Enhanced control efficacy of Bacillus subtilis NM4 via integration of chlorothalonil on potato early blight caused by Alternaria solani

Microbial Pathogenesis, Journal Year: 2024, Volume and Issue: 190, P. 106604 - 106604

Published: March 14, 2024

Language: Английский

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Bacillus velezensis BVE7 as a promising agent for biocontrol of soybean root rot caused by Fusarium oxysporum

Frontiers in Microbiology, Journal Year: 2023, Volume and Issue: 14

Published: Oct. 20, 2023

Soybean root rot (SRR), caused by Fusarium oxysporum, is a severe soil-borne disease in soybean production worldwide, which adversely impacts the yield and quality of soybean. The most effective method for managing crop diseases decreasing reliance on chemical fungicides, such as Bacillus spp., via microbial biocontrol agents.In this study, soil-isolated strain BVE7 was identified B. velezensis, exhibiting broad-spectrum activity against various pathogens causing rot. sterile filtrate, at concentration 10%, demonstrated significant antifungal inhibiting conidial germination, production, mycelial growth F. oxysporum 61.11%, 73.44%, 85.42%, respectively, hyphal malformations. compound produced adaptability to standard environment. pot experiment showed that suspension could effectively control rot, with highest efficiency 75.13%. Furthermore, it considerably enhanced catalase, phenylalanine ammonia lyase, superoxide dismutase, peroxidase roots, while also preventing an increase malondialdehyde activity. By improving host resistance towards pathogens, damage fungi severity have been reduced.This study presents innovative utilization isolated from roots cold conditions, controlling oxysporum. findings highlight remarkable regional adaptive characteristics strain, making excellent candidate combating diverse environments. In conclusion, velezensis potential reducing SRR incidence can be considered viable option management.

Language: Английский

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Role of an antagonistic bacterium, Bacillus subtilis PE7, in growth promotion of netted melon (Cucumis melo L. var. reticulatus Naud.)

Canadian Journal of Microbiology, Journal Year: 2023, Volume and Issue: 70(2), P. 40 - 51

Published: Nov. 2, 2023

The aim of this study was to determine the plant growth-promoting effect Bacillus subtilis PE7 on growth melon plants. B. isolated from kimchi identified based colonial and microscopic morphology along with analyses 16S rRNA pycA gene sequences. Strain showed different levels inhibition phytopathogens able grow at variable temperatures pH values. had ability produce siderophores, indole-3-acetic acid (IAA), ammonia, exopolysaccharides, 1-aminocyclopropane-1-carboxylic deaminase, as well solubilize insoluble phosphate zinc. IAA secretion strain a concentration-dependent pattern concentration l-tryptophan supplemented in fertilizer-based culture medium. LC-MS analysis indicates presence filtrate PE7. Treatment containing metabolites, mainly IAA, significantly promoted terms higher parameters greater nutrient contents compared treatments without fertilizer, water. cells attached firmly colonized roots bacterized Based our results, can be utilized potential microbial fertilizer substitute chemical fertilizers sustainable agriculture.

Language: Английский

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Exploring mechanisms of compost-mediated suppression of plant pathogens: A critical review

Applied Soil Ecology, Journal Year: 2024, Volume and Issue: 203, P. 105644 - 105644

Published: Sept. 16, 2024

Language: Английский

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Comparative Effects of Potassium Iodate and SAAF-Fungicide on the Control of Fusarium Wilt (Fusarium oxysporum) And the Yield of Pepper (Capsicum spp. L.)

Journal of applied science and environmental management, Journal Year: 2024, Volume and Issue: 28(2), P. 415 - 418

Published: Feb. 25, 2024

SAAF Fungicide is a systemic and contact fungicide that used to prevent leaf spot, blast disease, rust other types of fungi pests in all kinds crops, especially on vegetable plant. Hence, the objective this paper as compare effects Potassium Iodate SAAF-fungicide control Fusarium Wilt (Fusarium oxysporum) Yield Pepper (Capsicum spp L.) using appropriate standard methods. Agronomic data such number leaves, plant height flowers was collected. Disease incidence severity well yield were measured analyzed Analysis Variance (ANOVA). Among treatment, Gulpinar F1 with KIO3 has highest leaves across experiment while negative least leaves. De cayenne pepper harvested followed by KIO3, disease infection severity, there are very mild treated plots. The two treatments have positive effect growth, pepper. achieved from sample.

