Unique Role of Vimentin Networks in Compression Stiffening of Cells and Protection of Nuclei from Compressive Stress

Nano Letters, Journal Year: 2022, Volume and Issue: 22(12), P. 4725 - 4732

Published: June 9, 2022

In this work, we investigate whether stiffening in compression is a feature of single cells and the intracellular polymer networks that comprise cytoskeleton (all which stiffen with increasing shear strain) or soften when subjected to compressive strains. We find individual cells, such as fibroblasts, at physiologically relevant strains, but genetic ablation vimentin diminishes effect. Further, show unlike purified F-actin microtubules, compression, intermediate filament both extension, present theoretical model explain response based on flexibility filaments their surface charge, resists volume changes network under compression. These results provide new framework by understand mechanical responses point central role

Language: Английский

---

Innate and Adaptive Immunity during SARS-CoV-2 Infection: Biomolecular Cellular Markers and Mechanisms

Vaccines, Journal Year: 2023, Volume and Issue: 11(2), P. 408 - 408

Published: Feb. 10, 2023

The coronavirus 2019 (COVID-19) pandemic was caused by a positive sense single-stranded RNA (ssRNA) severe acute respiratory syndrome 2 (SARS-CoV-2). However, other human coronaviruses (hCoVs) exist. Historical pandemics include smallpox and influenza, with efficacious therapeutics utilized to reduce overall disease burden through effectively targeting competent host immune system response. is composed of primary/secondary lymphoid structures initially eight types cell types, many subtypes, traversing membranes utilizing signaling cascades that contribute towards clearance pathogenic proteins. Other proteins discussed cluster differentiation (CD) markers, major histocompatibility complexes (MHC), pleiotropic interleukins (IL), chemokines (CXC). historical concepts immunity are the innate adaptive systems. represented T cells, B antibodies. macrophages, neutrophils, dendritic complement system. viruses can affect regulate cycle progression for example, in cancers papillomavirus (HPV: cervical carcinoma), Epstein-Barr virus (EBV: lymphoma), Hepatitis C (HB/HC: hepatocellular carcinoma) Leukemia Virus-1 (T leukemia). Bacterial infections also increase risk developing cancer (e.g.,

Language: Английский

---

Exploring new frontiers: cell surface vimentin as an emerging marker for circulating tumor cells and a promising therapeutic target in advanced gastric Cancer

Journal of Experimental & Clinical Cancer Research, Journal Year: 2024, Volume and Issue: 43(1)

Published: April 30, 2024

Abstract Background Circulating tumor cells (CTCs) hold immense promise in guiding treatment strategies for advanced gastric cancer (GC). However, their clinical impact has been limited due to challenges identifying epithelial-mesenchymal transition (EMT)-CTCs using conventional methods. Methods To bridge this knowledge gap, we established a detection platform CTCs based on the distinctive biomarker cell surface vimentin (CSV). A prospective study involving 127 GC patients was conducted, comparing enumeration both EpCAM and CSV. This approach enabled of regular EMT-CTCs, providing comprehensive analysis. Spiking assays WES were employed verify reliability marker technique. explore potential inducer CSV + formation, combination Tandem Mass Tag (TMT) quantitative proteomics, m 6 RNA immunoprecipitation–qPCR (MeRIP–qPCR), single-base elongation- ligation-based qPCR amplification method (SELECT) sequencing (RNA-seq) utilized screen confirm target gene. Both vitro vivo experiments performed molecular mechanism expression regulation its role metastasis. Results Our findings revealed predicting therapeutic responses long-term prognosis patients. Additionally, compared EpCAM-based method, CSV-specific positive selection assay significantly better evaluating response successfully predicted disease progression 14.25 months earlier than radiology evaluation. Apart from excellent as marker, emerges promising attenuating It found that fat mass obesity associated protein (FTO) could act catalyst insulin-like growth factor-I receptor (IGF-IR) mRNA decay through modification. The activation IGF-I/IGF-IR signaling enhanced translocation cytoplasm phosphorylation at serine 39 (S39). In mouse model, simultaneous inhibition blockade IGF-IR pathway yielded outcomes. Conclusion summary, leveraging universal represents significant breakthrough advancing personalized medicine with GC. research not only paves way tailored but also underscores pivotal enhancing management, opening new frontiers precision medicine.

Language: Английский

---

METTL1 facilitates ameloblastoma invasive growth via MAPK signaling pathway

Gene, Journal Year: 2024, Volume and Issue: 905, P. 148234 - 148234

Published: Feb. 2, 2024

Ameloblastoma (AM), a common odontogenic epithelial tumor, exhibits aggressive growth due to incomplete encapsulation within the jawbone. Postoperative recurrence is significant concern, closely associated with its invasive nature. We investigate role of tRNA N-7 methylguanosine (m

Language: Английский

---

Vimentin undergoes liquid–liquid phase separation to form droplets which wet and stabilize actin fibers

Proceedings of the National Academy of Sciences, Journal Year: 2025, Volume and Issue: 122(10)

Published: March 3, 2025

The cytoskeleton is composed of F-actin, microtubules, and intermediate filaments (IFs). Vimentin one the most ubiquitous well-studied IFs. It involved in many activities including wound healing, tissue fibrosis, cancer metastasis, all which require rapid vimentin IF assembly. In this paper, we report that forms liquid condensates appear to enable filament growth. Given transient nature these droplets, focus on properties vimentin-Y117L, has a point mutation leads formation but not IFs, enabling us study droplets detail. dissolve under 1,6-Hexanediol treatment decreasing concentration, confirming they are liquid, phase separated. These extensively wet actin stress fibers, rendering them resistant actin-binding drugs protecting from depolymerization. We show similar behavior occurs wild-type during its assembly into filaments.

