Ultrahigh-throughput screening of Trichoderma reesei strains capable of carbon catabolite repression release and cellulase hyperproduction using a microfluidic droplet platform DOI

Xuan Chinh Luu,

Yosuke Shida, Yoshiyuki Suzuki

et al.

Bioscience Biotechnology and Biochemistry, Journal Year: 2023, Volume and Issue: 87(11), P. 1393 - 1406

Published: Aug. 8, 2023

ABSTRACT Trichoderma reesei is the most well-known cellulase producer in biorefinery industry. Its biosynthesis repressed by glucose via carbon catabolite repression (CCR), making CCR-releasing strains with hyperproduction desirable. Here, we employed a microfluidic droplet platform to culture and screen T. mutants capable of CCR release overproduction from extensive mutagenesis libraries. With 3 rounds, about 6.20 × 103 droplets were sorted population 1.51 106 period 4.4 h; 76 recovery screened on flask fermentation, 2 uptake retarded mutants, MG-9-3 MG-9-3-30, eventually isolated. We also generated hypercellulase producer, M-5, single round. The hyphal morphology molecular mechanisms analyzed. This versatile approach combined comprehensive understanding will provide innovative effective strategies for low-cost production.

Language: Английский

Bioresource Upgrade for Sustainable Energy, Environment, and Biomedicine DOI Creative Commons
Fanghua Li, Yiwei Li, Kostya S. Novoselov

et al.

Nano-Micro Letters, Journal Year: 2023, Volume and Issue: 15(1)

Published: Jan. 11, 2023

We conceptualize bioresource upgrade for sustainable energy, environment, and biomedicine with a focus on circular economy, sustainability, carbon neutrality using high availability low utilization biomass (HALUB). acme energy-efficient technologies energy material recovery applications. The of thermochemical conversion (TC), biochemical (BC), electrochemical (EC), photochemical (PTC) are summarized HALUB. Microalgal could contribute to biofuel HHV 35.72 MJ Kg

Language: Английский

Citations

55
Nature-inspired micropatterns DOI
Yunhua Wang, Guoxia Zheng, Nan Jiang

et al.

Nature Reviews Methods Primers, Journal Year: 2023, Volume and Issue: 3(1)

Published: Sept. 7, 2023

Language: Английский

Citations

47
A comprehensive review on the fundamental principles, innovative designs, and multidisciplinary applications of micromixers DOI
Wenbo Han, Wei Li, Hongpeng Zhang

et al.

Physics of Fluids, Journal Year: 2024, Volume and Issue: 36(10)

Published: Oct. 1, 2024

This paper comprehensively reviews the fundamental principles, innovative designs, and multidisciplinary applications of micromixers. First, it introduces principles fluid mixing in micromixers, including passive active mechanisms, flow characteristics fluids at microscale. Subsequently, focuses on design covering a variety such as obstacle structures, curved serpentine groove separation recombination topology optimization baffle analyzes effects different structures efficiency pressure drop. In addition, also studies magnetic field assistance, electric surface acoustic wave thermal effect driving modes efficiency. Finally, outlines micromixers fields biomedicine, chemical analysis, environmental monitoring control, new energy. review aims to provide comprehensive reference for research application promote their more fields.

Language: Английский

Citations

31
Minimum Minutes Machine-Learning Microfluidic Microbe Monitoring Method (M7) DOI
Ning Yang,

Wei Song,

Yi Xiao

et al.

ACS Nano, Journal Year: 2024, Volume and Issue: 18(6), P. 4862 - 4870

Published: Jan. 17, 2024

Frequent outbreaks of viral diseases have brought substantial negative impacts on society and the economy, they are very difficult to detect, as concentration aerosols in air is low composition complex. The traditional detection method manually collection re-detection, being cumbersome time-consuming. Here we propose a virus aerosol based microfluidic inertial separation spectroscopic analysis technology rapidly accurately detect particles air. chip designed principles laminar flow characteristics, resulting an average efficiency 95.99% for 2 μm particles. We build composite spectrometer platform capture spectral information dynamically. By employing machine-learning techniques, can classify different types entire experiment took less than 30 min compared with hours by PCR detection. Furthermore, our model achieves accuracy 97.87% identifying aerosols, which comparable results obtained from

Language: Английский

Citations

10
AI-driven development of high-performance solid-state hydrogen storage DOI Creative Commons
Guoqing Wang,

Zongmin Luo,

Halefom G. Desta

et al.

Energy Reviews, Journal Year: 2024, Volume and Issue: 4(1), P. 100106 - 100106

Published: Aug. 10, 2024

Energy drives the development of human civilization, and hydrogen energy is an inevitable choice under goal "global transition". As technology continues to advance, solid-state storage materials have attracted significant attention as efficient solution for storage. However, existing research methods, such experimental preparation theoretical calculations, are inefficient costly. Here, we summarize latest advancements high-throughput screening (HTS) machine learning (ML) materials. It elaborates on advantages HTS ML in rapid material screening, performance assessment prediction, so on. We place particular emphasis exploration analysis progress involving application various types Additionally, discuss integrating ML, emphasizing this comprehensive strategy field In realm storage, artificial intelligence plays a dual role. not only enhances efficiency but also offers novel tools future design development. This will aid discovery new-type high-performance materials, facilitate their commercialization practical application.

Language: Английский

Citations

8
Improving Plant Photosynthesis through Light-Harvesting Upconversion Nanoparticles DOI

Xiaokai Xu,

Rongxin Shen,

Luoqi Mo

et al.

