Articular cartilage repair biomaterials: strategies and applications

Materials Today Bio, Journal Year: 2024, Volume and Issue: 24, P. 100948 - 100948

Published: Jan. 5, 2024

Articular cartilage injury is a frequent worldwide disease, while effective treatment urgently needed. Due to lack of blood vessels and nerves, the ability self-repair limited. Despite availability various clinical treatments, unfavorable prognoses complications remain prevalent. However, advent tissue engineering regenerative medicine has generated considerable interests in using biomaterials for articular repair. Nevertheless, there remains notable scarcity comprehensive reviews that provide an in-depth exploration strategies applications. Herein, we present overview primary bioactive substances from perspective repair cartilage. The include regeneration, substitution, immunization. We comprehensively delineate influence mechanically supportive scaffolds on cellular behavior, shedding light emerging scaffold technologies, including stimuli-responsive smart scaffolds, 3D-printed bionic scaffolds. Biologically active substances, factors, stem cells, extracellular vesicles (EVs), organoids, are elucidated their roles regulating activity chondrocytes. Furthermore, composite produced industrially put into use, also explicitly presented. This review offers innovative solutions treating ailments emphasizes potential translation.

Language: Английский

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Multiphasic scaffolds for the repair of osteochondral defects: Outcomes of preclinical studies

Bioactive Materials, Journal Year: 2023, Volume and Issue: 27, P. 505 - 545

Published: April 28, 2023

Osteochondral defects are caused by injury to both the articular cartilage and subchondral bone within skeletal joints. They can lead irreversible joint damage increase risk of progression osteoarthritis. Current treatments for osteochondral injuries not curative only target symptoms, highlighting need a tissue engineering solution. Scaffold-based approaches be used assist regeneration, where biomaterials tailored properties restore defect minimise further degeneration. This review captures original research studies published since 2015, on multiphasic scaffolds treat in animal models. These an extensive range scaffold fabrication, consisting mainly natural synthetic polymers. Different methods were create designs, including integrating or fabricating multiple layers, creating gradients, through addition factors such as minerals, growth factors, cells. The variety animals model defects, rabbits most commonly chosen vast majority reported small rather than large few available clinical reporting cell-free have shown promising early-stage results repair, but long-term follow-up is necessary demonstrate consistency restoration. Overall, preclinical show favourable simultaneously regenerating models suggesting that biomaterials-based strategies may

Language: Английский

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DNA-encoded Dynamic Hydrogels for 3D Bioprinted Cartilage Organoids

Materials Today Bio, Journal Year: 2025, Volume and Issue: 31, P. 101509 - 101509

Published: Jan. 22, 2025

Articular cartilage, composed of chondrocytes within a dynamic viscoelastic matrix, has limited self-repair capacity, posing significant challenge for regeneration. Constructing high-fidelity cartilage organoids through three-dimensional (3D) bioprinting to replicate the structure and physiological functions is crucial regenerative medicine, drug screening, disease modeling. However, commonly used matrix bioinks lack reversible cross-linking precise controllability, hindering cellular regulation. Thus, encoding adaptive cultivating an attractive idea. DNA, with its ability be intricately encoded reversibly cross-linked into hydrogels, offers manipulation at both molecular spatial structural levels. This endows hydrogels viscoelasticity, printability, cell recognition, stimuli responsiveness. paper elaborates on strategies encode bioink via emphasizing regulation predictable properties resulting interactions behavior. The significance these construction highlighted. Finally, we discuss challenges future prospects using DNA-encoded 3D bioprinted organoids, underscoring their potential impact advancing biomedical applications.

Language: Английский

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Microcarriers in application for cartilage tissue engineering: Recent progress and challenges

Bioactive Materials, Journal Year: 2022, Volume and Issue: 17, P. 81 - 108

Published: Jan. 25, 2022

Successful regeneration of cartilage tissue at a clinical scale has been tremendous challenge in the past decades. Microcarriers (MCs), usually used for cell and drug delivery, have studied broadly across wide range medical fields, especially engineering (TE). Notably, microcarrier systems provide an attractive method regulating phenotype microtissue maturations, they also serve as powerful injectable carriers are combined with new technologies regeneration. In this review, we introduced typical methods to fabricate various types microcarriers discussed appropriate materials microcarriers. Furthermore, highlighted recent progress applications general design principle Finally, summarized current challenges promising prospects microcarrier-based applications. Overall, review provides comprehensive systematic guidelines rational TE.

Language: Английский

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Spatial patterning of phenotypically distinct microtissues to engineer osteochondral grafts for biological joint resurfacing

Biomaterials, Journal Year: 2022, Volume and Issue: 289, P. 121750 - 121750

Published: Aug. 28, 2022

Modular biofabrication strategies using microtissues or organoids as biological building blocks have great potential for engineering replacement tissues and organs at scale. Here we describe the development of a strategy to engineer osteochondral by spatially localising phenotypically distinct cartilage within an instructive 3D printed polymer framework. We first demonstrate that immature can spontaneously fuse form homogeneous macrotissues, combining less cellular results in superior fusion generation more hyaline-like containing higher levels sulphated glycosaminoglycans type II collagen. Furthermore, temporally exposing developing transforming growth factor-β accelerates their volumetric subsequent capacity into larger hyaline grafts. Next, polymeric frameworks are used further guide microtissue self-organisation process, resulting macroscale tissue with zonal collagen organisation analogous structure seen native articular cartilage. To grafts, hypertrophic engineered bone precursor localised below stable microtissues. Implantation these grafts critically-sized caprine defects effective defect stabilisation histologically supports restoration normal surface after 6 months vivo. These findings support use such modular joint resurfacing.

