Artificial Nonenzymatic Antioxidant MXene Nanosheet-Anchored Injectable Hydrogel as a Mild Photothermal-Controlled Oxygen Release Platform for Diabetic Wound Healing

ACS Nano, Journal Year: 2022, Volume and Issue: 16(5), P. 7486 - 7502

Published: May 9, 2022

Hypoxia, excessive reactive oxygen species (ROS), impaired angiogenesis, lasting inflammation, and bacterial infection, are key problems impeding diabetic wound healing. Particularly, controllable release ROS scavenging capacities critical during the healing process. Here, an injectable hydrogel based on hyaluronic acid-graft-dopamine (HA-DA) polydopamine (PDA) coated Ti3C2 MXene nanosheets is developed catalytically cross-linked by oxyhemoglobin/hydrogen (HbO2/H2O2) system combined with mild photothermal stimulation for HbO2 not only acts as a horseradish peroxidase-like to catalyze formation but also carrier controllably when activated heat produced from near-infrared (NIR) irradiation. Specifically, can provide repeatedly binding in air NIR off. The stable photoresponsive heating behavior of ensures repeatable release. Additionally, artificial nonenzymatic antioxidant proposed scavenge nitrogen including H2O2, O2•-, •OH, keeping intracellular redox homeostasis alleviating oxidative stress, eradicate bacteria avoid infection. antibacterial abilities further improved PDA coating, which promotes cross-linking into network hydrogel. HA-DA molecules endow capacity regulate macrophage polarization M1 M2 achieve anti-inflammation. More importantly, MXene-anchored multifunctions tissue adhesion, self-healing, injectability, hemostasis, stimulation, greatly human umbilical vein endothelial cell proliferation migration notably facilitates infected

Language: Английский

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Extracellular vesicle-loaded hydrogels for tissue repair and regeneration

Materials Today Bio, Journal Year: 2022, Volume and Issue: 18, P. 100522 - 100522

Published: Dec. 21, 2022

Extracellular vesicles (EVs) are a collective term for nanoscale or microscale secreted by cells that play important biological roles. Mesenchymal stem class of with the potential self-healing and multidirectional differentiation. In recent years, numerous studies have shown EVs, especially those mesenchymal cells, can promote repair regeneration various tissues and, thus, significant in regenerative medicine. However, due to rapid clearance capacity circulatory system, EVs barely able act persistently at specific sites target tissues. Hydrogels good biocompatibility loose porous structural properties allow them serve as EV carriers, thereby prolonging retention certain areas slowing release EVs. When needed function sites, EV-loaded hydrogels stand an excellent approach. this review, we first introduce sources, roles, extraction characterization methods describe their current application status. We then review different types discuss factors influencing abilities carry summarize several strategies loading into characterizing hydrogels. Furthermore, applications tissue repair. This article concludes summary state research on outlook future directions, which hope will provide promising ideas researchers.

Language: Английский

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GelMA/PEGDA microneedles patch loaded with HUVECs-derived exosomes and Tazarotene promote diabetic wound healing

Journal of Nanobiotechnology, Journal Year: 2022, Volume and Issue: 20(1)

Published: March 19, 2022

Clinical work and research on diabetic wound repair remain challenging globally. Although various conventional dressings have been continuously developed, the efficacy is unsatisfactory. The effect of drug delivery limited by depth penetration. sustained release biomolecules from biological a promising treatment approach to healing. An assortment cell-derived exosomes (exos) proved be instrumental in tissue regeneration, researchers are dedicated developing carriers with unique properties. Herein, we reported methacrylate gelatin (GelMA) microneedles (MNs) patch achieve transdermal controlled exos tazarotene. Our MNs comprising GelMA/PEGDA hydrogel has distinctive features that maintain activity drugs vitro. Additionally, its physical structure prevents it being tightly attached skin wound, promotes cell migration, angiogenesis slowly releasing tazarotene deep layer skin. full-thickness cutaneous mouse model was carried out demonstrate therapeutic effects GelMA/PEGDA@T + patch. As result, our presents potentially valuable method for repairing clinical applications.

Language: Английский

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Probing and Manipulating Noncovalent Interactions in Functional Polymeric Systems

Chemical Reviews, Journal Year: 2022, Volume and Issue: 122(18), P. 14594 - 14678

Published: Sept. 2, 2022

Noncovalent interactions, which usually feature tunable strength, reversibility, and environmental adaptability, have been recognized as driving forces in a variety of biological chemical processes, contributing to the recognition between molecules, formation molecule clusters, establishment complex structures macromolecules. The marriage noncovalent interactions conventional covalent polymers offers systems novel mechanical, physicochemical, properties, are highly dependent on binding mechanisms that can be illuminated via quantification. This review systematically discusses nanomechanical characterization typical polymeric systems, mainly through direct force measurements at microscopic, nanoscopic, molecular levels, provide quantitative information (e.g., ranges, strengths, dynamics) behaviors. fundamental understandings intermolecular interfacial then correlated macroscopic performances series noncovalently bonded polymers, whose functions stimuli-responsiveness, self-healing capacity, universal adhesiveness) customized manipulation providing insights into rational design advanced materials with applications biomedical, energy, environmental, other engineering fields.

