Springer eBooks, Journal Year: 2024, Volume and Issue: unknown, P. 85 - 126
Published: Jan. 1, 2024
Language: Английский
Applied Sciences, Journal Year: 2024, Volume and Issue: 14(17), P. 7912 - 7912
Published: Sept. 5, 2024
Recent decades clearly demonstrate the growing use of nanomaterials in medical practice, and their effectiveness is systematically confirmed by consequent scientific research. An example dentistry endodontic treatment, which, due to its specificity, one most demanding procedures, fraught with numerous challenges, such as difficulties reaching tooth roots ineffective cleaning or insufficient sealing root canals, which may lead re-infection damage adjacent structures. The has a positive impact on solving these problems, combination biomaterials nanometric technology makes treatment more effective, precise comfortable for patients, contributes improving quality dental care. Currently, high biocompatibility can be used endodontics components irrigation solutions, rinsing canals drug carriers intracanal use. Nanomaterials are also sealants filling canals. However, latest research shows that reducing size materials “nano” scale significantly affects basic physicochemical properties, leads increased reactivity ability interact at molecular level. These unique have contributed medical-related raise concerns provoke discussions about safety direct contact tissues.
Language: Английский
Citations
6
Cells, Journal Year: 2025, Volume and Issue: 14(3), P. 201 - 201
Published: Jan. 29, 2025
Cell homing, a process that leverages the body’s natural ability to recruit cells and repair damaged tissues, presents promising alternative cell transplantation methods. Central this approach is recruitment of endogenous stem/progenitor cells—such as those from apical papilla, bone marrow, periapical tissues—facilitated by chemotactic biological cues. Moreover, biomaterial scaffolds embedded with signaling molecules create supportive environments, promoting migration, adhesion, differentiation for regeneration pulp–dentin complex. By analyzing in vivo animal studies using homing strategies, review explores how biomolecules scaffold materials enhance stem site dental pulp tissue, thereby regeneration. It also examines key principles, recent advancements, current limitations linked homing-based regenerative endodontic therapy, highlighting interplay biomaterials, molecules, their broader clinical implications.
Language: Английский
Citations
0
European Archives of Paediatric Dentistry, Journal Year: 2025, Volume and Issue: unknown
Published: Jan. 31, 2025
The purpose of this study is to evaluate the influence photobiomodulation therapy on regenerative potential non-vital mature permanent teeth healthy Canine dogs. 54 roots, obtained from 27 premolars, in dogs, were selected and divided into three equal groups where Group I received endodontic procedures (REPs) using blood clot as a scaffold (positive control), II similar treatment I, followed by (study group) III did not receive any intervention (negative control). Each group was further subgroups for evaluation vascular area percentage fibroblast count at 3 different intervals; 3, 10, 15 weeks. Statistical analysis performed with R statistical software version 4.1.3 RESULTS: Data percentages, revealed an insignificant increase mean values 10 weeks both positive control group, all significantly lower than negative (p < 0.001). At weeks, there difference between 9.76 ± 2.25, 11.97 2.37, respectively. However, higher (2.47 1.0), Regarding count, gradual recorded intervals, reaching its maximum 15th week period; they 41.40 1.14, 56.60 6.11 44.67 17.04 control, respectively, differences control. results support revascularization alternative modality. Photobiomodulation could improve construction regenerated vessels fibroblasts. studies longer flow-up periods animal models are recommended.
Language: Английский
Citations
0
Frontiers in Cell and Developmental Biology, Journal Year: 2025, Volume and Issue: 13
Published: Feb. 19, 2025
Peripheral nerve injuries, resulting from trauma or medical interventions, present significant clinical challenges due to their severe physiological and functional impacts. Despite various therapeutic approaches, optimal methods for promoting regeneration remain difficult obtain. This study is a preliminary step towards the future use of chitosan guide conduits combined with human dental pulp stem cells conditioned media promote regrowth in rat model sciatic damage. Preliminary characterization medium identified key regenerative biomarkers using Multiplexing LASER Bead analysis. The cells' cytocompatibility Reaxon® biomaterial was confirmed through viability metabolic assays PrestoBlue assay, along scanning electron microscopy energy-dispersive X-ray spectroscopy analyses. These vitro assessments validated potential combinations regeneration. Future vivo experiments will involve applying these model, evaluate efficacy. research aims establish as viable treatments peripheral injury, offering promising directions applications.
