Additive manufacturing, Journal Year: 2024, Volume and Issue: unknown, P. 104569 - 104569
Published: Nov. 1, 2024
Language: Английский
Journal of Nanobiotechnology, Journal Year: 2024, Volume and Issue: 22(1)
Published: April 20, 2024
Several studies suggest that topographical patterns influence nerve cell fate. Efforts have been made to improve functionality through this approach, focusing on therapeutic strategies enhance function and support structures. However, inadequate orientation can impede long-term efficiency, affecting tissue repair. Therefore, enhancing neurites/axons directional growth is crucial for better outcomes, reducing coiling, ensuring accurate fiber connections. Conflicting results exist regarding the effects of micro- or nano-patterns migration, growth, immunogenic response, angiogenesis, complicating their clinical use. Nevertheless, advances in lithography, electrospinning, casting, molding techniques intentionally control fate neuronal cells are being explored rapidly sustainably efficiency. It appears be accomplished by combining with nanomaterials, biological gradients, electrical stimulation. Despite promising unclear mechanism action, presence cones various directions, restriction outcomes morphological functional markers presented challenges utilizing method. This review seeks clarify how affect morphology function, highlighting potential benefits orientation, especially combined approaches.
Language: Английский
Citations
11
Bioactive Materials, Journal Year: 2024, Volume and Issue: 41, P. 577 - 596
Published: Aug. 24, 2024
Peripheral nervous system (PNS) injuries often lead to significant sensory and motor impairments. Traditional artificial nerve conduits, lacking anisotropic structures, have been associated with prolonged repair time failures in regeneration. This study aimed address these challenges by developing a novel approach for rapid of peripheral (PNI). A 3D oriented fibers scaffold featuring distinct radial (RFs) longitudinal (LFs) orientations was engineered using coaxial electrospinning gas directional foaming techniques. then integrated shape memory conduit form multi-channel micro/nanostructures. The results revealed that the grooved surface significantly improved cellular guidance, effectively facilitating migration SCs from periphery towards center base apex scaffold. In rat model 10 mm defect, ND-PLATMC/LF ND-PCL enhanced regeneration function recovery within 4 weeks. These suggest potential this innovative efficient injuries.
Language: Английский
Citations
11
Materials Today Bio, Journal Year: 2025, Volume and Issue: 31, P. 101503 - 101503
Published: Feb. 6, 2025
Peripheral nerve injuries are a prevalent global issue that has garnered great concern. Although autografts remain the preferred clinical approach to repair, their efficacy is hampered by factors like donor scarcity. The emergence of guidance conduits as novel tissue engineering tools offers promising alternative strategy. This review aims interpret and commercialization from both laboratory perspectives. To enhance comprehension situations, this article provides comprehensive analysis approved United States Food Drug Administration. It proposes initial six months post-transplantation critical window period for evaluating efficacy. Additionally, study conducts systematic discussion on research progress conduits, focusing biomaterials add-on strategies pivotal regeneration, supported literature analysis. conduit materials prospective optimal thoroughly discussed. strategies, together with distinct obstacles potentials deeply analyzed. Based above evaluations, development path manufacturing strategy envisioned. conclusion promoting summarized follows: 1) optimization fundamental means; 2) phased application additional emphasized direction; 3) additive techniques necessary tools. As result, findings provide academic practitioners valuable insights may facilitate future endeavors conduits.
Language: Английский
Citations
1
Advanced Science, Journal Year: 2024, Volume and Issue: 11(31)
Published: June 17, 2024
Abstract Advanced artificial nerve conduits offer a promising alternative for injury repair. Current research focuses on improving the therapeutic effectiveness of by optimizing scaffold materials and functional components. In this study, novel poly(3,4‐ethylenedioxythiophene) (PEDOT)‐integrated fish swim bladder (FSB) is presented as conductive conduit with ordered topology electrical stimulation to promote regeneration. PEDOT nanomaterials adhesive peptides (IKVAV) are successfully incorporated onto decellularized FSB substrate through pre‐coating polydopamine. The obtained PEDOT/IKVAV‐integrated exhibits outstanding mechanical properties, high conductivity, stability, well excellent biocompatibility bioadhesive properties. vitro studies confirm that can effectively facilitate growth directional extension pheochromocytoma 12 cells dorsal root ganglion neurites. addition, in vivo experiments demonstrate proposed accelerate defective repair restoration. findings indicate FSB‐derived multiple regenerative inducing signals integration provide conducive milieu regeneration, exhibiting great potential repairing long‐segment neural defects.
Language: Английский
Citations
7
ACS Applied Bio Materials, Journal Year: 2025, Volume and Issue: unknown
Published: April 1, 2025
Bioactive and biodegradable fibrous conduits consisting of well-organized microfibers with longitudinal grooves on the fiber surface were prepared by electrospinning for nerve guidance conduit (NGC) application. Tubular constructs uniaxially aligned topographical cues have great potential to enhance axonal regeneration are needed bridge large gaps between proximal distal nerves. In this study, we developed electrospun NGCs using milk-derived casein protein (MDP) polycaprolactone polylactic-co-glycolic acid. We designed fabricated a polymer random (RF), (AF), MDP (MDP-RF), (MDP-AF) electrospinning. hypothesized that topographically defined NGC as MDP-AF would outgrowth chemoattraction bioactive peptide in macrophage migration. The vitro results showed not only promotion effect Schwann cell migration polarization but also enhancement PC12 neurite outgrowth. Additionally, demonstrated synergetic effects enhanced injured sciatic To confirm NGC, implanted it into rat (10 mm defect). walking track analysis function, electrophysiological test, gastrocnemius muscle weight, histological immunohistological analyses indicated effectively improved compared other groups at 4 8 weeks. Herein, evolutionally geometric chemotactic stimuli an method combined biocompatible synthetic protein.
