Immobilization of KR-12 on a Titanium Alloy Surface Using Linking Arms Improves Antimicrobial Activity and Supports Osteoblast Cytocompatibility DOI Creative Commons
Mohadeseh Zare, Laura Colomina‐Alfaro, Antonella Bandiera

et al.

ACS Applied Bio Materials, Journal Year: 2025, Volume and Issue: unknown

Published: March 28, 2025

Implant-associated infections pose significant challenges due to bacterial resistance antibiotics. Recent research highlights the potential of immobilizing antimicrobial peptides (AMPs) onto implants as an alternative conventional antibiotics for prevention infection. While various AMP immobilization methodologies have been investigated, they lack responsiveness biological cues. This study proposes enzyme-responsive coating orthopedic devices using KR-12, derived from Cathelicidin LL-37, coupled with Human Elastin-Like Polypeptide (HELP) a biomimetic and stimuli-responsive linker, while mimicking extracellular matrix (ECM). During implantation, these customized interfaces encounter innate immune response triggering elastase release, which degrades HELP biopolymers, enabling controlled release KR-12. After coupling KR-12 titanium surfaces, activity against four pathogenic strains (Staphylococcus aureus, Staphylococcus epidermidis, Escherichia coli, Pseudomonas aeruginosa) was assessed, revealing inhibition ratio adhesion colonization exceeding 92% all tested strains, compared surfaces functionalized only. It is thought that enhanced improved mobility when HELP. Furthermore, prepared coatings boosted proliferation human osteoblasts, confirming cytocompatibility. These findings suggest smart combine functions AMPs HELP's properties use in variety settings, including medical devices.

Language: Английский

Developing fibrin-based biomaterials/scaffolds in tissue engineering DOI Creative Commons
Songjie Li,

Xin Dan,

Han Chen

et al.

Bioactive Materials, Journal Year: 2024, Volume and Issue: 40, P. 597 - 623

Published: Aug. 15, 2024

Tissue engineering technology has advanced rapidly in recent years, offering opportunities to construct biologically active tissues or organ substitutes repair even enhance the functions of diseased and organs. Tissue-engineered scaffolds rebuild extracellular microenvironment by mimicking matrix. Fibrin-based possess numerous advantages, including hemostasis, high biocompatibility, good degradability. Fibrin provide an initial matrix that facilitates cell migration, differentiation, proliferation, adhesion, also play a critical role cell-matrix interactions. are now widely recognized as key component tissue engineering, where they can facilitate defect repair. This review introduces properties fibrin, its composition, structure, biology. In addition, modification cross-linking modes fibrin discussed, along with various forms commonly used engineering. We describe biofunctionalization fibrin. provides detailed overview use applications skin, bone, nervous tissues, novel insights into future research directions for clinical treatment.

Language: Английский

Citations

25
Design of bone scaffolds with calcium phosphate and its derivatives by 3D printing: A review DOI

Seyedeh Farnaz Darghiasi,

Ashkan Farazin,

Hanieh Sadat Ghazali

et al.

Journal of the mechanical behavior of biomedical materials/Journal of mechanical behavior of biomedical materials, Journal Year: 2024, Volume and Issue: 151, P. 106391 - 106391

Published: Jan. 9, 2024

Language: Английский

Citations

20
Effects of Silk Fibroin Hydrogel Degradation on the Proliferation and Chondrogenesis of Encapsulated Stem Cells DOI

Tianhao Zhu,

Guolong Cai,

Weikun Zhao

et al.

Biomacromolecules, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 22, 2025

Silk fibroin (SF) hydrogels are widely used in three-dimensional (3D) cell culture and tissue repair. Despite their importance, few studies have focused on regulating degradation further revealing the effects of process encapsulated behaviors. Herein, SF with equivalent initial properties different rates were prepared by adjusting ratios between hydrogel-encapsulated normal microspheres (MSN) enzyme-loaded (MSE). Further, experiments revealed that moderately accelerating hydrogel obviously improved proliferation MSCs during 7 days culture. Slightly promoted MSC chondrogenesis. However, too rapid a was unfavorable for these The relevant expected to provide useful strategies also afford new references development excellent other protein-based biomaterials cartilage regeneration.

