Biomimetic-smart materials for osteochondral regeneration and repair DOI Open Access

Qi-Peng Jia,

Qunfeng Li,

Hamza Boucetta

et al.

Microstructures, Journal Year: 2024, Volume and Issue: 4(3)

Published: May 14, 2024

Osteochondral injuries represent prevalent clinical conditions with profound implications for functional impairment and diminished quality of life. Despite the considerable potential tissue engineering in osteochondral repair, substantial strides implementation remain elusive. Biomimetic materials, designed to emulate natural cartilage, offer a stabilized structure microenvironment capable accommodating diverse properties inherent different cartilage regions. Smart endowed ability deliver drugs, metal ions, growth factors contingent on disease progression, exert precise control over cellular epigenetic behaviors. This review scrutinizes nuanced characteristics both physiological pathological states. Subsequently, succinct overview recent applications biomaterials bionic smart attributes regeneration repair is provided. Finally, we propose our perspectives application biomimetic-smart materials emphasizing their translation.

Language: Английский

AI-enabled organoids: Construction, analysis, and application DOI Creative Commons
Long Bai,

Yan Wu,

Guangfeng Li

et al.

Bioactive Materials, Journal Year: 2023, Volume and Issue: 31, P. 525 - 548

Published: Sept. 16, 2023

Organoids, miniature and simplified

Language: Английский

Citations

68
Boosting cartilage repair with silk fibroin-DNA hydrogel-based cartilage organoid precursor DOI Creative Commons

Congyi Shen,

Jian Wang, Guangfeng Li

et al.

Bioactive Materials, Journal Year: 2024, Volume and Issue: 35, P. 429 - 444

Published: Feb. 16, 2024

Osteoarthritis (OA), a common degenerative disease, is characterized by high disability and imposes substantial economic impacts on individuals society. Current clinical treatments remain inadequate for effectively managing OA. Organoids, miniature 3D tissue structures from directed differentiation of stem or progenitor cells, mimic native organ functions. They are useful drug testing serve as active grafts repair. However, organoid construction requires extracellular matrix-like scaffolds cellular growth. Hydrogel microspheres, with tunable physical chemical properties, show promise in cartilage engineering replicating the natural microenvironment. Building prior work SF-DNA dual-network hydrogels regeneration, we developed novel RGD-SF-DNA hydrogel microsphere (RSD-MS) via microfluidic system integrating photopolymerization self-assembly techniques then modified Pep-RGDfKA. The RSD-MSs exhibited uniform size, porous surface, optimal swelling degradation properties.

Language: Английский

Citations

53
Magnetic nanoparticles for ferroptosis cancer therapy with diagnostic imaging DOI Creative Commons
Min Jun Ko, Sunhong Min, Hyunsik Hong

et al.

Bioactive Materials, Journal Year: 2023, Volume and Issue: 32, P. 66 - 97

Published: Sept. 29, 2023

Ferroptosis offers a novel method for overcoming therapeutic resistance of cancers to conventional cancer treatment regimens. Its effective use as therapy requires precisely targeted approach, which can be facilitated by using nanoparticles and nanomedicine, their enhance ferroptosis is indeed growing area research. While few review papers have been published on iron-dependent mechanism inducers that partly covers nanoparticles, there need comprehensive focusing the design magnetic typically supply iron ions promote simultaneously enable nanomedicine. Furthermore, locally induce combinational with diagnostic resonance imaging (MRI). The remotely controllable nanocarriers offer highly localized image-guided Here, recent developments in magnetically manipulable nanomedicine medical are summarized. This also highlights advantages current state-of-the-art Finally, image guided apoptosis-based enables synergistic tumor discussed clinical translations.

Language: Английский

Citations

50
Research Progress in Hydrogels for Cartilage Organoids DOI

Xiaolong Li,

Shihao Sheng, Guangfeng Li

et al.

Advanced Healthcare Materials, Journal Year: 2024, Volume and Issue: 13(22)

Published: May 21, 2024

The repair and regeneration of cartilage has always been a hot topic in medical research. Cartilage organoids (CORGs) are special tissue created using engineering techniques outside the body. These engineered tissues provide models that simulate complex biological functions cartilage, opening new possibilities for regenerative medicine treatment strategies. However, it is crucial to establish suitable matrix scaffolds cultivation CORGs. In recent years, utilizing hydrogel culture stem cells induce their differentiation into chondrocytes emerged as promising method vitro construction this review, methods establishing CORGs summarized an overview advantages limitations matrigel such provided. Furthermore, importance ECM alternative substitutes Matrigel, alginate, peptides, silk fibroin, DNA derivatives discussed, pros cons these hydrogels outlined. Finally, challenges future directions research discussed. It hoped article provides valuable references design development

Language: Английский

Citations

23
Bone Organoids: Recent Advances and Future Challenges DOI
Ding Zhao, Qimanguli Saiding, Yihan Li

et al.

Advanced Healthcare Materials, Journal Year: 2023, Volume and Issue: 13(5)

Published: Dec. 12, 2023

Bone defects stemming from tumorous growths, traumatic events, and diverse conditions present a profound conundrum in clinical practice research. While bone has the inherent ability to regenerate, substantial anomalies require regeneration techniques. organoids represent new concept this field, involving 3D self-assembly of bone-associated stem cells guided vitro with or without extracellular matrix material, resulting tissue that mimics structural, functional, genetic properties native tissue. Within scientific panorama, ascend an esteemed status, securing significant experimental endorsement. Through synthesis current literature pioneering studies, review offers comprehensive survey organoid paradigm, delves into quintessential architecture ontogeny bone, highlights latest progress fabrication. Further, existing challenges prospective directions for future research are identified, advocating interdisciplinary collaboration fully harness potential burgeoning domain. Conclusively, as technology continues mature, its implications both landscapes poised be profound.

