The integration of environmental governance and 3D printing technology: Current situation and prospects

Chemical Engineering Journal, Journal Year: 2025, Volume and Issue: unknown, P. 159498 - 159498

Published: Jan. 1, 2025

Language: Английский

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Advancing Precision Surgery: The Role of 3D Printing in Liver Surgery

3D Printing and Additive Manufacturing, Journal Year: 2025, Volume and Issue: unknown

Published: March 7, 2025

Language: Английский

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Bioprinted high cell density liver model with improved hepatic metabolic functions

Biomaterials, Journal Year: 2025, Volume and Issue: 320, P. 123256 - 123256

Published: March 12, 2025

Language: Английский

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Cell therapy for liver disorders: past, present and future

Nature Reviews Gastroenterology & Hepatology, Journal Year: 2025, Volume and Issue: unknown

Published: March 18, 2025

Language: Английский

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Liver cancer: Current status of preclinical research

Elsevier eBooks, Journal Year: 2025, Volume and Issue: unknown, P. 1 - 34

Published: Jan. 1, 2025

Language: Английский

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3D bioprinting for bile duct tissue engineering: current status and prospects

Frontiers in Bioengineering and Biotechnology, Journal Year: 2025, Volume and Issue: 13

Published: April 14, 2025

Bile duct disorders, including cholangiocarcinoma, primary sclerosing cholangitis, and iatrogenic injuries, pose significant clinical challenges due to limited regenerative capacity the complexity of biliary tree. In recent years, 3D bioprinting has emerged as a promising approach for bile tissue engineering by providing patient-specific geometries facilitating spatial organization cells, scaffolding materials, bioactive factors. This review presents comprehensive overview techniques engineering, focusing on fundamental principles, biomaterial selection, current achievements, key challenges, future perspectives. We systematically discuss latest technological breakthroughs, highlight emerging innovations such organoid-based strategies microfluidic-assisted printing, evaluate prospects translation. Finally, we outline main challenges—such biocompatibility vascularization, immunological barriers, standardization protocols, regulatory hurdles—and propose directions research, emphasizing multidisciplinary collaboration translational studies.

Language: Английский

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Alternatives to animal testing to assess MASH drugs and hepatotoxicity

Hepatology, Journal Year: 2023, Volume and Issue: unknown

Published: Nov. 6, 2023

The Food and Drug Administration (FDA) Modernization Act 2.0 "allows for alternatives to animal testing purposes of drug biological product applications." This provides an opportunity develop improve studies assess drugs in the liver. Two-dimensional cultures liver cells fail maintain their differentiated state reproduce disease phenotypes. Therefore, several platforms using human are being developed either (1) hepatotoxicity or (2) create "diseases a dish" effectiveness treating diseases, primarily focused on MASH. technological approaches include precision cut slices, spheroids, organoids, bioprinted tissues, microphysiological systems. review evaluates each these technologies role providing animals.

Language: Английский

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Microscale tissue engineering of liver lobule models: advancements and applications

Frontiers in Bioengineering and Biotechnology, Journal Year: 2023, Volume and Issue: 11

Published: Dec. 8, 2023

The liver, as the body's primary organ for maintaining internal balance, is composed of numerous hexagonal liver lobules, each sharing a uniform architectural framework. These lobules serve basic structural and functional units comprised central veins, hepatic plates, sinusoids, minute bile ducts. Meanwhile, within distinct regions hepatocytes carry out diverse functions. in vitro construction lobule models, faithfully replicating their structure function, holds paramount significance research development diseases. Presently, two technologies constructing models dominate field: 3D bioprinting microfluidic techniques. enables precise deposition cells biomaterials, while microfluidics facilitates targeted transport or other culture materials to specified locations, effectively managing media input output through micro-pump control, enabling dynamic simulations lobules. In this comprehensive review, we provide an overview cells, manufacturing methods employed by recent researchers models. Our aim explore strategies that closely emulate authentic function offering invaluable insights into diseases, drug screening, toxicity assessment, cell replacement therapy.

Language: Английский

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Contribution of the ELRs to the development of advanced in vitro models

Frontiers in Bioengineering and Biotechnology, Journal Year: 2024, Volume and Issue: 12

Published: April 8, 2024

Developing in vitro models that accurately mimic the microenvironment of biological structures or processes holds substantial promise for gaining insights into specific functions. In field tissue engineering and regenerative medicine, able to capture precise structural, topographical, functional complexity living tissues, prove be valuable tools comprehending disease mechanisms, assessing drug responses, serving as alternatives complements animal testing. The choice right biomaterial fabrication technique development these plays an important role their functionality. this sense, elastin-like recombinamers (ELRs) have emerged tool overcoming challenges encountered natural synthetic materials due intrinsic properties, such phase transition behavior, tunable viscoelasticity, easy processability. review article, we will delve use ELRs molecular intrinsically disordered proteins (IDPs), well 3D medicine. processability rational design has allowed spheroids organoids, bioinks bioprinting. Thus, incorporating toolkit biomaterials used models, represents a transformative step forward improving accuracy, efficiency, functionality opening up wide range possibilities combination with advanced biofabrication techniques remains explored.

Language: Английский

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Induction of MASH in three-dimensional bioprinted human liver tissue

PLoS ONE, Journal Year: 2024, Volume and Issue: 19(12), P. e0312615 - e0312615

Published: Dec. 30, 2024

Metabolic dysfunction-associated steatohepatitis (MASH), formerly known as nonalcoholic is a major risk factor for cirrhosis and hepatocellular carcinoma (HCC) leading cause of liver transplantation. MASH caused by an accumulation toxic fat molecules in the hepatocyte which leads to inflammation fibrosis. Inadequate human “MASH dish” models have limited our advances understanding pathogenesis drug discovery. This study uses complex multicellular 3D bioprinting, combining hepatocytes with nonparenchymal cells physiologically relevant cell ratios using biocompatible hydrogels generate bioinks Bioprinted tissues consisting four types, (hepatocytes, endothelial cells, Kupffer hepatic stellate cells) are generated from purified normal livers, this bioprinting platform. These incubated cocktail fatty acids, lipopolysaccharide (LPS), fructose produce phenotype comparison control media. Furthermore, these bioprinted sufficient size undergo histological processing immunohistchemistry comparable classic clinical pathological analysis. The develop steatosis, inflammation, fibrosis, response induction Additionally, transcriptome differed significantly healthy more closely resembled biopsies livers patients Thus, has developed tissue suitable studies on pathophysiology

Language: Английский

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