Language: Английский

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Silk fibroin-based biomaterials for cartilage/osteochondral repair

Theranostics, Journal Year: 2022, Volume and Issue: 12(11), P. 5103 - 5124

Published: Jan. 1, 2022

Osteoarthritis (OA) is a common joint disease with high disability rate.In addition, OA not only causes great physiological and psychological harm to patients, but also puts pressure on the social healthcare system.Pathologically, disintegration of cartilage lesions subchondral bone are related OA.Currently, tissue engineering, which expected overcome defects existing treatment methods, had lot research in field cartilage/osteochondral repair.Silk fibroin (SF), as natural macromolecular material good biocompatibility, unique mechanical properties, excellent processability degradability, holds potential engineering.Nowadays, SF been prepared into various materials adapt demands repair.SF-based biomaterials can be functionally modified enhance repair performance further.In this review, preparation types, structures, functional modifications SF-based used for summarized discussed.We hope that review will provide reference design development field.

Language: Английский

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Bioinspired polydopamine hydrogels: Strategies and applications

Progress in Polymer Science, Journal Year: 2023, Volume and Issue: 146, P. 101740 - 101740

Published: Sept. 17, 2023

Language: Английский

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Cell-Free Osteoarthritis Treatment with Sustained-Release of Chondrocyte-Targeting Exosomes from Umbilical Cord-Derived Mesenchymal Stem Cells to Rejuvenate Aging Chondrocytes

ACS Nano, Journal Year: 2023, Volume and Issue: 17(14), P. 13358 - 13376

Published: July 13, 2023

As chondrocytes from osteoarthritic cartilage usually exhibit aging and senescent characteristics, targeting could be a potential therapeutic strategy. In this study, exosomes derived umbilical cord-derived mesenchymal stem cells (UCMSC-EXOs) combined with the chondrocyte-targeting capacity controlled-release system were proposed for osteoarthritis (OA) treatment via rejuvenating chondrocytes. The essential functional miRNAs within UCMSC-EXOs investigated, p53 signaling pathway identified as key factor. To improve efficiency retention time of in vivo, (EXOs) engineered on membranes designed polymers, encapsulated thiolated hyaluronic acid microgels to form "two-phase" releasing system, which synergistically facilitated repair OA rat model. Together, study highlighted effects combine sustained-release strategies toward future cell-free treatment.

Language: Английский

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Dynamically Bioresponsive DNA Hydrogel Incorporated with Dual-Functional Stem Cells from Apical Papilla-Derived Exosomes Promotes Diabetic Bone Regeneration

ACS Applied Materials & Interfaces, Journal Year: 2022, Volume and Issue: 14(14), P. 16082 - 16099

Published: March 28, 2022

The regeneration of bone defects in patients with diabetes mellitus (DM) is remarkably impaired by hyperglycemia and over-expressed proinflammatory cytokines, proteinases (such as matrix metalloproteinases, MMPs), etc. In view the fact that exosomes represent a promising nanomaterial, herein, we reported excellent capacity stem cells from apical papilla-derived (SCAP-Exo) to facilitate angiogenesis osteogenesis whether normal or diabetic conditions vitro. Then, bioresponsive polyethylene glycol (PEG)/DNA hybrid hydrogel was developed support controllable release SCAP-Exo for defects. This system could be triggered elevated pathological cue (MMP-9) response dynamic microenvironment. It further confirmed administration injectable SCAP-Exo-loaded PEG/DNA into mandibular defect rats demonstrated great therapeutic effect on promoting vascularized regeneration. addition, miRNA sequencing suggested mechanism dual-functional might related highly expressed miRNA-126-5p miRNA-150-5p. Consequently, our study provides valuable insights design exosome-delivery systems improve patients.

Language: Английский

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Ultra-durable cell-free bioactive hydrogel with fast shape memory and on-demand drug release for cartilage regeneration

Nature Communications, Journal Year: 2023, Volume and Issue: 14(1)

Published: Nov. 27, 2023

Language: Английский

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Exosomes-loaded electroconductive nerve dressing for nerve regeneration and pain relief against diabetic peripheral nerve injury

Bioactive Materials, Journal Year: 2023, Volume and Issue: 26, P. 194 - 215

Published: March 4, 2023

Over the years, electroconductive hydrogels (ECHs) have been extensively applied for stimulating nerve regeneration and restoring locomotor function after peripheral injury (PNI) with diabetes, given their favorable mechanical electrical properties identical to endogenous tissue. Nevertheless, PNI causes loss of inflammatory pain, especially in diabetic patients. It has established that bone marrow stem cells-derived exosomes (BMSCs-Exos) analgesic, anti-inflammatory tissue properties. Herein, we designed an ECH loaded BMSCs-Exos (ECH-Exos) dressing treat achieve functional recovery pain relief. Given its potent adhesive self-healing properties, this laminar is convenient treatment damaged fibers by automatically wrapping around them form a size-matched tube-like structure, avoiding cumbersome implantation process. Our vitro studies showed ECH-Exos could facilitate attachment migration Schwann cells. Meanwhile, Exos system modulate M2 macrophage polarization via NF-κB pathway, thereby attenuating PNI. Additionally, enhanced myelinated axonal MEK/ERK pathway vivo, consequently ameliorating muscle denervation atrophy further promoting restoration. findings suggest huge prospects regeneration, restoration relief patients

Language: Английский

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