The role of tumor-associated macrophages in tumor immune evasion

Journal of Cancer Research and Clinical Oncology, Journal Year: 2024, Volume and Issue: 150(5)

Published: May 7, 2024

Abstract Background Tumor growth is closely linked to the activities of various cells in tumor microenvironment (TME), particularly immune cells. During progression, circulating monocytes and macrophages are recruited, altering TME accelerating growth. These adjust their functions response signals from stromal Tumor-associated (TAMs), similar M2 macrophages, key regulators TME. Methods We review origins, characteristics, TAMs within This analysis includes mechanisms through which facilitate evasion promote metastasis. Additionally, we explore potential therapeutic strategies that target TAMs. Results instrumental mediating malignant behaviors. They release cytokines inhibit effector attract additional immunosuppressive primarily T cells, inducing exhaustion directly, influencing activity indirectly cellular interactions, or suppressing checkpoints. directly involved proliferation, angiogenesis, invasion, Summary Developing innovative tumor-targeted therapies immunotherapeutic currently a promising focus oncology. Given pivotal role evasion, several approaches have been devised them. include leveraging epigenetics, metabolic reprogramming, engineering repolarize TAMs, inhibiting recruitment activity, using as drug delivery vehicles. Although some these remain distant clinical application, believe future targeting will offer significant benefits cancer patients.

Language: Английский

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M2 macrophage-derived exosomal miR-26b-5p regulates macrophage polarization and chondrocyte hypertrophy by targeting TLR3 and COL10A1 to alleviate osteoarthritis

Journal of Nanobiotechnology, Journal Year: 2024, Volume and Issue: 22(1)

Published: Feb. 19, 2024

Osteoarthritis (OA) is one of the most prevalent chronic musculoskeletal diseases among elderly population. In this study, macrophage-derived exosomes were isolated and identified. Exosomes subjected to microRNA (miRNA) sequencing bioinformatic analysis, differentially expressed miRNAs verified. miR-26b-5p target genes confirmed through target-site mutation combined with a dual-luciferase reporter assay. The effects on macrophage polarization chondrocyte hypertrophy assessed in vitro. agomir was applied mice OA induced by anterior cruciate ligament transection (ACLT). therapeutic evaluated via pain behavior experiments histological observations. vitro, repolarized M1 macrophages an anti-inflammatory M2 type targeting TLR3 signaling pathway. could COL10A1, further inhibiting macrophage-conditioned medium (M1-CM). vivo, ameliorated gait abnormalities mechanical allodynia mice. treatment attenuated synovitis cartilage degeneration, thereby delaying progression. conclusion, exosomal protect articular ameliorate COL10A1. affected hypertrophy. Thus, miR-26b-5p-based strategy might be potential method for treatment.

Language: Английский

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Hydrogel-exosome system in tissue engineering: A promising therapeutic strategy

Bioactive Materials, Journal Year: 2024, Volume and Issue: 38, P. 1 - 30

Published: April 23, 2024

Characterized by their pivotal roles in cell-to-cell communication, cell proliferation, and immune regulation during tissue repair, exosomes have emerged as a promising avenue for "cell-free therapy" clinical applications. Hydrogels, possessing commendable biocompatibility, degradability, adjustability, physical properties akin to biological tissues, also found extensive utility engineering regenerative repair. The synergistic combination of hydrogels holds the potential not only enhance efficiency but collaboratively advance repair process. This review has summarized advancements made over past decade research hydrogel-exosome systems regenerating various tissues including skin, bone, cartilage, nerves tendons, with focus on methods encapsulating releasing within hydrogels. It critically examined gaps limitations current research, whilst proposed future directions applications this innovative approach.

Language: Английский

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Macrophage-mediated fracture healing: Unraveling molecular mechanisms and therapeutic implications using hydrogel-based interventions

Biomaterials, Journal Year: 2024, Volume and Issue: 305, P. 122461 - 122461

Published: Jan. 1, 2024

Language: Английский

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Bioinspired soft-hard combined system with mild photothermal therapeutic activity promotes diabetic bone defect healing via synergetic effects of immune activation and angiogenesis

Theranostics, Journal Year: 2024, Volume and Issue: 14(10), P. 4014 - 4057

Published: Jan. 1, 2024

Background:The comprehensive management of diabetic bone defects remains a substantial clinical challenge due to the hostile regenerative microenvironment characterized by aggravated inflammation, excessive reactive oxygen species (ROS), bacterial infection, impaired angiogenesis, and unbalanced homeostasis.Thus, an advanced multifunctional therapeutic platform capable simultaneously achieving immune regulation, elimination, tissue regeneration is urgently designed for augmented under pathological milieu.Methods Results: Herein, photoactivated soft-hard combined scaffold system (PGCZ) was engineered introducing polydopamine-modified zeolitic imidazolate framework-8-loaded double-network hydrogel (soft matrix component) into 3D-printed poly(ε-caprolactone) (PCL) (hard component).The versatile PGCZ based on PCL thus prepared features highly extracellular matrix-mimicking microstructure, suitable biodegradability mechanical properties, excellent photothermal performance, allowing long-term structural stability support regeneration.Under periodic near-infrared (NIR) irradiation, localized effect triggers on-demand release Zn 2+ , which, together with repeated mild hyperthermia, collectively accelerates proliferation osteogenic differentiation preosteoblasts potently inhibits growth biofilm formation.Additionally, also presents outstanding immunomodulatory ROS scavenging capacities, which regulate M2 polarization macrophages drive functional cytokine secretion, leading pro-regenerative in situ enhanced vascularization.In vivo experiments further demonstrated that conjunction activity remarkably attenuated local inflammatory cascade, initiated endogenous stem cell recruitment neovascularization, orchestrated osteoblast/osteoclast balance, ultimately accelerating regeneration. Ivyspring