Language: Английский

Citations

26
Cartilage lacuna-biomimetic hydrogel microspheres endowed with integrated biological signal boost endogenous articular cartilage regeneration DOI Creative Commons
Hao Li, Tianyuan Zhao, Zhiguo Yuan

et al.

Bioactive Materials, Journal Year: 2024, Volume and Issue: 41, P. 61 - 82

Published: July 12, 2024

Despite numerous studies on chondrogenesis, the repair of cartilage-particularly reconstruction cartilage lacunae through an all-in-one advanced drug delivery system remains limited. In this study, we developed a lacuna-like hydrogel microsphere endowed with integrated biological signals, enabling sequential immunomodulation and endogenous articular regeneration. We first chondrogenic growth factor transforming factor-β3 (TGF-β3) into mesoporous silica nanoparticles (MSNs). Then, TGF-β3@MSNs insulin-like 1 (IGF-1) were encapsulated within microspheres made polydopamine (pDA). final step, factor-loaded MSN@pDA chitosan (CS) containing platelet-derived factor-BB (PDGF-BB) blended to produce factors loaded composite (GFs@μS) using microfluidic technology. The presence pDA reduced initial acute inflammatory response, early, robust release PDGF-BB aided in attracting stem cells. Over subsequent weeks, continuous IGF-1 TGF-β3 amplified chondrogenesis matrix formation. μS incorporated acellular extracellular (ACECM) combined polydopamine-modified polycaprolactone (PCL) structure tissue-engineered scaffold that mimicked evenly distributed matrix, resulting enhanced patellar protection. This research provides strategic pathway for optimizing ensuring prolonged microenvironmental remodeling, leading efficient

Language: Английский

Citations

11
Engineering bone/cartilage organoids: strategy, progress, and application DOI Creative Commons
Long Bai, Dongyang Zhou, Guangfeng Li

et al.

Bone Research, Journal Year: 2024, Volume and Issue: 12(1)

Published: Nov. 20, 2024

Abstract The concept and development of bone/cartilage organoids are rapidly gaining momentum, providing opportunities for both fundamental translational research in bone biology. Bone/cartilage organoids, essentially miniature tissues grown vitro, enable the study complex cellular interactions, biological processes, disease pathology a representative controlled environment. This review provides comprehensive up-to-date overview field, focusing on strategies organoid construction strategies, progresses research, potential applications. We delve into significance selecting appropriate cells, matrix gels, cytokines/inducers, techniques. Moreover, we explore role advancing our understanding reconstruction, modeling, drug screening, prevention, treatment strategies. While acknowledging these discuss inherent challenges limitations field propose solutions, including use bioprinting induction, AI improved screening exploration assembloids more complex, multicellular models. believe that with continuous refinement standardization, can profoundly impact patient-specific therapeutic interventions lead way regenerative medicine.

Language: Английский

Citations

9
DNA-Based Hydrogels for Bone Regeneration: A Promising Tool for Bone Organoids DOI Creative Commons
Xiang Wu, Yan Hu, Shihao Sheng

et al.

Materials Today Bio, Journal Year: 2025, Volume and Issue: 31, P. 101502 - 101502

Published: Jan. 19, 2025

DNA-based hydrogels stand out for bone regeneration due to their exceptional biocompatibility and programmability. These facilitate the formation of spatial structures through bulk hydrogel fabricating, microsphere formatting, 3D printing. Furthermore, microenvironment can be finely tuned by leveraging degradation products, nanostructure, targeting, delivery capabilities inherent materials. In this review, we underscore advantages hydrogels, detailing composition, gelation techniques, structure optimization. We then delineate three critical elements in promotion using hydrogels: (i) osteogenesis driven phosphate ions, plasmids, oligodeoxynucleotides (ODNs) that enhance mineralization promote gene protein expression; (ii) vascularization facilitated tetrahedral DNA nanostructures (TDNs) aptamers, which boosts expression targeted release; (iii) immunomodulation achieved loaded factors, TDNs, bound ions stimulate macrophage polarization exhibit antibacterial properties. With these properties, used construct organoids, providing an innovative tool disease modeling therapeutic applications tissue engineering. Finally, discuss current challenges future prospects, emphasizing potential impacts regenerative medicine.

Language: Английский

Citations

1
Exosomes derived from 3D-cultured MSCs alleviate knee osteoarthritis by promoting M2 macrophage polarization through miR-365a-5p and inhibiting TLR2/Myd88/NF-κB pathway DOI
Lei Yan,

Dijun Li,

Songyan Li

et al.

Chemical Engineering Journal, Journal Year: 2024, Volume and Issue: 497, P. 154432 - 154432

Published: July 30, 2024

Language: Английский

Citations

6
Cartilage organoids and osteoarthritis research: a narrative review DOI Creative Commons

Daofu Zeng,

Yeping Chen,

Zhidong Liao

et al.

Frontiers in Bioengineering and Biotechnology, Journal Year: 2023, Volume and Issue: 11

Published: Nov. 9, 2023

Osteoarthritis (OA) is one of the most common degenerative joint diseases, significantly impacting individuals and society. With acceleration global aging, incidence OA increasing. The pathogenesis osteoarthritis not fully understood, there no effective way to alleviate progression osteoarthritis. Therefore, it necessary develop new disease models seek treatments for OA. Cartilage organoids are three-dimensional tissue masses that can simulate organ structure physiological function play an important role in modeling, drug screening, regenerative medicine. This review will briefly analyze research progress OA, focusing on construction current development cartilage organoids, then describe application regeneration repair bone defects. Finally, some challenges prospects cartilaginous discussed.

Language: Английский

Citations

13