Springer series on biofilms, Journal Year: 2024, Volume and Issue: unknown, P. 43 - 90
Published: Jan. 1, 2024
Language: Английский
Antibiotics, Journal Year: 2023, Volume and Issue: 12(6), P. 1005 - 1005
Published: June 3, 2023
Biofilms are intricate multicellular structures created by microorganisms on living (biotic) or nonliving (abiotic) surfaces. Medically, biofilms often lead to persistent infections, increased antibiotic resistance, and recurrence of infections. In this review, we highlighted the clinical problem associated with biofilm infections focused current emerging antibiofilm strategies. These strategies directed at disrupting quorum sensing, which is crucial for formation, preventing bacterial adhesion surfaces, impeding aggregation in viscous mucus layers, degrading extracellular polymeric matrix, developing nanoparticle-based antimicrobial drug complexes target cells within core. It important acknowledge, however, that use agents faces obstacles, such as limited effectiveness vivo, potential cytotoxicity host cells, propensity elicit resistance targeted biofilm-forming microbes. Emerging next generation strategies, rely multipronged approaches, were highlighted, these benefit from advances nanotechnology, synthetic biology, discovery. The assessment mitigation presented here, could guide future initiatives toward innovative therapeutic Enhancing efficacy specificity some via careful investigations, under conditions closely mimic characteristics human body, bridge gap between laboratory research practical application.
Language: Английский
Citations
46
BMC Microbiology, Journal Year: 2023, Volume and Issue: 23(1)
Published: March 30, 2023
Abstract The opportunistic human pathogen Pseudomonas aeruginosa is the causal agent of a wide variety infections. This non-fermentative Gram-negative bacillus can colonize zones where skin barrier weakened, such as wounds or burns. It also causes infections urinary tract, respiratory system bloodstream. P. are common in hospitalized patients for which multidrug-resistant, respectively extensively drug-resistant isolates be strong contributor to high rate in-hospital mortality. Moreover, chronic cystic fibrosis especially concerning, since very tedious treat. exploits diverse cell-associated and secreted virulence factors, play essential roles its pathogenesis. Those factors encompass carbohydrate-binding proteins, quorum sensing that monitor production extracellular products, genes conferring extensive drug resistance, secretion deliver effectors kill competitors subvert host functions. In this article, we highlight recent advances understanding pathogenicity well efforts identification new targets development therapeutic strategies against These provide innovative promising circumvent infection caused by important pathogen.
Language: Английский
Citations
44
Biology, Journal Year: 2025, Volume and Issue: 14(2), P. 165 - 165
Published: Feb. 6, 2025
Healthcare-associated infections pose a significant global health challenge, negatively impacting patient outcomes and burdening healthcare systems. A major contributing factor to healthcare-associated is the formation of biofilms, structured microbial communities encased in self-produced extracellular polymeric substance matrix. Biofilms are critical disease etiology antibiotic resistance, complicating treatment infection control efforts. Their inherent resistance mechanisms enable them withstand therapies, leading recurrent increased morbidity. This review explores development biofilms their dual roles disease. It highlights structural protective functions EPS matrix, which shields populations from immune responses antimicrobial agents. Key molecular biofilm including restricted penetration, persister cell dormancy, genetic adaptations, identified as barriers effective management. implicated various clinical contexts, chronic wounds, medical device-associated infections, oral complications, surgical site infections. prevalence hospital environments exacerbates challenges underscores urgent need for innovative preventive therapeutic strategies. evaluates cutting-edge approaches such DNase-mediated disruption, RNAIII-inhibiting peptides, DNABII proteins, bacteriophage nanoparticle-based solutions, coatings, lock therapies. also examines associated with biofilm-related diagnostic difficulties, disinfectant economic implications. emphasizes multidisciplinary approach importance understanding dynamics, role pathogenesis, advancements strategies combat biofilm-associated effectively settings. These insights aim enhance reduce burden diseases.
Language: Английский
Citations
2
Critical Care, Journal Year: 2025, Volume and Issue: 29(1)
Published: Feb. 26, 2025
In critical illness, all elements of gut function are perturbed. Dysbiosis develops as the microbial community loses taxonomic diversity and new virulence factors appear. Intestinal permeability increases, allowing for translocation bacteria and/or bacterial products. Epithelial is altered at a cellular level homeostasis epithelial monolayer compromised by increased intestinal cell death decreased proliferation. Gut immunity impaired with simultaneous activation maladaptive pro- anti-inflammatory signals leading to both tissue damage susceptibility infections. Additionally, splanchnic vasoconstriction leads blood flow local ischemic changes. Together, these interrelated gastrointestinal dysfunction drive then perpetuate multi-organ syndrome. Despite clear importance maintaining homeostasis, there very few reliable measures in illness. Further, while multiple therapeutic strategies have been proposed, most not shown conclusively demonstrate benefit, care still largely supportive. The key role illness was subject tenth Perioperative Quality Initiative meeting, conference summarize current state literature identify knowledge gaps future study. This review product that conference.