Language: Английский
Citations
0
Dentistry, Journal Year: 2025, Volume and Issue: unknown
Published: Feb. 13, 2025
Traumatic dental injuries (TDIs) represent a significant global healthcare challenge, affecting different age groups and socioeconomic strata. This comprehensive chapter presents contemporary advances in diagnosing, managing, preventing TDIs, integrating evidence-based approaches with emerging technologies biological interventions. The text systematically addresses the multifaceted nature of trauma, from immediate emergency response to long-term rehabilitation, while considering broader implications for systems society. Recent developments diagnostic technologies, including artificial intelligence-assisted imaging advanced assessment tools, have transformed initial evaluation trauma. explores innovative treatment modalities, encompassing regenerative endodontics, stem cell applications, computer-guided interventions alongside traditional approaches. Particular emphasis is placed on digital workflows, teledentistry minimally invasive techniques that revolutionized trauma management. critically examines impact TDI, direct indirect costs, quality life considerations, resource allocation. Special attention given age-specific management protocols, medically compromised patients, legal-ethical considerations. concludes an analysis future directions traumatology, providing framework continued advancement.
Language: Английский
Citations
0
Frontiers in Bioengineering and Biotechnology, Journal Year: 2025, Volume and Issue: 13
Published: April 24, 2025
Introduction Angiogenesis represents a critical challenge in dental pulp regeneration due to the tissue’s restricted nutrient supply through 0.5-mm apical foramen. While stem cells (DPSCs) hold regenerative potential, their limited vascularization capacity impedes clinical applications. Through Single-cell RNA sequencing (scRNA-seq) analysis of human pulp, we discovered PDGF (+) mesenchymal subset exhibiting enhanced angiogenic signatures, suggesting targeted cell selection could overcome this bottleneck. Methods ScRNA-seq identified subpopulation samples, validated multiplex immunohistochemical localization PDGF/CD73/CD31. PDGF-BB-overexpressing DPSCs were engineered via lentiviral vectors. Functional assessments included: 1) CCK-8/Edu/cell cycle/transwell assays for proliferation and migration ability 2) HUVECs co-culture models analyzing chemotaxis tube formation 3) Vascularized tissue rat kidney capsule transplants. Results Discussion The CD73 demonstrated spatial correlation with CD31 vasculature. PDGF-BB overexpression DPSCs' proliferative capacity. Co-cultured exhibited increased group. In vivo transplants generated more vascular structures containing endothelia. These findings establish engineering as an effective strategy amplify while emphasizing therapeutic value functionally-defined subpopulations regeneration.
Language: Английский
Citations
0
BMC Oral Health, Journal Year: 2024, Volume and Issue: 24(1)
Published: Aug. 22, 2024
This study aimed to compare the in vitro shear bond strength (SBS) of mineral trioxide aggregate (MTA) and dual-cured, resin-modified calcium silicate material (Theracal PT) composite resin, compomer, bulk-fill composite, evaluate failure mode under a stereomicroscope. Ninety acrylic specimens, each with 4 mm diameter 2 height central hole, were prepared. These specimens randomly divided into two groups based on capping materials: MTA Theracal PT. Each group was further subdivided three subgroups (n = 15) according restorative composite. The then subjected testing using universal machine at crosshead speed 1 mm/min. Post-test, fracture locations examined Data analyzed two-way analysis variance (ANOVA) Tukey test. SBS values for PT significantly higher than those (p < 0.001). Within groups, no significant differences observed across different materials. However, difference found between mean + resin compomer shows promise dentistry due its superior strength. Given values, appears capable forming durable long-lasting restorations by establishing reliable bonds various materials commonly used dentistry.
Language: Английский
Citations
1
Odontology, Journal Year: 2024, Volume and Issue: unknown
Published: July 5, 2024
Language: Английский
Citations
0
Springer eBooks, Journal Year: 2024, Volume and Issue: unknown, P. 73 - 83
Published: Jan. 1, 2024
Language: Английский
Citations
0
Springer eBooks, Journal Year: 2024, Volume and Issue: unknown, P. 85 - 126
Published: Jan. 1, 2024
Language: Английский
Citations
0