Language: Английский

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Butyl succinate-mediated control of Bacillus velezensis ce 100 for apple anthracnose caused by Colletotrichum gloeosporioides

Journal of Applied Microbiology, Journal Year: 2023, Volume and Issue: 134(11)

Published: Oct. 30, 2023

Microbial biocontrol agents have become an effective option to mitigate the harmfulness of chemical pesticides in recent years. This study demonstrates control efficacy Bacillus velezensis CE 100 on anthracnose causal agent, Colletotrichum gloeosporioides.In vitro antifungal assays revealed that culture filtrate and volatile organic compounds B. strongly restricted mycelial development C. gloeosporioides. Moreover, a bioactive compound, butyl succinate, was isolated from n-butanol crude extract (bce), identified by liquid chromatography-electrospray ionization hybrid ion-trap time-of-flight mass spectrometry (LC-ESI-QTOF-MS) one-dimensional (1D) two-dimensional (2D) nuclear magnetic resonance (NMR). Treatment with purified succinate at concentration 300 μg mL-1 controlled conidial germination gloeosporioides inhibition rate 98.66%, whereas 400 showed weak action growth 31.25%. Scanning electron microscopy morphologies succinate-treated hyphae conidia were severely deformed shriveled wrinkled surfaces. Furthermore, able carbendazim-resistant gloeosporioides, demonstrating it could be promising agent for suppression other fungal pathogens. An vivo assay demonstrated strain ce broth higher apple than bce.Our findings provide insight into potential its efficient phytopathogenic fungi, such as gloeosporiodes, plant disease protection. is first demonstrate bacteria-derived

Language: Английский

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Growth Enhancement of Tomato by a Plant Growth Promoting Bacterium, Bacillus subtilis PE7

Korean Journal of Soil Science and Fertilizer, Journal Year: 2023, Volume and Issue: 56(4), P. 398 - 406

Published: Nov. 30, 2023

Bacillus species are well recognized as effective bio-stimulants to enhance crop quality and productivity. Our study demonstrates the effect of a plant growth promoting bacterium, subtilis PE7 on tomato growth. In vitro screening assay for traits indicated that indole-3-acetic acid (IAA) production B. in terms increasing intensity pink color with concentration ⳑ-tryptophan supplemented tryptone soy broth (TSB) medium. The supplementation (1 g L-1) TSB medium resulted maximum IAA strain 25.59 µg mL-1 at 6 days after incubation. Moreover, highest ammonia 21.22 was observed an incubation period 72 h. results our pot experiment revealed treatment culture (inoculated fertilizer-based medium) induced significant promotion plants resulting higher values leaf number, length, fresh dry weights shoot compared control fertilizer treatment. Therefore, could be considered candidate development eco-friendly commercial production.Growth by 5 weeks transplantation. *Con: water control, F: treatment, PE7: culture.

Language: Английский

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Antifungal Substances Produced by B. subtilis Strain W3.15 Inhibit the Fusarium oxysporum and Trigger Cellular Damage

HAYATI Journal of Biosciences, Journal Year: 2023, Volume and Issue: 30(5), P. 843 - 854

Published: June 12, 2023

Soybean Fusarium wilt and root rot disease caused by a necrotrophic ascomycete pathogen, F. oxysporum, triggered severe damage to the plant tissues organs impacted heavy losses. Biocontrol agents, Bacillus subtilis, were commonly used produce broad spectrum of antifungal substances gradually in biocontrol studies for management. Investigation determination inhibiting mechanism substance produced B. subtilis on oxysporum should be done protect soybean plant. This study revealed that basal nutrient broth (NB) gives best activity. The stationary phase bacterial growth curve was obtained two days cultivation showed maximum activity against oxysporum. Ethyl acetate (EA) extraction supernatant generated crude EA extract, which half inhibition (IC50) at 306.42 µg/ml from dose-response regression curve. Post-treatment mycelia with extract demonstrated as hyphal deformation followed malondialdehyde (MDA) accumulation. Furthermore, cellular leakage fungal cells may compounds strain W3.15 occurred. Last, related predicted epicatechin benzophenone LC-MS/MS analysis extract. Accordingly, agent promises strong potency biofungicide development.

Language: Английский

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