Language: Английский

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Dually crosslinked injectable alginate-based graft copolymer thermoresponsive hydrogels as 3D printing bioinks for cell spheroid growth and release

Carbohydrate Polymers, Journal Year: 2023, Volume and Issue: 312, P. 120790 - 120790

Published: March 10, 2023

In this work a dual crosslinked network based on sodium alginate graft copolymer, bearing poly(N-isopropylacrylamide-co-N-tert-butylacrylamide) P(NIPAM-co-NtBAM) side chains was developed and examined as shear thinning soft gelating bioink. The copolymer found to undergo two-step gelation mechanism; in the first step three-dimensional (3D) is formed through ionic interactions between negatively ionized carboxylic groups of backbone positive charges Ca2+ divalent cations, according "egg-box" mechanism. second occurs upon heating which triggers hydrophobic association thermoresponsive chains, increasing crosslinking density highly cooperative manner. Interestingly, mechanism resulted five-to-eight-fold improvement storage modulus implying reinforced above critical thermo-gelation temperature further boosted by backbone. proposed bioink could form arbitrary geometries under mild 3D printing conditions. Last, it demonstrated that can be utilized bioprinting ink showcased its ability promote human periosteum derived cells (hPDCs) growth their capacity spheroids. conclusion, bioink, owing reverse thermally polymer network, for facile recovery cell spheroids, promising potential use spheroid-forming template bionk applications biofabrication.

Language: Английский

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In situ self-assembled organoid for osteochondral tissue regeneration with dual functional units

Bioactive Materials, Journal Year: 2023, Volume and Issue: 27, P. 200 - 215

Published: April 10, 2023

The regeneration of hierarchical osteochondral units is challenging due to difficulties in inducing spatial, directional and controllable differentiation mesenchymal stem cells (MSCs) into cartilage bone compartments. Emerging organoid technology offers new opportunities for regeneration. In this study, we developed gelatin-based microcryogels customized using hyaluronic acid (HA) hydroxyapatite (HYP), respectively (denoted as CH-Microcryogels OS-Microcryogels) through vivo self-assembly organoids. showed good cytocompatibility induced chondrogenic osteogenic MSCs, while also demonstrating the ability self-assemble organoids with no delamination biphasic cartilage-bone structure. Analysis by mRNA-seq that promoted inhibited inflammation, OS-Microcryogels facilitated suppressed immune response, regulating specific signaling pathways. Finally, engraftment pre-differentiated canine defects resulted spontaneous assembly an unit, simultaneous both articular subchondral bone. conclusion, novel approach generating self-assembling utilizing tailor-made presents a highly promising avenue advancing field tissue engineering.

Language: Английский

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Global Literature Analysis of Organoid and Organ‐on‐Chip Research

Advanced Healthcare Materials, Journal Year: 2023, Volume and Issue: 13(21)

Published: July 22, 2023

Organoids and cells in organ-on-chip platforms replicate higher-level anatomical, physiological, or pathological states of tissues organs. These technologies are widely regarded by academia, the pharmacological industry regulators as key biomedical developments. To map advances this emerging field, a literature analysis 16,000 article metadata based on quality-controlled text-mining algorithm is performed. The covers titles, keywords, abstracts categorized academic publications preprint databases published after 2010. identifies tracks 149 107 organs organ substructures modeled organoids organ-on-chip, respectively, stem cell sources, well 130 diseases, 16 groups organisms other than human mouse which organoid/organ-on-chip technology applied. illustrates changing diversity focus research captures its geographical distribution. downloadable dataset provided robust framework for researchers to interrogate with their own questions.

Language: Английский

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Effects of Electrical Stimulation on Articular Cartilage Regeneration with a Focus on Piezoelectric Biomaterials for Articular Cartilage Tissue Repair and Engineering

International Journal of Molecular Sciences, Journal Year: 2023, Volume and Issue: 24(3), P. 1836 - 1836

Published: Jan. 17, 2023

There is increasing evidence that chondrocytes within articular cartilage are affected by endogenous force-related electrical potentials. Furthermore, stimulation (ES) promotes the proliferation of and synthesis extracellular matrix (ECM) molecules, which accelerate healing defects. These findings suggest potential application ES in repair. In this review, we summarize pathogenesis injuries current clinical strategies for treatment injuries. We then focus on repair vivo. The ES-induced chondrogenic differentiation mesenchymal stem cells (MSCs) its regulatory mechanism discussed detail. addition, discuss applying piezoelectric materials process constructing engineering cartilage, highlighting important advances unique field tissue engineering.

Language: Английский

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Engineering bone/cartilage organoids: strategy, progress, and application

Bone Research, Journal Year: 2024, Volume and Issue: 12(1)

Published: Nov. 20, 2024

Abstract The concept and development of bone/cartilage organoids are rapidly gaining momentum, providing opportunities for both fundamental translational research in bone biology. Bone/cartilage organoids, essentially miniature tissues grown vitro, enable the study complex cellular interactions, biological processes, disease pathology a representative controlled environment. This review provides comprehensive up-to-date overview field, focusing on strategies organoid construction strategies, progresses research, potential applications. We delve into significance selecting appropriate cells, matrix gels, cytokines/inducers, techniques. Moreover, we explore role advancing our understanding reconstruction, modeling, drug screening, prevention, treatment strategies. While acknowledging these discuss inherent challenges limitations field propose solutions, including use bioprinting induction, AI improved screening exploration assembloids more complex, multicellular models. believe that with continuous refinement standardization, can profoundly impact patient-specific therapeutic interventions lead way regenerative medicine.

Language: Английский

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