Osteoimmunity‐Regulating Biomimetically Hierarchical Scaffold for Augmented Bone Regeneration

Advanced Materials, Journal Year: 2022, Volume and Issue: 34(36)

Published: July 4, 2022

Engineering a proper immune response following biomaterial implantation is essential to bone tissue regeneration. Herein, biomimetically hierarchical scaffold composed of deferoxamine@poly(ε-caprolactone) nanoparticles (DFO@PCL NPs), manganese carbonyl (MnCO) nanosheets, gelatin methacryloyl hydrogel, and polylactide/hydroxyapatite (HA) matrix fabricated augment repair by facilitating the balance system metabolism. First, 3D printed stiff with well-organized gradient structure mimics cortical cancellous tissues; meanwhile, an inside infusion soft hydrogel further endows characteristics extracellular matrix. A Fenton-like reaction between MnCO endogenous hydrogen peroxide generated at implant-tissue site triggers continuous release carbon monoxide Mn2+ , thus significantly lessening inflammatory upregulating M2 phenotype macrophages, which also secretes vascular endothelial growth factor induce formation. Through activating hypoxia-inducible factor-1α pathway, DFO@PCL NP promote angiogenesis. Moreover, DFO inhibits osteoclast differentiation synergistically collaborates osteoinductive activity HA. Based on amounts data in vitro vivo, strong immunomodulatory, intensive angiogenic, weak osteoclastogenic, superior osteogenic abilities such osteoimmunity-regulating present profound effect improving regeneration, puts forward worthy base positive enlightenment for large-scale defect repair.

Language: Английский

---

Recent Advancements on Three-Dimensional Electrospun Nanofiber Scaffolds for Tissue Engineering

Advanced Fiber Materials, Journal Year: 2022, Volume and Issue: 4(5), P. 959 - 986

Published: May 16, 2022

Language: Английский

---

Scaffolds for bone-tissue engineering

Matter, Journal Year: 2022, Volume and Issue: 5(9), P. 2722 - 2759

Published: Sept. 1, 2022

Language: Английский

---

Smart‐Responsive Multifunctional Therapeutic System for Improved Regenerative Microenvironment and Accelerated Bone Regeneration via Mild Photothermal Therapy

Advanced Science, Journal Year: 2023, Volume and Issue: 11(2)

Published: Nov. 7, 2023

Abstract The treatment of bone defects remains a substantial clinical challenge due to the lack spatiotemporal management immune microenvironment, revascularization, and osteogenic differentiation. Herein, deferoxamine (DFO)‐loaded black phosphorus nanosheets decorated by polydopamine layer are prepared (BPPD) compounded into gelatin methacrylate/sodium alginate methacrylate (GA) hybrid hydrogel as smart‐responsive therapeutic system (GA/BPPD) for accelerated regeneration. BPPD nanocomposites served bioactive components near‐infrared (NIR) photothermal agents, which conferred with excellent NIR/pH dual‐responsive properties, realizing stimuli‐responsive release DFO PO 4 3 − during Under action NIR‐triggered mild therapy, GA/BPPD exhibited positive effect on promoting osteogenesis angiogenesis, eliminating excessive reactive oxygen species, inducing macrophage polarization M2 phenotype. More significantly, through polarization‐induced osteoimmune this platform could also drive functional cytokine secretion enhanced angiogenesis osteogenesis. In vivo experiments further demonstrated that facilitate healing attenuating local inflammatory response, increasing pro‐healing factors, stimulating endogenous cell recruitment, accelerating revascularization. Collectively, proposed intelligent provides promising strategy reshape damaged tissue microenvironment augmented

Language: Английский

---

Regulation of Osteoimmune Microenvironment and Osteogenesis by 3D‐Printed PLAG/black Phosphorus Scaffolds for Bone Regeneration

Advanced Science, Journal Year: 2023, Volume and Issue: 10(28)

Published: Aug. 24, 2023

The treatment of bone defects remains a significant challenge to be solved clinically. Immunomodulatory properties orthopedic biomaterials have significance in regulating osteoimmune microenvironment for osteogenesis. A lactic acid-co-glycolic acid (PLGA) scaffold incorporates black phosphorus (BP) fabricated by 3D printing technology investigate the effect BP on osteoimmunomodulation and osteogenesis site. PLGA/BP exhibits suitable biocompatibility, biodegradability, mechanical as an excellent support new formation. studies' result also demonstrate that scaffolds are able recruit stimulate macrophages M2 polarization, inhibit inflammation, promote human marrow mesenchymal stem cells (hBMSCs) proliferation differentiation, which turn promotes regeneration distal femoral defect region steroid-associated osteonecrosis (SAON) rat model. Moreover, it is screened demonstrated can osteogenic differentiation transcriptomic analysis, mineralization activating PI3K-AKT signaling pathway hBMSC cells. In this study, shown innovative extremely effective stimulating macrophage polarization strategy development used repair offered.