Language: Английский

Citations

2
Hybrid Biomechanical Design of Dental Implants: Integrating Solid and Gyroid Triply Periodic Minimal Surface Lattice Architectures for Optimized Stress Distribution DOI Creative Commons
Dawit Bogale Alemayehu, Masahiro TODOH, Song‐Jeng Huang

et al.

Journal of Functional Biomaterials, Journal Year: 2025, Volume and Issue: 16(2), P. 54 - 54

Published: Feb. 9, 2025

Background: Dental implantology has evolved significantly since the introduction of additive manufacturing, which allows for reproduction natural bone’s porous architecture to improve bone tissue compatibility and address stress distribution issues important long-term implant success. Conventional solid dental implants frequently cause shielding, compromises osseointegration reduces durability. Aim: The current research proposes examine biomechanical efficacy fully hybrid gyroid triply periodic minimum surface (TPMS) latticed across different cell sizes optimize Methods: This study evaluates six implants, including designs with three sizes—FLI_111 (1 mm × 1 mm), FLI_222 (2 2 FLI_333 (3 3 mm)—and TPMS necks in corresponding sizes—HI_111, HI_222, HI_333. To enhance initial stability, a square-threaded design was added into bottom part both lattice implants. also incorporate anti-rotational connections fixation, they undergo clinical viability comparison contemporary designs, finite element analysis (FEA) utilized through nTopology (nTOP 4.17.3) balance stiffness flexibility. mechanical performance under realistic conditions, dynamic mastication loading simulation conducted 1.5 s cycles. Results: findings reveal that particularly exhibited improved characteristics by reducing micromotions at bone–implant interface, improving osteointegration, attaining better distribution. Conclusions: By addressing shielding boosting performance, this work paves way personalized developing technology, results.

Language: Английский

Citations

2
Application of Gelatin-Based Composites in Bone Tissue Engineering DOI Creative Commons

Enguang Wu,

Lianghui Huang,

Yaochun Shen

et al.

Heliyon, Journal Year: 2024, Volume and Issue: 10(16), P. e36258 - e36258

Published: Aug. 1, 2024

Natural bone tissue has the certain function of self-regeneration and repair, but it is difficult to repair large damage. Recently, although autologous grafting "gold standard" for improving high cost, few donor sources. Besides, allogeneic causes greater immune reactions, which hardly meet clinical needs. The engineering (BTE) been developed promote repair. Gelatin, due its biocompatibility, receives a great deal attention in BTE research field. However, disadvantages natural gelatin are poor mechanical properties single structural property. With development BTE, often used combination with range natural, synthetic polymers, inorganic materials achieve synergistic effects complex physiological process review delves into fundamental structure unique gelatin, as well excellent necessary scaffold materials. Then this explores application modified three-dimensional (3D) scaffolds various structures including 3D fiber scaffolds, hydrogels, nanoparticles. In addition, focuses on efficacy composite consisting or polymeric materials, bioactive ceramics metallic/non-metallic defects. these gelatin-based provides new ideas design good biosafety.

Language: Английский

Citations

15
Cell-free chitosan/silk fibroin/bioactive glass scaffolds with radial pore for in situ inductive regeneration of critical-size bone defects DOI
Xinsong Zhang, Yijing Xia,

Jie Xu

et al.

Carbohydrate Polymers, Journal Year: 2024, Volume and Issue: 332, P. 121945 - 121945

Published: Feb. 13, 2024

Language: Английский

Citations

14
The role of graphene quantum dots in cutting‐edge medical therapies DOI

Kosar Arab,

Aliakbar Jafari,

Farangis Shahi

et al.

Polymers for Advanced Technologies, Journal Year: 2024, Volume and Issue: 35(9)

Published: Sept. 1, 2024

Abstract Graphene quantum dots (GQDs), owing to their unique optical, electrical, and chemical properties, have emerged as promising nanomaterials for various biomedical applications. This review provides a comprehensive overview of the latest advancements in utilization GQDs tissue engineering, wound healing, drug delivery systems, other therapies. The inherent properties GQDs, including high biocompatibility, tunable photoluminescence, significant surface area, make them ideal candidates enhancing medical treatments diagnostics. In improve mechanical biological performance scaffolds, promoting cell proliferation differentiation. For enhance antimicrobial activity facilitate faster regeneration. Their potential DDS is highlighted by ability deliver therapeutic agents efficiently, ensuring targeted controlled release. Additionally, play crucial role therapies, particularly cancer treatment, efficacy reducing side effects. While offer diagnostics, challenges such understanding long‐term cytotoxicity at higher concentrations, need standardized synthesis methods remain critical areas further research. also discusses future directions opportunities emphasizing transformative advancing modern healthcare solutions. insights presented here contribute expanding field GQD research, highlighting significantly patient outcomes drive innovations.