Language: Английский

---

Signaling Pathways and Protein–Protein Interaction of Vimentin in Invasive and Migration Cells: A Review

Cellular Reprogramming, Journal Year: 2022, Volume and Issue: 24(4), P. 165 - 174

Published: June 24, 2022

The vimentin (encoded by VIM) is one of the 70 human intermediate filaments (IFs), building highly dynamic and cell-type-specific web networks in cytoplasm. Vim−/− mice exhibit process defects associated with cell differentiation, which can have implications for understanding cancer disease. This review showed recent reports from studies that unveiled (VIFs) as an essential component cytoskeleton, followed a description vimentin's physiological functions VIF signaling pathway gene network studies. main focus discussion on vital pathways how coordinates invasion cells migration. current research will open up multiple processes to function other IF proteins cellular molecular biology, they lead insights into different levels invasive metastatic cells. Enrich GO databases used Gene Ontology Pathway Enrichment Analysis. Estimation STRING online was predict functional interactions proteins–protein Cytoscape analysis search select master genes. Using analysis, we presented eight genes, RhoA, Smad3, Akt1, Cdk2, Rock1, Rock2, Mapk1, Mapk8, protein–protein interaction involved invasion.

Language: Английский

---

Proactive and reactive roles of TGF-β in cancer

Seminars in Cancer Biology, Journal Year: 2023, Volume and Issue: 95, P. 120 - 139

Published: Aug. 10, 2023

Cancer cells adapt to varying stress conditions survive through plasticity. Stem exhibit a high degree of plasticity, allowing them generate more stem or differentiate into specialized cell types contribute tissue development, growth, and repair. can also plasticity acquire properties that enhance their survival. TGF-β is an unrivaled growth factor exploited by cancer gain TGF-β-mediated signaling enables carcinoma alter epithelial mesenchymal epithelial-mesenchymal (EMP). However, multifunctional cytokine; thus, the be detrimental beneficial depending on cellular context. Those overcome anti-tumor effect induce transition (EMT) EMP benefits. allows tumor immune microenvironment (TIME), facilitating Due significant roles in progression, it essential understand how exploit this This understanding will guide development effective TGF-β-targeting therapies eliminate

Language: Английский

---

Placental Vimentin Expression in Preeclampsia and Gestational Diabetes Mellitus

Gynecology Obstetrics and Reproductive Medicine, Journal Year: 2024, Volume and Issue: unknown, P. 1 - 9

Published: April 8, 2024

OBJECTIVE: This study investigated vimentin expression in placentas of patients with preeclampsia and gestational diabetes mellitus (GDM). STUDY DESIGN: Placentas preeclamptic women (n=25), GDM control cases (n=25) were enrolled this study. Placental samples fixed zinc-formalin further processed for paraffin wax tissue embedding. Demographic laboratory parameters recorded. Vimentin immune activity was analyzed the placental sections immunohistochemistry. Sections imaged under a light microscope. A semiquantitative measurement done between groups by comparing signal significance calculated. Network construction pathway enrichment analysis conducted using Cytoscape (v3.10.1) ShinyGO, respectively. RESULTS: high group. The group showed positive cytotrophoblast syncytiotrophoblast cells connective villi generally recorded as negative villi, fibrinoid substances, Bioinformatic that AGE-RAGE signaling cancer-related pathways mainly observed Vimentin-associated pathways, which finally activate inflammatory both GDM. CONCLUSION: patterns reveal nuanced regulatory mechanisms, emphasizing need exploration into functional roles physiology pathology.

Language: Английский

---

A Systematic Review of Proteomics in Obesity: Unpacking the Molecular Puzzle

Current Obesity Reports, Journal Year: 2024, Volume and Issue: 13(3), P. 403 - 438

Published: May 4, 2024

The present study aims to review the existing literature identify pathophysiological proteins in obesity by conducting a systematic of proteomics studies. Proteomics may reveal mechanisms development and clarify links between related diseases, improving our comprehension its clinical implications.

Language: Английский

---

The Role of RKIP in the Regulation of EMT in the Tumor Microenvironment

Cancers, Journal Year: 2022, Volume and Issue: 14(19), P. 4596 - 4596

Published: Sept. 22, 2022

The Raf Kinase Inhibitor Protein (RKIP) is a unique gene product that directly inhibits the Raf/Mek/Erk and NF-kB pathways in cancer cells resulting inhibition of cell proliferation, viability, EMT, metastasis. Additionally, RKIP involved regulation resistance to both chemotherapy immunotherapy. low expression many types responsible, part, for pathogenesis its multiple properties. EMT metastasis by led classification as tumor suppressor. However, mechanism which mediates inhibitory effects on metastases was not clear. We have proposed one involves negative various products mediate mesenchymal phenotype well positive epithelial via signaling cross talks between each product. examined several such Snail, vimentin, N-cadherin, laminin EPCAM E-cadherin laminin. found indeed these correlations were detected cross-talks. In addition, we also bioinformatic data sets different human cancers findings corroborated, large observed cross-talks with few exceptions some types. overall support underlying suppressor regulates Hence, development agent can selectively induce expressions should result activation pleiotropic anti-cancer activities including sensitization resistant respond chemotherapeutics immunotherapeutics.

Language: Английский

---