Bacillus velezensis BVE7 as a promising agent for biocontrol of soybean root rot caused by Fusarium oxysporum

Frontiers in Microbiology, Journal Year: 2023, Volume and Issue: 14

Published: Oct. 20, 2023

Soybean root rot (SRR), caused by Fusarium oxysporum, is a severe soil-borne disease in soybean production worldwide, which adversely impacts the yield and quality of soybean. The most effective method for managing crop diseases decreasing reliance on chemical fungicides, such as Bacillus spp., via microbial biocontrol agents.In this study, soil-isolated strain BVE7 was identified B. velezensis, exhibiting broad-spectrum activity against various pathogens causing rot. sterile filtrate, at concentration 10%, demonstrated significant antifungal inhibiting conidial germination, production, mycelial growth F. oxysporum 61.11%, 73.44%, 85.42%, respectively, hyphal malformations. compound produced adaptability to standard environment. pot experiment showed that suspension could effectively control rot, with highest efficiency 75.13%. Furthermore, it considerably enhanced catalase, phenylalanine ammonia lyase, superoxide dismutase, peroxidase roots, while also preventing an increase malondialdehyde activity. By improving host resistance towards pathogens, damage fungi severity have been reduced.This study presents innovative utilization isolated from roots cold conditions, controlling oxysporum. findings highlight remarkable regional adaptive characteristics strain, making excellent candidate combating diverse environments. In conclusion, velezensis potential reducing SRR incidence can be considered viable option management.

Language: Английский

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Genomic and metabolomic insights into the antimicrobial compounds and plant growth-promoting potential of Bacillus velezensis B115

Scientific Reports, Journal Year: 2025, Volume and Issue: 15(1)

Published: March 28, 2025

The B115 strain, isolated from the inter-root soil of healthy plants in a continuous cropping site Panax notoginseng, was identified as Bacillus velezensis by 16S rDNA sequence comparison and comparative genomic analysis. is strain beneficial microorganisms present zone plants, with favorable plant growth-promoting properties antagonistic effects against pathogen Fusarium oxysporum. However, whole genome remains unclear, thus restricting its potential applications. To address this gap, has been sequenced annotated to elucidate molecular mechanisms underlying antimicrobial activities. analysis revealed that comprises single circular chromosome 4,200,774 bp plasmid region 16,878 long, possessing GC content 45.95%. Moreover, 4349 protein-coding genes were predicted. Notably, contains substantial number (103) involved biosynthesis, transport, catabolism secondary metabolites. Through mining, 13 BGCs 540 encoding metabolites predicted roles identified, including members surfactin fengycin families. Utilizing LC–MS/MS technologies, 2318 detected fermentation broth B. B115, encompassing compounds such Corynebactin, Gamabufotalin, Pracinostat, Indoleacetic acid, (8)-Gingerol, Luteolin, Liquiritigenin, other antimicrobial, growth-promoting, antioxidant, antitumor properties. By exploring metabolite-related predicting based on whole-genome results, we further basis for ability promote growth inhibit activity.

Language: Английский

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Eco-Friendly Biomass Production and Identification of Active Compounds of Paenibacillus polymyxa EB.KN35 with Potent Anti-Fusarium oxysporum Effect

Microorganisms, Journal Year: 2025, Volume and Issue: 13(4), P. 800 - 800

Published: March 31, 2025

Fusarium oxysporum is a fungal plant pathogen for over 100 agricultural crop species. There are strategies managing wilt, including antagonistic bacteria that offer promising and sustainable effect. In this work, among the various endophytic bacterial strains, Paenibacillus polymyxa EB.KN35 was selected as best antifungal strain against F. oxysporum. For eco-friendly biomass production of bacterium, some byproducts were tested cultivation, soybean processing byproduct (SPBP) found to be suitable C/N source P. fermentation. The utilization 14 L bioreactor system fermentation achieved high productivity (3.46 × 1011 CFU/mL) in short time (8 h). bioactive compound analysis, secreting several growth-promoting compounds such GA3, IAA, kinetin, zeatin (via HPLC) eleven volatile GC–MS). docking study indicated (1, 2, 4, 9) may play significant role inhibiting results highlight potential reusing bioproduction with use biocontrol agent biofertilizer.

Language: Английский

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Regional fingerprint, enrichment and flavor contribution of Bacillus community in Daqu for Chinese Baijiu

Food Research International, Journal Year: 2025, Volume and Issue: 211, P. 116460 - 116460

Published: April 19, 2025

Language: Английский

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A comparative metabolomics study of Panax ginseng infected by Fusarium oxysporum

Journal of Plant Pathology, Journal Year: 2025, Volume and Issue: unknown

Published: May 8, 2025

Language: Английский

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Protective role of Mortierella alpina-derived lipids in resisting root rot in Panax ginseng

Rhizosphere, Journal Year: 2024, Volume and Issue: unknown, P. 100994 - 100994

Published: Nov. 1, 2024

Language: Английский

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Strong Opponent of Walnut Anthracnose—Bacillus velezensis and Its Transcriptome Analysis