Language: Английский

---

Electrospun fiber‐based immune engineering in regenerative medicine

Smart Medicine, Journal Year: 2024, Volume and Issue: 3(1)

Published: Feb. 1, 2024

Abstract Immune engineering, a burgeoning field within regenerative medicine, involves spectrum of strategies to optimize the intricate interplay between tissue biomaterials and host tissue. These are applied across different types various disease models, which encompasses finely modulating immune response at levels cells factors, aiming mitigate adverse effects like fibrosis persistent inflammation that may arise injury site consequently promote regeneration. With continuous progress in electrospinning technology, immunoregulatory capabilities electrospun fibers have gained substantial attention over years. Electrospun fibers, with their extracellular matrix‐like characteristics, high surface‐area‐to‐volume ratio, reliable pharmaceutical compound capacity, emerged as key players among engineering materials. This review specifically focuses on role fiber‐based emphasizing unique design strategies. Notably, actively engages by responses through four essential strategies: (i) surface modification, (ii) drug loading, (iii) physicochemical parameters, (iv) biological grafting. presents comprehensive overview mechanisms system injured tissues while unveiling adopted orchestrate regulation. Furthermore, explores current developmental trends limitations concerning function drive advancements its full potential.

Language: Английский

---

The marriage of immunomodulatory, angiogenic, and osteogenic capabilities in a piezoelectric hydrogel tissue engineering scaffold for military medicine

Military Medical Research, Journal Year: 2023, Volume and Issue: 10(1)

Published: July 31, 2023

Most bone-related injuries to grassroots troops are caused by training or accidental injuries. To establish preventive measures reduce all kinds of trauma and improve the combat effectiveness troops, it is imperative develop new strategies scaffolds promote bone regeneration.

Language: Английский

---

Bioinspired soft-hard combined system with mild photothermal therapeutic activity promotes diabetic bone defect healing via synergetic effects of immune activation and angiogenesis

Theranostics, Journal Year: 2024, Volume and Issue: 14(10), P. 4014 - 4057

Published: Jan. 1, 2024

Background:The comprehensive management of diabetic bone defects remains a substantial clinical challenge due to the hostile regenerative microenvironment characterized by aggravated inflammation, excessive reactive oxygen species (ROS), bacterial infection, impaired angiogenesis, and unbalanced homeostasis.Thus, an advanced multifunctional therapeutic platform capable simultaneously achieving immune regulation, elimination, tissue regeneration is urgently designed for augmented under pathological milieu.Methods Results: Herein, photoactivated soft-hard combined scaffold system (PGCZ) was engineered introducing polydopamine-modified zeolitic imidazolate framework-8-loaded double-network hydrogel (soft matrix component) into 3D-printed poly(ε-caprolactone) (PCL) (hard component).The versatile PGCZ based on PCL thus prepared features highly extracellular matrix-mimicking microstructure, suitable biodegradability mechanical properties, excellent photothermal performance, allowing long-term structural stability support regeneration.Under periodic near-infrared (NIR) irradiation, localized effect triggers on-demand release Zn 2+ , which, together with repeated mild hyperthermia, collectively accelerates proliferation osteogenic differentiation preosteoblasts potently inhibits growth biofilm formation.Additionally, also presents outstanding immunomodulatory ROS scavenging capacities, which regulate M2 polarization macrophages drive functional cytokine secretion, leading pro-regenerative in situ enhanced vascularization.In vivo experiments further demonstrated that conjunction activity remarkably attenuated local inflammatory cascade, initiated endogenous stem cell recruitment neovascularization, orchestrated osteoblast/osteoclast balance, ultimately accelerating regeneration. Ivyspring

Language: Английский

---

Multifunctional dressings for wound exudate management

Progress in Materials Science, Journal Year: 2024, Volume and Issue: 146, P. 101328 - 101328

Published: June 14, 2024

Language: Английский

---

Emerging Roles of Macrophage Polarization in Osteoarthritis: Mechanisms and Therapeutic Strategies

Orthopaedic Surgery, Journal Year: 2024, Volume and Issue: 16(3), P. 532 - 550

Published: Jan. 31, 2024

Osteoarthritis (OA) is the most common chronic degenerative joint disease in middle‐aged and elderly people, characterized by pain dysfunction. Macrophages are key players OA pathology, their activation state has been studied extensively. Various studies have suggested that macrophages might respond to stimuli microenvironment changing phenotypes pro‐inflammatory or anti‐inflammatory phenotypes, which called macrophage polarization. accumulate become polarized (M1 M2) many tissues, such as synovium, adipose tissue, bone marrow, mesenchymal tissues joints, while resident well other stromal cells, including fibroblasts, chondrocytes, osteoblasts, form function an integrated unit. In this study, we focus exclusively on synovial macrophages, tissue osteoclasts, investigate roles development of OA. We review recent findings related polarization OA, pathogenesis, molecular pathways, therapeutics. summarize several signaling pathways reprogramming NF‐κB, MAPK, TGF‐β, JAK/STAT, PI3K/Akt/mTOR, NLRP3. Of note, despite increasing availability treatments for osteoarthritis, like intra‐articular injections, surgery, cellular therapy, demand more effective clinical therapies remained steady. Therefore, also describe current prospective therapeutic methods deem be a target, physical stimulus, chemical compounds, biological molecules, enhance cartilage repair alleviate progression

Language: Английский

---