Language: Английский

Citations

11
Hierarchical Mineralized Collagen Coated Zn Membrane to Tailor Cell Microenvironment for Guided Bone Regeneration DOI Open Access

Fanyu Yan,

Min Yu, Yunjiao He

et al.

Advanced Functional Materials, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 10, 2025

Abstract Maxillofacial bone defects caused by trauma, infections, tumors, and congenital disorders have become a significant global health concern. An ideal guided regeneration (GBR) membrane is expected to not only act as barrier but also promote osteoinduction osteogenesis. However, non‐resorbable membranes limited bioactivity require secondary surgeries, whereas resorbable often lack the required mechanical properties degrade too quickly. To address these challenges, bilayer self‐induced GBR constructed combining pure zinc (Zn) hierarchical mineralized collagen (HMC) via self‐assembly, termed ss‐HMC/Zn. The Zn layer acts an outer toward soft tissue that provides spatiotemporal support, HMC creates favorable internal osteogenic microenvironment. Moreover, bone‐like nanostructure of effectively controlled release ions. In vitro assays demonstrates adequate maintenance, excellent cytocompatibility, ability membrane. vivo results show superior osteoinductive capability without need for supplements. Furthermore, ss‐HMC/Zn exhibits immunomodulatory functions, promoting angiogenesis cell recruitment, thereby shedding light on underlying mechanisms. conclusion, well‐orchestrated provide new insights into maxillofacial defects.

Language: Английский

Citations

1
A metamaterial scaffold beyond modulus limits: enhanced osteogenesis and angiogenesis of critical bone defects DOI Creative Commons
Yu Qin, Zehao Jing, Da Zou

et al.

Nature Communications, Journal Year: 2025, Volume and Issue: 16(1)

Published: March 4, 2025

Metallic scaffolds have shown promise in regenerating critical bone defects. However, limitations persist achieving a modulus below 100 MPa due to insufficient strength. Consequently, the osteogenic impact of lower and greater tissue strain ( > 1%) remains unclear. Here, we introduce metamaterial scaffold that decouples strength through two-stage deformation. The facilitates an effective only 13 MPa, ensuring adaptability during regeneration. Followed by stiff stage, it provides necessary for load-bearing requirements. In vivo, induces 2% callus strain, upregulating calcium channels HIF-1α enhance osteogenesis angiogenesis. 4-week histomorphology reveals 44% 498% increase new fraction versus classic with 500 modulus, respectively. This design transcends traditional modulus-matching paradigms, prioritizing Its tunable mechanical properties also present promising implications advancing mechanisms addressing clinical challenges.

Language: Английский

Citations

1
Computational Modelling and Simulation of Scaffolds for Bone Tissue Engineering DOI Creative Commons
Haja-Sherief N Musthafa, Jason Walker, Mariusz Domagała

et al.

Computation, Journal Year: 2024, Volume and Issue: 12(4), P. 74 - 74

Published: April 4, 2024

Three-dimensional porous scaffolds are substitutes for traditional bone grafts in tissue engineering (BTE) applications to restore and treat injuries defects. The use of computational modelling is gaining momentum predict the parameters involved healing cell seeding procedures perfusion bioreactors reach final goal optimal growth. Computational based on finite element method (FEM) fluid dynamics (CFD) two standard methodologies utilised investigate equivalent mechanical properties scaffolds, as well flow characteristics inside respectively. success a simulation hinges selection relevant mathematical model with proper initial boundary conditions. This review paper aims provide insights researchers regarding appropriate (FE) models different materials CFD regimes bioreactors. Thus, these FEM/CFD may help create efficient designs by predicting their structural haemodynamic responses prior vitro vivo (TE) applications.

Language: Английский

Citations

7