Microorganisms, Journal Year: 2023, Volume and Issue: 11(8), P. 1885 - 1885

Published: July 26, 2023

Walnut is a significant economic tree species worldwide. anthracnose, caused by the pathogen Colletotrichum gloeosporioides, greatly reduces walnut production and benefits. Our study showed that Bacillus velezensis effectively halted growth of C. inducing noticeable abnormalities such as hyphal breakage distortion, thereby curtailing pathogen’s virulence. A 50–100 times dilution B. fermentation broth, applied every two to three days, served an efficient protective layer for leaves fruits against gloeosporioides infection. Transcriptomic analysis unveiled its dynamic response gloeosporioides. On second day, upregulated number differentially expressed genes related synthesis metabolic products, amino acid biosynthesis, motility. fourth continuous products acids, along with differential expression spore-related genes, was observed. By sixth focus shifted towards environmental adaptation carbon source utilization. Throughout process, likely employed strategies release increased chemotaxis, nutrient competition exert antagonistic effect on Fluorescence quantitative results 15 primer pairs were up-regulated down-regulated, 100% similarity rate transcriptome sequencing results, confirming their authenticity. These findings provided foundation widespread application biocontrol agent in agriculture forestry.

Language: Английский

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Unlocking plant defenses: Harnessing the power of beneficial microorganisms for induced systemic resistance in vegetables – A systematic review

Biological Control, Journal Year: 2023, Volume and Issue: 188, P. 105428 - 105428

Published: Dec. 16, 2023

Plants are susceptible to pathogen attacks, which employ strategies that overcome plant defenses and result in damage losses. The use of beneficial microorganisms can induce systemic resistance, leading greater efficiency disease control. objective this study was identify the main resistance genes metabolites involved process. A bibliographic search conducted on Scopus Web Science platforms, metadata were evaluated using Bibliometrix package R software. Subsequently, an investigation carried out their interactions with gene expression defense systems. In studies induced most cited Trichoderma, Bacillus, Pseudomonas, used Solanum lycopersicon L. followed by Arabidopsis thaliana. Among pathogens, employed Botrytis cinerea, Pseudomonas syringae, Rhizoctonia solani, Fusarium oxysporum. Beneficial influence responsible for signaling jasmonic acid, salicylic ethylene, production enzymes complex reactive oxygen species as mediators protection.

Language: Английский

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Biological Control Potential of Bacillus subtilis Isolate 1JN2 against Fusarium Wilt on Cucumber

Horticulturae, Journal Year: 2024, Volume and Issue: 10(8), P. 843 - 843

Published: Aug. 9, 2024

Cucumber is one of the top ten vegetables globally and widely cultivated worldwide. However, Fusarium wilt, caused by oxysporum f. sp. Cucumerinum, most serious soil-borne diseases in cucumber cultivation, causing significant economic losses. Biological control has great potential prevention wilt disease, but mechanism involved still needs further research. In this study, biocontrol isolate Bacillus subtilis 1JN2, which was isolated our previous work, evaluated field conditions against rhizosphere fungal diversity analyzed. The results indicated that efficacy B. 1JN2 reached 58.5% compared with blank control, population density F. decreased from 495 copies/g soil before inoculation to 20 14 days after treatment. High-throughput sequencing demonstrated an populations significantly include genera Olpidium Pseudallescheria, more than 20% less 8%. And increased belonged family Chaetomiaceae, 6.82% 18.77%, 12.39%, 44.41%, 19.41% at four sample time points addition, soil-related enzyme activities, including catalase, dehydrogenase, alkaline phosphatase, polyphenol oxidase, were analyzed treatment 1JN2. all activities showed upward trend following inoculation. These findings demonstrate using as a agent for controlling cucumber.

Language: Английский

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Antifungal Peptide P852 Effectively Controls Fusarium oxysporum, a Wilt-Causing Fungus, by Affecting the Glucose Metabolism and Amino Acid Metabolism as well as Damaging Mitochondrial Function

Journal of Agricultural and Food Chemistry, Journal Year: 2023, Volume and Issue: 71(49), P. 19638 - 19651

Published: Nov. 28, 2023

Fusarium oxysporum causes wilt disease, which huge economic losses to a wide range of agricultural cash crops. Antifungal peptide P852 is an effective biocide. However, the mechanism direct inhibition pathogenic fungus needs be explored. The proteomics and transcriptomics results showed that mainly affected intracellular pathways such as glucose metabolism, amino acid oxidoreductase activity in F. oxysporum. disrupts oxidative equilibrium oxysporum, transmission electron microscopy observed mitochondrial swelling, disruption membrane structure, leakage contents. Decreased potential, cytochrome c leakage, reduced ATP production were also detected. These suggest able simultaneously inhibit metabolism disrupt function exerting its inhibitory effects multiple together. present study provides some insights into multitargeted antifungal lipopeptide substances produced by Bacillus spp.

Language: Английский

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