regulatory carried by on downstream target signaling pathways macrophages. Finally, an animal model IVDD utilized impact inducing polarization its process.In this study, we observed increase as (IVD) degraded. Additionally, discovered dNPc releases could promote towards phenotype. Notably, through identified miR-27a-3p highly expressed miRNA group, significantly influences induction And then, has ability transport PPARγ/NFκB/PI3K/AKT pathway, thereby influencing We experiments rat carrying actually exacerbated degradation IVD.In conclusion, our findings highlight provide basis for further investigation into mechanism potential exosome-based therapy.

Language: Английский

Citations

18
Research progress in decellularized extracellular matrix hydrogels for intervertebral disc degeneration DOI
Bing Peng, Lilong Du, Tongxing Zhang

et al.

Biomaterials Science, Journal Year: 2023, Volume and Issue: 11(6), P. 1981 - 1993

Published: Jan. 1, 2023

This review describes the classification of hydrogels, methods production decellularised extracellular matrix (dECM) and gel formation. Finally, role dECM hydrogels in treatment intervertebral disc degeneration is summarized.

Language: Английский

Citations

17
FSTL1 Accelerates Nucleus Pulposus Cell Senescence and Intervertebral Disc Degeneration Through TLR4/NF-κB Pathway DOI

Xu Yan,

Jing-Yu Ding,

Renjie Zhang

et al.

Inflammation, Journal Year: 2024, Volume and Issue: 47(4), P. 1229 - 1247

Published: Feb. 6, 2024

Language: Английский

Citations

8
Bioinspired engineering ADSC nanovesicles thermosensitive hydrogel enhance autophagy of dermal papilla cells for androgenetic alopecia treatment DOI Creative Commons
Jiachao Xiong,

Zhixiao Liu,

Lingling Jia

et al.

Bioactive Materials, Journal Year: 2024, Volume and Issue: 36, P. 112 - 125

Published: March 1, 2024

Androgenic alopecia (AGA) is a highly prevalent form of non-scarring but lacks effective treatments. Stem cell exosomes have similar repair effects to stem cells, suffer from the drawbacks high cost and low yield yet. Cell-derived nanovesicles acquired through mechanical extrusion exhibit favorable biomimetic properties exosomes, enabling them efficiently encapsulate substantial quantities therapeutic proteins. In this study, we observed that JAM-A, an adhesion protein, resulted in significantly increased resilience dermal papilla cells snap structures against damage caused by dihydrotestosterone macrophages, thereby facilitating process hair regrowth cases AGA. Consequently, adipose-derived were modified overexpress JAM-A produce engineered overexpressing (JAM-AOE@NV). The incorporation JAM-AOE@NV into thermosensitive hydrogel matrix (JAM-AOE@NV Gel) effectively addresses limitations associated with short half-life JAM-AOE@NV, achievement sustained-release profile for JAM-AOE@NV. physicochemical characteristics Gel analyzed assessed its efficacy promoting vivo vitro. Gel, thus, presents novel approach theoretical framework treatment diseases

Language: Английский

Citations

8
Decellularized nucleus pulposus matrix/chitosan hybrid hydrogel combined with nucleus pulposus stem cells and GDF5-loaded microspheres for intervertebral disc degeneration prevention DOI Creative Commons
Tao Ma, Chen Liu,

Quanlai Zhao

et al.

Molecular Medicine, Journal Year: 2024, Volume and Issue: 30(1)

Published: Jan. 10, 2024

Abstract Background Intervertebral disc degeneration (IDD) is considered an important pathological basis for spinal degenerative diseases. Tissue engineering a powerful therapeutic strategy that can effectively restore the normal biological properties of units. In this study, hydrogels loaded with growth/differentiation factor 5 (GDF5) and stem cells were combined to provide effective nucleus pulposus regeneration. Methods Nucleus (NPSCs) obtained by low-density inoculation culture, their cell characteristics verified flow cytometry tri-lineage-induced differentiation experiment. A decellularized matrix (DNPM) chitosan hybrid hydrogel was prepared, GDF5-loaded poly(lactic-co-glycolic acid) (PLGA) microspheres incorporated into obtain composite microspheres. Taking bone marrow mesenchymal (BMSCs) as reference, effect on chondrogenic NPSCs evaluated. model intervertebral induced acupuncture tail rats constructed, repair IDD observed. Results Stem phenotype identification, stemness gene expression confirmed had similar those BMSCs. The rat DNPM good mechanical properties, sustainably released GDF5. grew normally in gradually expressed chondrocyte phenotype. Animal experiments showed promoted regeneration significantly better than Conclusion DNPM/chitosan promote pulposus-like preventIDD.

Language: Английский

Citations

7