Microorganisms,
Journal Year:
2024,
Volume and Issue:
12(9), P. 1828 - 1828
Published: Sept. 4, 2024
Investigating
the
complex
interactions
between
microbiota
and
immunity
is
crucial
for
a
fruitful
understanding
progress
of
human
health
disease.
This
review
assesses
animal
models,
next-generation
in
vitro
silico
approaches
that
are
used
to
decipher
microbiome-immunity
axis,
evaluating
their
strengths
limitations.
While
models
provide
comprehensive
biological
context,
they
also
raise
ethical
practical
concerns.
Conversely,
modern
reduce
involvement
but
require
specific
costs
materials.
When
considering
environmental
impact
these
emerge
as
promising
resource
reduction,
robust
experimental
validation
ongoing
refinement.
Their
potential
significant,
paving
way
more
sustainable
future
research.
ACS Synthetic Biology,
Journal Year:
2025,
Volume and Issue:
unknown
Published: May 16, 2025
Creatine
is
a
popular
athletic
supplement
that
has
also
been
shown
to
improve
cognitive
performance
upon
sleep
deprivation.
However,
it
rapidly
cleared
from
the
gastrointestinal
tract
few
hours
after
consumption.
Toward
providing
persistent
creatine
dose,
we
engineered
human
probiotic
Escherichia
coli
Nissle
(EcN)
produce
guanidinoacetic
acid
(GAA),
which
converted
in
liver.
We
find
GAA-producing
enzymes
present
microbiome
and
compare
their
activities
known
enzymes.
Three
copies
of
arginine:glycine
amidinotransferase
(AGAT)
Actinokineospora
terrae
are
expressed
genome,
native
gcvP,
argR,
argA
edited
or
deleted
substrate
availability
without
negatively
impacting
cell
viability.
A
standard
EcN
dose
(1012
cells)
produces
41
±
7
mg
GAA
per
hour
under
laboratory
conditions.
This
work
demonstrates
bacterium
can
be
sustained
titers
impact
performance.
ACS Biomaterials Science & Engineering,
Journal Year:
2023,
Volume and Issue:
9(5), P. 2780 - 2792
Published: April 5, 2023
Cystic
fibrosis
(CF)
is
one
of
the
most
frequent
genetic
diseases,
caused
by
dysfunction
CF
transmembrane
conductance
regulator
(CFTR)
chloride
channel.
particularly
affects
epithelium
respiratory
system.
Therapies
aim
at
rescuing
CFTR
defects
in
epithelium,
but
heterogeneity
hinders
finding
a
single
and
generally
effective
treatment.
Therefore,
vitro
models
have
been
developed
to
study
guide
patient
therapy.
Here,
we
show
model
on-chip
coupling
feasibility
human
bronchial
differentiated
air–liquid
interface
innovation
microfluidics.
We
demonstrate
that
dynamic
flow
enhanced
cilia
distribution
increased
mucus
quantity,
thus
promoting
tissue
differentiation
short
time.
The
microfluidic
devices
highlighted
differences
between
non-CF
epithelia,
as
shown
electrophysiological
measures,
viscosity,
analysis
ciliary
beat
frequency.
described
may
be
handy
instrument
for
studying
setting
up
therapies.
As
proof
principle,
administrated
corrector
VX-809
observed
decrease
thickness
viscosity.
Tissue Barriers,
Journal Year:
2023,
Volume and Issue:
12(2)
Published: June 9, 2023
The
maintenance
of
body
homeostasis
relies
heavily
on
physiological
barriers.
Dysfunction
these
barriers
can
lead
to
various
pathological
processes,
including
increased
exposure
toxic
materials
and
microorganisms.
Various
methods
exist
investigate
barrier
function
in
vivo
vitro.
To
a
highly
reproducible
manner,
ethically,
high
throughput,
researchers
have
turned
non-animal
techniques
micro-scale
technologies.
In
this
comprehensive
review,
the
authors
summarize
current
applications
organ-on-a-chip
microfluidic
devices
study
review
covers
blood-brain
barrier,
ocular
barriers,
dermal
respiratory
intestinal,
hepatobiliary,
renal/bladder
under
both
healthy
conditions.
article
then
briefly
presents
placental/vaginal,
tumour/multi-organ
devices.
Finally,
discusses
Computational
Fluid
Dynamics
systems
that
integrate
biological
This
provides
concise
yet
informative
overview
state-of-the-art
studies
using
Food Frontiers,
Journal Year:
2024,
Volume and Issue:
5(3), P. 1031 - 1062
Published: March 5, 2024
Abstract
Much
of
nutrition
research
has
been
conventionally
based
on
the
use
simplistic
in
vitro
systems
or
animal
models,
which
have
extensively
employed
an
effort
to
better
understand
relationships
between
diet
and
complex
diseases
as
well
evaluate
food
safety.
Although
these
models
undeniably
contributed
increase
our
mechanistic
understanding
basic
biological
processes,
they
do
not
adequately
model
human
physiopathological
phenomena,
creating
concerns
about
translatability
humans.
During
last
decade,
extraordinary
advancement
stem
cell
culturing,
three‐dimensional
cultures,
sequencing
technologies,
computer
science
occurred,
originated
a
wealth
novel
human‐based
more
physiologically
relevant
tools.
These
tools,
also
known
“new
approach
methodologies,”
comprise
patient‐derived
organoids,
organs‐on‐chip,
multi‐omics
approach,
along
with
computational
analysis,
represent
innovative
exciting
tools
forward
from
human‐biology‐oriented
perspective.
After
considering
some
shortcomings
conventional
vivo
approaches,
here
we
describe
main
available
emerging
that
are
appropriate
for
designing
human‐relevant
research.
Our
aim
is
encourage
discussion
opportunity
explore
paths
promote
paradigm‐change
toward
biology‐focused
nutritional
pathophysiology,
products,
develop
effective
targeted
preventive
therapeutic
strategies
while
helping
reducing
number
replacing
animals
Microorganisms,
Journal Year:
2024,
Volume and Issue:
12(9), P. 1828 - 1828
Published: Sept. 4, 2024
Investigating
the
complex
interactions
between
microbiota
and
immunity
is
crucial
for
a
fruitful
understanding
progress
of
human
health
disease.
This
review
assesses
animal
models,
next-generation
in
vitro
silico
approaches
that
are
used
to
decipher
microbiome-immunity
axis,
evaluating
their
strengths
limitations.
While
models
provide
comprehensive
biological
context,
they
also
raise
ethical
practical
concerns.
Conversely,
modern
reduce
involvement
but
require
specific
costs
materials.
When
considering
environmental
impact
these
emerge
as
promising
resource
reduction,
robust
experimental
validation
ongoing
refinement.
Their
potential
significant,
paving
way
more
sustainable
future
research.
