International Journal of Extreme Manufacturing,
Journal Year:
2023,
Volume and Issue:
5(2), P. 025001 - 025001
Published: Feb. 20, 2023
Abstract
Three-dimensional
(3D)
printing
provides
a
promising
way
to
fabricate
biodegradable
scaffolds
with
designer
architectures
for
the
regeneration
of
various
tissues.
However,
existing
3D-printed
commonly
suffer
from
weak
cell-scaffold
interactions
and
insufficient
cell
organizations
due
limited
resolution
features.
Here,
composite
mechanically-robust
frameworks
aligned
nanofibrous
are
presented
hybrid
manufactured
by
combining
techniques
3D
printing,
electrospinning,
unidirectional
freeze-casting.
It
was
found
that
provided
volume-stable
environments
enabled
directed
cellular
infiltration
tissue
regeneration.
In
particular,
micropores
served
as
artificial
extracellular
matrix
materials
improved
attachment,
proliferation,
cells.
The
proposed
can
also
support
adipogenic
maturation
adipose-derived
stem
cells
(ADSCs)
in
vitro
.
Moreover,
were
guide
promote
nearby
neovascularization
when
implanted
into
subcutaneous
model
rats,
addition
ADSCs
further
enhanced
their
potential.
manufacturing
strategy
might
provide
produce
additional
topological
cues
within
better
Journal of Nanobiotechnology,
Journal Year:
2024,
Volume and Issue:
22(1)
Published: Jan. 31, 2024
Abstract
The
osteoimmune
microenvironment
induced
by
implants
plays
a
significant
role
in
bone
regeneration.
It
is
essential
to
efficiently
and
timely
switch
the
macrophage
phenotype
from
M1
M2
for
optimal
healing.
This
study
examined
impact
of
calcium
phosphate
(CaP)
coating
on
physiochemical
properties
highly
ordered
polycaprolactone
(PCL)
scaffolds
fabricated
using
melt
electrowritten
(MEW).
Additionally,
it
investigated
influence
these
polarization
their
immunomodulation
osteogenesis.
results
revealed
that
CaP
coated
PCL
scaffold
exhibited
rougher
surface
topography
higher
hydrophilicity
comparison
without
coating.
Besides,
morphology
release
Ca
2+
were
crucial
regulating
transition
macrophages
phenotypes.
They
might
activate
PI3K/AKT
cAMP-PKA
pathways,
respectively,
facilitate
polarization.
In
addition,
could
not
only
enhance
osteogenic
differentiation
marrow-derived
mesenchymal
stem
cells
(BMSCs)
vitro
but
also
promote
regeneration
vivo.
Taken
together,
can
be
employed
control
phenotypic
switching
macrophages,
thereby
creating
beneficial
immunomodulatory
promotes
Graphical
abstract
Small,
Journal Year:
2022,
Volume and Issue:
18(39)
Published: Aug. 28, 2022
Abstract
Precise
timing
of
macrophage
polarization
plays
a
pivotal
role
in
immunomodulation
tissue
regeneration,
yet
most
studies
mainly
focus
on
M2
macrophages
for
their
anti‐inflammatory
and
regenerative
effects
while
the
essential
proinflammatory
M1
phenotype
early
inflammation
stage
is
largely
underestimated.
Herein,
superparamagnetic
hydrogel
capable
timely
controlling
constructed
by
grafting
nanoparticles
collagen
nanofibers.
The
magnetic
responsive
network
enables
efficient
encapsulated
to
through
podosome/Rho/ROCK
mechanical
pathway
response
static
field
(MF)
as
needed.
Taking
advantage
remote
accessibility
together
with
hydrogels,
temporal
engineered
transition
course
preserving
at
healing,
well
enhancing
prohealing
effect
middle/late
stages
established
via
delayed
MF
switch.
Such
precise
matching
process
injured
eventually
leads
optimized
immunomodulatory
bone
healing
vivo.
Overall,
this
study
offers
remotely
time‐scheduled
approach
polarization,
which
manipulation
progression
during
healing.
Bone Research,
Journal Year:
2023,
Volume and Issue:
11(1)
Published: Dec. 20, 2023
The
skeleton
is
a
highly
innervated
organ
in
which
nerve
fibers
interact
with
various
skeletal
cells.
Peripheral
endings
release
neurogenic
factors
and
sense
signals,
mediate
bone
metabolism
pain.
In
recent
years,
tissue
engineering
has
increasingly
focused
on
the
effects
of
nervous
system
regeneration.