Language: Английский

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Biomaterials for diabetic bone repair: Influencing mechanisms, multi-aspect progress and future prospects

Composites Part B Engineering, Journal Year: 2024, Volume and Issue: 274, P. 111282 - 111282

Published: Feb. 5, 2024

Language: Английский

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M2 microglia-derived exosome-loaded electroconductive hydrogel for enhancing neurological recovery after spinal cord injury

Journal of Nanobiotechnology, Journal Year: 2024, Volume and Issue: 22(1)

Published: Jan. 3, 2024

Abstract Electroconductive hydrogels offer a promising avenue for enhancing the repair efficacy of spinal cord injuries (SCI) by restoring disrupted electrical signals along cord’s conduction pathway. Nonetheless, application composed diverse electroconductive materials has demonstrated limited capacity to mitigate post-SCI inflammatory response. Recent research indicated that transplantation M2 microglia effectively fosters SCI recovery attenuating excessive Exosomes (Exos), small vesicles discharged cells carrying similar biological functions their originating cells, present compelling alternative cellular transplantation. This investigation endeavors exploit microglia-derived exosomes (M2-Exos) successfully isolated and reversibly bonded through hydrogen bonding synergistic promotion synergistically enhance repair. In vitro experiments substantiated significant M2-Exos-laden stimulate growth neural stem axons in dorsal root ganglion modulate microglial polarization. Furthermore, M2-Exos remarkable ability initial reaction within injury site. When combined with hydrogel, worked expedite neuronal axonal regeneration, substantially functional rats afflicted SCI. These findings underscore potential as valuable reparative factor, amplifying foster rehabilitation.

Language: Английский

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Folic acid-modified ginger-derived extracellular vesicles for targeted treatment of rheumatoid arthritis by remodeling immune microenvironment via the PI3K-AKT pathway

Journal of Nanobiotechnology, Journal Year: 2025, Volume and Issue: 23(1)

Published: Jan. 23, 2025

Rheumatoid arthritis (RA), a form of autoimmune inflammation, is marked by enduring synovial inflammation and the subsequent impairment joint function. Despite availability conventional treatments, they are often marred significant side effects associated high costs. Plant-derived extracellular vesicles (PEVs) offer compelling alternative, owing to their abundant availability, affordability, low immunogenicity, biocompatibility, feasibility for large-scale production. These enhance intercellular communication transferring intrinsic bioactive molecules. In our research, we delve into capacity PEVs treat RA, highlighting role ginger-derived (GDEVs). By conjugating GDEVs with folic acid (FA), have developed FA-GDEVs that maintain inherent immunomodulatory properties. designed selectively target M1 macrophages in inflamed joints via folate receptors (FRs). Our vitro findings indicate promote polarization towards reparative M2 macrophage phenotype modulating PI3K-AKT pathway. Further corroboration comes from vivo studies, which demonstrate not only concentrate efficiently affected but also markedly reduce manifestations RA. Synthesizing these findings, it evident emerge as hopeful candidate RA treatment, offering benefits such safety, therapeutic efficacy.

Language: Английский

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Bioactive elements manipulate bone regeneration.

PubMed, Journal Year: 2023, Volume and Issue: 4(4), P. 248 - 269

Published: Jan. 1, 2023

While bone tissue is known for its inherent regenerative abilities, various pathological conditions and trauma can disrupt meticulously regulated processes of formation resorption. Bone engineering aims to replicate the extracellular matrix as well sophisticated biochemical mechanisms crucial effective regeneration. Traditionally, field has relied on external agents like growth factors pharmaceuticals modulate these processes. Although efficacious in certain scenarios, this strategy compromised by limitations such safety issues transient nature compound release half-life. Conversely, bioactive elements zinc (Zn), magnesium (Mg) silicon (Si), have garnered increasing interest their therapeutic benefits, superior stability, reduced biotic risks. Moreover, are often incorporated into biomaterials that function multifaceted components, facilitating regeneration via on-demand. By elucidating mechanistic roles efficacy elements, review establish a robust clinically viable advanced

Language: Английский

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M2 macrophage-derived exosome-functionalized topological scaffolds regulate the foreign body response and the coupling of angio/osteoclasto/osteogenesis

Acta Biomaterialia, Journal Year: 2024, Volume and Issue: 177, P. 91 - 106

Published: Feb. 2, 2024

Language: Английский

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