Language: Английский
Citations
0
Journal of Materials Science Materials in Medicine, Journal Year: 2025, Volume and Issue: 36(1)
Published: April 9, 2025
Abstract The rapid evolution of three-dimensional printing (3DP) has significantly impacted the medical field. In neurology for instance, 3DP been pivotal in personalized surgical planning and education. Additionally, it facilitated creation implants, microfluidic devices, optogenetic probes, offering substantial implications research applications. 3D printed nasal casts are showing great promise targeted brain drug delivery. also aided creating “phantoms” aligning with advancements neuroimaging, design intricate objects investigating neurobiology sensory perception. Furthermore, emergence bioprinting (3DBP), a fusion cell biology, created new avenues neural tissue engineering. Effective ethical tissue-like biomimetic constructs enabled mechanistic, regenerative, therapeutic evaluations. While individual reviews have explored applications or 3DBP, comprehensive review encompassing success stories across multiple facets both technologies neurosurgery, neuro-regeneration lacking. This aims to consolidate recent achievements 3DBP various neurological science domains encourage interdisciplinary among neurologists, neurobiologists, engineers, order promote further exploration methodologies novel areas practice. Graphical abstract
Language: Английский
Citations
0
Journal of Nanobiotechnology, Journal Year: 2024, Volume and Issue: 22(1)
Published: June 17, 2024
Abstract Background Molybdenum disulfide (MoS 2 ) has excellent physical and chemical properties. Further, chiral MoS (CMS) exhibits chiroptical enantioselective effects, the properties of CMS have been studied for treatment neurodegenerative diseases. Intriguingly, left- right-handed materials different effects on promoting differentiation neural stem cells into neurons. However, effect enantioselectivity peripheral nerve regeneration remains unclear. Methods In this study, CMS@bacterial cellulose (BC) scaffolds were fabricated using a hydrothermal approach. The CMS@BC films synthesized with L-2-amino-3-phenyl-1-propanol was defined as L-CMS. D-2-amino-3-phenyl-1-propanol D-CMS. biocompatibility their Schwann (SCs) validated by cellular experiments. addition, these implanted in rat sciatic defect sites three months. Results These displayed high hydrophilicity, good mechanical properties, low cytotoxicity. we found that L-CMS superior to D-CMS SCs proliferation. After months, showed vivo, conducting velocities 51.2 m/s 26.8 m/s, respectively. better regenerative than scaffolds. Similarly, function index motor electrophysiological functions higher Finally, axon diameter myelin sheath thickness regenerated nerves improved group. Conclusion We can promote regeneration, general, group exhibited repair performance. Overall, findings study reveal be used nanomaterial scaffold repair. Graphical
Language: Английский
Citations
3
Progress in Materials Science, Journal Year: 2025, Volume and Issue: unknown, P. 101497 - 101497
Published: April 1, 2025
Language: Английский
Citations
0
Frontiers in Neuroscience, Journal Year: 2025, Volume and Issue: 19
Published: May 16, 2025
Peripheral nerve injury (PNI) is a challenging clinical problem resulting in disabling sensorimotor deficits, which may become permanent if recovery does not take place timely manner. In this review, we examine recent insights into key molecular mechanisms—particularly MAPK/ERK and PI3K/Akt—that govern Wallerian degeneration, Schwann cell (SC) reprogramming, macrophage polarization. These other critical steps the axonal regeneration process must be understood navigated for therapeutic approach to successful. We highlight emerging strategies, such as electrical stimulation (ES), appears work by activating many of these pro-regenerative gene networks, both neurons non-neuronal support cells. Advances biomaterial engineering, including natural synthetic scaffolds enriched with growth factors, also show promise facilitating across gaps. postulate that integrating optimized ES protocols innovative scaffold designs will allow synergies further enhance functional recovery.
Language: Английский
Citations
0
International Journal of Nanomedicine, Journal Year: 2023, Volume and Issue: Volume 18, P. 6763 - 6779
Published: Nov. 1, 2023
Abstract: Peripheral nerve injuries present significant challenges in regenerative medicine, primarily due to inherent limitations the body’s natural healing processes. In response these and with aim of enhancing peripheral regeneration, nanofiber scaffolds have emerged as a promising advanced intervention. However, deeper understanding underlying mechanistic foundations that drive favorable contributions regeneration is essential. this comprehensive review, we make an exploration latent potential augmenting regeneration. This includes detailed introduction fabrication methods nanofibers, analysis intricate interactions between cellular entities, examination strategies related controlled release bioactive agents, assessment prospects for clinical translation, emerging trends, thorough considerations regarding biocompatibility safety. By comprehensively elucidating structural attributes multifaceted functional capacities scaffolds, offer prospective effective strategy treatment injury. Keywords: nanofiber, nanomedicine, tissue
Language: Английский
Citations
6