ACS Nano, Journal Year: 2022, Volume and Issue: 16(11), P. 18027 - 18037

Published: Nov. 7, 2022

Nanotechnology is considered as an emerging effective means to augment plant photosynthesis. However, there still a lot of work be done in this field. Here, we applied the upconversion nanoparticles (UCNPs) on lettuce leaves and found that UCNPs were able transport into body colocalize with chloroplasts. It was proved could harvest near-infrared light sunlight increase electron transfer rate photosynthesis process, thus increasing rate. The gene expression analysis showed more than 90% upregulated. After spraying UCNP solution placing under for 1 week, wet/dry weight increased by 53.33% 45.71%, respectively. This nanoengineering light-harvesting may have great potential applications agriculture.

Language: Английский

Citations

22
The need for smart microalgal bioprospecting DOI Creative Commons
Joan Labara Tirado, Andrei Herdean, Peter J. Ralph

et al.

Natural Products and Bioprospecting, Journal Year: 2025, Volume and Issue: 15(1)

Published: Jan. 15, 2025

Abstract Microalgae’s adaptability and resilience to Earth’s diverse environments have evolved these photosynthetic microorganisms into a biotechnological source of industrially relevant physiological functions biometabolites. Despite this, microalgae-based industries only exploit handful species. This lack biodiversity hinders the expansion microalgal industry. Microalgal bioprospecting, searching for novel biological algal resources with new properties, remains low throughput time-consuming endeavour due inefficient workflows that rely on non-selective sampling, monoalgal culture status outdated, non-standardized characterization techniques. review will highlight importance bioprospecting critically explore commonly employed methodologies. We also current advances driving next generation smart focusing transdisciplinary methodologies potential enable high-throughput biodiscoveries. Images adapted from (Addicted04 in Wikipedia File: Australia globe (Australia centered).svg. 2014.; Jin et al. ACS Appl Bio Mater 4:5080–5089, 2021; Kim Microchim Acta 189:88, 2022; Tony Lab Chip 15, 19:3810–3810; Thermo Fisher Scientific INC. CTS Rotea Brochure). Graphical abstract

Language: Английский

Citations

0
Systematic review of peptide nanoparticles for improved diabetes outcomes: insights and opportunities DOI Creative Commons
Reuben Samson Dangana, Michael Ben Okon,

Ikuomola Emmanuel Orire

et al.

Discover Nano, Journal Year: 2025, Volume and Issue: 20(1)

Published: Feb. 17, 2025

This present study carried out a systematic review and meta-analysis of peptide nanoparticles in diabetes management for improved patient outcomes from 2014 to 2024. Different electronic databases, including PubMed, Scopus, Web Science, ResearchGate, Google Scholar, the Cochrane Library, were searched relevant literature using Medical Subject Headings (MeSH) boolean operators. A total 317 articles obtained include PUBMED (39), Scopus (215), ResearchGate (30), Scholar (25), Library (8). From these, 186 duplicate entries eliminated, while 76 dismissed some reasons. After scanning titles, abstracts, contents remaining 55 relevance, 22 eliminated. full-text screening inclusion/exclusion criteria, an additional 11 discarded, 4 excluded during data extraction phase. In end, seven (7) publications considered based on eligibility representing 2.22%. Results showed that sequential exclusion studies did not have significant impact effects glucose control, insulin delivery, bioavailability, efficacy, safety, management. Also, had positive improvement glycemic levels, glycated hemoglobin (HbA1C) overall outcomes. The concludes harbour potential improve through enhanced However, there is gap knowledge. Further research required understand long-term safety efficacy many enlisted nanoparticles. Additionally, future should explore wider range peptides proteins encapsulation, develop delivery systems larger conformationally diverse molecules, oral bioavailability encapsulated therapeutics. Long-term clinical trials are needed validate this approach humans elucidate underlying mechanisms optimal treatment design. If these knowledge gaps addressed, will unavoidably become powerful tool effective along with traditional methods.

Language: Английский

Citations

0
Application of microfluidics for revealing physiological metabolic response of algae at the single-cell level DOI
Yan Qiao,

S LI,

Tingting Liu

et al.

Journal of environmental chemical engineering, Journal Year: 2025, Volume and Issue: unknown, P. 115867 - 115867

Published: Feb. 1, 2025

Language: Английский

Citations

0
Growth performance of Scenedesmus sp. AQUAMEB-57 . Ankistrodesmus sp. AQUAMEB-33, and Synechococcaceae AQUAMEB-32 cultivated at different light intensities DOI
Seyit Uğuz,

Gary A. Anderson,

Ercan Şimşek

et al.

Environmental Technology, Journal Year: 2025, Volume and Issue: unknown, P. 1 - 15

Published: March 11, 2025

Increasing air pollutants significantly contributes to climate change, requiring innovative mitigation strategies. Microalgae provide a promising solution by absorbing CO₂ and like nitrogen oxides (NOx), sulfur (SOx), ammonia from agricultural industrial emissions, while also generating biomass for biofuels animal feed. This study investigated the effects of light intensity on growth biochemical composition Scenedesmus sp. AQUAMEB-57, Ankistrodesmus AQUAMEB-33, Synechococcaceae AQUAMEB-32 cultivated in photobioreactors under two batch continuous culture conditions. reached highest cell concentration (8 × 106 cells ml-1) at 200 µmol photons m-2s-1, peaked 300 m-2s-1. Dry was all species Scendesmus sp showed protein content (15.6%) (17.2%) (23.5%) 100 Maximum carbohydrate sp., 56.0%, 20.5%, 18.4%, respectively, C16/C18 fatty acids increased as raised The findings show that impacts rates profiles, varying cultivation mode. Continuous systems yield higher than systems, emphasizing need optimized strategies enhance algal productivity. research enhances understanding microalgal dynamics, offering insights into optimizing conditions improved supporting sustainable biofuel production other valuable products.

Language: Английский

Citations

0