Language: Английский
Citations
2
npj Antimicrobials and Resistance, Journal Year: 2023, Volume and Issue: 1(1)
Published: Sept. 22, 2023
Abstract The remarkable impact of antibiotics on human health is being eroded at an alarming rate by the emergence multidrug resistant pathogens. There a recognised consensus that new strategies to tackle infection are urgently needed limit devasting antibiotic resistance our global healthcare infrastructure. Next generation antimicrobials (NGAs) compounds target bacterial virulence factors disrupt pathogenic potential without impacting viability. By disabling key required establish and maintain infection, NGAs make pathogens more vulnerable clearance immune system can potentially render them susceptible traditional antibiotics. In this review, we discuss developing field how advancements in area could offer viable standalone alternative or effective means prolong efficacy when used combination.
Language: Английский
Citations
35
Biomedicines, Journal Year: 2023, Volume and Issue: 11(3), P. 765 - 765
Published: March 2, 2023
Sepsis is a life-threatening condition characterized by an uncontrolled inflammatory response to infectious agent and its antigens. Immune cell activation against the antigens causes severe distress that mediates strong in vital organs. responsible for high rate of morbidity mortality immunosuppressed patients. Monoclonal antibody (mAb)-based therapeutic strategies are now being explored as viable therapy option sepsis septic shock. antibodies may provide benefits through two major strategies: (a) monoclonal targeting pathogen components, (b) mAbs signaling directly suppress production mediators. The focus mAb therapies has been bacterial endotoxin (lipopolysaccharide), although other surface also investigated therapy. Several promising candidates undergoing clinical trials at present. Despite several failures investigation novel targets, provides glimmer hope treatment In this review, candidates, their efficacy controlling infection, with special emphasis on potential roadblocks, prospects discussed.
Language: Английский
Citations
26
International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(10), P. 5487 - 5487
Published: May 17, 2024
Antimicrobial resistance (AMR) is one of the most critical threats to global public health in 21st century, causing a large number deaths every year both high-income and low- middle-income countries. Vaccines monoclonal antibodies can be exploited prevent treat diseases caused by AMR pathogens, thereby reducing antibiotic use decreasing selective pressure that favors emergence resistant strains. Here, differences mechanism action vaccines compared antibiotics are discussed. The state art for vaccine technologies reviewed, with particular focus on approaches validated clinical studies. By underscoring scope limitations different emerging technologies, this review points out complementary fighting AMR. Gaps antigen discovery some as well challenges associated development these therapies against highlighted.
Language: Английский
Citations
9
Expert Opinion on Therapeutic Patents, Journal Year: 2024, Volume and Issue: 34(6), P. 493 - 509
Published: April 29, 2024
Introduction Pseudomonas aeruginosa (PA) is a Gram-negative bacterium that can cause wide range of severe infections in immunocompromised patients. The most difficult challenge due to its ability rapidly develop multi drug-resistance. New strategies are urgently required improve the outcome patients with PA infections. present patent review highlights new molecules acting on different targets involved antibiotic resistance.
Language: Английский
Citations
5
Infection and Drug Resistance, Journal Year: 2023, Volume and Issue: Volume 16, P. 5359 - 5373
Published: Aug. 1, 2023
Abstract: Haemophilus influenzae ( H. ) is a significant pathogen responsible for causing respiratory tract infections and invasive diseases, leading to considerable disease burden. The type b (Hib) conjugate vaccine has notably decreased the incidence of severe caused by Hib strains, other non-typable (NTHi) serotypes have emerged as epidemic strains worldwide. As result, global trends antibiotic resistance characteristics been altered. Researches on virulence factors , particularly mechanisms underlying biofilm formation, development anti-biofilm strategies hold clinical value. This article provides summary trends, typing methods, factors, formation mechanisms, prevention . increasing prevalence NTHi among especially high β-lactamase positivity emergence BLNAR increased difficulties. Understanding its mechanism biofilm, formulating effective may help reduce impact. Therefore, future research efforts should focus developing new approaches prevent control infections. Keywords: epidemiology, biofilms,
Language: Английский
Citations
9
Scientific Reports, Journal Year: 2023, Volume and Issue: 13(1)
Published: Aug. 10, 2023
Abstract Bacterial biofilms contribute significantly to pathogenesis, recurrence and/or chronicity of the majority bacterial diseases due their notable recalcitrance clearance. Herein, we examined kinetics enhanced sensitivity nontypeable Haemophilus influenzae (NTHI) newly released (NRel) from biofilm residence by a monoclonal antibody against DNABII protein (α-DNABII) preferential killing β-lactam antibiotic. This phenotype was detected within 5 min and lasted for ~ 6 h. Relative expression genes selected known involvement in showed transient up-regulated penicillin binding proteins α-DNABII NTHI NRel, whereas there limited β-lactamase precursor. Transient down-regulated mediators oxidative stress supported similarly timed vulnerability NADPH-oxidase sensitive intracellular activated human PMNs. Further, major porin aligned well with observed increased membrane permeability characteristic also shown NRel three additional pathogens. These data provide mechanistic insights as transient, yet highly vulnerable, phenotype. heightened understanding supports continued validation this novel therapeutic approach designed leverage knowledge more effective eradication recalcitrant biofilm-related diseases.
Language: Английский
Citations
4