Simultaneous
regeneration
nerves
through
use
materials
or
by
enhancement
endogenous
repair
signals
been
proven
to
promote
functional
Additionally,
emerging
information
mechanisms
interoception
central
regulation
homeostasis
provide
an
opportunity
for
advancing
biomaterials.
However,
comprehensive
reviews
this
topic
are
lacking.
Therefore,
review
provides
overview
relationship
between
regeneration,
focusing
applications.
We
discuss
novel
regulatory
explore
innovative
approaches
based
nerve-bone
interactions
Finally,
challenges
future
prospects
field
briefly
discussed.
Advanced Functional Materials,
Journal Year:
2022,
Volume and Issue:
33(2)
Published: Nov. 4, 2022
Abstract
Effective
therapies
capable
of
simultaneously
inhibiting
inflammation
and
promoting
bone
healing
remain
to
be
developed
for
inflammatory
disease.
Stem
cell
hold
great
promise
a
variety
diseases,
but
their
translation
is
hampered
by
low
survival,
rapid
clearance,
limited
functional
integration
transplanted
stem
cells
in
target
tissues.
Herein,
multifunctional
hydrogel‐based
niche
engineering
strategy
reported
the
treatment
loss.
By
rationally
integrating
different
modules,
an
injectable
engineered,
which
possesses
temperature‐triggered
gelling
performance,
inflammation/oxidative
stress‐resolving
activity,
stem‐cell
binding
survival‐enhancing
capacity,
osteogenesis‐promoting
capability.
Using
ectomesenchymal
(EMSCs),
effectiveness
this
functionally
advanced
synthetic
demonstrated
rats
with
periodontitis,
representative
loss
Synergistic
effects
hydrogel
EMSCs
are
also
confirmed,
respect
normalizing
pathological
microenvironment
improving
alveolar
regeneration
periodontal
tissue.
Mechanistically,
osteogenic
differentiation
capacities
mainly
achieved
incorporated
nanotherapy
via
GDF15/Atf3/c‐Fos
axis
MAPK
signaling
pathway.
Besides
newly
engineered
hydrogel‐stem
promising
other
defects.
Polymers,
Journal Year:
2022,
Volume and Issue:
14(3), P. 445 - 445
Published: Jan. 22, 2022
The
design
of
scaffolds
with
optimal
biomechanical
properties
for
load-bearing
applications
is
an
important
topic
research.
Most
studies
have
addressed
this
problem
by
focusing
on
the
material
composition
and
not
coupled
effect
between
scaffold
architecture.
Polymer-bioglass
been
investigated
due
to
excellent
bioactivity
bioglass,
which
release
ions
that
activate
osteogenesis.
However,
preparation
methods
usually
require
use
organic
solvents
induce
surface
modifications
bioglass
particles,
compromising
adhesion
polymeric
thus
mechanical
properties.
In
paper,
we
used
a
simple
melt
blending
approach
produce
polycaprolactone/bioglass
pellets
construct
pore
size
gradient.
results
show
addition
particles
improved
and,
selected
architecture,
all
presented
in
cortical
bone
region.
Moreover,
indicated
positive
long-term
biological
performance
scaffolds.
gradient
also
induced
cell
spreading
Advanced Functional Materials,
Journal Year:
2023,
Volume and Issue:
33(16)
Published: Jan. 17, 2023
Abstract
Driving
endogenous
bone
regeneration
by
cell‐
and
factor‐free
biomaterials
is
the
most
ideal
repair
strategy.
Herein,
hybrid
interleaved
scaffold
(HDSH)
with
nanosized
interfacial
integration
assembled
organic/inorganic
interactive
bonding
at
nanoscale.
With
help
of
transcriptome
proteome
analysis,
regenerative
mechanism
this
elaborated
molecular
level,
which
confirms
that
strategy
recreates
a
suitable
immune
microenvironment
(anti‐inflammatory
M2‐polarizing)
drives
functional
cell
cytokine
adhesion,
as
well
inchoate
vascularization.
It
greatly
enhances
stem
recruitment,
subsequently
initiates
robust
vasculogenesis
osteogenesis.
Significant
bony
reconstitution
in
rabbit
cranial
defect
model
(Φ
=
10
mm)
observed
after
12
weeks,
realizes
completely
new
coverage
79%
breaking
load
strength
relative
to
natural
cranium.
By
enhancing
nano‐sized
integration,
can
provide
effective
guidance
for
developing
highly
bioactive
bone‐regenerative
implants.
